cytochrome c reaction buffer  (Millipore)

 
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  • 92
    Name:
    Cytochrome f from spinach
    Description:

    Catalog Number:
    C2285
    Price:
    None
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    Structured Review

    Millipore cytochrome c reaction buffer
    LC-MS/MS spectra of the NAC-BQ modified <t>cytochrome</t> c peptide. The LC-MS/MS raw data was analyzed by Sequest, X!Tandem, and P-Mod, followed by manual validation. The peptide 39 KTGCQAPGFTYTDANK 53 was identified with a 268-Da adduct on K 39 .

    https://www.bioz.com/result/cytochrome c reaction buffer/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytochrome c reaction buffer - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "Utilization of LC-MS/MS Analyses to Identify Site-Specific Chemical Protein Adducts In Vitro"

    Article Title: Utilization of LC-MS/MS Analyses to Identify Site-Specific Chemical Protein Adducts In Vitro

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-60761-849-2_19

    LC-MS/MS spectra of the NAC-BQ modified cytochrome c peptide. The LC-MS/MS raw data was analyzed by Sequest, X!Tandem, and P-Mod, followed by manual validation. The peptide 39 KTGCQAPGFTYTDANK 53 was identified with a 268-Da adduct on K 39 .
    Figure Legend Snippet: LC-MS/MS spectra of the NAC-BQ modified cytochrome c peptide. The LC-MS/MS raw data was analyzed by Sequest, X!Tandem, and P-Mod, followed by manual validation. The peptide 39 KTGCQAPGFTYTDANK 53 was identified with a 268-Da adduct on K 39 .

    Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Modification

    2) Product Images from "Utilization of MALDI-TOF to Determine Chemical-Protein Adduct Formation In Vitro"

    Article Title: Utilization of MALDI-TOF to Determine Chemical-Protein Adduct Formation In Vitro

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-60761-849-2_18

    MALDI-TOF whole protein spectra for cytochrome c reacted with NAC-BQ. ( a ) Control spectrum of cytochrome c in 10 mM Tris–HCl pH 7.5, where the peak at 12,360 m/z indicates native cytochrome c . ( b ) Cytochrome c was incubated in 10 mM Tris–HCl
    Figure Legend Snippet: MALDI-TOF whole protein spectra for cytochrome c reacted with NAC-BQ. ( a ) Control spectrum of cytochrome c in 10 mM Tris–HCl pH 7.5, where the peak at 12,360 m/z indicates native cytochrome c . ( b ) Cytochrome c was incubated in 10 mM Tris–HCl

    Techniques Used: Incubation

    Related Articles

    Staining:

    Article Title: A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
    Article Snippet: .. For cytochrome oxidase staining , sections were incubated at room temperature for 24 hours in 10% sucrose, 0.3 g/L cytochrome C from equine heart (Sigma), 0.02 g/L catalase from bovine liver (Sigma-Aldrich, Saint Louis, MO) and 0.25 g/L DAB (Sigma-Aldrich). .. The endogenous fluorescence of the GFP in Krox20:Cre;TauGFP was not affected after treatment and could be imaged on the same sections; however, the GFP signal was further enhanced by immunostaining.

    Article Title: Repetitive transcranial magnetic stimulation recovers cortical map plasticity induced by sensory deprivation due to deafferentiation
    Article Snippet: .. Barrels within layer 4 were visualized via cytochrome oxydase staining according to Wong‐Riley , using cytochrome C (C‐2506; Sigma) and DAB for visualization. ..

    Article Title: Increased capillaries in mitochondrial myopathy: implications for the regulation of oxygen delivery
    Article Snippet: A sample of muscle (∼100 mg) was immediately transversely oriented and frozen in isopentane cooled by liquid nitrogen for histological and immunohistochemical analysis. .. Serial transverse sections (6–10 µm each) were stained sequentially for: (i) fibre type using the alkaline (pH 10.5) ATPase to distinguish Type 1 and 2 fibres; (ii) respiration-incompetent muscle fibres using either the cytochrome oxidase/succinic dehydrogenase sequential assay ( ) to identify cytochrome c oxidase-deficient fibres or modified Gomori trichrome staining to detect ragged-red fibres in patients with mutations that preserve cytochrome oxidase expression (one patient with a cytochrome b mutation and one with an ND4 mutation); (iii) capillaries using a monoclonal antibody to the capillary endothelium marker CD31 (Chemicon; 1:100 dilution); and (iv) VEGF using a mouse anti-VEGF monoclonal antibody (Chemicon; 1:200 dilution). .. For each biopsy, serial sections were examined in three random and non-overlapping fields and photographed under light microscopy (Nikon Microphot and DXM1200F camera) using Axiovision software.

    Incubation:

    Article Title: A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
    Article Snippet: .. For cytochrome oxidase staining , sections were incubated at room temperature for 24 hours in 10% sucrose, 0.3 g/L cytochrome C from equine heart (Sigma), 0.02 g/L catalase from bovine liver (Sigma-Aldrich, Saint Louis, MO) and 0.25 g/L DAB (Sigma-Aldrich). .. The endogenous fluorescence of the GFP in Krox20:Cre;TauGFP was not affected after treatment and could be imaged on the same sections; however, the GFP signal was further enhanced by immunostaining.

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Forty-eight hours after transfection neurons were treated when appropriate with 30 μM NMDA and fixed. .. For cytochrome c immunofluorescence, neurons were fixed with 4% paraformaldehyde, permeabilized, blocked, and incubated over-night at 4°C with anti-cytochrome c antibody (Millipore). .. Antibody binding was visualized using biotinylated secondary antibody/Cy3-conjugated streptavidin.

    Article Title: Induction of Caspase-3-like activity in Rice following release of cytochrome-f from the chloroplast and subsequent interaction with the Ubiquitin-Proteasome System
    Article Snippet: .. Different concentrations (0.2 or 1 μM) of cytochrome f from spinach (Sigma-Aldrich, USA) was added into the cell-free system, and incubated at 25°C for 5 h. ..

    Immunofluorescence:

    Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death
    Article Snippet: Forty-eight hours after transfection neurons were treated when appropriate with 30 μM NMDA and fixed. .. For cytochrome c immunofluorescence, neurons were fixed with 4% paraformaldehyde, permeabilized, blocked, and incubated over-night at 4°C with anti-cytochrome c antibody (Millipore). .. Antibody binding was visualized using biotinylated secondary antibody/Cy3-conjugated streptavidin.

    Modification:

    Article Title: Increased capillaries in mitochondrial myopathy: implications for the regulation of oxygen delivery
    Article Snippet: A sample of muscle (∼100 mg) was immediately transversely oriented and frozen in isopentane cooled by liquid nitrogen for histological and immunohistochemical analysis. .. Serial transverse sections (6–10 µm each) were stained sequentially for: (i) fibre type using the alkaline (pH 10.5) ATPase to distinguish Type 1 and 2 fibres; (ii) respiration-incompetent muscle fibres using either the cytochrome oxidase/succinic dehydrogenase sequential assay ( ) to identify cytochrome c oxidase-deficient fibres or modified Gomori trichrome staining to detect ragged-red fibres in patients with mutations that preserve cytochrome oxidase expression (one patient with a cytochrome b mutation and one with an ND4 mutation); (iii) capillaries using a monoclonal antibody to the capillary endothelium marker CD31 (Chemicon; 1:100 dilution); and (iv) VEGF using a mouse anti-VEGF monoclonal antibody (Chemicon; 1:200 dilution). .. For each biopsy, serial sections were examined in three random and non-overlapping fields and photographed under light microscopy (Nikon Microphot and DXM1200F camera) using Axiovision software.

    Expressing:

    Article Title: Increased capillaries in mitochondrial myopathy: implications for the regulation of oxygen delivery
    Article Snippet: A sample of muscle (∼100 mg) was immediately transversely oriented and frozen in isopentane cooled by liquid nitrogen for histological and immunohistochemical analysis. .. Serial transverse sections (6–10 µm each) were stained sequentially for: (i) fibre type using the alkaline (pH 10.5) ATPase to distinguish Type 1 and 2 fibres; (ii) respiration-incompetent muscle fibres using either the cytochrome oxidase/succinic dehydrogenase sequential assay ( ) to identify cytochrome c oxidase-deficient fibres or modified Gomori trichrome staining to detect ragged-red fibres in patients with mutations that preserve cytochrome oxidase expression (one patient with a cytochrome b mutation and one with an ND4 mutation); (iii) capillaries using a monoclonal antibody to the capillary endothelium marker CD31 (Chemicon; 1:100 dilution); and (iv) VEGF using a mouse anti-VEGF monoclonal antibody (Chemicon; 1:200 dilution). .. For each biopsy, serial sections were examined in three random and non-overlapping fields and photographed under light microscopy (Nikon Microphot and DXM1200F camera) using Axiovision software.

