Structured Review

Abnova cygb
5F 203 confers anticancer activity, induces <t>CYGB</t> and pro-apoptotic protein expression and produces caspase 3 cleavage in vitro. (A) Colony formation analysis in MDA-MB-468 cells untransfected, transfected with empty vector or transfected to overexpress CYGB. Lower panel: quantification of colony numbers. (B) Assessment of migration in untransfected, shGFP and shCYGB MDA-MB-468 cells following 5F 203 treatment. Lower panel: wound closure, normalized to control. (C,D) Determination of CYGB, <t>BAK-1,</t> LTA and GADD45A protein expression as well as caspase 3 cleavage in untransfected, shGFP and shCYGB MDA-MB-468 cells following treatment with 5F 203 (1 μM, 72h). Scale bar = 100 μm. Bars, SEM. Statistically significant where indicated as designated * P
Cygb, supplied by Abnova, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cygb/product/Abnova
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cygb - by Bioz Stars, 2020-08
88/100 stars

Images

1) Product Images from "Putative tumor suppressor cytoglobin promotes aryl hydrocarbon receptor ligand-mediated triple negative breast cancer cell death"

Article Title: Putative tumor suppressor cytoglobin promotes aryl hydrocarbon receptor ligand-mediated triple negative breast cancer cell death

Journal: Journal of cellular biochemistry

doi: 10.1002/jcb.27887

5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vitro. (A) Colony formation analysis in MDA-MB-468 cells untransfected, transfected with empty vector or transfected to overexpress CYGB. Lower panel: quantification of colony numbers. (B) Assessment of migration in untransfected, shGFP and shCYGB MDA-MB-468 cells following 5F 203 treatment. Lower panel: wound closure, normalized to control. (C,D) Determination of CYGB, BAK-1, LTA and GADD45A protein expression as well as caspase 3 cleavage in untransfected, shGFP and shCYGB MDA-MB-468 cells following treatment with 5F 203 (1 μM, 72h). Scale bar = 100 μm. Bars, SEM. Statistically significant where indicated as designated * P
Figure Legend Snippet: 5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vitro. (A) Colony formation analysis in MDA-MB-468 cells untransfected, transfected with empty vector or transfected to overexpress CYGB. Lower panel: quantification of colony numbers. (B) Assessment of migration in untransfected, shGFP and shCYGB MDA-MB-468 cells following 5F 203 treatment. Lower panel: wound closure, normalized to control. (C,D) Determination of CYGB, BAK-1, LTA and GADD45A protein expression as well as caspase 3 cleavage in untransfected, shGFP and shCYGB MDA-MB-468 cells following treatment with 5F 203 (1 μM, 72h). Scale bar = 100 μm. Bars, SEM. Statistically significant where indicated as designated * P

Techniques Used: Activity Assay, Expressing, In Vitro, Multiple Displacement Amplification, Transfection, Plasmid Preparation, Migration

5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vivo. (A,B) Effect of Phortress on tumor growth (as measured in tumor volume, N =11 and tumor mass, N = 9) for 3 cycles of 3 doses every other day (Q2D X 3) followed by a 10 d rest period as determined in Materials and Methods. (C,D) CYGB, BAK-1, and GADD45A protein expression as well as caspase 3 cleavage determination in tumor xenografts. N = 6. Bars, SEM. Statistically significant where indicated as designated * P
Figure Legend Snippet: 5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vivo. (A,B) Effect of Phortress on tumor growth (as measured in tumor volume, N =11 and tumor mass, N = 9) for 3 cycles of 3 doses every other day (Q2D X 3) followed by a 10 d rest period as determined in Materials and Methods. (C,D) CYGB, BAK-1, and GADD45A protein expression as well as caspase 3 cleavage determination in tumor xenografts. N = 6. Bars, SEM. Statistically significant where indicated as designated * P

Techniques Used: Activity Assay, Expressing, In Vivo

2) Product Images from "Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues"

Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues

Journal: Cancer Cell International

doi: 10.1186/1475-2867-10-33

Expression of Ngb and Cygb in human astrocytomas . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. Sections of representative examples of Grades I-IV astrocytoma are shown.
Figure Legend Snippet: Expression of Ngb and Cygb in human astrocytomas . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. Sections of representative examples of Grades I-IV astrocytoma are shown.

