Structured Review

Santa Cruz Biotechnology monoclonal α d1r
Monoclonal α D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal α d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
monoclonal α d1r - by Bioz Stars, 2023-11
86/100 stars

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Structured Review

Santa Cruz Biotechnology anti d1r antibody
Anti D1r Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d1r antibody/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti d1r antibody - by Bioz Stars, 2023-11
86/100 stars

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Structured Review

Santa Cruz Biotechnology d1r
A–D: primary striatal neurons were transfected or not (Control) with Cont siRNA, <t>D1R</t> siRNA and D2R siRNA. After 72 hours, mRNAs were extracted followed by RT-PCR (A and B) and protein expression levels detected by immunoblot (C and D). A: RT-PCR of D1R gene and, B: RT-PCR D2R. Note the presence of both D2R long and short isoforms. For A and B: ARP gene was used as an housekeeping gene. B and D: immunoblot using specific anti-D1R (C), and specific D2R (D) antibodies. β-tubulin was used for control loading.
D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
d1r - by Bioz Stars, 2023-11
86/100 stars

Images

1) Product Images from "Dopamine D2 Receptor Stimulation Potentiates PolyQ-Huntingtin-Induced Mouse Striatal Neuron Dysfunctions via Rho/ROCK-II Activation"

Article Title: Dopamine D2 Receptor Stimulation Potentiates PolyQ-Huntingtin-Induced Mouse Striatal Neuron Dysfunctions via Rho/ROCK-II Activation

Journal: PLoS ONE

doi: 10.1371/journal.pone.0008287

A–D: primary striatal neurons were transfected or not (Control) with Cont siRNA, D1R siRNA and D2R siRNA. After 72 hours, mRNAs were extracted followed by RT-PCR (A and B) and protein expression levels detected by immunoblot (C and D). A: RT-PCR of D1R gene and, B: RT-PCR D2R. Note the presence of both D2R long and short isoforms. For A and B: ARP gene was used as an housekeeping gene. B and D: immunoblot using specific anti-D1R (C), and specific D2R (D) antibodies. β-tubulin was used for control loading.
Figure Legend Snippet: A–D: primary striatal neurons were transfected or not (Control) with Cont siRNA, D1R siRNA and D2R siRNA. After 72 hours, mRNAs were extracted followed by RT-PCR (A and B) and protein expression levels detected by immunoblot (C and D). A: RT-PCR of D1R gene and, B: RT-PCR D2R. Note the presence of both D2R long and short isoforms. For A and B: ARP gene was used as an housekeeping gene. B and D: immunoblot using specific anti-D1R (C), and specific D2R (D) antibodies. β-tubulin was used for control loading.

Techniques Used: Transfection, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot


Structured Review

Santa Cruz Biotechnology d1r
A. Semiquantitative mRNA levels for <t>D1R</t> in cg1 . The graph depicts a decrease in all groups when compared to control. Low, medium and high AS groups show 92 ± 2.4, 52 ± 1.3 and 62 ± 0.6%, respectively considering the control group as 100%. The lowest mRNA level is in the medium AS group. Image B shows mRNA levels for D2R in the same region with 79 ± 1.2, 120 ± 1.5 and 129 ± 1.6% for groups with low, medium and high AS, respectively. Notice that there is a progressive increase in mRNA levels, as the autotomy behaviour increases (n = 4 in all groups). All percentages are significantly different when compared to control (one-way ANOVA, Bonferroni post hoc *p < 0.05). At the top of each frame, there is an example of the electrophoresis agarose gel showing the control oligonucleotide (upper band) and the cDNA of D1R's in (A) and D2R's in (B) (lower band).
D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
d1r - by Bioz Stars, 2023-11
86/100 stars

Images

1) Product Images from "Expression of the dopaminergic D1 and D2 receptors in the anterior cingulate cortex in a model of neuropathic pain"

Article Title: Expression of the dopaminergic D1 and D2 receptors in the anterior cingulate cortex in a model of neuropathic pain