    Mutagenesis:

    Article Title: Increased capillaries in mitochondrial myopathy: implications for the regulation of oxygen delivery
    Article Snippet: A sample of muscle (∼100 mg) was immediately transversely oriented and frozen in isopentane cooled by liquid nitrogen for histological and immunohistochemical analysis. .. Serial transverse sections (6–10 µm each) were stained sequentially for: (i) fibre type using the alkaline (pH 10.5) ATPase to distinguish Type 1 and 2 fibres; (ii) respiration-incompetent muscle fibres using either the cytochrome oxidase/succinic dehydrogenase sequential assay ( ) to identify cytochrome c oxidase-deficient fibres or modified Gomori trichrome staining to detect ragged-red fibres in patients with mutations that preserve cytochrome oxidase expression (one patient with a cytochrome b mutation and one with an ND4 mutation); (iii) capillaries using a monoclonal antibody to the capillary endothelium marker CD31 (Chemicon; 1:100 dilution); and (iv) VEGF using a mouse anti-VEGF monoclonal antibody (Chemicon; 1:200 dilution). .. For each biopsy, serial sections were examined in three random and non-overlapping fields and photographed under light microscopy (Nikon Microphot and DXM1200F camera) using Axiovision software.

    Marker:

    Article Title: Increased capillaries in mitochondrial myopathy: implications for the regulation of oxygen delivery
    Article Snippet: A sample of muscle (∼100 mg) was immediately transversely oriented and frozen in isopentane cooled by liquid nitrogen for histological and immunohistochemical analysis. .. Serial transverse sections (6–10 µm each) were stained sequentially for: (i) fibre type using the alkaline (pH 10.5) ATPase to distinguish Type 1 and 2 fibres; (ii) respiration-incompetent muscle fibres using either the cytochrome oxidase/succinic dehydrogenase sequential assay ( ) to identify cytochrome c oxidase-deficient fibres or modified Gomori trichrome staining to detect ragged-red fibres in patients with mutations that preserve cytochrome oxidase expression (one patient with a cytochrome b mutation and one with an ND4 mutation); (iii) capillaries using a monoclonal antibody to the capillary endothelium marker CD31 (Chemicon; 1:100 dilution); and (iv) VEGF using a mouse anti-VEGF monoclonal antibody (Chemicon; 1:200 dilution). .. For each biopsy, serial sections were examined in three random and non-overlapping fields and photographed under light microscopy (Nikon Microphot and DXM1200F camera) using Axiovision software.

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  • 95
    Millipore ferrocytochrome c substrate solution
    The effect of ginsenoside Rg1 on Aβ-induced the release of mitochondrial cytochrome c
    Ferrocytochrome C Substrate Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ferrocytochrome c substrate solution/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ferrocytochrome c substrate solution - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    92
    Millipore cytochrome c reaction buffer
    LC-MS/MS spectra of the NAC-BQ modified <t>cytochrome</t> c peptide. The LC-MS/MS raw data was analyzed by Sequest, X!Tandem, and P-Mod, followed by manual validation. The peptide 39 KTGCQAPGFTYTDANK 53 was identified with a 268-Da adduct on K 39 .
    Cytochrome C Reaction Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytochrome c reaction buffer/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytochrome c reaction buffer - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    Image Search Results


    The effect of ginsenoside Rg1 on Aβ-induced the release of mitochondrial cytochrome c

    Journal: Current Alzheimer Research

    Article Title: Ginsenoside Rg1 Attenuates Oligomeric A?1-42-Induced Mitochondrial Dysfunction

    doi:

    Figure Lengend Snippet: The effect of ginsenoside Rg1 on Aβ-induced the release of mitochondrial cytochrome c

    Article Snippet: The reaction was then started by the addition of 50 μl of ferrocytochrome c substrate solution (0.22 mM) (from Sigma); the change in absorbance of cytochrome c at 550 nm was measured using a DU640 spectrophotometer (Beckman company, USA).

