cyclin d1  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

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    rabbit monoclonal anti cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti cyclin d1
    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and <t>cyclin</t> <t>D1,</t> with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Rabbit Monoclonal Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Proteomic insights into the regulatory function of ARID1A in colon cancer cells"

    Article Title: Proteomic insights into the regulatory function of ARID1A in colon cancer cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2024.14525

    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Figure Legend Snippet: Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.

    Techniques Used: Generated, Western Blot, Control, Standard Deviation, Over Expression, Molecular Weight


    Structured Review

    Santa Cruz Biotechnology cyclin d1 cat no sc 8396 rrid ab 627344
    Effects of the combination of CFI-402257 and AICAR on cell cycle related proteins in MDA-MB-231 cells. (A) Western blot analysis of <t>cyclin</t> protein, CDK-related protein and p27. (B) Representative histogram of protein expression level. Data are shown as the mean ± SEM (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. the control group.
    Cyclin D1 Cat No Sc 8396 Rrid Ab 627344, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1 cat no sc 8396 rrid ab 627344/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclin d1 cat no sc 8396 rrid ab 627344 - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Energy‑stress‑mediated activation of AMPK sensitizes MPS1 kinase inhibition in triple‑negative breast cancer"

    Article Title: Energy‑stress‑mediated activation of AMPK sensitizes MPS1 kinase inhibition in triple‑negative breast cancer

    Journal: Oncology Reports

    doi: 10.3892/or.2024.8760

    Effects of the combination of CFI-402257 and AICAR on cell cycle related proteins in MDA-MB-231 cells. (A) Western blot analysis of cyclin protein, CDK-related protein and p27. (B) Representative histogram of protein expression level. Data are shown as the mean ± SEM (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. the control group.
    Figure Legend Snippet: Effects of the combination of CFI-402257 and AICAR on cell cycle related proteins in MDA-MB-231 cells. (A) Western blot analysis of cyclin protein, CDK-related protein and p27. (B) Representative histogram of protein expression level. Data are shown as the mean ± SEM (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. the control group.

    Techniques Used: Western Blot, Expressing, Control


    Structured Review

    Proteintech rabbit anti cyclin d1 antibody
    (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) <t>AAV-GFAP-cyclin</t> <t>D1,</t> and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).
    Rabbit Anti Cyclin D1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cyclin d1 antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cyclin d1 antibody - by Bioz Stars, 2024-07
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    1) Product Images from "Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27 kip1 knockdown promotes retinal regeneration in mice"

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27 kip1 knockdown promotes retinal regeneration in mice

    Journal: bioRxiv

    doi: 10.1101/2024.07.12.603194

    (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) AAV-GFAP-cyclin D1, and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).
    Figure Legend Snippet: (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) AAV-GFAP-cyclin D1, and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).

    Techniques Used: Injection, shRNA, Control, Labeling, Marker, Virus, Infection, Plasmid Preparation


    Structured Review

    ABclonal Biotechnology cyclin d1
    Cyclin D1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/ABclonal Biotechnology
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    cyclin d1 - by Bioz Stars, 2024-07
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    Structured Review

    Santa Cruz Biotechnology cyclin d1
    Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
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    cyclin d1 - by Bioz Stars, 2024-07
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    Structured Review

    Santa Cruz Biotechnology antibody anti cyclin d1
    Antibody Anti Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody anti cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
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    antibody anti cyclin d1 - by Bioz Stars, 2024-07
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    Structured Review

    Santa Cruz Biotechnology cyclin d1
    Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclin d1 - by Bioz Stars, 2024-07
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    Structured Review

