d1 receptor expression (Millipore)
Structured Review

D1 Receptor Expression, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1 receptor expression/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Early life stress impairs VTA coordination of BLA network and behavioral states"
Article Title: Early life stress impairs VTA coordination of BLA network and behavioral states
Journal: bioRxiv
doi: 10.1101/2023.09.16.558081

Figure Legend Snippet: ( A ) Representative 20X image for CNT (Left) and ELS (Right) BLA slice, outlined in white. DAPI: blue, D1 receptor (D1R) immunoreactivity: green. ( B ) Summary plot shows mean BLA nuclei count in CNT (N = 5 animals, n = 6 slice per animal) and ELS (N = 4 animals, n = 6 slice per animal). ( C ) Summary plot shows mean optical density of BLA D1Rs for CNT and ELS animals. ( D ) Left: Representative images of coronal hindbrain slices for a CNT (Top) and ELS (Bottom) animal at 4X. Right: 20x images of outlined rectangles on 4X images with arrow-tips indicating tdT-TH+ colocalized neurons; VTA: white square; substantia nigra (SN): white rectangle. ( E ) Top: Grouped bar plot shows average number of BLA-projecting neurons (tdT+) from SN and VTA. Bottom: Grouped bar plot shows average number of TH+ neurons in SN and VTA. ( F ) Grouped bar plot shows percentage of tdT-TH+ colocalized neurons in SN and VTA. ** p < 0.01. Error bars represent SEM.
Techniques Used:
d1r (Millipore)
Structured Review

D1r, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dopamine D2 receptor on CD4 + T cells is protective against inflammatory responses and signs in a mouse model of rheumatoid arthritis"
Article Title: Dopamine D2 receptor on CD4 + T cells is protective against inflammatory responses and signs in a mouse model of rheumatoid arthritis
Journal: Arthritis Research & Therapy
doi: 10.1186/s13075-023-03071-1

Figure Legend Snippet: Effects of global D1r or D2r ablation on differentiation and function of CD4 + T cells in CIA mice. A Protein expression of D1R and D2R in ankle joints and spleen of mice. The left panel indicates Western blotting bands and the right panel is densitometric data normalized to β-actin ( n = 5/group). B Flow cytometric assay of the CD4 + T cell subsets, CD4 + IFN-γ + (Th1) cells, CD4 + IL-4 + (Th2) cells, CD4 + IL-17 + (Th17) cells, and CD4 + CD25 + Foxp3 + (Treg) cells. Representative images of the flow cytometric assay are exhibited in the left panel and quantitative data showing percentages of the CD4 + T cell subsets in total CD4 + T cells are indicated in the right panel ( n = 5/group). C Protein expression of the specific transcription factors T-bet, GATA-3, ROR-γt and Foxp3 for Th1, Th2, Th17 and Treg cells, respectively, in ankle joints. Representative Western blotting bands are exhibited in the left panel and densitometric data normalized to β-actin are indicated in the right panel ( n = 5/group). D-G Contents of the cytokines IFN-γ, IL-4, IL-17A and TGF-β1 in ankle joints measured by ELISA. n = 6/group. ** P < 0.01, versus control or WT mice; # P < 0.05, ## P < 0.01, versus WT CIA mice
Techniques Used: Expressing, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay
d1r (Millipore)
Structured Review

D1r, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Intrarenal Dopaminergic System Is Dysregulated in SS- Resp18 mutant Rats"
Article Title: Intrarenal Dopaminergic System Is Dysregulated in SS- Resp18 mutant Rats
Journal: Biomedicines
doi: 10.3390/biomedicines11010111

Figure Legend Snippet: SS- Resp18 mutant rats have decreased renal D1-like receptor protein expression: SS and SS- Resp18 mutant rats were maintained on a high-salt diet for six weeks, and then the rat kidneys were harvested. Kidney protein lysates were immunoblotted for ( A ) D1R and D5R protein in SS and SS- Resp18 mutant rats, and ( B , C ) respective expressions were quantified by densitometry ( n = 6). Data are mean ± SEM. p = 0.011, p = 0.0003, vs. SS- Resp18 mutant rats, t -test.
Techniques Used: Mutagenesis, Expressing
anti d1 receptor (Millipore)
Structured Review

Anti D1 Receptor, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti d1 receptor/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dopamine receptor D2, but not D1, mediates the reward circuit from the ventral tegmental area to the central amygdala, which is involved in pain relief"
Article Title: Dopamine receptor D2, but not D1, mediates the reward circuit from the ventral tegmental area to the central amygdala, which is involved in pain relief
Journal: Molecular Pain
doi: 10.1177/17448069221145096