    Techniques:

    The effects of geniposide on Aβ 1-42 -induced release of mitochondrial cytochrome c and caspase-3/9 activity. Neurons were cultured in the presence of 5 μM oligomeric Aβ 1–42  and geniposide (2.5 μM, 5 μM, 10 μM) for 24 h, harvested and the levels of specific proteins were assessed by western blot analysis (A). Levels of cytochrome c in the cytosolic fraction (B) or mitochondrial fractions (C), Geniposide attenuated mitochondrial cytochrome c releasing to cytosolic fraction in neurons induced by oligomeric Aβ 1–42  at 5 μM. Caspase-3/9 activity was assessed by microplate fluorometer. The increased activity of caspase-3/9 was attenuated (d, e) by the present of geniposide in a dose-independent manner. NS: non significance. N = 6 per group of cells. Studies were repeated four times and data were expressed as mean ± SEM of percentage of vehicle-treated cells.

    Journal: PLoS ONE

    Article Title: Geniposide Protects Primary Cortical Neurons against Oligomeric Aβ1-42-Induced Neurotoxicity through a Mitochondrial Pathway

    doi: 10.1371/journal.pone.0152551

    Figure Lengend Snippet: The effects of geniposide on Aβ 1-42 -induced release of mitochondrial cytochrome c and caspase-3/9 activity. Neurons were cultured in the presence of 5 μM oligomeric Aβ 1–42 and geniposide (2.5 μM, 5 μM, 10 μM) for 24 h, harvested and the levels of specific proteins were assessed by western blot analysis (A). Levels of cytochrome c in the cytosolic fraction (B) or mitochondrial fractions (C), Geniposide attenuated mitochondrial cytochrome c releasing to cytosolic fraction in neurons induced by oligomeric Aβ 1–42 at 5 μM. Caspase-3/9 activity was assessed by microplate fluorometer. The increased activity of caspase-3/9 was attenuated (d, e) by the present of geniposide in a dose-independent manner. NS: non significance. N = 6 per group of cells. Studies were repeated four times and data were expressed as mean ± SEM of percentage of vehicle-treated cells.

    Article Snippet: The reaction was started by the addition of 50 μl of ferrocytochrome c substrate solution (0.22 mM) (from Sigma).

    Techniques: Activity Assay, Cell Culture, Western Blot

    LC-MS/MS spectra of the NAC-BQ modified cytochrome c peptide. The LC-MS/MS raw data was analyzed by Sequest, X!Tandem, and P-Mod, followed by manual validation. The peptide 39 KTGCQAPGFTYTDANK 53 was identified with a 268-Da adduct on K 39 .

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Utilization of LC-MS/MS Analyses to Identify Site-Specific Chemical Protein Adducts In Vitro

    doi: 10.1007/978-1-60761-849-2_19

    Figure Lengend Snippet: LC-MS/MS spectra of the NAC-BQ modified cytochrome c peptide. The LC-MS/MS raw data was analyzed by Sequest, X!Tandem, and P-Mod, followed by manual validation. The peptide 39 KTGCQAPGFTYTDANK 53 was identified with a 268-Da adduct on K 39 .

    Article Snippet: Cytochrome c reaction buffer: 10 mM Tris–HCl, pH 7.5 used with horse heart cytochrome c (Sigma).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Modification

    MALDI-TOF whole protein spectra for cytochrome c reacted with NAC-BQ. ( a ) Control spectrum of cytochrome c in 10 mM Tris–HCl pH 7.5, where the peak at 12,360 m/z indicates native cytochrome c . ( b ) Cytochrome c was incubated in 10 mM Tris–HCl

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Utilization of MALDI-TOF to Determine Chemical-Protein Adduct Formation In Vitro

    doi: 10.1007/978-1-60761-849-2_18

    Figure Lengend Snippet: MALDI-TOF whole protein spectra for cytochrome c reacted with NAC-BQ. ( a ) Control spectrum of cytochrome c in 10 mM Tris–HCl pH 7.5, where the peak at 12,360 m/z indicates native cytochrome c . ( b ) Cytochrome c was incubated in 10 mM Tris–HCl

    Article Snippet: Cytochrome c reaction buffer: 10 mM Tris–HCl (Sigma), pH 7.5, used with horse heart cytochrome c (Sigma).

    Techniques: Incubation