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  • 94
    Name:
    Cytidine 5 triphosphate disodium salt
    Description:
    Cytidine 5 triphosphate disodium salt is a P2X4 P2X4 purinergic receptor agonist
    Catalog Number:
    SC-217995
    Price:
    None
    Category:
    Chemicals Protein Interacting Inhibitors Activators Substrates Protein Activators P2X4 Activators Cytidine 5 triphosphate disodium salt
    Applications:
    Cytidine 5′-triphosphate disodium salt is a P2X purinergic receptor agonist
    Purity:
    ≥97%
    Molecular Weight:
    527.12
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    Structured Review

    Santa Cruz Biotechnology ctp
    Cytidine 5 triphosphate disodium salt is a P2X4 P2X4 purinergic receptor agonist
    https://www.bioz.com/result/ctp/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ctp - by Bioz Stars, 2021-07
    94/100 stars

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    SDS Page:

    Article Title: Reductive carboxylation supports redox homeostasis during anchorage-independent growth
    Article Snippet: Western blotting Whole cells/spheroids or mitochondrial lysates were prepared in RIPA buffer and quantified using the BCA Protein Assay (Thermo Scientific). .. Proteins were separated on 4–20% SDS-PAGE gels, transferred to PVDF membranes, and probed with antibodies against IDH1 (ab94571), IDH2 (ab55271), IDH3 (ab58641) from Abcam, PDHα (#459400, Theromo), PDHα-pSer293 (AP1062), GAPDH (AB2302) from Millipore, PDK1 (#3820), Hif1α(#3716) from Cell Signaling, CTP (sc-86392), AIF (sc-13116) from Santa Cruz Biotechnology and Actin (A3853, Sigma). .. EF5 staining Day 7 H460 spheroids were cultured under normoxia (21% oxygen) or hypoxia (1% oxygen) for 16 hours.

    Incubation:

    Article Title: Structural visualization of transcription activated by a multidrug-sensing MerR family regulator
    Article Snippet: The mixture was supplemented with 5 mM MgCl2 and 5 mM TPSb+ •Br– (Santa Cruz Biotechnology) and incubated at 23 °C for 3 h. For EcmrR-promoter-RNAP complex with a heteroduplex DNA scaffold, the mixture was either directly loaded onto a Superdex 200 increase 10/300 GL SEC column for EcmrR-RPo complex purification or supplemented with 200 µM adenosine-5′-triphosphate (ATP) and 200 µM GTP, incubated at 37 °C for 10 min then loaded onto a Superdex 200 increase 10/300 GL SEC column for EcmrR-RPitc-3nt complex purification. .. For EcmrR-promoter-RNAP complex with a fully complementary DNA scaffold, the mixture was supplemented with 200 µM GTP, 200 µM ATP, 200 µM cytidine-5′-triphosphate (CTP) and 2 µM uridine-5′-triphosphate (UTP), incubated at 37 °C for 10 min and purified using a Superdex 200 increase 10/300 GL SEC column. .. Cryo-EM sample preparation and data acquisition Purified EcmrR-RPitc-3nt complex (3.5 µl at ~1.2 µM) was applied to freshly glow-discharged Quantifoil 300 mesh R1.2/1.3 copper grids with holey carbon foil (Electron Microscopy Sciences).

    Purification:

    Article Title: Structural visualization of transcription activated by a multidrug-sensing MerR family regulator
    Article Snippet: The mixture was supplemented with 5 mM MgCl2 and 5 mM TPSb+ •Br– (Santa Cruz Biotechnology) and incubated at 23 °C for 3 h. For EcmrR-promoter-RNAP complex with a heteroduplex DNA scaffold, the mixture was either directly loaded onto a Superdex 200 increase 10/300 GL SEC column for EcmrR-RPo complex purification or supplemented with 200 µM adenosine-5′-triphosphate (ATP) and 200 µM GTP, incubated at 37 °C for 10 min then loaded onto a Superdex 200 increase 10/300 GL SEC column for EcmrR-RPitc-3nt complex purification. .. For EcmrR-promoter-RNAP complex with a fully complementary DNA scaffold, the mixture was supplemented with 200 µM GTP, 200 µM ATP, 200 µM cytidine-5′-triphosphate (CTP) and 2 µM uridine-5′-triphosphate (UTP), incubated at 37 °C for 10 min and purified using a Superdex 200 increase 10/300 GL SEC column. .. Cryo-EM sample preparation and data acquisition Purified EcmrR-RPitc-3nt complex (3.5 µl at ~1.2 µM) was applied to freshly glow-discharged Quantifoil 300 mesh R1.2/1.3 copper grids with holey carbon foil (Electron Microscopy Sciences).