    Santa Cruz Biotechnology anti cyclin d1
    ( a–g ) HepG2-shßCat MUT and HepG2-shCtrl cells were treated with doxycycline (DOX) to silence or not mutated ß-catenin. ( a ) Analysis of ß-catenin and CyclinD1 expression by western-blot. Stain-free was used as a loading control. Graphs show the quantification of seven independent experiments. ( b ) Nanoparticle tracking analysis of supernatant. Graphs show the quantification of seven independent experiments. ( c ) Quantification of CD63-pHluorin MVB–PM fusion events visualized by live total internal reflection fluorescence (TIRF) microscopy. Depicted data are representative of three independent experiments, each dot represents one cell. Images represent the cell mask (white) and red dots corresponding to fusion events. Scale bar: 10 µm. ( d ) Extracellular vesicles (EVs) isolation protocol. Created with BioRender.com , and published using a CC BY-NC-ND license with permission ( e ) Analysis of ß-catenin and CD63 expression in HepG2-derived EVs. Stain-free was used as a loading control. Graphs show the quantification of four independent experiments. ( f ) Transmission electron microscopy images of HepG2-derived EVs by close-up. Scale bar: 100 nm. The graph shows the diameter quantification of EVs (n=93). ( g ) Electron microscopy images of HepG2 shCtrl and shßcat MUT cells showing multivesicular bodies (MVBs) (yellow arrowheads). Scale bar: 2 µm (zoom: 500 nm). The graphs show the quantification of the number of multivesicular bodys (MVBs) per cell and the MVB diameter. ( a– g) Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001; ns, non-significant. Figure 2—source data 1. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 2. Original file for the Western blot analysis in <t>(anti-cyclin</t> <t>D1).</t> Figure 2—source data 3. Original file for the Western blot analysis in (stain-free). Figure 2—source data 4. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-cyclin D1, and stain-free) with highlighted bands and sample labels. Figure 2—source data 5. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 6. Original file for the Western blot analysis in (anti-CD63). Figure 2—source data 7. Original file for the Western blot analysis in (stain-free). Figure 2—source data 8. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-CD63, and stain- free) with highlighted bands and sample labels.
    Anti Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cyclin d1 - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma"

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma

    Journal: eLife

    doi: 10.7554/eLife.95191

    ( a–g ) HepG2-shßCat MUT and HepG2-shCtrl cells were treated with doxycycline (DOX) to silence or not mutated ß-catenin. ( a ) Analysis of ß-catenin and CyclinD1 expression by western-blot. Stain-free was used as a loading control. Graphs show the quantification of seven independent experiments. ( b ) Nanoparticle tracking analysis of supernatant. Graphs show the quantification of seven independent experiments. ( c ) Quantification of CD63-pHluorin MVB–PM fusion events visualized by live total internal reflection fluorescence (TIRF) microscopy. Depicted data are representative of three independent experiments, each dot represents one cell. Images represent the cell mask (white) and red dots corresponding to fusion events. Scale bar: 10 µm. ( d ) Extracellular vesicles (EVs) isolation protocol. Created with BioRender.com , and published using a CC BY-NC-ND license with permission ( e ) Analysis of ß-catenin and CD63 expression in HepG2-derived EVs. Stain-free was used as a loading control. Graphs show the quantification of four independent experiments. ( f ) Transmission electron microscopy images of HepG2-derived EVs by close-up. Scale bar: 100 nm. The graph shows the diameter quantification of EVs (n=93). ( g ) Electron microscopy images of HepG2 shCtrl and shßcat MUT cells showing multivesicular bodies (MVBs) (yellow arrowheads). Scale bar: 2 µm (zoom: 500 nm). The graphs show the quantification of the number of multivesicular bodys (MVBs) per cell and the MVB diameter. ( a– g) Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001; ns, non-significant. Figure 2—source data 1. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 2. Original file for the Western blot analysis in (anti-cyclin D1). Figure 2—source data 3. Original file for the Western blot analysis in (stain-free). Figure 2—source data 4. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-cyclin D1, and stain-free) with highlighted bands and sample labels. Figure 2—source data 5. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 6. Original file for the Western blot analysis in (anti-CD63). Figure 2—source data 7. Original file for the Western blot analysis in (stain-free). Figure 2—source data 8. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-CD63, and stain- free) with highlighted bands and sample labels.
    Figure Legend Snippet: ( a–g ) HepG2-shßCat MUT and HepG2-shCtrl cells were treated with doxycycline (DOX) to silence or not mutated ß-catenin. ( a ) Analysis of ß-catenin and CyclinD1 expression by western-blot. Stain-free was used as a loading control. Graphs show the quantification of seven independent experiments. ( b ) Nanoparticle tracking analysis of supernatant. Graphs show the quantification of seven independent experiments. ( c ) Quantification of CD63-pHluorin MVB–PM fusion events visualized by live total internal reflection fluorescence (TIRF) microscopy. Depicted data are representative of three independent experiments, each dot represents one cell. Images represent the cell mask (white) and red dots corresponding to fusion events. Scale bar: 10 µm. ( d ) Extracellular vesicles (EVs) isolation protocol. Created with BioRender.com , and published using a CC BY-NC-ND license with permission ( e ) Analysis of ß-catenin and CD63 expression in HepG2-derived EVs. Stain-free was used as a loading control. Graphs show the quantification of four independent experiments. ( f ) Transmission electron microscopy images of HepG2-derived EVs by close-up. Scale bar: 100 nm. The graph shows the diameter quantification of EVs (n=93). ( g ) Electron microscopy images of HepG2 shCtrl and shßcat MUT cells showing multivesicular bodies (MVBs) (yellow arrowheads). Scale bar: 2 µm (zoom: 500 nm). The graphs show the quantification of the number of multivesicular bodys (MVBs) per cell and the MVB diameter. ( a– g) Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001; ns, non-significant. Figure 2—source data 1. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 2. Original file for the Western blot analysis in (anti-cyclin D1). Figure 2—source data 3. Original file for the Western blot analysis in (stain-free). Figure 2—source data 4. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-cyclin D1, and stain-free) with highlighted bands and sample labels. Figure 2—source data 5. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 6. Original file for the Western blot analysis in (anti-CD63). Figure 2—source data 7. Original file for the Western blot analysis in (stain-free). Figure 2—source data 8. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-CD63, and stain- free) with highlighted bands and sample labels.

