cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab
    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab
    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab
    (A) Scheme of sequence motif analysis of AS exons in epidermal differentiation. (B) Representative RBPs and their binding motifs enriched at each cassette exon splicing junction. A–D lettering corresponds to the exon-intron junctions shown in (A). The complete list of predicted RBPs and their binding motifs are in Table S2. (C) RNA immunoprecipitation of candidate RBPs and their predicted RNA targets. SRSF1 is a positive control. (D) Quantitative RT-PCR of RNA immunoprecipitation. HPRT is a negative/specificity control RNA. Data are means ± SEM (n = 3). (E) Quantitative RT-PCR and immunoblot of FUS after treatment of keratinocytes with control (siCTRL) or FUS-targeting siRNAs (siFUS). (F) RT-PCR of alternatively spliced SE events associated with FUS depletion. (G) Number and type of alternative mRNA splicing events associated with FUS depletion. (H) Immunofluorescence of control and FUS-depleted epidermal organotypic tissues. Ki-67 marks proliferating keratinocytes (pink, arrowheads); KRT10 is a differentiation-associated protein (orange/red). White asterisks indicate non-specific antibody staining of cornified layer. Scale bars (gray): 50 μm. (I) Quantitative RT-PCR of progenitor-associated (CCNB1, BNC1, <t>CCND1)</t> and differentiation-associated (KRT1, KRT10, FLG) transcripts in epidermal organotypic tissue. Data are means ± SEM (n = 2).
    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Alternative mRNA splicing events and regulators in epidermal differentiation"

    Article Title: Alternative mRNA splicing events and regulators in epidermal differentiation

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.113814

    (A) Scheme of sequence motif analysis of AS exons in epidermal differentiation. (B) Representative RBPs and their binding motifs enriched at each cassette exon splicing junction. A–D lettering corresponds to the exon-intron junctions shown in (A). The complete list of predicted RBPs and their binding motifs are in Table S2. (C) RNA immunoprecipitation of candidate RBPs and their predicted RNA targets. SRSF1 is a positive control. (D) Quantitative RT-PCR of RNA immunoprecipitation. HPRT is a negative/specificity control RNA. Data are means ± SEM (n = 3). (E) Quantitative RT-PCR and immunoblot of FUS after treatment of keratinocytes with control (siCTRL) or FUS-targeting siRNAs (siFUS). (F) RT-PCR of alternatively spliced SE events associated with FUS depletion. (G) Number and type of alternative mRNA splicing events associated with FUS depletion. (H) Immunofluorescence of control and FUS-depleted epidermal organotypic tissues. Ki-67 marks proliferating keratinocytes (pink, arrowheads); KRT10 is a differentiation-associated protein (orange/red). White asterisks indicate non-specific antibody staining of cornified layer. Scale bars (gray): 50 μm. (I) Quantitative RT-PCR of progenitor-associated (CCNB1, BNC1, CCND1) and differentiation-associated (KRT1, KRT10, FLG) transcripts in epidermal organotypic tissue. Data are means ± SEM (n = 2).
    Figure Legend Snippet: (A) Scheme of sequence motif analysis of AS exons in epidermal differentiation. (B) Representative RBPs and their binding motifs enriched at each cassette exon splicing junction. A–D lettering corresponds to the exon-intron junctions shown in (A). The complete list of predicted RBPs and their binding motifs are in Table S2. (C) RNA immunoprecipitation of candidate RBPs and their predicted RNA targets. SRSF1 is a positive control. (D) Quantitative RT-PCR of RNA immunoprecipitation. HPRT is a negative/specificity control RNA. Data are means ± SEM (n = 3). (E) Quantitative RT-PCR and immunoblot of FUS after treatment of keratinocytes with control (siCTRL) or FUS-targeting siRNAs (siFUS). (F) RT-PCR of alternatively spliced SE events associated with FUS depletion. (G) Number and type of alternative mRNA splicing events associated with FUS depletion. (H) Immunofluorescence of control and FUS-depleted epidermal organotypic tissues. Ki-67 marks proliferating keratinocytes (pink, arrowheads); KRT10 is a differentiation-associated protein (orange/red). White asterisks indicate non-specific antibody staining of cornified layer. Scale bars (gray): 50 μm. (I) Quantitative RT-PCR of progenitor-associated (CCNB1, BNC1, CCND1) and differentiation-associated (KRT1, KRT10, FLG) transcripts in epidermal organotypic tissue. Data are means ± SEM (n = 2).

    Techniques Used: Sequencing, Binding Assay, RNA Immunoprecipitation, Positive Control, Quantitative RT-PCR, Control, Western Blot, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining

    (A) Sashimi plot showing enriched relative expression of exon-12-containing isoforms of MAP3K7 upon epidermal differentiation. (B) Scheme of RT-PCR primers (red triangles) to discern MAP3K7 isoforms containing (MAP3K7-long) or excluding exon 12 (MAP3K7-short). Purple bars represent isoform-targeting siRNAs for short and long MAP3K7 transcripts. Bottom, RT-PCR of in vitro progenitor (P) and differentiated (D) keratinocytes and in vivo laser capture microdissected skin tissue of basal (Bs) progenitor and suprabasal (Sb) differentiated layers. CCND1 and LOR are control transcripts enriched in progenitor and differentiated states, respectively. (C) RT-PCR evaluating MAP3K7 isoform expression after transfection of isoform-targeting siRNAs against short and long isoforms. RPL32 is an invariant expression control. (D) Immunoblot for proteins in the NF-κB signaling pathway in progenitor keratinocytes after treatment with control or MAP3K7-isoform-targeted siRNAs, in the presence or absence of tumor necrosis factor α (TNF-α; 10 ng/mL), to stimulate NF-κB signaling. TNF-α was applied for 30 min, and protein lysates were harvested 48 h later. (E) Intensity quantitation of phospho-p65/RelA from immunoblot experiments. Phosphorylated p65 was normalized internally to total p65, and the unstimulated siCTRL signal was set to 1 for each biological replicate. Data are means ± SEM (n = 3) (one-way ANOVA with a Tukey’s honestly significant difference [HSD] post hoc test), *p < 0.05. (F) RT-PCR and immunoblot for TAK1, the protein product of MAP3K7, after overexpression (OE) of each isoform. (G) ELISA for phosphorylated p65 in keratinocytes after OE of empty vector or MAP3K7 short/long isoforms. Data are means ± SEM (n = 3) (one-way ANOVA with a Tukey’s HSD post hoc test), *p < 0.05.
    Figure Legend Snippet: (A) Sashimi plot showing enriched relative expression of exon-12-containing isoforms of MAP3K7 upon epidermal differentiation. (B) Scheme of RT-PCR primers (red triangles) to discern MAP3K7 isoforms containing (MAP3K7-long) or excluding exon 12 (MAP3K7-short). Purple bars represent isoform-targeting siRNAs for short and long MAP3K7 transcripts. Bottom, RT-PCR of in vitro progenitor (P) and differentiated (D) keratinocytes and in vivo laser capture microdissected skin tissue of basal (Bs) progenitor and suprabasal (Sb) differentiated layers. CCND1 and LOR are control transcripts enriched in progenitor and differentiated states, respectively. (C) RT-PCR evaluating MAP3K7 isoform expression after transfection of isoform-targeting siRNAs against short and long isoforms. RPL32 is an invariant expression control. (D) Immunoblot for proteins in the NF-κB signaling pathway in progenitor keratinocytes after treatment with control or MAP3K7-isoform-targeted siRNAs, in the presence or absence of tumor necrosis factor α (TNF-α; 10 ng/mL), to stimulate NF-κB signaling. TNF-α was applied for 30 min, and protein lysates were harvested 48 h later. (E) Intensity quantitation of phospho-p65/RelA from immunoblot experiments. Phosphorylated p65 was normalized internally to total p65, and the unstimulated siCTRL signal was set to 1 for each biological replicate. Data are means ± SEM (n = 3) (one-way ANOVA with a Tukey’s honestly significant difference [HSD] post hoc test), *p < 0.05. (F) RT-PCR and immunoblot for TAK1, the protein product of MAP3K7, after overexpression (OE) of each isoform. (G) ELISA for phosphorylated p65 in keratinocytes after OE of empty vector or MAP3K7 short/long isoforms. Data are means ± SEM (n = 3) (one-way ANOVA with a Tukey’s HSD post hoc test), *p < 0.05.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, In Vitro, In Vivo, Control, Transfection, Western Blot, Quantitation Assay, Over Expression, Enzyme-linked Immunosorbent Assay, Plasmid Preparation

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Blocking Assay, Staining, Lysis, Transfection, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Software, Imaging, Real-time Polymerase Chain Reaction

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Blocking Assay, Staining, Lysis, Transfection, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Software, Imaging, Real-time Polymerase Chain Reaction

    cyclin d1 (e3p5s) xp® rabbit mab  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cyclin d1 (e3p5s) xp® rabbit mab
    Cyclin D1 (E3p5s) Xp® Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1 e3p5s xp rabbit mab cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab cell signaling technology
    Cyclin D1 E3p5s Xp Rabbit Mab Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab

    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion"

    Article Title: Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion

    Journal: iScience

    doi: 10.1016/j.isci.2024.109591


    Figure Legend Snippet:

    Techniques Used: Recombinant, Membrane, BIA-KA, Lactate Assay, Software, Cell Culture, Imaging

    cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab
    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab
    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


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    Antibodies Used for Western Blot Experiments
    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Combination of MDM2 and Targeted Kinase Inhibitors Results in Prolonged Tumor Control in Lung Adenocarcinomas With Oncogenic Tyrosine Kinase Drivers and MDM2 Amplification"

    Article Title: Combination of MDM2 and Targeted Kinase Inhibitors Results in Prolonged Tumor Control in Lung Adenocarcinomas With Oncogenic Tyrosine Kinase Drivers and MDM2 Amplification

    Journal: JCO Precision Oncology

    doi: 10.1200/PO.24.00241

    Antibodies Used for Western Blot Experiments
    Figure Legend Snippet: Antibodies Used for Western Blot Experiments

    Techniques Used: Western Blot

    cyclin d1 e3p5s xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 e3p5s xp rabbit mab
    Cyclin D1 E3p5s Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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