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  • 85
    Santa Cruz Biotechnology mouse anti 5 methyl cytidine antibody
    Global DNA methylation of monoparental (AN, PA) and biparental (IVF-Control) sheep blastocysts. ( A ) Immunostaining <t>anti-5-methyl.(</t> B ) Semiquantitative analysis of fluorescence intensity. Different superscripts indicate p
    Mouse Anti 5 Methyl Cytidine Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti 5 methyl cytidine antibody/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti 5 methyl cytidine antibody - by Bioz Stars, 2021-07
    85/100 stars
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    90
    Santa Cruz Biotechnology adenylyl cyclases
    Western analysis of <t>adenylyl</t> cyclases 3 (AC3) and 5/6(AC5/6) in floxed control and CD ET-1 KO IMCD. All results were normalized to β-actin. Upper panel shows representative blot (n = 8 total). Bottom panel shows densitometry analysis. *p
    Adenylyl Cyclases, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adenylyl cyclases/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
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    N/A
    Cytidine 5 diphosphate disodium is used as a substrate of CDP kinase to produce CTP in support ofDNA and RNA biosynthesis and of ribonucleotide reductase to product dCMP
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    Image Search Results


    Global DNA methylation of monoparental (AN, PA) and biparental (IVF-Control) sheep blastocysts. ( A ) Immunostaining anti-5-methyl.( B ) Semiquantitative analysis of fluorescence intensity. Different superscripts indicate p

    Journal: Cellular Reprogramming

    Article Title: Efficient Production and Cellular Characterization of Sheep Androgenetic Embryos

    doi: 10.1089/cell.2011.0021

    Figure Lengend Snippet: Global DNA methylation of monoparental (AN, PA) and biparental (IVF-Control) sheep blastocysts. ( A ) Immunostaining anti-5-methyl.( B ) Semiquantitative analysis of fluorescence intensity. Different superscripts indicate p

    Article Snippet: Embryos were incubated with a mouse anti-5-Methyl Cytidine antibody (Santa Cruz Biotechnology sc-56615, Santa Cruz, CA) at room temperature for 2 h, washed in blocking medium three times and incubated with goat antimouse IgG FITC conjugate antibody (Sigma F9137) at room temperature for 1 h. Mounted specimens were analyzed with an epifluorescence microscope (Nikon).

    Techniques: DNA Methylation Assay, Immunostaining, Fluorescence

    Western analysis of adenylyl cyclases 3 (AC3) and 5/6(AC5/6) in floxed control and CD ET-1 KO IMCD. All results were normalized to β-actin. Upper panel shows representative blot (n = 8 total). Bottom panel shows densitometry analysis. *p

    Journal: BMC Nephrology

    Article Title: Altered collecting duct adenylyl cyclase content in collecting duct endothelin-1 knockout mice

    doi: 10.1186/1471-2369-8-8

    Figure Lengend Snippet: Western analysis of adenylyl cyclases 3 (AC3) and 5/6(AC5/6) in floxed control and CD ET-1 KO IMCD. All results were normalized to β-actin. Upper panel shows representative blot (n = 8 total). Bottom panel shows densitometry analysis. *p

    Article Snippet: All primary antibodies directed against adenylyl cyclases were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Western Blot

    RT-PCR analysis of adenylyl cyclases 3 (AC3) and 6 (AC6) in floxed control and CD ET-1 KO IMCD. All results were normalized to GAPDH. A representative blot is shown (n = 5 total).

    Journal: BMC Nephrology

    Article Title: Altered collecting duct adenylyl cyclase content in collecting duct endothelin-1 knockout mice

    doi: 10.1186/1471-2369-8-8

    Figure Lengend Snippet: RT-PCR analysis of adenylyl cyclases 3 (AC3) and 6 (AC6) in floxed control and CD ET-1 KO IMCD. All results were normalized to GAPDH. A representative blot is shown (n = 5 total).

    Article Snippet: All primary antibodies directed against adenylyl cyclases were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    ROCK1 deletion prevented down-regulation of type V/VI adenylyl cyclases induced by Gαq

    Journal:

    Article Title: Disruption of ROCK1 gene attenuates cardiac dilation and improves contractile function in pathological cardiac hypertrophy

    doi: 10.1016/j.yjmcc.2007.11.018

    Figure Lengend Snippet: ROCK1 deletion prevented down-regulation of type V/VI adenylyl cyclases induced by Gαq

    Article Snippet: Western blot analysis was performed with an antibody against type V/VI adenylyl cyclase because type V or VI specific antibodies are not commercially available.

    Techniques:

    Mathematical model of the GPCR-cAMP signaling pathway. A model of spatial partial differential equations was generated to simulate the temporal and spatial dynamics of GPCR signaling. (A) Schematic illustration of the basic model. The receptor, G-proteins, and adenylyl cyclase are placed on the plasma membrane, whereas ATP, cAMP, and PDE4 are freely diffusing in the cytosol. PKA is cytosolic, but nondiffusing. (B) Model with the addition of an intracellular signaling compartment (ICSC). To simulate GPCR-cAMP signaling from an ICSC, we placed G-proteins and adenylyl cyclase also on an intracellular membrane and simulated the internalization of both GPCR and ligand to this compartment. (C) Results of simulations. A cell was transiently stimulated by application and removal of the ligand from the extracellular compartment. In a first simulation in which signaling from the ICSC was not implemented (no ICSC), the cAMP response was completely reversible. On the contrary, inclusion of the ICSC in the model lead to sustained cAMP production. Also note the different levels and spatial patterns of PKA activation predicted in the presence or absence of the ICSC.

    Journal: PLoS Biology

    Article Title: Persistent cAMP-Signals Triggered by Internalized G-Protein-Coupled Receptors

    doi: 10.1371/journal.pbio.1000172

    Figure Lengend Snippet: Mathematical model of the GPCR-cAMP signaling pathway. A model of spatial partial differential equations was generated to simulate the temporal and spatial dynamics of GPCR signaling. (A) Schematic illustration of the basic model. The receptor, G-proteins, and adenylyl cyclase are placed on the plasma membrane, whereas ATP, cAMP, and PDE4 are freely diffusing in the cytosol. PKA is cytosolic, but nondiffusing. (B) Model with the addition of an intracellular signaling compartment (ICSC). To simulate GPCR-cAMP signaling from an ICSC, we placed G-proteins and adenylyl cyclase also on an intracellular membrane and simulated the internalization of both GPCR and ligand to this compartment. (C) Results of simulations. A cell was transiently stimulated by application and removal of the ligand from the extracellular compartment. In a first simulation in which signaling from the ICSC was not implemented (no ICSC), the cAMP response was completely reversible. On the contrary, inclusion of the ICSC in the model lead to sustained cAMP production. Also note the different levels and spatial patterns of PKA activation predicted in the presence or absence of the ICSC.

    Article Snippet: Rabbit polyclonal antibodies against Gαs , adenylyl cyclase III, and adenylyl cyclase V/VI were from Santa Cruz Biotechnology.

    Techniques: Generated, Activation Assay

    Subcellular localization of Gα s , adenylyl cyclase III, and internalized TSH in primary thyroid cells. Primary mouse thyroid cells were stimulated with 3 µg/ml TSH-Alexa594 for 10 min, followed by immunofluorescence analysis with antibodies against Gα s (A and B) or adenylyl cyclase III (C). Image stacks on the z -axis were acquired with a laser-scanning confocal microscope. Shown are representative frames. The “3D” in the panels refer to 3-D reconstructions of the areas indicated by the white boxes, calculated from the z -stacks. Here, the reconstructions are observed from the top. To view a complete rotation on the x -axis of the 3D reconstructions, see Videos S4 and S5 . (B) Side-view of the z -stack in (A), cut along the white line, showing a Gα s -positive tubule ending in a vesicle positive for both Gα s and TSH-Alexa594. Throughout the figure, yellow in the merged images is indicative of colocalization. Images in (A–C) are representative of 25–30 cells per condition analyzed in at least three independent experiments.

    Journal: PLoS Biology

    Article Title: Persistent cAMP-Signals Triggered by Internalized G-Protein-Coupled Receptors

    doi: 10.1371/journal.pbio.1000172

    Figure Lengend Snippet: Subcellular localization of Gα s , adenylyl cyclase III, and internalized TSH in primary thyroid cells. Primary mouse thyroid cells were stimulated with 3 µg/ml TSH-Alexa594 for 10 min, followed by immunofluorescence analysis with antibodies against Gα s (A and B) or adenylyl cyclase III (C). Image stacks on the z -axis were acquired with a laser-scanning confocal microscope. Shown are representative frames. The “3D” in the panels refer to 3-D reconstructions of the areas indicated by the white boxes, calculated from the z -stacks. Here, the reconstructions are observed from the top. To view a complete rotation on the x -axis of the 3D reconstructions, see Videos S4 and S5 . (B) Side-view of the z -stack in (A), cut along the white line, showing a Gα s -positive tubule ending in a vesicle positive for both Gα s and TSH-Alexa594. Throughout the figure, yellow in the merged images is indicative of colocalization. Images in (A–C) are representative of 25–30 cells per condition analyzed in at least three independent experiments.

    Article Snippet: Rabbit polyclonal antibodies against Gαs , adenylyl cyclase III, and adenylyl cyclase V/VI were from Santa Cruz Biotechnology.

    Techniques: Immunofluorescence, Microscopy

    Cell fractionation experiments. The plasma membrane and the intracellular fractions of FRTL5 cells were obtained by separation with concanavalin A-coated magnetic beads. (A) Western blot analysis of subcellular markers in the obtained fractions. The following markers were used: Na + /K + ATPase for the plasma membrane, the early endosome antigen 1 (EEA1) for early endosomes, and Golgi 58K for the Golgi complex. 1, total homogenate. 2, first eluate from the magnetic beads, corresponding to the plasma membrane fraction. 3, postnuclear supernatant. 4, second eluate from the magnetic beads. 5, intracellular fraction. (B) Western blot for Gα s and adenylyl cyclase III (AC III) in the same fractions as in (A). (C) Effect of TSH stimulation on adenylyl cyclase activity in the subcellular fractions. FRTL5 cells were starved for 24 h in medium without TSH and either stimulated with 30 U/l TSH for 30 min or mock stimulated (control), followed by cell fractionation with concanavalin A-coated magnetic beads. The adenylyl cyclase activity in the plasma membrane and intracellular fractions was then determined in the absence of stimuli (−) or in the presence of either 30 U/l TSH (+TSH) or 10 µM forskolin. The results were normalized for the maximal adenylyl cyclase activity measured in the presence of forskolin. Shown are the data from three independent experiments. Error bars indicate SEM.

    Journal: PLoS Biology

    Article Title: Persistent cAMP-Signals Triggered by Internalized G-Protein-Coupled Receptors

    doi: 10.1371/journal.pbio.1000172

    Figure Lengend Snippet: Cell fractionation experiments. The plasma membrane and the intracellular fractions of FRTL5 cells were obtained by separation with concanavalin A-coated magnetic beads. (A) Western blot analysis of subcellular markers in the obtained fractions. The following markers were used: Na + /K + ATPase for the plasma membrane, the early endosome antigen 1 (EEA1) for early endosomes, and Golgi 58K for the Golgi complex. 1, total homogenate. 2, first eluate from the magnetic beads, corresponding to the plasma membrane fraction. 3, postnuclear supernatant. 4, second eluate from the magnetic beads. 5, intracellular fraction. (B) Western blot for Gα s and adenylyl cyclase III (AC III) in the same fractions as in (A). (C) Effect of TSH stimulation on adenylyl cyclase activity in the subcellular fractions. FRTL5 cells were starved for 24 h in medium without TSH and either stimulated with 30 U/l TSH for 30 min or mock stimulated (control), followed by cell fractionation with concanavalin A-coated magnetic beads. The adenylyl cyclase activity in the plasma membrane and intracellular fractions was then determined in the absence of stimuli (−) or in the presence of either 30 U/l TSH (+TSH) or 10 µM forskolin. The results were normalized for the maximal adenylyl cyclase activity measured in the presence of forskolin. Shown are the data from three independent experiments. Error bars indicate SEM.

    Article Snippet: Rabbit polyclonal antibodies against Gαs , adenylyl cyclase III, and adenylyl cyclase V/VI were from Santa Cruz Biotechnology.

    Techniques: Cell Fractionation, Magnetic Beads, Western Blot, Activity Assay

    BODIPY-forskolin labeling of adenylyl cyclases. (A) Test experiment in HEK293 cells. HEK293 cells were either transfected with canine adenylyl cyclase VI cDNA (AC VI) or mock transfected (M.T.). Forty-eight hours after the transfection, they were stained with BODIPY-forskolin and directly visualized with a fluorescent microscope. Note the higher staining in cells overexpressing adenylyl cyclase VI. (B) BODIPY-forskolin labeling of primary thyroid cells. Mouse primary thyroid cells were stained with BODIPY-forskolin and visualized with a TIRF microscope set to have a high penetration depth. (C) Live-cell imaging of adenylyl cyclases and internalized TSH in primary thyroid cells. Primary mouse thyroid cells were stimulated with 3 µg/ml TSH-Alexa594 for 20 min, followed by 10 min staining with BODIPY-forskolin. TSH-Alexa594 and BODIPY-forskolin were visualized with a TIRF microscope as above. A frequent colocalization between TSH-Alexa594 and BODIPY-forskolin on intracellular vesicles and small tubulovesicular structures was observed. (D) Triple staining for adenylyl cyclases, Gα s , and TSH. Mouse primary thyroid cells were stimulated with 3 µg/ml TSH-Alexa594 for 20 min, fixed, and then processed for Gα s immunofluorescence. Immediately before imaging, the coverslips were mounted in an experimental chamber, stained with BODIPY-forskolin, and directly visualized with a confocal microscope. White is indicative of triple colocalization. Images in (A) are representative of three independent experiments. Images in (B–D) are representative of more than 20 cells per condition analyzed in at least three independent experiments.

    Journal: PLoS Biology

    Article Title: Persistent cAMP-Signals Triggered by Internalized G-Protein-Coupled Receptors

    doi: 10.1371/journal.pbio.1000172

    Figure Lengend Snippet: BODIPY-forskolin labeling of adenylyl cyclases. (A) Test experiment in HEK293 cells. HEK293 cells were either transfected with canine adenylyl cyclase VI cDNA (AC VI) or mock transfected (M.T.). Forty-eight hours after the transfection, they were stained with BODIPY-forskolin and directly visualized with a fluorescent microscope. Note the higher staining in cells overexpressing adenylyl cyclase VI. (B) BODIPY-forskolin labeling of primary thyroid cells. Mouse primary thyroid cells were stained with BODIPY-forskolin and visualized with a TIRF microscope set to have a high penetration depth. (C) Live-cell imaging of adenylyl cyclases and internalized TSH in primary thyroid cells. Primary mouse thyroid cells were stimulated with 3 µg/ml TSH-Alexa594 for 20 min, followed by 10 min staining with BODIPY-forskolin. TSH-Alexa594 and BODIPY-forskolin were visualized with a TIRF microscope as above. A frequent colocalization between TSH-Alexa594 and BODIPY-forskolin on intracellular vesicles and small tubulovesicular structures was observed. (D) Triple staining for adenylyl cyclases, Gα s , and TSH. Mouse primary thyroid cells were stimulated with 3 µg/ml TSH-Alexa594 for 20 min, fixed, and then processed for Gα s immunofluorescence. Immediately before imaging, the coverslips were mounted in an experimental chamber, stained with BODIPY-forskolin, and directly visualized with a confocal microscope. White is indicative of triple colocalization. Images in (A) are representative of three independent experiments. Images in (B–D) are representative of more than 20 cells per condition analyzed in at least three independent experiments.

    Article Snippet: Rabbit polyclonal antibodies against Gαs , adenylyl cyclase III, and adenylyl cyclase V/VI were from Santa Cruz Biotechnology.

    Techniques: Labeling, Transfection, Staining, Microscopy, Live Cell Imaging, Immunofluorescence, Imaging