Figure Legend Snippet: Pain relief increased dopaminergic D2 receptor expression in injured mice. D2 receptor expression in the CeA was significantly decreased in incised mice treated with PNB compared with incised mice treated with saline (n = 6; * p < 0.05 vs the incision+saline group; one-way ANOVA,Interaction::F (3, 20) = 6.274). The expression of D1 receptor was not significantly changed. (b) In the CeA, D1 receptor was mainly expressed in glutamatergic excitatory neurons (positive for glutaminase). In the CeA, D2 receptor was predominantly expressed in GABAergic inhibitory neurons (positive for GABA). Scale bar, 50 μm.
Techniques Used: Expressing

Figure Legend Snippet:
Techniques Used:
antibodies against d1r (Millipore)
Structured Review

Antibodies Against D1r, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against d1r/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dopamine receptor D2, but not D1, mediates the reward circuit from the ventral tegmental area to the central amygdala, which is involved in pain relief"
Article Title: Dopamine receptor D2, but not D1, mediates the reward circuit from the ventral tegmental area to the central amygdala, which is involved in pain relief
Journal: Molecular Pain
doi: 10.1177/17448069221145096

Figure Legend Snippet: Pain relief increased dopaminergic D2 receptor expression in injured mice. D2 receptor expression in the CeA was significantly decreased in incised mice treated with PNB compared with incised mice treated with saline (n = 6; * p < 0.05 vs the incision+saline group; one-way ANOVA,Interaction::F (3, 20) = 6.274). The expression of D1 receptor was not significantly changed. (b) In the CeA, D1 receptor was mainly expressed in glutamatergic excitatory neurons (positive for glutaminase). In the CeA, D2 receptor was predominantly expressed in GABAergic inhibitory neurons (positive for GABA). Scale bar, 50 μm.
Techniques Used: Expressing

Figure Legend Snippet:
Techniques Used:
rabbit antibody against d1r (Millipore)
Structured Review

Rabbit Antibody Against D1r, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antibody against d1r/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Targeting the D1-N-methyl- d -aspartate receptor complex reduces l -dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson’s rats"
Article Title: Targeting the D1-N-methyl- d -aspartate receptor complex reduces l -dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson’s rats
Journal: Drug Design, Development and Therapy
doi: 10.2147/DDDT.S93487

Figure Legend Snippet: Effects of intrastriatal administration of Tat-fusion interfering peptide (Tat-D1Ri) on D1R–GluN1 interactions. Notes: Tat-D1Ri, Tat-D1Rc, or saline were locally injected into the striatum of the normal adult rat at a rate of 0.2 µL/min. Rat striatal tissues were then used for coimmunoprecipitation experiments to validate the efficacy and selectivity of interfering peptide. The intrastriatal injection of Tat-D1Ri rather than Tat-D1Rc caused reduction of D1R–GluN1 interactions, which demonstrated the effectiveness of Tat-D1Ri. Abbreviations: Tat-D1Rc, Tat-fusion control peptide; Ig, Immunoglobulin; IP, immunoprecipitation; IB, immunoblot.
Techniques Used: Injection, Immunoprecipitation, Western Blot

Figure Legend Snippet: Effects of intrastriatal administration of Tat-D1Ri on membrane D1R subunit expression. Notes: Protein levels were evaluated by Western blotting of proteins extracted from the lesioned striatum of the rat brains. They were assessed in extracts from 6-OHDA-lesioned rats treated with pulsatile l -dopa plus intrastriatal administration of Tat-D1Rc or Tat-D1Ri. ( A ) Representative Western blot analysis of D1R in the membrane fraction; ( B ) densitometric analysis of two blots with specific protein signals normalized to the corresponding GAPDH levels. Data are expressed as means ± SEM. Statistical analysis was conducted by independent-samples t -test. * P =0.05 versus LID + Tat-D1Rc. Abbreviations: 6-OHDA, 6-hydroxydopamine; l -dopa, L-3,4-dihydroxyphenylalanine; LID, l -dopa-induced dyskinesia; SEM, standard error of mean; Tat-D1Ri, Tat-fusion interfering peptide; Tat-D1Rc, Tat-fusion control peptide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Techniques Used: Expressing, Western Blot
rabbit primary antibody against d1r (Millipore)
Structured Review

Rabbit Primary Antibody Against D1r, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit primary antibody against d1r/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Targeting the D1-N-methyl- d -aspartate receptor complex reduces l -dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson’s rats"
Article Title: Targeting the D1-N-methyl- d -aspartate receptor complex reduces l -dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson’s rats
Journal: Drug Design, Development and Therapy
doi: 10.2147/DDDT.S93487