    Techniques Used: Expressing, Western Blot, Staining, Control, Fluorescence, Microscopy, Isolation, Derivative Assay, Transmission Assay, Electron Microscopy, Two Tailed Test

    Analysis of CCND1 , AXIN2, and ARG1 mRNA expression by RT-qPCR. HepG2 cells were treated with doxycycline to express either a control shRNA (shCtrl) or a shRNA targeting mutated ß-catenin (shßcat MUT). The graph shows the quantification of six independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. **p<0.01; ***p<0.001.
    Figure Legend Snippet: Analysis of CCND1 , AXIN2, and ARG1 mRNA expression by RT-qPCR. HepG2 cells were treated with doxycycline to express either a control shRNA (shCtrl) or a shRNA targeting mutated ß-catenin (shßcat MUT). The graph shows the quantification of six independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. **p<0.01; ***p<0.001.

    Techniques Used: Expressing, Quantitative RT-PCR, Control, shRNA, Two Tailed Test

    Analysis of CCND1 , AXIN2, and ARG1 mRNA expression in Huh6 and SNU398 shCtrl and shßcat cells treated with doxycycline (DOX). The graph shows the quantification of three independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001.
    Figure Legend Snippet: Analysis of CCND1 , AXIN2, and ARG1 mRNA expression in Huh6 and SNU398 shCtrl and shßcat cells treated with doxycycline (DOX). The graph shows the quantification of three independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001.

    Techniques Used: Expressing, Two Tailed Test

    qPCR primers.
    Figure Legend Snippet: qPCR primers.

    Techniques Used:


    Figure Legend Snippet:

    Techniques Used: Transfection, Construct, Negative Control, Plasmid Preparation, shRNA, Recombinant, Isolation, Sequencing


    Structured Review

    Proteintech cyclin d1
    Cyclin D1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

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    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc rabbit monoclonal anti cyclin d1
    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and <t>cyclin</t> <t>D1,</t> with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.
    Rabbit Monoclonal Anti Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti cyclin d1/product/Cell Signaling Technology Inc
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    Santa Cruz Biotechnology cyclin d1 cat no sc 8396 rrid ab 627344
    Effects of the combination of CFI-402257 and AICAR on cell cycle related proteins in MDA-MB-231 cells. (A) Western blot analysis of <t>cyclin</t> protein, CDK-related protein and p27. (B) Representative histogram of protein expression level. Data are shown as the mean ± SEM (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. the control group.
    Cyclin D1 Cat No Sc 8396 Rrid Ab 627344, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1 cat no sc 8396 rrid ab 627344/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Proteintech rabbit anti cyclin d1 antibody
    (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) <t>AAV-GFAP-cyclin</t> <t>D1,</t> and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).
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    ABclonal Biotechnology cyclin d1
    (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) <t>AAV-GFAP-cyclin</t> <t>D1,</t> and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).
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    Santa Cruz Biotechnology cyclin d1
    (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) <t>AAV-GFAP-cyclin</t> <t>D1,</t> and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).
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    Santa Cruz Biotechnology antibody anti cyclin d1
    (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) <t>AAV-GFAP-cyclin</t> <t>D1,</t> and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).
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    Santa Cruz Biotechnology anti cyclin d1
    ( a–g ) HepG2-shßCat MUT and HepG2-shCtrl cells were treated with doxycycline (DOX) to silence or not mutated ß-catenin. ( a ) Analysis of ß-catenin and CyclinD1 expression by western-blot. Stain-free was used as a loading control. Graphs show the quantification of seven independent experiments. ( b ) Nanoparticle tracking analysis of supernatant. Graphs show the quantification of seven independent experiments. ( c ) Quantification of CD63-pHluorin MVB–PM fusion events visualized by live total internal reflection fluorescence (TIRF) microscopy. Depicted data are representative of three independent experiments, each dot represents one cell. Images represent the cell mask (white) and red dots corresponding to fusion events. Scale bar: 10 µm. ( d ) Extracellular vesicles (EVs) isolation protocol. Created with BioRender.com , and published using a CC BY-NC-ND license with permission ( e ) Analysis of ß-catenin and CD63 expression in HepG2-derived EVs. Stain-free was used as a loading control. Graphs show the quantification of four independent experiments. ( f ) Transmission electron microscopy images of HepG2-derived EVs by close-up. Scale bar: 100 nm. The graph shows the diameter quantification of EVs (n=93). ( g ) Electron microscopy images of HepG2 shCtrl and shßcat MUT cells showing multivesicular bodies (MVBs) (yellow arrowheads). Scale bar: 2 µm (zoom: 500 nm). The graphs show the quantification of the number of multivesicular bodys (MVBs) per cell and the MVB diameter. ( a– g) Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001; ns, non-significant. Figure 2—source data 1. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 2. Original file for the Western blot analysis in <t>(anti-cyclin</t> <t>D1).</t> Figure 2—source data 3. Original file for the Western blot analysis in (stain-free). Figure 2—source data 4. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-cyclin D1, and stain-free) with highlighted bands and sample labels. Figure 2—source data 5. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 6. Original file for the Western blot analysis in (anti-CD63). Figure 2—source data 7. Original file for the Western blot analysis in (stain-free). Figure 2—source data 8. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-CD63, and stain- free) with highlighted bands and sample labels.
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    ( a–g ) HepG2-shßCat MUT and HepG2-shCtrl cells were treated with doxycycline (DOX) to silence or not mutated ß-catenin. ( a ) Analysis of ß-catenin and CyclinD1 expression by western-blot. Stain-free was used as a loading control. Graphs show the quantification of seven independent experiments. ( b ) Nanoparticle tracking analysis of supernatant. Graphs show the quantification of seven independent experiments. ( c ) Quantification of CD63-pHluorin MVB–PM fusion events visualized by live total internal reflection fluorescence (TIRF) microscopy. Depicted data are representative of three independent experiments, each dot represents one cell. Images represent the cell mask (white) and red dots corresponding to fusion events. Scale bar: 10 µm. ( d ) Extracellular vesicles (EVs) isolation protocol. Created with BioRender.com , and published using a CC BY-NC-ND license with permission ( e ) Analysis of ß-catenin and CD63 expression in HepG2-derived EVs. Stain-free was used as a loading control. Graphs show the quantification of four independent experiments. ( f ) Transmission electron microscopy images of HepG2-derived EVs by close-up. Scale bar: 100 nm. The graph shows the diameter quantification of EVs (n=93). ( g ) Electron microscopy images of HepG2 shCtrl and shßcat MUT cells showing multivesicular bodies (MVBs) (yellow arrowheads). Scale bar: 2 µm (zoom: 500 nm). The graphs show the quantification of the number of multivesicular bodys (MVBs) per cell and the MVB diameter. ( a– g) Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001; ns, non-significant. Figure 2—source data 1. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 2. Original file for the Western blot analysis in <t>(anti-cyclin</t> <t>D1).</t> Figure 2—source data 3. Original file for the Western blot analysis in (stain-free). Figure 2—source data 4. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-cyclin D1, and stain-free) with highlighted bands and sample labels. Figure 2—source data 5. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 6. Original file for the Western blot analysis in (anti-CD63). Figure 2—source data 7. Original file for the Western blot analysis in (stain-free). Figure 2—source data 8. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-CD63, and stain- free) with highlighted bands and sample labels.
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    Image Search Results


    Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.

    Journal: Oncology Letters

    Article Title: Proteomic insights into the regulatory function of ARID1A in colon cancer cells

    doi: 10.3892/ol.2024.14525

    Figure Lengend Snippet: Involvement of ARID1A and altered proteins in the Wnt signaling pathway. (A) Wnt signaling pathway information generated using Kyoto Encyclopedia of Genes and Genomes (hsa04310). The pathway is illustrated using data from the KEGG database ( https://www.genome.jp/pathway/hsa04310 ) . Red boxes indicate differentially altered proteins. (B) Western blot images for c-Myc, TCF1/TCF7 and cyclin D1, with GAPDH as the loading control. (C) Band intensity of proteins relative to GAPDH. The bar graph represents the mean ± standard deviation (n=3 per group). *P<0.05. ARID1A, AT-rich interacting domain-containing protein 1A; TCF, T cell factor; O/E, overexpression; MW, molecular weight.

    Article Snippet: The membrane was incubated with 5% skimmed milk in PBS at 25°C for 1 h. Subsequently, it was incubated with rabbit polyclonal anti-ARID1A (1:1,000; cat. no. HPA005456; Sigma-Aldrich; Merck KGaA), rabbit monoclonal anti-c-Myc (1:1,000; cat. no. 5605; Cell Signaling Technology, Inc.), rabbit monoclonal anti-T cell factor (TCF)1/7 (1:1,000; cat. no. 2203; Cell Signaling Technology, Inc.), rabbit monoclonal anti-cyclin D1 (1:1,000; cat. no. 2978; Cell Signaling Technology, Inc.), rabbit polyclonal anti-zinc and ring finger 3 (ZNRF3; 1:1,000; cat. no. DF14289; Affinity Biosciences), or mouse monoclonal anti-GAPDH (1:5,000; cat. no. ab8245; Abcam) antibodies at 4°C overnight.

    Techniques: Generated, Western Blot, Control, Standard Deviation, Over Expression, Molecular Weight

    Effects of the combination of CFI-402257 and AICAR on cell cycle related proteins in MDA-MB-231 cells. (A) Western blot analysis of cyclin protein, CDK-related protein and p27. (B) Representative histogram of protein expression level. Data are shown as the mean ± SEM (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. the control group.

    Journal: Oncology Reports

    Article Title: Energy‑stress‑mediated activation of AMPK sensitizes MPS1 kinase inhibition in triple‑negative breast cancer

    doi: 10.3892/or.2024.8760

    Figure Lengend Snippet: Effects of the combination of CFI-402257 and AICAR on cell cycle related proteins in MDA-MB-231 cells. (A) Western blot analysis of cyclin protein, CDK-related protein and p27. (B) Representative histogram of protein expression level. Data are shown as the mean ± SEM (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. the control group.

    Article Snippet: The primary antibodies for phosphorylated (p-)TTK (cat. no. 44-1325G; RRID: AB_2533594; Thermo Fisher Scientific, Inc.), Cyclin B1 (cat. no. sc-7393; RRID: AB_627336), Cyclin D1 (cat. no. sc-8396; RRID: AB_627344), Cyclin E (cat. no. sc-248; RRID: AB_627362) and p27 (cat. no. sc-1641; RRID: AB_628074; all from Santa Cruz Biotechnology, Inc.) were used.

    Techniques: Western Blot, Expressing, Control

    (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) AAV-GFAP-cyclin D1, and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).

    Journal: bioRxiv

    Article Title: Müller glia cell cycle re-activation by simultaneous cyclin D1 overexpression and p27 kip1 knockdown promotes retinal regeneration in mice

    doi: 10.1101/2024.07.12.603194

    Figure Lengend Snippet: (A) Schematic of AAV vectors used in this study. (B) Experimental design. (C-F) Analysis of EdU incorporation. Uninjured mouse eyes were injected with (C) AAV-GFAP-mCherry-non target (NT) shRNA (control), (D) AAV-GFAP-mCherry-p27 shRNA, (E) AAV-GFAP-cyclin D1, and (F) AAV-GFAP-cyclin D1-p27 shRNA (CCA) at P6 and harvested at P18 after 5-day EdU intraperitoneal injection. Retinal sections were co-labeled for MG marker Sox9. (G) Quantification of EdU+ Sox9+ cells. AAV-GFAP-mCherry-NT shRNA control virus (n=8), AAV-GFAP-mCherry-p27shRNA (n=11), AAV-GFAP-cyclin D1 (n=8), and CCA (n=14). (H) Quantification of the percentages of EdU+GFP+ and EdU-GFP+ cells in the area with efficient virus infection in the Glast-CreERT2; Sun1:GFP mouse retina injected with the control or CCA vector. (I) Quantification of the total GFP+ MG in the CreERT2; Sun1:GFP retina infected by CCA. Retinal area (250 µm in width) with the most efficient virus infection in the middle retina region was selected for quantification. (J) Quantification of EdU+ Sox9+ cells in the young (CCA injection at P6, n=13), adult (CCA injection at P28, n=17), and aged retinas (CCA injection at P255-P347, n=7). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Data are presented as mean ± SEM. * P<0.05; *** P<0.001; ns=not significant by one-way ANOVA analysis with Tukey’s post-hoc test (G, J) and two-tail unpaired student t-test (I).

    Article Snippet: Primary antibodies used in this study included rabbit anti- p27 kip1 (1:200, PA5-16717; Thermofisher); rabbit anti-cyclin D1 antibody (1:300, 26939-1-AP; Proteintech), goat anti-Otx2 antibody (1:200, AF 1979; R&D systems), mouse anti-HuC/D antibody (1:200, A21271; Thermofisher), rabbit anti-GFAP antibody (Z0334, 1:500, DAKO), rabbit anti-Sox2 antibody (1:2000, ab97959; abcam) and rabbit anti-Sox9 antibody (1:1000, AB5535; Millipore).

    Techniques: Injection, shRNA, Control, Labeling, Marker, Virus, Infection, Plasmid Preparation

    ( a–g ) HepG2-shßCat MUT and HepG2-shCtrl cells were treated with doxycycline (DOX) to silence or not mutated ß-catenin. ( a ) Analysis of ß-catenin and CyclinD1 expression by western-blot. Stain-free was used as a loading control. Graphs show the quantification of seven independent experiments. ( b ) Nanoparticle tracking analysis of supernatant. Graphs show the quantification of seven independent experiments. ( c ) Quantification of CD63-pHluorin MVB–PM fusion events visualized by live total internal reflection fluorescence (TIRF) microscopy. Depicted data are representative of three independent experiments, each dot represents one cell. Images represent the cell mask (white) and red dots corresponding to fusion events. Scale bar: 10 µm. ( d ) Extracellular vesicles (EVs) isolation protocol. Created with BioRender.com , and published using a CC BY-NC-ND license with permission ( e ) Analysis of ß-catenin and CD63 expression in HepG2-derived EVs. Stain-free was used as a loading control. Graphs show the quantification of four independent experiments. ( f ) Transmission electron microscopy images of HepG2-derived EVs by close-up. Scale bar: 100 nm. The graph shows the diameter quantification of EVs (n=93). ( g ) Electron microscopy images of HepG2 shCtrl and shßcat MUT cells showing multivesicular bodies (MVBs) (yellow arrowheads). Scale bar: 2 µm (zoom: 500 nm). The graphs show the quantification of the number of multivesicular bodys (MVBs) per cell and the MVB diameter. ( a– g) Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001; ns, non-significant. Figure 2—source data 1. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 2. Original file for the Western blot analysis in (anti-cyclin D1). Figure 2—source data 3. Original file for the Western blot analysis in (stain-free). Figure 2—source data 4. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-cyclin D1, and stain-free) with highlighted bands and sample labels. Figure 2—source data 5. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 6. Original file for the Western blot analysis in (anti-CD63). Figure 2—source data 7. Original file for the Western blot analysis in (stain-free). Figure 2—source data 8. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-CD63, and stain- free) with highlighted bands and sample labels.

    Journal: eLife

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma

    doi: 10.7554/eLife.95191

    Figure Lengend Snippet: ( a–g ) HepG2-shßCat MUT and HepG2-shCtrl cells were treated with doxycycline (DOX) to silence or not mutated ß-catenin. ( a ) Analysis of ß-catenin and CyclinD1 expression by western-blot. Stain-free was used as a loading control. Graphs show the quantification of seven independent experiments. ( b ) Nanoparticle tracking analysis of supernatant. Graphs show the quantification of seven independent experiments. ( c ) Quantification of CD63-pHluorin MVB–PM fusion events visualized by live total internal reflection fluorescence (TIRF) microscopy. Depicted data are representative of three independent experiments, each dot represents one cell. Images represent the cell mask (white) and red dots corresponding to fusion events. Scale bar: 10 µm. ( d ) Extracellular vesicles (EVs) isolation protocol. Created with BioRender.com , and published using a CC BY-NC-ND license with permission ( e ) Analysis of ß-catenin and CD63 expression in HepG2-derived EVs. Stain-free was used as a loading control. Graphs show the quantification of four independent experiments. ( f ) Transmission electron microscopy images of HepG2-derived EVs by close-up. Scale bar: 100 nm. The graph shows the diameter quantification of EVs (n=93). ( g ) Electron microscopy images of HepG2 shCtrl and shßcat MUT cells showing multivesicular bodies (MVBs) (yellow arrowheads). Scale bar: 2 µm (zoom: 500 nm). The graphs show the quantification of the number of multivesicular bodys (MVBs) per cell and the MVB diameter. ( a– g) Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001; ns, non-significant. Figure 2—source data 1. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 2. Original file for the Western blot analysis in (anti-cyclin D1). Figure 2—source data 3. Original file for the Western blot analysis in (stain-free). Figure 2—source data 4. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-cyclin D1, and stain-free) with highlighted bands and sample labels. Figure 2—source data 5. Original file for the Western blot analysis in (anti-ß-catenin). Figure 2—source data 6. Original file for the Western blot analysis in (anti-CD63). Figure 2—source data 7. Original file for the Western blot analysis in (stain-free). Figure 2—source data 8. PDF containing and original scans of the relevant Western blot analysis (anti-ß-catenin, anti-CD63, and stain- free) with highlighted bands and sample labels.

    Article Snippet: Antibody , Anti-Cyclin D1 (mouse monoclonal) , Santa Cruz , sc-20044 , WB: 1/1000.

    Techniques: Expressing, Western Blot, Staining, Control, Fluorescence, Microscopy, Isolation, Derivative Assay, Transmission Assay, Electron Microscopy, Two Tailed Test

    Analysis of CCND1 , AXIN2, and ARG1 mRNA expression by RT-qPCR. HepG2 cells were treated with doxycycline to express either a control shRNA (shCtrl) or a shRNA targeting mutated ß-catenin (shßcat MUT). The graph shows the quantification of six independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. **p<0.01; ***p<0.001.

    Journal: eLife

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma

    doi: 10.7554/eLife.95191

    Figure Lengend Snippet: Analysis of CCND1 , AXIN2, and ARG1 mRNA expression by RT-qPCR. HepG2 cells were treated with doxycycline to express either a control shRNA (shCtrl) or a shRNA targeting mutated ß-catenin (shßcat MUT). The graph shows the quantification of six independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. **p<0.01; ***p<0.001.

    Article Snippet: Antibody , Anti-Cyclin D1 (mouse monoclonal) , Santa Cruz , sc-20044 , WB: 1/1000.

    Techniques: Expressing, Quantitative RT-PCR, Control, shRNA, Two Tailed Test

    Analysis of CCND1 , AXIN2, and ARG1 mRNA expression in Huh6 and SNU398 shCtrl and shßcat cells treated with doxycycline (DOX). The graph shows the quantification of three independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001.

    Journal: eLife

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma

    doi: 10.7554/eLife.95191

    Figure Lengend Snippet: Analysis of CCND1 , AXIN2, and ARG1 mRNA expression in Huh6 and SNU398 shCtrl and shßcat cells treated with doxycycline (DOX). The graph shows the quantification of three independent experiments. Results are expressed as Mean ± SEM, two-tailed Student’s t-test analysis. *p<0.05; **p<0.01; ***p<0.001.

    Article Snippet: Antibody , Anti-Cyclin D1 (mouse monoclonal) , Santa Cruz , sc-20044 , WB: 1/1000.

    Techniques: Expressing, Two Tailed Test

    qPCR primers.

    Journal: eLife

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma

    doi: 10.7554/eLife.95191

    Figure Lengend Snippet: qPCR primers.

    Article Snippet: Antibody , Anti-Cyclin D1 (mouse monoclonal) , Santa Cruz , sc-20044 , WB: 1/1000.

    Techniques:

    Journal: eLife

    Article Title: Emerging role of oncogenic ß-catenin in exosome biogenesis as a driver of immune escape in hepatocellular carcinoma

    doi: 10.7554/eLife.95191

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-Cyclin D1 (mouse monoclonal) , Santa Cruz , sc-20044 , WB: 1/1000.

    Techniques: Transfection, Construct, Negative Control, Plasmid Preparation, shRNA, Recombinant, Isolation, Sequencing