Techniques Used: Expressing, Staining

Expression of Ngb, Cygb and CA IX in human normal tissues . Tissue microarrays containing cores obtained from various human normal tissues were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) stomach (fundus); (B) small bowel; (C) gallbladder.
Figure Legend Snippet: Expression of Ngb, Cygb and CA IX in human normal tissues . Tissue microarrays containing cores obtained from various human normal tissues were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) stomach (fundus); (B) small bowel; (C) gallbladder.

Techniques Used: Expressing, Staining

Expression of Ngb, Cygb and CA IX in human tumors . Tissue microarrays containing cores obtained from various human tumors were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) ovarian carcinoma; (B) hepatocellular carcinoma; (C) breast infiltrating duct carcinoma.
Figure Legend Snippet: Expression of Ngb, Cygb and CA IX in human tumors . Tissue microarrays containing cores obtained from various human tumors were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) ovarian carcinoma; (B) hepatocellular carcinoma; (C) breast infiltrating duct carcinoma.

Techniques Used: Expressing, Staining

Expression of Cygb and Ngb in human primary brain tumors . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) oligodendroglioma; (B) ependymoblastoma; (C) ganglioglioma.
Figure Legend Snippet: Expression of Cygb and Ngb in human primary brain tumors . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) oligodendroglioma; (B) ependymoblastoma; (C) ganglioglioma.

Techniques Used: Expressing, Staining

Related Articles

Incubation:

Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues
Article Snippet: .. TMAs were processed for antigen retrieval, incubated with blocking buffer (0.5 M glycine in PBS-0.2% brij35) and then with antibodies to Ngb (1:150, mouse anti-human Ngb monoclonal antibody, BioVendor Laboratory Medicine Inc., Czech Republic), Cygb (1:150, mouse anti-human Cygb monoclonal antibody, Abnova Corporation, Taiwan) or Ca IX (1:600, rabbit anti-human CA IX, Novus Biologicals, Inc., USA). .. The specificity of the primary antibodies was demonstrated by using Cygb or Ngb peptides to block positive immunostaining [Additional file ].

Blocking Assay:

Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues
Article Snippet: .. TMAs were processed for antigen retrieval, incubated with blocking buffer (0.5 M glycine in PBS-0.2% brij35) and then with antibodies to Ngb (1:150, mouse anti-human Ngb monoclonal antibody, BioVendor Laboratory Medicine Inc., Czech Republic), Cygb (1:150, mouse anti-human Cygb monoclonal antibody, Abnova Corporation, Taiwan) or Ca IX (1:600, rabbit anti-human CA IX, Novus Biologicals, Inc., USA). .. The specificity of the primary antibodies was demonstrated by using Cygb or Ngb peptides to block positive immunostaining [Additional file ].

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    Abnova anti cygb
    Expression analysis of LEDGF/p75 and <t>CYGB</t> in docetaxel resistant DU 145 cells. LEDGF/p75 and CYGB transcript and protein overexpression in a docetaxel resistant DU 145 cell line (DU 145-DR) were verified using qPCR ( A ) and immunoblotting ( B ), respectively. Transient knockdown of LEDGF/p75 and its effect on CYGB expression were evaluated in DU145-DR cells by qPCR ( C ) and immunoblotting ( D ). Protein loading was assessed with antibody to <t>β-actin.</t> * P
    Anti Cygb, supplied by Abnova, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cygb/product/Abnova
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cygb - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    cygb  (Abnova)
    88
    Abnova cygb
    5F 203 confers anticancer activity, induces <t>CYGB</t> and pro-apoptotic protein expression and produces caspase 3 cleavage in vitro. (A) Colony formation analysis in MDA-MB-468 cells untransfected, transfected with empty vector or transfected to overexpress CYGB. Lower panel: quantification of colony numbers. (B) Assessment of migration in untransfected, shGFP and shCYGB MDA-MB-468 cells following 5F 203 treatment. Lower panel: wound closure, normalized to control. (C,D) Determination of CYGB, <t>BAK-1,</t> LTA and GADD45A protein expression as well as caspase 3 cleavage in untransfected, shGFP and shCYGB MDA-MB-468 cells following treatment with 5F 203 (1 μM, 72h). Scale bar = 100 μm. Bars, SEM. Statistically significant where indicated as designated * P
    Cygb, supplied by Abnova, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cygb/product/Abnova
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cygb - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Expression analysis of LEDGF/p75 and CYGB in docetaxel resistant DU 145 cells. LEDGF/p75 and CYGB transcript and protein overexpression in a docetaxel resistant DU 145 cell line (DU 145-DR) were verified using qPCR ( A ) and immunoblotting ( B ), respectively. Transient knockdown of LEDGF/p75 and its effect on CYGB expression were evaluated in DU145-DR cells by qPCR ( C ) and immunoblotting ( D ). Protein loading was assessed with antibody to β-actin. * P

    Journal: The Prostate

    Article Title: Pathway Specific Gene Expression Profiling Reveals Oxidative Stress Genes Potentially Regulated by Transcription Co-Activator LEDGF/p75 in Prostate Cancer Cells

    doi: 10.1002/pros.21463

    Figure Lengend Snippet: Expression analysis of LEDGF/p75 and CYGB in docetaxel resistant DU 145 cells. LEDGF/p75 and CYGB transcript and protein overexpression in a docetaxel resistant DU 145 cell line (DU 145-DR) were verified using qPCR ( A ) and immunoblotting ( B ), respectively. Transient knockdown of LEDGF/p75 and its effect on CYGB expression were evaluated in DU145-DR cells by qPCR ( C ) and immunoblotting ( D ). Protein loading was assessed with antibody to β-actin. * P

    Article Snippet: The following antibodies were used: Mouse monoclonals anti-β-actin (Sigma-Aldrich), and anti-CYGB (1:200, Abnova); rabbit polyclonals anti-PRDX1 (1:1,000, Abcam), anti-PRDX2 (1:10,000, Abcam), anti-PRDX3 (1:5,000, Abcam), anti-PRDX4 (1:1,000, Abcam), anti-PRDX5 (1:1,000, Santa Cruz Biotechnology), anti-PRDX6 (1:3,000, Abcam), anti-LEDGF/p75 (1:1,000, Novus Biologicals); and horseradish peroxidase (HRP)-labeled secondary IgG antibodies (Zymed).

    Techniques: Expressing, Over Expression, Real-time Polymerase Chain Reaction

    Analysis of changes in CYGB protein expression in response to knockdown or overexpression of LEDG/p75. Expression of CYGB protein was evaluated by immunoblotting in PC-3 cells with transient knockdown ( A ) and stable overexpression of LEDGF/p75 ( B ), to further validate the qPCR data. Protein loading was assessed with antibody to β-actin.

    Journal: The Prostate

    Article Title: Pathway Specific Gene Expression Profiling Reveals Oxidative Stress Genes Potentially Regulated by Transcription Co-Activator LEDGF/p75 in Prostate Cancer Cells

    doi: 10.1002/pros.21463

    Figure Lengend Snippet: Analysis of changes in CYGB protein expression in response to knockdown or overexpression of LEDG/p75. Expression of CYGB protein was evaluated by immunoblotting in PC-3 cells with transient knockdown ( A ) and stable overexpression of LEDGF/p75 ( B ), to further validate the qPCR data. Protein loading was assessed with antibody to β-actin.

    Article Snippet: The following antibodies were used: Mouse monoclonals anti-β-actin (Sigma-Aldrich), and anti-CYGB (1:200, Abnova); rabbit polyclonals anti-PRDX1 (1:1,000, Abcam), anti-PRDX2 (1:10,000, Abcam), anti-PRDX3 (1:5,000, Abcam), anti-PRDX4 (1:1,000, Abcam), anti-PRDX5 (1:1,000, Santa Cruz Biotechnology), anti-PRDX6 (1:3,000, Abcam), anti-LEDGF/p75 (1:1,000, Novus Biologicals); and horseradish peroxidase (HRP)-labeled secondary IgG antibodies (Zymed).

    Techniques: Expressing, Over Expression, Real-time Polymerase Chain Reaction

    5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vitro. (A) Colony formation analysis in MDA-MB-468 cells untransfected, transfected with empty vector or transfected to overexpress CYGB. Lower panel: quantification of colony numbers. (B) Assessment of migration in untransfected, shGFP and shCYGB MDA-MB-468 cells following 5F 203 treatment. Lower panel: wound closure, normalized to control. (C,D) Determination of CYGB, BAK-1, LTA and GADD45A protein expression as well as caspase 3 cleavage in untransfected, shGFP and shCYGB MDA-MB-468 cells following treatment with 5F 203 (1 μM, 72h). Scale bar = 100 μm. Bars, SEM. Statistically significant where indicated as designated * P

    Journal: Journal of cellular biochemistry

    Article Title: Putative tumor suppressor cytoglobin promotes aryl hydrocarbon receptor ligand-mediated triple negative breast cancer cell death

    doi: 10.1002/jcb.27887

    Figure Lengend Snippet: 5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vitro. (A) Colony formation analysis in MDA-MB-468 cells untransfected, transfected with empty vector or transfected to overexpress CYGB. Lower panel: quantification of colony numbers. (B) Assessment of migration in untransfected, shGFP and shCYGB MDA-MB-468 cells following 5F 203 treatment. Lower panel: wound closure, normalized to control. (C,D) Determination of CYGB, BAK-1, LTA and GADD45A protein expression as well as caspase 3 cleavage in untransfected, shGFP and shCYGB MDA-MB-468 cells following treatment with 5F 203 (1 μM, 72h). Scale bar = 100 μm. Bars, SEM. Statistically significant where indicated as designated * P

    Article Snippet: Antibodies for CYGB (Abnova Corporation Cat# H00114757-A01, RRID:AB_461584), BAK-1 (Cell Signaling Technology Cat# 3814S, RRID:AB_2290287), GADD45a (Cell Signaling Technology Cat# 4632S, RRID:AB_10544538), caspase 3 (Cell Signaling Technology Cat# 9662P, RRID:AB_10839261) and LTA (Abnova Corporation Cat# PAB8441, RRID:AB_1676074) were used. and antibodies for β-actin were obtained from Sigma-Aldrich (St. Louis, MO).

    Techniques: Activity Assay, Expressing, In Vitro, Multiple Displacement Amplification, Transfection, Plasmid Preparation, Migration

    5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vivo. (A,B) Effect of Phortress on tumor growth (as measured in tumor volume, N =11 and tumor mass, N = 9) for 3 cycles of 3 doses every other day (Q2D X 3) followed by a 10 d rest period as determined in Materials and Methods. (C,D) CYGB, BAK-1, and GADD45A protein expression as well as caspase 3 cleavage determination in tumor xenografts. N = 6. Bars, SEM. Statistically significant where indicated as designated * P

    Journal: Journal of cellular biochemistry

    Article Title: Putative tumor suppressor cytoglobin promotes aryl hydrocarbon receptor ligand-mediated triple negative breast cancer cell death

    doi: 10.1002/jcb.27887

    Figure Lengend Snippet: 5F 203 confers anticancer activity, induces CYGB and pro-apoptotic protein expression and produces caspase 3 cleavage in vivo. (A,B) Effect of Phortress on tumor growth (as measured in tumor volume, N =11 and tumor mass, N = 9) for 3 cycles of 3 doses every other day (Q2D X 3) followed by a 10 d rest period as determined in Materials and Methods. (C,D) CYGB, BAK-1, and GADD45A protein expression as well as caspase 3 cleavage determination in tumor xenografts. N = 6. Bars, SEM. Statistically significant where indicated as designated * P

    Article Snippet: Antibodies for CYGB (Abnova Corporation Cat# H00114757-A01, RRID:AB_461584), BAK-1 (Cell Signaling Technology Cat# 3814S, RRID:AB_2290287), GADD45a (Cell Signaling Technology Cat# 4632S, RRID:AB_10544538), caspase 3 (Cell Signaling Technology Cat# 9662P, RRID:AB_10839261) and LTA (Abnova Corporation Cat# PAB8441, RRID:AB_1676074) were used. and antibodies for β-actin were obtained from Sigma-Aldrich (St. Louis, MO).

    Techniques: Activity Assay, Expressing, In Vivo

    Expression of Ngb and Cygb in human astrocytomas . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. Sections of representative examples of Grades I-IV astrocytoma are shown.

    Journal: Cancer Cell International

    Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues

    doi: 10.1186/1475-2867-10-33

    Figure Lengend Snippet: Expression of Ngb and Cygb in human astrocytomas . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. Sections of representative examples of Grades I-IV astrocytoma are shown.

    Article Snippet: TMAs were processed for antigen retrieval, incubated with blocking buffer (0.5 M glycine in PBS-0.2% brij35) and then with antibodies to Ngb (1:150, mouse anti-human Ngb monoclonal antibody, BioVendor Laboratory Medicine Inc., Czech Republic), Cygb (1:150, mouse anti-human Cygb monoclonal antibody, Abnova Corporation, Taiwan) or Ca IX (1:600, rabbit anti-human CA IX, Novus Biologicals, Inc., USA).

    Techniques: Expressing, Staining

    Expression of Ngb, Cygb and CA IX in human normal tissues . Tissue microarrays containing cores obtained from various human normal tissues were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) stomach (fundus); (B) small bowel; (C) gallbladder.

    Journal: Cancer Cell International

    Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues

    doi: 10.1186/1475-2867-10-33

    Figure Lengend Snippet: Expression of Ngb, Cygb and CA IX in human normal tissues . Tissue microarrays containing cores obtained from various human normal tissues were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) stomach (fundus); (B) small bowel; (C) gallbladder.

    Article Snippet: TMAs were processed for antigen retrieval, incubated with blocking buffer (0.5 M glycine in PBS-0.2% brij35) and then with antibodies to Ngb (1:150, mouse anti-human Ngb monoclonal antibody, BioVendor Laboratory Medicine Inc., Czech Republic), Cygb (1:150, mouse anti-human Cygb monoclonal antibody, Abnova Corporation, Taiwan) or Ca IX (1:600, rabbit anti-human CA IX, Novus Biologicals, Inc., USA).

    Techniques: Expressing, Staining

    Expression of Ngb, Cygb and CA IX in human tumors . Tissue microarrays containing cores obtained from various human tumors were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) ovarian carcinoma; (B) hepatocellular carcinoma; (C) breast infiltrating duct carcinoma.

    Journal: Cancer Cell International

    Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues

    doi: 10.1186/1475-2867-10-33

    Figure Lengend Snippet: Expression of Ngb, Cygb and CA IX in human tumors . Tissue microarrays containing cores obtained from various human tumors were stained with to antibodies to Ngb, Cygb or CA IX. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) ovarian carcinoma; (B) hepatocellular carcinoma; (C) breast infiltrating duct carcinoma.

    Article Snippet: TMAs were processed for antigen retrieval, incubated with blocking buffer (0.5 M glycine in PBS-0.2% brij35) and then with antibodies to Ngb (1:150, mouse anti-human Ngb monoclonal antibody, BioVendor Laboratory Medicine Inc., Czech Republic), Cygb (1:150, mouse anti-human Cygb monoclonal antibody, Abnova Corporation, Taiwan) or Ca IX (1:600, rabbit anti-human CA IX, Novus Biologicals, Inc., USA).

    Techniques: Expressing, Staining

    Expression of Cygb and Ngb in human primary brain tumors . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) oligodendroglioma; (B) ependymoblastoma; (C) ganglioglioma.

    Journal: Cancer Cell International

    Article Title: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues

    doi: 10.1186/1475-2867-10-33

    Figure Lengend Snippet: Expression of Cygb and Ngb in human primary brain tumors . Tissue microarrays containing cores obtained from various human brain tumors were stained with to antibodies to Ngb or Cygb. Positive staining was visualized by the chromogenic reaction of HRP with DAB. Photomicrographs were obtained at 20× magnification, and the scale bar indicates 50 μM. (A) oligodendroglioma; (B) ependymoblastoma; (C) ganglioglioma.

    Article Snippet: TMAs were processed for antigen retrieval, incubated with blocking buffer (0.5 M glycine in PBS-0.2% brij35) and then with antibodies to Ngb (1:150, mouse anti-human Ngb monoclonal antibody, BioVendor Laboratory Medicine Inc., Czech Republic), Cygb (1:150, mouse anti-human Cygb monoclonal antibody, Abnova Corporation, Taiwan) or Ca IX (1:600, rabbit anti-human CA IX, Novus Biologicals, Inc., USA).

    Techniques: Expressing, Staining