Figure Legend Snippet: Effects of intrastriatal administration of Tat-fusion interfering peptide (Tat-D1Ri) on D1R–GluN1 interactions. Notes: Tat-D1Ri, Tat-D1Rc, or saline were locally injected into the striatum of the normal adult rat at a rate of 0.2 µL/min. Rat striatal tissues were then used for coimmunoprecipitation experiments to validate the efficacy and selectivity of interfering peptide. The intrastriatal injection of Tat-D1Ri rather than Tat-D1Rc caused reduction of D1R–GluN1 interactions, which demonstrated the effectiveness of Tat-D1Ri. Abbreviations: Tat-D1Rc, Tat-fusion control peptide; Ig, Immunoglobulin; IP, immunoprecipitation; IB, immunoblot.
Techniques Used: Injection, Immunoprecipitation, Western Blot

Figure Legend Snippet: Effects of intrastriatal administration of Tat-D1Ri on membrane D1R subunit expression. Notes: Protein levels were evaluated by Western blotting of proteins extracted from the lesioned striatum of the rat brains. They were assessed in extracts from 6-OHDA-lesioned rats treated with pulsatile l -dopa plus intrastriatal administration of Tat-D1Rc or Tat-D1Ri. ( A ) Representative Western blot analysis of D1R in the membrane fraction; ( B ) densitometric analysis of two blots with specific protein signals normalized to the corresponding GAPDH levels. Data are expressed as means ± SEM. Statistical analysis was conducted by independent-samples t -test. * P =0.05 versus LID + Tat-D1Rc. Abbreviations: 6-OHDA, 6-hydroxydopamine; l -dopa, L-3,4-dihydroxyphenylalanine; LID, l -dopa-induced dyskinesia; SEM, standard error of mean; Tat-D1Ri, Tat-fusion interfering peptide; Tat-D1Rc, Tat-fusion control peptide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Techniques Used: Expressing, Western Blot
d1r (Millipore)
Structured Review

D1r, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Targeting the D1-N-methyl- d -aspartate receptor complex reduces l -dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson’s rats"
Article Title: Targeting the D1-N-methyl- d -aspartate receptor complex reduces l -dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson’s rats
Journal: Drug Design, Development and Therapy
doi: 10.2147/DDDT.S93487

Figure Legend Snippet: Effects of intrastriatal administration of Tat-fusion interfering peptide (Tat-D1Ri) on D1R–GluN1 interactions. Notes: Tat-D1Ri, Tat-D1Rc, or saline were locally injected into the striatum of the normal adult rat at a rate of 0.2 µL/min. Rat striatal tissues were then used for coimmunoprecipitation experiments to validate the efficacy and selectivity of interfering peptide. The intrastriatal injection of Tat-D1Ri rather than Tat-D1Rc caused reduction of D1R–GluN1 interactions, which demonstrated the effectiveness of Tat-D1Ri. Abbreviations: Tat-D1Rc, Tat-fusion control peptide; Ig, Immunoglobulin; IP, immunoprecipitation; IB, immunoblot.
Techniques Used: Injection, Immunoprecipitation, Western Blot

Figure Legend Snippet: Effects of intrastriatal administration of Tat-D1Ri on membrane D1R subunit expression. Notes: Protein levels were evaluated by Western blotting of proteins extracted from the lesioned striatum of the rat brains. They were assessed in extracts from 6-OHDA-lesioned rats treated with pulsatile l -dopa plus intrastriatal administration of Tat-D1Rc or Tat-D1Ri. ( A ) Representative Western blot analysis of D1R in the membrane fraction; ( B ) densitometric analysis of two blots with specific protein signals normalized to the corresponding GAPDH levels. Data are expressed as means ± SEM. Statistical analysis was conducted by independent-samples t -test. * P =0.05 versus LID + Tat-D1Rc. Abbreviations: 6-OHDA, 6-hydroxydopamine; l -dopa, L-3,4-dihydroxyphenylalanine; LID, l -dopa-induced dyskinesia; SEM, standard error of mean; Tat-D1Ri, Tat-fusion interfering peptide; Tat-D1Rc, Tat-fusion control peptide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Techniques Used: Expressing, Western Blot
d1 receptor (Millipore)
Structured Review

D1 Receptor, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1 receptor/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Group I mGluR antagonist rescues the deficit of D1-induced LTP in a mouse model of fragile X syndrome"
Article Title: Group I mGluR antagonist rescues the deficit of D1-induced LTP in a mouse model of fragile X syndrome
Journal: Molecular Neurodegeneration
doi: 10.1186/1750-1326-7-24