Journal: Molecular Pain

doi: 10.1186/1744-8069-7-97

A. Semiquantitative mRNA levels for D1R in cg1 . The graph depicts a decrease in all groups when compared to control. Low, medium and high AS groups show 92 ± 2.4, 52 ± 1.3 and 62 ± 0.6%, respectively considering the control group as 100%. The lowest mRNA level is in the medium AS group. Image B shows mRNA levels for D2R in the same region with 79 ± 1.2, 120 ± 1.5 and 129 ± 1.6% for groups with low, medium and high AS, respectively. Notice that there is a progressive increase in mRNA levels, as the autotomy behaviour increases (n = 4 in all groups). All percentages are significantly different when compared to control (one-way ANOVA, Bonferroni post hoc *p < 0.05). At the top of each frame, there is an example of the electrophoresis agarose gel showing the control oligonucleotide (upper band) and the cDNA of D1R's in (A) and D2R's in (B) (lower band).
Figure Legend Snippet: A. Semiquantitative mRNA levels for D1R in cg1 . The graph depicts a decrease in all groups when compared to control. Low, medium and high AS groups show 92 ± 2.4, 52 ± 1.3 and 62 ± 0.6%, respectively considering the control group as 100%. The lowest mRNA level is in the medium AS group. Image B shows mRNA levels for D2R in the same region with 79 ± 1.2, 120 ± 1.5 and 129 ± 1.6% for groups with low, medium and high AS, respectively. Notice that there is a progressive increase in mRNA levels, as the autotomy behaviour increases (n = 4 in all groups). All percentages are significantly different when compared to control (one-way ANOVA, Bonferroni post hoc *p < 0.05). At the top of each frame, there is an example of the electrophoresis agarose gel showing the control oligonucleotide (upper band) and the cDNA of D1R's in (A) and D2R's in (B) (lower band).

Techniques Used: Electrophoresis, Agarose Gel Electrophoresis

A. This figure shows semiquantitative mRNA levels for D1R in cg2 . Notice an increase in all groups when compared to control. Low, medium and high AS groups show 121 ± 1.1, 269 ± 3.3 and 207 ± 1%, respectively considering the control group as 100%. The greatest increase is in the medium AS group. Image B shows mRNA levels for D2R in the same region with 71 ± 2.6, 44 ± 1.8 and 71 ± 0.8% for groups with low, medium and high AS, respectively. Also, notice that the lowest value is for the medium AS group (n = 4 in all groups). All percentages are significantly different when compared to control (one-way ANOVA, Bonferroni post hoc *p < 0.05). At the top of each frame, there is an example of the electrophoresis agarose gel showing the control oligonucleotide (upper band) and the cDNA of D1R's in (A) and D2R's in (B) (lower band).
Figure Legend Snippet: A. This figure shows semiquantitative mRNA levels for D1R in cg2 . Notice an increase in all groups when compared to control. Low, medium and high AS groups show 121 ± 1.1, 269 ± 3.3 and 207 ± 1%, respectively considering the control group as 100%. The greatest increase is in the medium AS group. Image B shows mRNA levels for D2R in the same region with 71 ± 2.6, 44 ± 1.8 and 71 ± 0.8% for groups with low, medium and high AS, respectively. Also, notice that the lowest value is for the medium AS group (n = 4 in all groups). All percentages are significantly different when compared to control (one-way ANOVA, Bonferroni post hoc *p < 0.05). At the top of each frame, there is an example of the electrophoresis agarose gel showing the control oligonucleotide (upper band) and the cDNA of D1R's in (A) and D2R's in (B) (lower band).

Techniques Used: Electrophoresis, Agarose Gel Electrophoresis

Immuno Dot performed for D1R in control animals in the cerebral cortex . This control was done in order to validate the experimental procedure for dopamine D1 receptors in the ACC and the specificity of the antibody. The (A) column shows the blocking D1R peptide without primary antibody, (B) shows the blocking D1R peptide with D1R antibody and (C) the result in the cerebral cortex of control animals. The concentrations shown are the ones with positivity; the results were negative from 10 -3 .
Figure Legend Snippet: Immuno Dot performed for D1R in control animals in the cerebral cortex . This control was done in order to validate the experimental procedure for dopamine D1 receptors in the ACC and the specificity of the antibody. The (A) column shows the blocking D1R peptide without primary antibody, (B) shows the blocking D1R peptide with D1R antibody and (C) the result in the cerebral cortex of control animals. The concentrations shown are the ones with positivity; the results were negative from 10 -3 .

Techniques Used: Blocking Assay


Structured Review

Santa Cruz Biotechnology d1r
Sequences of PCR primers.
D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
d1r - by Bioz Stars, 2023-11
86/100 stars

Images

1) Product Images from "Dopamine Receptors Modulate Cytotoxicity of Natural Killer Cells via cAMP-PKA-CREB Signaling Pathway"

Article Title: Dopamine Receptors Modulate Cytotoxicity of Natural Killer Cells via cAMP-PKA-CREB Signaling Pathway

Journal: PLoS ONE

doi: 10.1371/journal.pone.0065860

Sequences of PCR primers.
Figure Legend Snippet: Sequences of PCR primers.

Techniques Used:

NK cells from mouse spleen were purified and detected for expression of D1R, D2R, D3R, D4R and D5R at mRNA and protein levels by using real time-PCR and Western blot, respectively. (A) The data was normalized to β-actin and represent M ± SD of three repeated experiments. (B) The electrophoretic bands show expression of the five DA receptor subtypes in NK cells and the molecular weights are 74, 51, 44, 53 and 53 kDa for D1R, D2R, D3R, D4R and D5R, respectively, which are consistent with those predicted.
Figure Legend Snippet: NK cells from mouse spleen were purified and detected for expression of D1R, D2R, D3R, D4R and D5R at mRNA and protein levels by using real time-PCR and Western blot, respectively. (A) The data was normalized to β-actin and represent M ± SD of three repeated experiments. (B) The electrophoretic bands show expression of the five DA receptor subtypes in NK cells and the molecular weights are 74, 51, 44, 53 and 53 kDa for D1R, D2R, D3R, D4R and D5R, respectively, which are consistent with those predicted.

Techniques Used: Purification, Expressing, Real-time Polymerase Chain Reaction, Western Blot

NK cells were treated with D1-like receptor agonist SKF38393 (10 −8 or 10 −7 M) or with antagonist SCH23390 (10 −7 M) plus SKF38393 for 4 h. Expression of D1R and D5R and level of CREB phosphorylation were detected by Western blot, and cAMP content in NK cells was measured by radioimmunoassay. (A) Representive electrophoretic bands and statistic chart of three independent experiments for D1R and D5R expression in NK cells. (B) The data showing cAMP content in NK cells were obtained from eight separate samples for each treatment. (C) Representive electrophoretic bands and statistic graph of three respective experiments for phosphorylated CREB level in NK cells. *p<0.05, **p<0.01, vs. control; + p<0.05, ++ p<0.01, vs. SKF (SKF38393). SCH: SCH23390.
Figure Legend Snippet: NK cells were treated with D1-like receptor agonist SKF38393 (10 −8 or 10 −7 M) or with antagonist SCH23390 (10 −7 M) plus SKF38393 for 4 h. Expression of D1R and D5R and level of CREB phosphorylation were detected by Western blot, and cAMP content in NK cells was measured by radioimmunoassay. (A) Representive electrophoretic bands and statistic chart of three independent experiments for D1R and D5R expression in NK cells. (B) The data showing cAMP content in NK cells were obtained from eight separate samples for each treatment. (C) Representive electrophoretic bands and statistic graph of three respective experiments for phosphorylated CREB level in NK cells. *p<0.05, **p<0.01, vs. control; + p<0.05, ++ p<0.01, vs. SKF (SKF38393). SCH: SCH23390.

Techniques Used: Expressing, Western Blot, RIA Assay


Structured Review

Santa Cruz Biotechnology goat polyclonal anti d1r
A–C: <t>D1R</t> immunoreactivity in luteal and endothelial (arrows) cells. D–F: D3R immunoreactivity in luteal and endothelial (arrows) cells. G–I: DA immunosignals in luteal cells; endothelial cells (arrows) are immunonegative. Scale bars = 20 µm.
Goat Polyclonal Anti D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat polyclonal anti d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
goat polyclonal anti d1r - by Bioz Stars, 2023-11
86/100 stars

Images

1) Product Images from "Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea"

Article Title: Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

Journal: PLoS ONE

doi: 10.1371/journal.pone.0104797

A–C: D1R immunoreactivity in luteal and endothelial (arrows) cells. D–F: D3R immunoreactivity in luteal and endothelial (arrows) cells. G–I: DA immunosignals in luteal cells; endothelial cells (arrows) are immunonegative. Scale bars = 20 µm.
Figure Legend Snippet: A–C: D1R immunoreactivity in luteal and endothelial (arrows) cells. D–F: D3R immunoreactivity in luteal and endothelial (arrows) cells. G–I: DA immunosignals in luteal cells; endothelial cells (arrows) are immunonegative. Scale bars = 20 µm.

Techniques Used:

Insert: D1R/D3R ratio. Data are expressed in arbitrary units; values are the means ± SD of thirty replicates; np: not present; different letters above the bars indicate significant different values (P<0.01).
Figure Legend Snippet: Insert: D1R/D3R ratio. Data are expressed in arbitrary units; values are the means ± SD of thirty replicates; np: not present; different letters above the bars indicate significant different values (P<0.01).

Techniques Used:

D1R (photo A) and D3R (photo B) immunosignals are localized in the cytoplasm of neuronal cells (NC) of the brain striatum. Note the immunonegativity of striosomes (S). Scale bars: photo A, C, E = 200 µm; B, D = 50 µm.
Figure Legend Snippet: D1R (photo A) and D3R (photo B) immunosignals are localized in the cytoplasm of neuronal cells (NC) of the brain striatum. Note the immunonegativity of striosomes (S). Scale bars: photo A, C, E = 200 µm; B, D = 50 µm.

Techniques Used:

Immunoblot showing D1R and D3R in anterior pituitary (negative control), CL, and brain striatum (positive control) lysates.
Figure Legend Snippet: Immunoblot showing D1R and D3R in anterior pituitary (negative control), CL, and brain striatum (positive control) lysates.

Techniques Used: Western Blot, Negative Control, Positive Control

Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with DA (100 nM), D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D1R antagonist (D1Rant, SCH 23390 hydrochloride, 1 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), and D3R antagonist (D3Rant, GR 103691, 1 nM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.
Figure Legend Snippet: Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with DA (100 nM), D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D1R antagonist (D1Rant, SCH 23390 hydrochloride, 1 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), and D3R antagonist (D3Rant, GR 103691, 1 nM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.

Techniques Used: Incubation

Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with, D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), EP2 antagonist (EP2ant, AH 6809, 1 µM); EP4 antagonist (EP4ant, AH 23848, 1 µM), and FP antagonist (FPant, AL 8810, 1 µM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.
Figure Legend Snippet: Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with, D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), EP2 antagonist (EP2ant, AH 6809, 1 µM); EP4 antagonist (EP4ant, AH 23848, 1 µM), and FP antagonist (FPant, AL 8810, 1 µM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.

Techniques Used: Incubation


Structured Review

Santa Cruz Biotechnology anti d1r
Anti D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti d1r - by Bioz Stars, 2023-11
86/100 stars

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Structured Review

Santa Cruz Biotechnology anti d1r
<t>D1R</t> (A), DAT (B), and TH (C) levels were quantified in the NAc of rats different from AMPH injection for saline ( p < 0.05). # Indicates significant difference from AMPH/UH group ( p < 0.05). Correlations between the percentage of the time spent in the AMPH-associated compartment in the CPP paradigm and D1R (D), DAT (E), and TH (F) in the animals that were exposed to tactile stimulation (TS) or unhandled (UH) during drug withdrawal. AMPH, amphetamine; D1R, dopamine 1 receptor; DAT, dopamine transporter; TH, tyrosine hydroxylase; UH, unhandled; TS, tactile stimulation; CPP, compartment place preference
Anti D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti d1r - by Bioz Stars, 2023-11
86/100 stars

Images

1) Product Images from "Tactile Stimulation in Adult Rats Modulates Dopaminergic Molecular Parameters in the Nucleus accumbens Preventing Amphetamine Relapse"

Article Title: Tactile Stimulation in Adult Rats Modulates Dopaminergic Molecular Parameters in the Nucleus accumbens Preventing Amphetamine Relapse

Journal: Molecular Neurobiology

doi: 10.1007/s12035-022-02927-y

D1R (A), DAT (B), and TH (C) levels were quantified in the NAc of rats different from AMPH injection for saline ( p < 0.05). # Indicates significant difference from AMPH/UH group ( p < 0.05). Correlations between the percentage of the time spent in the AMPH-associated compartment in the CPP paradigm and D1R (D), DAT (E), and TH (F) in the animals that were exposed to tactile stimulation (TS) or unhandled (UH) during drug withdrawal. AMPH, amphetamine; D1R, dopamine 1 receptor; DAT, dopamine transporter; TH, tyrosine hydroxylase; UH, unhandled; TS, tactile stimulation; CPP, compartment place preference
Figure Legend Snippet: D1R (A), DAT (B), and TH (C) levels were quantified in the NAc of rats different from AMPH injection for saline ( p < 0.05). # Indicates significant difference from AMPH/UH group ( p < 0.05). Correlations between the percentage of the time spent in the AMPH-associated compartment in the CPP paradigm and D1R (D), DAT (E), and TH (F) in the animals that were exposed to tactile stimulation (TS) or unhandled (UH) during drug withdrawal. AMPH, amphetamine; D1R, dopamine 1 receptor; DAT, dopamine transporter; TH, tyrosine hydroxylase; UH, unhandled; TS, tactile stimulation; CPP, compartment place preference

Techniques Used: Injection


Structured Review

Santa Cruz Biotechnology glp 1r d 6
List of Western blot antibodies
Glp 1r D 6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glp 1r d 6/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
glp 1r d 6 - by Bioz Stars, 2023-11
94/100 stars

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1) Product Images from "Liraglutide preserves CD34 + stem cells from dysfunction Induced by high glucose exposure"

Article Title: Liraglutide preserves CD34 + stem cells from dysfunction Induced by high glucose exposure

Journal: Cardiovascular Diabetology

doi: 10.1186/s12933-022-01486-9


Figure Legend Snippet: List of Western blot antibodies

Techniques Used: Western Blot


Structured Review

Santa Cruz Biotechnology anti d1r
Anti D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d1r/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti d1r - by Bioz Stars, 2023-11
86/100 stars

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    Santa Cruz Biotechnology monoclonal α d1r
    Monoclonal α D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal α d1r/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal α d1r - by Bioz Stars, 2023-11
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology anti d1r antibody
    Anti D1r Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti d1r antibody/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti d1r antibody - by Bioz Stars, 2023-11
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology d1r
    A–D: primary striatal neurons were transfected or not (Control) with Cont siRNA, <t>D1R</t> siRNA and D2R siRNA. After 72 hours, mRNAs were extracted followed by RT-PCR (A and B) and protein expression levels detected by immunoblot (C and D). A: RT-PCR of D1R gene and, B: RT-PCR D2R. Note the presence of both D2R long and short isoforms. For A and B: ARP gene was used as an housekeeping gene. B and D: immunoblot using specific anti-D1R (C), and specific D2R (D) antibodies. β-tubulin was used for control loading.
    D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d1r/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d1r - by Bioz Stars, 2023-11
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology goat polyclonal anti d1r
    A–C: <t>D1R</t> immunoreactivity in luteal and endothelial (arrows) cells. D–F: D3R immunoreactivity in luteal and endothelial (arrows) cells. G–I: DA immunosignals in luteal cells; endothelial cells (arrows) are immunonegative. Scale bars = 20 µm.
    Goat Polyclonal Anti D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti d1r/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat polyclonal anti d1r - by Bioz Stars, 2023-11
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    86
    Santa Cruz Biotechnology anti d1r
    A–C: <t>D1R</t> immunoreactivity in luteal and endothelial (arrows) cells. D–F: D3R immunoreactivity in luteal and endothelial (arrows) cells. G–I: DA immunosignals in luteal cells; endothelial cells (arrows) are immunonegative. Scale bars = 20 µm.
    Anti D1r, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti d1r/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti d1r - by Bioz Stars, 2023-11
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    Santa Cruz Biotechnology glp 1r d 6
    List of Western blot antibodies
    Glp 1r D 6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glp 1r d 6/product/Santa Cruz Biotechnology
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    A–D: primary striatal neurons were transfected or not (Control) with Cont siRNA, D1R siRNA and D2R siRNA. After 72 hours, mRNAs were extracted followed by RT-PCR (A and B) and protein expression levels detected by immunoblot (C and D). A: RT-PCR of D1R gene and, B: RT-PCR D2R. Note the presence of both D2R long and short isoforms. For A and B: ARP gene was used as an housekeeping gene. B and D: immunoblot using specific anti-D1R (C), and specific D2R (D) antibodies. β-tubulin was used for control loading.

    Journal: PLoS ONE

    Article Title: Dopamine D2 Receptor Stimulation Potentiates PolyQ-Huntingtin-Induced Mouse Striatal Neuron Dysfunctions via Rho/ROCK-II Activation

    doi: 10.1371/journal.pone.0008287

    Figure Lengend Snippet: A–D: primary striatal neurons were transfected or not (Control) with Cont siRNA, D1R siRNA and D2R siRNA. After 72 hours, mRNAs were extracted followed by RT-PCR (A and B) and protein expression levels detected by immunoblot (C and D). A: RT-PCR of D1R gene and, B: RT-PCR D2R. Note the presence of both D2R long and short isoforms. For A and B: ARP gene was used as an housekeeping gene. B and D: immunoblot using specific anti-D1R (C), and specific D2R (D) antibodies. β-tubulin was used for control loading.

    Article Snippet: After blocking in 5% nonfat dry milk (for ROCK-I and ROCK-II) or 5% BSA (Bovine Serum Albumin, SIGMA) (for D1R and D2R immunobloting) in TBS for 1 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies, rabbit polyclonal anti-ROCK-I anti-ROCK-II (1/500, Santa Cruz), mouse monoclonal anti-D1R (1/250, Millipore), rabbit polyclonal anti-D2R (1/1000, Millipore) and mouse monoclonal anti-β-tubulin (1/5000,Sigma), and revealed with appropriate anti-rabbit or anti-mouse peroxidase-conjugated secondary antibodies (1/5000, Jackson ImmunoResearch Laboratories) and the enhanced chemiluminescent (ECL) detection system (Pierce).

    Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot

    A–C: D1R immunoreactivity in luteal and endothelial (arrows) cells. D–F: D3R immunoreactivity in luteal and endothelial (arrows) cells. G–I: DA immunosignals in luteal cells; endothelial cells (arrows) are immunonegative. Scale bars = 20 µm.

    Journal: PLoS ONE

    Article Title: Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

    doi: 10.1371/journal.pone.0104797

    Figure Lengend Snippet: A–C: D1R immunoreactivity in luteal and endothelial (arrows) cells. D–F: D3R immunoreactivity in luteal and endothelial (arrows) cells. G–I: DA immunosignals in luteal cells; endothelial cells (arrows) are immunonegative. Scale bars = 20 µm.

    Article Snippet: Goat polyclonal anti-D1R (sc-31479), -D3R (sc-7525) -D5R (sc-1441), mouse monoclonal anti-D4R (sc-13169), and -D2R (sc-5303) primary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-DA primary antibody was purchased from Thermo Scientific (Fremont, CA, USA).

    Techniques:

    Insert: D1R/D3R ratio. Data are expressed in arbitrary units; values are the means ± SD of thirty replicates; np: not present; different letters above the bars indicate significant different values (P<0.01).

    Journal: PLoS ONE

    Article Title: Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

    doi: 10.1371/journal.pone.0104797

    Figure Lengend Snippet: Insert: D1R/D3R ratio. Data are expressed in arbitrary units; values are the means ± SD of thirty replicates; np: not present; different letters above the bars indicate significant different values (P<0.01).

    Article Snippet: Goat polyclonal anti-D1R (sc-31479), -D3R (sc-7525) -D5R (sc-1441), mouse monoclonal anti-D4R (sc-13169), and -D2R (sc-5303) primary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-DA primary antibody was purchased from Thermo Scientific (Fremont, CA, USA).

    Techniques:

    D1R (photo A) and D3R (photo B) immunosignals are localized in the cytoplasm of neuronal cells (NC) of the brain striatum. Note the immunonegativity of striosomes (S). Scale bars: photo A, C, E = 200 µm; B, D = 50 µm.

    Journal: PLoS ONE

    Article Title: Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

    doi: 10.1371/journal.pone.0104797

    Figure Lengend Snippet: D1R (photo A) and D3R (photo B) immunosignals are localized in the cytoplasm of neuronal cells (NC) of the brain striatum. Note the immunonegativity of striosomes (S). Scale bars: photo A, C, E = 200 µm; B, D = 50 µm.

    Article Snippet: Goat polyclonal anti-D1R (sc-31479), -D3R (sc-7525) -D5R (sc-1441), mouse monoclonal anti-D4R (sc-13169), and -D2R (sc-5303) primary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-DA primary antibody was purchased from Thermo Scientific (Fremont, CA, USA).

    Techniques:

    Immunoblot showing D1R and D3R in anterior pituitary (negative control), CL, and brain striatum (positive control) lysates.

    Journal: PLoS ONE

    Article Title: Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

    doi: 10.1371/journal.pone.0104797

    Figure Lengend Snippet: Immunoblot showing D1R and D3R in anterior pituitary (negative control), CL, and brain striatum (positive control) lysates.

    Article Snippet: Goat polyclonal anti-D1R (sc-31479), -D3R (sc-7525) -D5R (sc-1441), mouse monoclonal anti-D4R (sc-13169), and -D2R (sc-5303) primary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-DA primary antibody was purchased from Thermo Scientific (Fremont, CA, USA).

    Techniques: Western Blot, Negative Control, Positive Control

    Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with DA (100 nM), D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D1R antagonist (D1Rant, SCH 23390 hydrochloride, 1 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), and D3R antagonist (D3Rant, GR 103691, 1 nM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.

    Journal: PLoS ONE

    Article Title: Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

    doi: 10.1371/journal.pone.0104797

    Figure Lengend Snippet: Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with DA (100 nM), D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D1R antagonist (D1Rant, SCH 23390 hydrochloride, 1 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), and D3R antagonist (D3Rant, GR 103691, 1 nM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.

    Article Snippet: Goat polyclonal anti-D1R (sc-31479), -D3R (sc-7525) -D5R (sc-1441), mouse monoclonal anti-D4R (sc-13169), and -D2R (sc-5303) primary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-DA primary antibody was purchased from Thermo Scientific (Fremont, CA, USA).

    Techniques: Incubation

    Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with, D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), EP2 antagonist (EP2ant, AH 6809, 1 µM); EP4 antagonist (EP4ant, AH 23848, 1 µM), and FP antagonist (FPant, AL 8810, 1 µM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.

    Journal: PLoS ONE

    Article Title: Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

    doi: 10.1371/journal.pone.0104797

    Figure Lengend Snippet: Early (upper panel), mid (middle panel B), and late (bottom panel) CL incubated with, D1R agonist (D1Rag, dihydrexidine hydrochloride, 20 nM), D3R agonist (D3Rag, 7-hydroxy-PIPAT maleate, 2 nM), EP2 antagonist (EP2ant, AH 6809, 1 µM); EP4 antagonist (EP4ant, AH 23848, 1 µM), and FP antagonist (FPant, AL 8810, 1 µM). Values are the means ± SD of ten replicates. Asterisks indicate significant different values (P<0.01) versus control.

    Article Snippet: Goat polyclonal anti-D1R (sc-31479), -D3R (sc-7525) -D5R (sc-1441), mouse monoclonal anti-D4R (sc-13169), and -D2R (sc-5303) primary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-DA primary antibody was purchased from Thermo Scientific (Fremont, CA, USA).

    Techniques: Incubation

    Journal: Cardiovascular Diabetology

    Article Title: Liraglutide preserves CD34 + stem cells from dysfunction Induced by high glucose exposure

    doi: 10.1186/s12933-022-01486-9

    Figure Lengend Snippet: List of Western blot antibodies

    Article Snippet: GLP-1R (D-6) , Sc-390774 (Santa Cruz Biotecnology) , Mouse , 1:200.

    Techniques: Western Blot