cyclin d1 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 antibody
    Cyclin D1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1 antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclin d1 antibody - by Bioz Stars, 2023-06
    86/100 stars

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    cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1
    Effect of 6n on the expression level of select cap-dependent transcripts. MiaPaCa-2 cells were treated for 6 h and protein levels were measured using Western blot. (A) Structure of 6n . (B) Protein levels following treatment with 60 μM compound. (C) Dose-dependent inhibition of ODC1 and <t>cyclin</t> <t>D1</t> expression.
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    cyclin d1 - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Design of Cell-Permeable Inhibitors of Eukaryotic Translation Initiation Factor 4E (eIF4E) for Inhibiting Aberrant Cap-Dependent Translation in Cancer"

    Article Title: Design of Cell-Permeable Inhibitors of Eukaryotic Translation Initiation Factor 4E (eIF4E) for Inhibiting Aberrant Cap-Dependent Translation in Cancer

    Journal: bioRxiv

    doi: 10.1101/2023.05.23.541912

    Effect of 6n on the expression level of select cap-dependent transcripts. MiaPaCa-2 cells were treated for 6 h and protein levels were measured using Western blot. (A) Structure of 6n . (B) Protein levels following treatment with 60 μM compound. (C) Dose-dependent inhibition of ODC1 and cyclin D1 expression.
    Figure Legend Snippet: Effect of 6n on the expression level of select cap-dependent transcripts. MiaPaCa-2 cells were treated for 6 h and protein levels were measured using Western blot. (A) Structure of 6n . (B) Protein levels following treatment with 60 μM compound. (C) Dose-dependent inhibition of ODC1 and cyclin D1 expression.

    Techniques Used: Expressing, Western Blot, Inhibition

    cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1
    Differentiation of dermal fibroblasts into myofibroblasts. DF (100,000 cells/well) were cultured with or without TGFβ (TGFb in the graphs, 10 ng/mL) for 72 h. Then, cells were harvested and counted, proteins extracted, and the levels of <t>cyclin-D1,</t> αSMA, collagen-I, and elastin measured using Western blot. ( A ) describes the average number of cells at the end of the experiment (mean + SD). ( B , D – F ) show graphs of the average levels of cyclin-D1, αSMA, collagen-I, and elastin in cells exposed or not exposed to TGFβ for 72 h. ( C ) shows representative Western blot images of protein levels. * The results are significantly different ( p ≤ 0.05, n = 5–6).
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "αvβ3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis"

    Article Title: αvβ3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms24108927

    Differentiation of dermal fibroblasts into myofibroblasts. DF (100,000 cells/well) were cultured with or without TGFβ (TGFb in the graphs, 10 ng/mL) for 72 h. Then, cells were harvested and counted, proteins extracted, and the levels of cyclin-D1, αSMA, collagen-I, and elastin measured using Western blot. ( A ) describes the average number of cells at the end of the experiment (mean + SD). ( B , D – F ) show graphs of the average levels of cyclin-D1, αSMA, collagen-I, and elastin in cells exposed or not exposed to TGFβ for 72 h. ( C ) shows representative Western blot images of protein levels. * The results are significantly different ( p ≤ 0.05, n = 5–6).
    Figure Legend Snippet: Differentiation of dermal fibroblasts into myofibroblasts. DF (100,000 cells/well) were cultured with or without TGFβ (TGFb in the graphs, 10 ng/mL) for 72 h. Then, cells were harvested and counted, proteins extracted, and the levels of cyclin-D1, αSMA, collagen-I, and elastin measured using Western blot. ( A ) describes the average number of cells at the end of the experiment (mean + SD). ( B , D – F ) show graphs of the average levels of cyclin-D1, αSMA, collagen-I, and elastin in cells exposed or not exposed to TGFβ for 72 h. ( C ) shows representative Western blot images of protein levels. * The results are significantly different ( p ≤ 0.05, n = 5–6).

    Techniques Used: Cell Culture, Western Blot

    The effect of normal or fibrotic-ECM on naive DF: DF (100,000 cells/well) were cultured on normal (−TGFβ) or fibrotic-ECMs (+TGFβ, +−TGFβ) for 45 min or 48–72 h (TGFb in the graphs). Then, one of the following procedures was performed: (1) the secretomes were collected for evaluation of MMPs activity (48 h, ( B )); (2) the cells were harvested and the proteins were extracted from the cells either after 45 min to evaluate the level of pSMAD3 ( C ), or after 48 h to evaluate the levels of αv ( A ), αSMA ( E ), collagen-I ( F ), elastin ( G ), β3 ( H ), and cyclin-D1 ( I ) with Western blot; (3) cells were counted using an automatic cell counter and trypan-blue (72 h, ( J )); or (4) the cells were stained for αvβ3 using ICC ( M ). ( D ) shows representative zymogram gel of MMP9. ( D , K , L ) contain representative Western blots images of αv, β3, pSMAD, and tubulin ( D ), αSMA, collagen-I, elastin, and tubulin ( K ), and cyclin-D1 and tubulin ( L ) in the different treatments. ( M ) shows photomicrographs of DF cultured on normal (−TGFβ) or fibrotic (+TGFβ) stained with anti-αvb3 or isotype-matched control antibodies, (×200). The nucleus was stained with hematoxylin * The results are significantly different ( p < 0.05, n = 5).
    Figure Legend Snippet: The effect of normal or fibrotic-ECM on naive DF: DF (100,000 cells/well) were cultured on normal (−TGFβ) or fibrotic-ECMs (+TGFβ, +−TGFβ) for 45 min or 48–72 h (TGFb in the graphs). Then, one of the following procedures was performed: (1) the secretomes were collected for evaluation of MMPs activity (48 h, ( B )); (2) the cells were harvested and the proteins were extracted from the cells either after 45 min to evaluate the level of pSMAD3 ( C ), or after 48 h to evaluate the levels of αv ( A ), αSMA ( E ), collagen-I ( F ), elastin ( G ), β3 ( H ), and cyclin-D1 ( I ) with Western blot; (3) cells were counted using an automatic cell counter and trypan-blue (72 h, ( J )); or (4) the cells were stained for αvβ3 using ICC ( M ). ( D ) shows representative zymogram gel of MMP9. ( D , K , L ) contain representative Western blots images of αv, β3, pSMAD, and tubulin ( D ), αSMA, collagen-I, elastin, and tubulin ( K ), and cyclin-D1 and tubulin ( L ) in the different treatments. ( M ) shows photomicrographs of DF cultured on normal (−TGFβ) or fibrotic (+TGFβ) stained with anti-αvb3 or isotype-matched control antibodies, (×200). The nucleus was stained with hematoxylin * The results are significantly different ( p < 0.05, n = 5).

    Techniques Used: Cell Culture, Activity Assay, Western Blot, Staining

    The effect of fibrotic-ECM and tetrac on DF cell proliferation and death and on the levels of MF biomarkers, αvβ3, miRNA-2,1 and D3: DF (100,000 cells/well) were cultured on normal (−TGFβ (TGFb in the graphs)) or fibrotic-ECM (+TGFβ) with/without 0.5 μM tetrac or its solvent (DMSO-KOH propylene glycol) for 48 h. Then, cells were harvested, and the following was undertaken: (1) the cells were counted using a counter and trypan-blue; (2) the proteins were extracted from the cells, and protein levels were measured by Western blot; and (3) the RNA was extracted from the cells, and the level of miRNA-21 was evaluated using qPCR. ( A , H ) shows the average number of total cells, live cells, viability, and cyclin-D1 at the end of the experiment (mean + SD). ( B – F , I , J ) show collagen-I ( B ), elastin ( C ), αSMA ( D ), αv ( E ), β3 ( F ), miRNA-21 ( I ), and D3 ( J ) levels, in cells cultured on ECMs with/without tetrac. ( G ) shows a schematic demonstration of the αvβ3 binding site of RGD, T3, T4 and tetrac. ( K ) shows representative Western blot images of protein levels in the different treatments. The blots contain skipping lanes. * The results are significantly different ( p ≤ 0.05, n = 4–6).
    Figure Legend Snippet: The effect of fibrotic-ECM and tetrac on DF cell proliferation and death and on the levels of MF biomarkers, αvβ3, miRNA-2,1 and D3: DF (100,000 cells/well) were cultured on normal (−TGFβ (TGFb in the graphs)) or fibrotic-ECM (+TGFβ) with/without 0.5 μM tetrac or its solvent (DMSO-KOH propylene glycol) for 48 h. Then, cells were harvested, and the following was undertaken: (1) the cells were counted using a counter and trypan-blue; (2) the proteins were extracted from the cells, and protein levels were measured by Western blot; and (3) the RNA was extracted from the cells, and the level of miRNA-21 was evaluated using qPCR. ( A , H ) shows the average number of total cells, live cells, viability, and cyclin-D1 at the end of the experiment (mean + SD). ( B – F , I , J ) show collagen-I ( B ), elastin ( C ), αSMA ( D ), αv ( E ), β3 ( F ), miRNA-21 ( I ), and D3 ( J ) levels, in cells cultured on ECMs with/without tetrac. ( G ) shows a schematic demonstration of the αvβ3 binding site of RGD, T3, T4 and tetrac. ( K ) shows representative Western blot images of protein levels in the different treatments. The blots contain skipping lanes. * The results are significantly different ( p ≤ 0.05, n = 4–6).

    Techniques Used: Cell Culture, Western Blot, Binding Assay

    List of antibodies.
    Figure Legend Snippet: List of antibodies.

    Techniques Used: Hybridization

    cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    86/100 stars

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    cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1
    Compound 25 inhibited IL-6-induced pY705-STAT3 and the expression of downstream proteins. ( A , B ) Hela ( A ) and MDA-MB-231 ( B ) cells were treated with compound 25 at 0, 2.5, 5, 10, 15 μM for 2 h, then stimulated with IL-6 (20 ng/mL) for 20 min. The expression of STAT3 Y705 phosphorylation upon IL-6 stimulation was detected by Western blotting. Results are expressed as mean ± SD. n = 3. * p < 0.05 vs. control. ( C , D ) A549 ( C ) and DU145 ( D ) cells were treated with compound 25 at 0, 1, 2.5, 5, 10, 15 μM for 24 h, and then lysed for Western blot analysis. The expression of the downstream gene proteins c-Myc, <t>Cyclin</t> <t>D1,</t> and Bcl-xL was detected. Results are expressed as mean ± SD. n = 3. * p < 0.05 vs. control.
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
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    1) Product Images from "A Novel Ageladine A Derivative Acts as a STAT3 Inhibitor and Exhibits Potential Antitumor Effects"

    Article Title: A Novel Ageladine A Derivative Acts as a STAT3 Inhibitor and Exhibits Potential Antitumor Effects

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms24108859

    Compound 25 inhibited IL-6-induced pY705-STAT3 and the expression of downstream proteins. ( A , B ) Hela ( A ) and MDA-MB-231 ( B ) cells were treated with compound 25 at 0, 2.5, 5, 10, 15 μM for 2 h, then stimulated with IL-6 (20 ng/mL) for 20 min. The expression of STAT3 Y705 phosphorylation upon IL-6 stimulation was detected by Western blotting. Results are expressed as mean ± SD. n = 3. * p < 0.05 vs. control. ( C , D ) A549 ( C ) and DU145 ( D ) cells were treated with compound 25 at 0, 1, 2.5, 5, 10, 15 μM for 24 h, and then lysed for Western blot analysis. The expression of the downstream gene proteins c-Myc, Cyclin D1, and Bcl-xL was detected. Results are expressed as mean ± SD. n = 3. * p < 0.05 vs. control.
    Figure Legend Snippet: Compound 25 inhibited IL-6-induced pY705-STAT3 and the expression of downstream proteins. ( A , B ) Hela ( A ) and MDA-MB-231 ( B ) cells were treated with compound 25 at 0, 2.5, 5, 10, 15 μM for 2 h, then stimulated with IL-6 (20 ng/mL) for 20 min. The expression of STAT3 Y705 phosphorylation upon IL-6 stimulation was detected by Western blotting. Results are expressed as mean ± SD. n = 3. * p < 0.05 vs. control. ( C , D ) A549 ( C ) and DU145 ( D ) cells were treated with compound 25 at 0, 1, 2.5, 5, 10, 15 μM for 24 h, and then lysed for Western blot analysis. The expression of the downstream gene proteins c-Myc, Cyclin D1, and Bcl-xL was detected. Results are expressed as mean ± SD. n = 3. * p < 0.05 vs. control.

    Techniques Used: Expressing, Western Blot

    cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
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    cyclin d1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
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    anti cyclin d1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti cyclin d1
    Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin d1/product/Cell Signaling Technology Inc
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    cyclin d1  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    Cell Signaling Technology Inc cyclin d1 antibody
    Cyclin D1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc cyclin d1
    Effect of 6n on the expression level of select cap-dependent transcripts. MiaPaCa-2 cells were treated for 6 h and protein levels were measured using Western blot. (A) Structure of 6n . (B) Protein levels following treatment with 60 μM compound. (C) Dose-dependent inhibition of ODC1 and <t>cyclin</t> <t>D1</t> expression.
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
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    anti cyclin d1  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc anti cyclin d1
    Effect of 6n on the expression level of select cap-dependent transcripts. MiaPaCa-2 cells were treated for 6 h and protein levels were measured using Western blot. (A) Structure of 6n . (B) Protein levels following treatment with 60 μM compound. (C) Dose-dependent inhibition of ODC1 and <t>cyclin</t> <t>D1</t> expression.
    Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin d1/product/Cell Signaling Technology Inc
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    Effect of 6n on the expression level of select cap-dependent transcripts. MiaPaCa-2 cells were treated for 6 h and protein levels were measured using Western blot. (A) Structure of 6n . (B) Protein levels following treatment with 60 μM compound. (C) Dose-dependent inhibition of ODC1 and cyclin D1 expression.

    Journal: bioRxiv

    Article Title: Design of Cell-Permeable Inhibitors of Eukaryotic Translation Initiation Factor 4E (eIF4E) for Inhibiting Aberrant Cap-Dependent Translation in Cancer

    doi: 10.1101/2023.05.23.541912

    Figure Lengend Snippet: Effect of 6n on the expression level of select cap-dependent transcripts. MiaPaCa-2 cells were treated for 6 h and protein levels were measured using Western blot. (A) Structure of 6n . (B) Protein levels following treatment with 60 μM compound. (C) Dose-dependent inhibition of ODC1 and cyclin D1 expression.

    Article Snippet: The following antibodies were used in this study: eIF4E (Cell Signaling Technology, 9742), eIF4G (Cell Signaling Technology, 2498), actin-HRP (Santa Cruz Biotechnology, sc-47778), c-Myc (Cell Signaling Technology, 13987), ODC1 (Novus Biologicals, 2878R), and cyclin D1 (Cell Signaling Technology, 2978).

    Techniques: Expressing, Western Blot, Inhibition