Figure Legend Snippet: Rescue of D1-induced LTP by DL-AP3 in the Fmr1 KO mice. (A) The pairing training produced a significant, long-lasting potentiation of synaptic responses in Fmr1 WT mice (n = 12 slices/5 mice), but not in Fmr1 KO mice (n = 9 slices/4 mice); (B) DL-AP3 (10 μM) did not alter the amplitude of LTP in Fmr1 WT mice (n = 9 slices/3 mice). DL-AP3 (10 μM) failed to induce LTP in Fmr1 KO mice (n = 11 slices/5 mice). (C) SKF81297 (5μM) facilitated LTP induction in Fmr1 WT mice (n = 8 slices/3 mice), but failed to induce LTP in Fmr1 KO mice (n = 11 slices/5 mice). (D) Bath application of SKF81297 (5 μM) and DL-AP3 (10 μM) induced LTP in Fmr1 WT mice (n = 10 slices/3 mice) (n = 10 slices/3 mice) and markedly rescued the LTP induction by SKF81297 in the Fmr1 KO mice (n = 12 slices/4 mice). (E) SCH23390 (10 μM) blocked the LTP by synergistic application of SKF81297 and DL-AP3 in the KO mice (n = 13 slices/4 mice). (F and G) SKF81297 (5 μM, 30 min) or DL-AP3 (10 μM, 30 min) had no effect on basal synaptic responses without pairing training (n = 8) in the Fmr1 WT and KO mice. (H) Summary of the effects of DL-AP3 or/and SKF81297 on the LTP induction. * p < 0.05, ** p < 0.01 compared with WT; # p < 0.05 compared with control; && p < 0.01 compared with SKF81297 + DL-AP3 in WT mice; @ p < 0.05 compared with SKF81297 + DL-AP3 in KO mice.
Techniques Used: Produced

Figure Legend Snippet: Expression of GRK2 and D1 receptor phosphorylation. Expression levels of GRK2 were decreased in cytosol preparation (A) and increased in membrane preparation (B) from the cultures of Fmr1 KO mice as compared with KO neurons. Synergistic addition of DL-AP3 (10 μM) and SKF81297 (5 μM) reversed redistribution of GRK2 between membrane and cytosol in Fmr1 KO neurons. (C) Synergistic addition of DL-AP3 (10 μM) and SKF81297 (5 μM) reduced the increase of D1 receptor phosphorylation at serine sites to a level similar to WT neurons. n = 4 dishes. * p < 0.05, ** p < 0.01 compared between Fmr1 WT and KO neurons; # p < 0.05 compared with KO control.
Techniques Used: Expressing

Figure Legend Snippet: Expression of D1 receptor, mGluR5, and glutamate receptor subunits. (A) Representatives of western blot from the prefrontal cortex of Fmr1 WT and KO mice. (B) There were no differences in expressions of D1 receptor, mGluR5, NR2A, and NR2B between the WT and Fmr1 KO mice ACC. n = 4 mice per group. (C) Representatives of western blot to detect the level of phosphorylation of NR2B subunit at Tyr-1472 (p-NR2B-Tyr1472). (D) Level of p-NR2B-Tyr1472 was increased after treatment with SKF81297 (5 μM) alone or combining with DL-AP3 (10 μM) in neurons from Fmr1 WT and KO mice. n = 4 dishes. * p < 0.05, ** p < 0.01 compared with control; # p < 0.05 compared between Fmr1 WT and KO mice.
Techniques Used: Expressing, Western Blot
mouse monoclonal anti d1r (Millipore)
Structured Review

Mouse Monoclonal Anti D1r, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti d1r/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dopamine D2 Receptor Stimulation Potentiates PolyQ-Huntingtin-Induced Mouse Striatal Neuron Dysfunctions via Rho/ROCK-II Activation"
Article Title: Dopamine D2 Receptor Stimulation Potentiates PolyQ-Huntingtin-Induced Mouse Striatal Neuron Dysfunctions via Rho/ROCK-II Activation
Journal: PLoS ONE
doi: 10.1371/journal.pone.0008287

Figure Legend Snippet: A–D: primary striatal neurons were transfected or not (Control) with Cont siRNA, D1R siRNA and D2R siRNA. After 72 hours, mRNAs were extracted followed by RT-PCR (A and B) and protein expression levels detected by immunoblot (C and D). A: RT-PCR of D1R gene and, B: RT-PCR D2R. Note the presence of both D2R long and short isoforms. For A and B: ARP gene was used as an housekeeping gene. B and D: immunoblot using specific anti-D1R (C), and specific D2R (D) antibodies. β-tubulin was used for control loading.
Techniques Used: Transfection, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot