cygb sirna  (Qiagen)

 
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  • 99
    Name:
    Negative Control siRNA
    Description:
    For negative control of siRNA Kit contents Negative control siRNA 5 nmol
    Catalog Number:
    1022076
    Price:
    170
    Category:
    Negative Control siRNA
    Buy from Supplier


    Structured Review

    Qiagen cygb sirna
    Negative Control siRNA
    For negative control of siRNA Kit contents Negative control siRNA 5 nmol
    https://www.bioz.com/result/cygb sirna/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cygb sirna - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Protection from Intracellular Oxidative Stress by Cytoglobin in Normal and Cancerous Oesophageal Cells"

    Article Title: Protection from Intracellular Oxidative Stress by Cytoglobin in Normal and Cancerous Oesophageal Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0030587

    Quantification of CYGB expression in oesophageal cells subjected to transient transfection or siRNA knockdown. Levels of CYGB expression, compared to ACTB expression, were determined by real time RT-PCR in a parallel sample for each comet assay performed. These expression levels therefore relate directly to the comet assay data presented in Figure 3 . The geometric mean of all experiments is shown for both TE-8 cells (orange bars) and NE1 cells (pink bars).
    Figure Legend Snippet: Quantification of CYGB expression in oesophageal cells subjected to transient transfection or siRNA knockdown. Levels of CYGB expression, compared to ACTB expression, were determined by real time RT-PCR in a parallel sample for each comet assay performed. These expression levels therefore relate directly to the comet assay data presented in Figure 3 . The geometric mean of all experiments is shown for both TE-8 cells (orange bars) and NE1 cells (pink bars).

    Techniques Used: Expressing, Transfection, Quantitative RT-PCR, Single Cell Gel Electrophoresis

    Impact of BSO treatment and CYGB expression on oxidative stress response. Oxidative stress was measured in a: TE-8 and b: NE1 cells. Median DCF fluorescence is the mean of 5 repeats. All values were normalised to the level seen in the control cells with no BSO. Error bars represent SEM.+2020: transfection reagent only; +CYGB: cells transfected with full length CYGB; −ve siRNA: scrambled control; CYGB k/d: CYGB siRNA. * Statistically significant concentration-dependent response (p
    Figure Legend Snippet: Impact of BSO treatment and CYGB expression on oxidative stress response. Oxidative stress was measured in a: TE-8 and b: NE1 cells. Median DCF fluorescence is the mean of 5 repeats. All values were normalised to the level seen in the control cells with no BSO. Error bars represent SEM.+2020: transfection reagent only; +CYGB: cells transfected with full length CYGB; −ve siRNA: scrambled control; CYGB k/d: CYGB siRNA. * Statistically significant concentration-dependent response (p

    Techniques Used: Expressing, Fluorescence, Transfection, Concentration Assay

    Related Articles

    Transfection:

    Article Title: Vasculature Guides Migrating Neuronal Precursors in the Adult Mammalian Forebrain via Brain-Derived Neurotrophic Factor Signaling
    Article Snippet: .. For reconstituted capillaries, siRNA transfection were performed with HiPerFect transfection reagent (Qiagen) according to the instructions of the manufacturer. .. BDNF and control siRNAs were provided from Santa Cruz Biotechnology (sc-42121 and sc-37007, respectively).

    Article Title: Requirement of ATM for Rapid p53 Phosphorylation at Ser46 without Ser/Thr-Gln Sequences ▿
    Article Snippet: .. Each 100 pmol of siRNA was transfected with HiPerfect transfection reagent (Qiagen) and RNAiMax transfection reagent (Invitrogen) into MCF7 or U2OS cells (2 × 105 cells). ..

    Article Title: Diminishment of ?-MSH anti-inflammatory activity in MC1r siRNA-transfected RAW264.7 macrophages
    Article Snippet: .. We optimized the transfection conditions by transfecting the RAW264.7 cells with siRNA targeting the mouse housekeeping GAPDH gene using the Qiagen RNAiFect transfection kit and assessing 24 h later GAPDH gene silencing by quantitative real-time PCR. .. We used endogenous β-actin mRNA levels to normalize the GAPDH mRNA expression, and the remaining GAPDH mRNA was calculated as a percentage of GAPDH mRNA detected in untreated RAW264.7 cells.

    Multiple Displacement Amplification:

    Article Title: Long noncoding RNA MIAT regulates apoptosis and the apoptotic response to chemotherapeutic agents in breast cancer cell lines
    Article Snippet: .. RNA interference by siRNA Cells were nucleofected with Qiagen siRNAs using programmes E-014 for MCF7 and X-013 for MDA-MB-231 and Hs58T, as described previously [ ]. ..

    Negative Control:

    Article Title: Delivery of siRNA Silencing Runx2 Using a Multifunctional Polymer-Lipid Nanoparticle Inhibits Osteogenesis in a Cell Culture Model of Heterotopic Ossification
    Article Snippet: .. Nonspecific control siRNA labeled with fluorescent Cy5 dye at the 3’ end (AllStars Negative Control siRNA) was purchased from Qiagen Sciences (Germantown, MD). ..

    Article Title: Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements
    Article Snippet: .. Cells were incubated at 37°C and 5% CO2 for 48 h, washed with PBS, and infected with trVLPs (TCID50 = 102.76 /0.01 ml) for 48 h. Negative control siRNA (Qiagen; Cat# 1027310) served as a control. .. RNA Extraction and Quantitative Real-Time Reverse Transcription PCR (qRT-PCR) After trVLP infection, 293T cells in 24-well plates were washed with PBS, and total RNA was extracted from cells using TRIzol (Invitrogen; Cat# 15596018) according to the manufacturer’s instructions. qRT-PCR was performed with a EBOV nucleic acid test kit (Zhijiang Bio-tech, Shanghai, China; Cat# QR-0220-02) on an ABI 7500 qPCR system (45°C for 10 min and 95°C for 15 min, followed by 45 cycles at 95°C for 15 s and 60°C for 30 s).

    Article Title: LINE-1 couples EMT programming with acquisition of oncogenic phenotypes in human bronchial epithelial cells
    Article Snippet: .. The scrambled siRNAs used were Silencer® Negative Control #1 siRNA (AM4635), Silencer® Select Negative Control #2 siRNA (4390846), and Qiagen negative control (1022076). .. The siRNAs were transfected using Lipofectamine™ RNAiMAX (Thermo Fisher Scientific), according to the manufacturer’s directions.

    Article Title: CD36 Recruits ?5?1 Integrin to Promote Cytoadherence of P. falciparum-Infected Erythrocytes
    Article Snippet: .. GmBH, Hilden, Germany) and 20 nM siRNA for β1 integrin (Qiagen) or α5 integrin or 20 nM scrambled siRNA (All Stars Negative Control, Qiagen) was added in 100 µl of Optimem. .. Four hours after transfection, 1.0 ml of EBM was added to each dish.

    Infection:

    Article Title: Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements
    Article Snippet: .. Cells were incubated at 37°C and 5% CO2 for 48 h, washed with PBS, and infected with trVLPs (TCID50 = 102.76 /0.01 ml) for 48 h. Negative control siRNA (Qiagen; Cat# 1027310) served as a control. .. RNA Extraction and Quantitative Real-Time Reverse Transcription PCR (qRT-PCR) After trVLP infection, 293T cells in 24-well plates were washed with PBS, and total RNA was extracted from cells using TRIzol (Invitrogen; Cat# 15596018) according to the manufacturer’s instructions. qRT-PCR was performed with a EBOV nucleic acid test kit (Zhijiang Bio-tech, Shanghai, China; Cat# QR-0220-02) on an ABI 7500 qPCR system (45°C for 10 min and 95°C for 15 min, followed by 45 cycles at 95°C for 15 s and 60°C for 30 s).

    Real-time Polymerase Chain Reaction:

    Article Title: Diminishment of ?-MSH anti-inflammatory activity in MC1r siRNA-transfected RAW264.7 macrophages
    Article Snippet: .. We optimized the transfection conditions by transfecting the RAW264.7 cells with siRNA targeting the mouse housekeeping GAPDH gene using the Qiagen RNAiFect transfection kit and assessing 24 h later GAPDH gene silencing by quantitative real-time PCR. .. We used endogenous β-actin mRNA levels to normalize the GAPDH mRNA expression, and the remaining GAPDH mRNA was calculated as a percentage of GAPDH mRNA detected in untreated RAW264.7 cells.

    Incubation:

    Article Title: Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements
    Article Snippet: .. Cells were incubated at 37°C and 5% CO2 for 48 h, washed with PBS, and infected with trVLPs (TCID50 = 102.76 /0.01 ml) for 48 h. Negative control siRNA (Qiagen; Cat# 1027310) served as a control. .. RNA Extraction and Quantitative Real-Time Reverse Transcription PCR (qRT-PCR) After trVLP infection, 293T cells in 24-well plates were washed with PBS, and total RNA was extracted from cells using TRIzol (Invitrogen; Cat# 15596018) according to the manufacturer’s instructions. qRT-PCR was performed with a EBOV nucleic acid test kit (Zhijiang Bio-tech, Shanghai, China; Cat# QR-0220-02) on an ABI 7500 qPCR system (45°C for 10 min and 95°C for 15 min, followed by 45 cycles at 95°C for 15 s and 60°C for 30 s).

    Labeling:

    Article Title: Delivery of siRNA Silencing Runx2 Using a Multifunctional Polymer-Lipid Nanoparticle Inhibits Osteogenesis in a Cell Culture Model of Heterotopic Ossification
    Article Snippet: .. Nonspecific control siRNA labeled with fluorescent Cy5 dye at the 3’ end (AllStars Negative Control siRNA) was purchased from Qiagen Sciences (Germantown, MD). ..

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  • 99
    Qiagen cygb sirna
    Quantification of <t>CYGB</t> expression in oesophageal cells subjected to transient transfection or <t>siRNA</t> knockdown. Levels of CYGB expression, compared to ACTB expression, were determined by real time RT-PCR in a parallel sample for each comet assay performed. These expression levels therefore relate directly to the comet assay data presented in Figure 3 . The geometric mean of all experiments is shown for both TE-8 cells (orange bars) and NE1 cells (pink bars).
    Cygb Sirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cygb sirna/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cygb sirna - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    86
    Qiagen human cygb
    <t>CYGB</t> -knocked down melanoma cells increase proliferation. (A) Immunoblot data for C32TG and G361 cells transfected with CYGB <t>siRNA</t> or control siRNA. β-actin was used as a loading control. (B) Cellular proliferation pattern for G361 and C32TG cells transfected with CYGB siRNA (si_CYGB) and control siRNA (si_Control). The MTT analysis was performed daily (1d to 4d) post-transfection. The value represents the mean from three independent experiments; OD value, 570 nm. bars, SEM. * P
    Human Cygb, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cygb/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human cygb - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    Image Search Results


    Quantification of CYGB expression in oesophageal cells subjected to transient transfection or siRNA knockdown. Levels of CYGB expression, compared to ACTB expression, were determined by real time RT-PCR in a parallel sample for each comet assay performed. These expression levels therefore relate directly to the comet assay data presented in Figure 3 . The geometric mean of all experiments is shown for both TE-8 cells (orange bars) and NE1 cells (pink bars).

    Journal: PLoS ONE

    Article Title: Protection from Intracellular Oxidative Stress by Cytoglobin in Normal and Cancerous Oesophageal Cells

    doi: 10.1371/journal.pone.0030587

    Figure Lengend Snippet: Quantification of CYGB expression in oesophageal cells subjected to transient transfection or siRNA knockdown. Levels of CYGB expression, compared to ACTB expression, were determined by real time RT-PCR in a parallel sample for each comet assay performed. These expression levels therefore relate directly to the comet assay data presented in Figure 3 . The geometric mean of all experiments is shown for both TE-8 cells (orange bars) and NE1 cells (pink bars).

    Article Snippet: Cells were transfected with 25 nM final concentration of either a negative control scrambled siRNA (Ambion; AM4611) or CYGB siRNA (Qiagen; SI03228323 or Ambion; s41571) using 15 µg ml−1 TransIT siQuest reagent (Mirus Bio) according to the manufacturer's instructions.

    Techniques: Expressing, Transfection, Quantitative RT-PCR, Single Cell Gel Electrophoresis

    Impact of BSO treatment and CYGB expression on oxidative stress response. Oxidative stress was measured in a: TE-8 and b: NE1 cells. Median DCF fluorescence is the mean of 5 repeats. All values were normalised to the level seen in the control cells with no BSO. Error bars represent SEM.+2020: transfection reagent only; +CYGB: cells transfected with full length CYGB; −ve siRNA: scrambled control; CYGB k/d: CYGB siRNA. * Statistically significant concentration-dependent response (p

    Journal: PLoS ONE

    Article Title: Protection from Intracellular Oxidative Stress by Cytoglobin in Normal and Cancerous Oesophageal Cells

    doi: 10.1371/journal.pone.0030587

    Figure Lengend Snippet: Impact of BSO treatment and CYGB expression on oxidative stress response. Oxidative stress was measured in a: TE-8 and b: NE1 cells. Median DCF fluorescence is the mean of 5 repeats. All values were normalised to the level seen in the control cells with no BSO. Error bars represent SEM.+2020: transfection reagent only; +CYGB: cells transfected with full length CYGB; −ve siRNA: scrambled control; CYGB k/d: CYGB siRNA. * Statistically significant concentration-dependent response (p

    Article Snippet: Cells were transfected with 25 nM final concentration of either a negative control scrambled siRNA (Ambion; AM4611) or CYGB siRNA (Qiagen; SI03228323 or Ambion; s41571) using 15 µg ml−1 TransIT siQuest reagent (Mirus Bio) according to the manufacturer's instructions.

    Techniques: Expressing, Fluorescence, Transfection, Concentration Assay

    CYGB -knocked down melanoma cells increase proliferation. (A) Immunoblot data for C32TG and G361 cells transfected with CYGB siRNA or control siRNA. β-actin was used as a loading control. (B) Cellular proliferation pattern for G361 and C32TG cells transfected with CYGB siRNA (si_CYGB) and control siRNA (si_Control). The MTT analysis was performed daily (1d to 4d) post-transfection. The value represents the mean from three independent experiments; OD value, 570 nm. bars, SEM. * P

    Journal: PLoS ONE

    Article Title: Melanoma Transition Is Frequently Accompanied by a Loss of Cytoglobin Expression in Melanocytes: A Novel Expression Site of Cytoglobin

    doi: 10.1371/journal.pone.0094772

    Figure Lengend Snippet: CYGB -knocked down melanoma cells increase proliferation. (A) Immunoblot data for C32TG and G361 cells transfected with CYGB siRNA or control siRNA. β-actin was used as a loading control. (B) Cellular proliferation pattern for G361 and C32TG cells transfected with CYGB siRNA (si_CYGB) and control siRNA (si_Control). The MTT analysis was performed daily (1d to 4d) post-transfection. The value represents the mean from three independent experiments; OD value, 570 nm. bars, SEM. * P

    Article Snippet: siRNA Transfection siRNA targeting human CYGB and negative control siRNA were purchased from Qiagen.

    Techniques: Transfection, MTT Assay

    CYGB protects G361 cells from H 2 O 2 -induced cell death. A flow cytometric analysis was performed to determine the ROS level (A) and apoptosis (B) in G361 cells transfected with a CYGB-siRNA or a control-siRNA. In (A) , 2′-, 7′-dichlorofluorescein diacetate (DCFH-DA) was used. CYGB-knocked down G361 cells treated with 3 mM N-acetyl-L-cystein (NAC) were also compared. (B) Annexin V and PI staining was done after exposure of the cells to 100 μM H 2 O 2 for 0 h (−) or 24 h (+). At an early stage of apoptosis the cells bind to Annexin V while still excluding PI. At a late stage of apoptosis they bind to Annexin V and stain brightly with PI. (a) Control-siRNA-transfected, and (b) CYGB-siRNA-transfected G361 cells.

    Journal: PLoS ONE

    Article Title: Melanoma Transition Is Frequently Accompanied by a Loss of Cytoglobin Expression in Melanocytes: A Novel Expression Site of Cytoglobin

    doi: 10.1371/journal.pone.0094772

    Figure Lengend Snippet: CYGB protects G361 cells from H 2 O 2 -induced cell death. A flow cytometric analysis was performed to determine the ROS level (A) and apoptosis (B) in G361 cells transfected with a CYGB-siRNA or a control-siRNA. In (A) , 2′-, 7′-dichlorofluorescein diacetate (DCFH-DA) was used. CYGB-knocked down G361 cells treated with 3 mM N-acetyl-L-cystein (NAC) were also compared. (B) Annexin V and PI staining was done after exposure of the cells to 100 μM H 2 O 2 for 0 h (−) or 24 h (+). At an early stage of apoptosis the cells bind to Annexin V while still excluding PI. At a late stage of apoptosis they bind to Annexin V and stain brightly with PI. (a) Control-siRNA-transfected, and (b) CYGB-siRNA-transfected G361 cells.

    Article Snippet: siRNA Transfection siRNA targeting human CYGB and negative control siRNA were purchased from Qiagen.

    Techniques: Flow Cytometry, Transfection, Staining