d9e xp rabbit mab 4060  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc d9e xp rabbit mab 4060
    D9e Xp Rabbit Mab 4060, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    d9e xp 4060  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc d9e xp 4060
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    phospho akt ser473 d9e xp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473 d9e xp
    (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT <t>(Ser473)</t> in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).
    Phospho Akt Ser473 D9e Xp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "ATP citrate lyase is an essential player of the metabolic rewiring induced by PTEN loss during T-ALL development"

    Article Title: ATP citrate lyase is an essential player of the metabolic rewiring induced by PTEN loss during T-ALL development

    Journal: bioRxiv

    doi: 10.1101/2023.03.27.534353

    (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT (Ser473) in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).
    Figure Legend Snippet: (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT (Ser473) in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).

    Techniques Used: Mutagenesis, Western Blot, Isolation

    (A) Phospho AKT (ser473) and (B) and phospho ACLY (ser455) levels on DN, DP, SP4 and SP8 from pre-leukemic PTEN Δ/Δ mutant or PTEN flox2 control counterparts. In mutant mice, which carried the YFP flox2 reporter, DN, DP, SP4 and SP8 cells are gated on YFP+ cells. Levels of Bcl-xL (C) and BCL-2 (D) in DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals (E) Sensitivity of DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals to dexamethasone treatment ex vivo. (F) Percentages of DP cells in PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals receiving PBS or dexamethasone.
    Figure Legend Snippet: (A) Phospho AKT (ser473) and (B) and phospho ACLY (ser455) levels on DN, DP, SP4 and SP8 from pre-leukemic PTEN Δ/Δ mutant or PTEN flox2 control counterparts. In mutant mice, which carried the YFP flox2 reporter, DN, DP, SP4 and SP8 cells are gated on YFP+ cells. Levels of Bcl-xL (C) and BCL-2 (D) in DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals (E) Sensitivity of DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals to dexamethasone treatment ex vivo. (F) Percentages of DP cells in PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals receiving PBS or dexamethasone.

    Techniques Used: Mutagenesis, Ex Vivo

    anti phospho akt ser473 d9e  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho akt ser473 d9e
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    phospho akt ser473 d9e xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473 d9e xp rabbit mab
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    rabbit anti phospho akt ser473 d9e xp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti phospho akt ser473 d9e xp
    Rabbit Anti Phospho Akt Ser473 D9e Xp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho akt ser473 d9e xp rabbit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473 d9e xp rabbit
    Antibody used in the study
    Phospho Akt Ser473 D9e Xp Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Estrogen downregulates CD73/adenosine axis hyperactivity via adaptive modulation PI3K/Akt signaling to prevent myocarditis and arrhythmias during chronic catecholamines stress"

    Article Title: Estrogen downregulates CD73/adenosine axis hyperactivity via adaptive modulation PI3K/Akt signaling to prevent myocarditis and arrhythmias during chronic catecholamines stress

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-023-01052-0

    Antibody used in the study
    Figure Legend Snippet: Antibody used in the study

    Techniques Used:

    d9e xp rabbit mab cell signaling 4060 mek1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc d9e xp rabbit mab cell signaling 4060 mek1
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    phospho akt ser473 d9e xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho akt ser473 d9e xp rabbit mab
    Primary and secondary antibodies used for Western blot experiments.
    Phospho Akt Ser473 D9e Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice"

    Article Title: Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice

    Journal: Nutrients

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    Primary and secondary antibodies used for Western blot experiments.
    Figure Legend Snippet: Primary and secondary antibodies used for Western blot experiments.

    Techniques Used: Western Blot

    ser473 d9e rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ser473 d9e rabbit mab
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    Cell Signaling Technology Inc d9e xp rabbit mab 4060
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    (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT <t>(Ser473)</t> in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).
    Phospho Akt Ser473 D9e Xp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT <t>(Ser473)</t> in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).
    Anti Phospho Akt Ser473 D9e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT <t>(Ser473)</t> in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).
    Phospho Akt Ser473 D9e Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT <t>(Ser473)</t> in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).
    Rabbit Anti Phospho Akt Ser473 D9e Xp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT (Ser473) in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).

    Journal: bioRxiv

    Article Title: ATP citrate lyase is an essential player of the metabolic rewiring induced by PTEN loss during T-ALL development

    doi: 10.1101/2023.03.27.534353

    Figure Lengend Snippet: (A) Principal component analysis of 4 pten mutant and 5 pten flox2 thymi issued from pre-leukemic 8 weeks old mice subjected to unsupervised metabolic analysis. (B) Heatmaps of the 50 most deregulated metabolites and (C) analysis of citrate, coenzyme A, glutamine, a-ketoglutarate, acetyl-coA and fatty acids in pre-leukemic PTEN Δ/Δ thymocytes or control counterparts . (D) Representative western blot analysis of phosphorylated ACLY (Ser455) and phoshorylated AKT (Ser473) in thymi isolated from 8 weeks old, pre-leukemic PTEN Δ/Δ mutant mice or PTEN flox2 control counterparts. (E) Histograms depicting phosphorylated ACLY levels (ser455) in the thymi of pre-leukemic PTEN Δ/Δ mutant or control animals in 3 independent experiments (data are shown as average ± SE).

    Article Snippet: Protein samples were allowed to migrated on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad Cat. 4561094) and then transferred on nitrocellulose membranes (Bio-Rad Cat. 1704158), which were subsequently incubated overnight at 4°C with the following primary antibodies: anti ATP citrate lysase antibody D1X6P (Cell Signaling 13390S), Anti Phospho ATP citrate Lysase antibody Ser455 (Cell Signaling 4331S), anti PTEN antibody (Cell Signaling 9188S), Anti Phospho-Akt Ser473 (D9E) XP® (Cell Signaling 4060S), Anti AKT antibody (Cell signaling 9272S) and anti-actin antibody (Abcam ab-14128).

    Techniques: Mutagenesis, Western Blot, Isolation

    (A) Phospho AKT (ser473) and (B) and phospho ACLY (ser455) levels on DN, DP, SP4 and SP8 from pre-leukemic PTEN Δ/Δ mutant or PTEN flox2 control counterparts. In mutant mice, which carried the YFP flox2 reporter, DN, DP, SP4 and SP8 cells are gated on YFP+ cells. Levels of Bcl-xL (C) and BCL-2 (D) in DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals (E) Sensitivity of DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals to dexamethasone treatment ex vivo. (F) Percentages of DP cells in PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals receiving PBS or dexamethasone.

    Journal: bioRxiv

    Article Title: ATP citrate lyase is an essential player of the metabolic rewiring induced by PTEN loss during T-ALL development

    doi: 10.1101/2023.03.27.534353

    Figure Lengend Snippet: (A) Phospho AKT (ser473) and (B) and phospho ACLY (ser455) levels on DN, DP, SP4 and SP8 from pre-leukemic PTEN Δ/Δ mutant or PTEN flox2 control counterparts. In mutant mice, which carried the YFP flox2 reporter, DN, DP, SP4 and SP8 cells are gated on YFP+ cells. Levels of Bcl-xL (C) and BCL-2 (D) in DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals (E) Sensitivity of DP cells from PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals to dexamethasone treatment ex vivo. (F) Percentages of DP cells in PTEN Δ/Δ mutant, ACLY Δ/Δ mutant, PTEN Δ/Δ ;ACLY Δ/Δ double mutant mice or flox control animals receiving PBS or dexamethasone.

    Article Snippet: Protein samples were allowed to migrated on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad Cat. 4561094) and then transferred on nitrocellulose membranes (Bio-Rad Cat. 1704158), which were subsequently incubated overnight at 4°C with the following primary antibodies: anti ATP citrate lysase antibody D1X6P (Cell Signaling 13390S), Anti Phospho ATP citrate Lysase antibody Ser455 (Cell Signaling 4331S), anti PTEN antibody (Cell Signaling 9188S), Anti Phospho-Akt Ser473 (D9E) XP® (Cell Signaling 4060S), Anti AKT antibody (Cell signaling 9272S) and anti-actin antibody (Abcam ab-14128).

    Techniques: Mutagenesis, Ex Vivo

    Antibody used in the study

    Journal: Cell Communication and Signaling : CCS

    Article Title: Estrogen downregulates CD73/adenosine axis hyperactivity via adaptive modulation PI3K/Akt signaling to prevent myocarditis and arrhythmias during chronic catecholamines stress

    doi: 10.1186/s12964-023-01052-0

    Figure Lengend Snippet: Antibody used in the study

    Article Snippet: 3 , Phospho-Akt (Ser473) (D9E) XP® Rabbit , Cell Signalling Technology , 4060S.

    Techniques: