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Bio-Rad cycler
Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Polymerase Chain Reaction:

Article Title: Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells
Article Snippet: Each tube was treated with control vehicle (DMSO) or 1 μM methotrexate, respectively, and incubated in 37°C, 5% CO2 incubator for 30 min. .. Cell suspensions were distributed into PCR tubes (25 μl/tube, 8 tubes) and heated for 3 min using a gradient PCR condition (38, 39.7, 42.6, 47.3, 52.8, 57.3, 60.5, 62.5°C; T100 Thermal Cycler, Bio-rad). ..

Article Title: Breast Tumor Cell Invasion and Pro-Invasive Activity of Cancer-Associated Fibroblasts Co-Targeted by Novel Urokinase-Derived Decapeptides
Article Snippet: .. 50 ng of DNA were used as template for Semi-Quantitative PCR reactions (T100 Thermal Cycler, Bio-Rad Laboratories, Milan, Italy). .. The DNA was incubated in a total reaction volume of 20 μL, containing 1× Reaction Buffer, Taq DNA polymerase (Cat #EME010001, EuroClone, Milan, Italy), 200 μM dNTP mixture and 5 mM MgCl2 .

Article Title: Droplet digital PCR is an accurate method to assess methylation status on FFPE samples
Article Snippet: .. After PCR amplification using the T100™ Thermal Cycler Bio-Rad, the positive droplets (HEX/FAM) were counted with QX200 droplet reader (Bio-Rad) and data was analyzed using the QuantaSoft 1.7.4 software (Bio-Rad). .. The total number of copies/reaction was calculated and results were reported as the percentage methylation, i.e., the fractional abundance of methylated DNA alleles.

Article Title: Development of loop-mediated isothermal amplification with Plasmodium falciparum unique genes for molecular diagnosis of human malaria
Article Snippet: The reaction mixture in a 0.2-ml PCR tube was set up by adding 12.5 μl 2× reaction buffer, 1.0 μl Bst DNA polymerase, 1.0 μl genomic DNA, 1.0 μl working LAMP primer mixture, and 9.5 μl sterile ultrapure water. .. Finally, isometric mineral oil (20 μl) was added into the PCR tube, which was then centrifuged for 30 s. The LAMP reaction tubes were incubated at 63 °C for 60 min in a T100™ Thermal Cycler (BIO-RAD, Singapore). ..

Isolation:

Article Title: The Anti-Inflammatory Compound Curcumin Enhances Locomotor and Sensory Recovery after Spinal Cord Injury in Rats by Immunomodulation
Article Snippet: A spectrophotometer (NanoPhotometerTM P-Class, Munchen, Germany) was used to quantify RNA amounts. .. The isolated RNA was reverse transcribed into cDNA using Transcriptor Universal cDNA Master (Roche, Penzberg, Germany) and a thermal cycler (T100™ Thermal Cycler, Bio-Rad, Hercules, CA, USA). .. The qPCR reactions were performed using a cDNA solution, FastStart Universal Probe Master (Roche, Penzberg, Germany) and TaqMan® Gene Expression Assays (Life Technologies, Carlsbad, CA, USA) ( ).

Synthesized:

Article Title: Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion – A multicomponent metabolomic analysis
Article Snippet: Total RNA was quantified using a nanophotometer readings at 260 and 280 nm (NanoPhotometerTM Pearl, Implen GmbH). .. Once RNA concentrations were tuned between samples, cDNA was synthesized from the purified RNA using the iScript Reverse Transcription kit (170–8891, Bio Rad) and T100 Terma Cyclar Thermocycler (Bio Rad), as per manufacturer’s instructions. .. Quantitative PCR (qPCR) was performed in a CFX96 TouchTM (BioRad) apparatus using the iTAQTM SYBR® Green Supermix (172–5120, BioRad) and custom PCR plates encompassing duplicates of targeted human genes and negative control ( ).

Purification:

Article Title: Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion – A multicomponent metabolomic analysis
Article Snippet: Total RNA was quantified using a nanophotometer readings at 260 and 280 nm (NanoPhotometerTM Pearl, Implen GmbH). .. Once RNA concentrations were tuned between samples, cDNA was synthesized from the purified RNA using the iScript Reverse Transcription kit (170–8891, Bio Rad) and T100 Terma Cyclar Thermocycler (Bio Rad), as per manufacturer’s instructions. .. Quantitative PCR (qPCR) was performed in a CFX96 TouchTM (BioRad) apparatus using the iTAQTM SYBR® Green Supermix (172–5120, BioRad) and custom PCR plates encompassing duplicates of targeted human genes and negative control ( ).

Article Title: The effects of macrophages on cardiomyocyte calcium‐handling function using in vitro culture models. The effects of macrophages on cardiomyocyte calcium‐handling function using in vitro culture models
Article Snippet: Culture medium of macrophage and cardiomyocyte was then mixed in a 1:1 ratio to initiate the co‐culture. .. RT‐PCR Total RNA was extracted and purified from mES‐CM and macrophages using the GenElute Mammalian Total RNA Miniprep Kit following the manufacturer's instruction (Fisher Scientific). cDNA was created with 500 ng of RNA and the High‐Capacity cDNA Reverse Transcription Kit (Applied Biosystems) in the T100 Thermal Cycler (Bio‐rad). .. RT‐PCR reactions were then prepared with the SSo Advanced SYBRgreen Mastermix (Bio‐rad).

Ligation:

Article Title: Ecological influence of sediment bypass tunnels on macroinvertebrates in dam-fragmented rivers by DNA metabarcoding
Article Snippet: The adapters were ligated to the adenilated products in a 50 µl ligation mixture consisting of 5 µl ligation buffer (TaKaRa), 1 µl T4 DNA ligase (TaKaRa), 40 µl of adenylated product, annealed adapter (100× of mol amount of the adenylated product) and PCR-grade water. .. Ligation was performed for 19 hours at 16 °C and 65 °C for 2 min in a T100TM Thermal Cycler (BioRad). .. Ligated products were purified using Agencourt AmpureXP (Beckman Coulter).

Reverse Transcription Polymerase Chain Reaction:

Article Title: The effects of macrophages on cardiomyocyte calcium‐handling function using in vitro culture models. The effects of macrophages on cardiomyocyte calcium‐handling function using in vitro culture models
Article Snippet: Culture medium of macrophage and cardiomyocyte was then mixed in a 1:1 ratio to initiate the co‐culture. .. RT‐PCR Total RNA was extracted and purified from mES‐CM and macrophages using the GenElute Mammalian Total RNA Miniprep Kit following the manufacturer's instruction (Fisher Scientific). cDNA was created with 500 ng of RNA and the High‐Capacity cDNA Reverse Transcription Kit (Applied Biosystems) in the T100 Thermal Cycler (Bio‐rad). .. RT‐PCR reactions were then prepared with the SSo Advanced SYBRgreen Mastermix (Bio‐rad).

Amplification:

Article Title: Droplet digital PCR is an accurate method to assess methylation status on FFPE samples
Article Snippet: .. After PCR amplification using the T100™ Thermal Cycler Bio-Rad, the positive droplets (HEX/FAM) were counted with QX200 droplet reader (Bio-Rad) and data was analyzed using the QuantaSoft 1.7.4 software (Bio-Rad). .. The total number of copies/reaction was calculated and results were reported as the percentage methylation, i.e., the fractional abundance of methylated DNA alleles.

Software:

Article Title: Droplet digital PCR is an accurate method to assess methylation status on FFPE samples
Article Snippet: .. After PCR amplification using the T100™ Thermal Cycler Bio-Rad, the positive droplets (HEX/FAM) were counted with QX200 droplet reader (Bio-Rad) and data was analyzed using the QuantaSoft 1.7.4 software (Bio-Rad). .. The total number of copies/reaction was calculated and results were reported as the percentage methylation, i.e., the fractional abundance of methylated DNA alleles.

Incubation:

Article Title: Development of loop-mediated isothermal amplification with Plasmodium falciparum unique genes for molecular diagnosis of human malaria
Article Snippet: The reaction mixture in a 0.2-ml PCR tube was set up by adding 12.5 μl 2× reaction buffer, 1.0 μl Bst DNA polymerase, 1.0 μl genomic DNA, 1.0 μl working LAMP primer mixture, and 9.5 μl sterile ultrapure water. .. Finally, isometric mineral oil (20 μl) was added into the PCR tube, which was then centrifuged for 30 s. The LAMP reaction tubes were incubated at 63 °C for 60 min in a T100™ Thermal Cycler (BIO-RAD, Singapore). ..

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  • 98
    Bio-Rad dna thermal cycler
    <t>DNA</t> manipulation and <t>PCR</t> amplification.
    Dna Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna thermal cycler/product/Bio-Rad
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    99
    Bio-Rad qpcr cycler
    Altered T cell immunity after exposure to MNV4 in vitro . Fecal pellets from MNV4-infected 12-week old GF NOD were homogenized and filtrate was cultured with splenocytes from MNV4- or MNV4-colonized GF NOD mice. Cells were stimulated for 12 h prior to the addition of PMA, Ionomycin and GolgiPlug for a further 4 h preceding the surface and intracellular staining. As a control, splenocytes were not stimulated with MNV4-containing fecal filtrate but were stimulated with PMA, Ionomycin and GolgiPlug. (A,B) The proportion of CD69+ CD4 T cells (A) and CD8 T cells (B) were gated from live, single TCRbeta+CD19- cells prior to gating on CD4 or CD8 respectively and then CD69. (C,D) CD4+FoxP3+ Tregs were investigated for CD69+ (C) and CTLA4+ (D) cells. Tregs were gated as in B, prior to FoxP3 gating and then CD69 or CTLA4 gating. (E–G) The proportion of IFNγ-secreting CD8 T cells (E) and TNFα-secreting CD4 T cells (F) and CD8 T cells (G) were gated as in A,B prior to subsequent gating on IFNγ or TNFα. RNA from cultured T cells or APCs was isolated following exposure to UV-treated or UV-untreated (MNV4-containing) fecal material. Equal concentrations of <t>cDNA</t> were synthesized and then subjected to <t>qPCR</t> for MNV4 (H,I) . The relative expressions of these genes were determined using the 2 −ΔΔCT method by normalization with GAPDH. MNV4-exposed or MNV4-naive splenocytes were adoptively transferred into Rag-deficient NOD mice (4 × 10 6 /donor). Seven-days later mice were sacrificed and Tregs were investigated by flow cytometric analysis. Tregs were gated from live, single cell, CD4+TCRbeta+Foxp3+ T cells. Treg number (J) and the proportion of CD39- (K) , CCR6- (L) , CCR7- (M) and CCR9-expressing (N) Tregs are shown. All data were analyzed for significance using a Student's T -test. Data shown are representative of one of two experiments, averaged from experimental duplicates with n = 4 per group/experiment. * P
    Qpcr Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qpcr cycler/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
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    97
    Bio-Rad ptc 200 peltier thermal cycler
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Ptc 200 Peltier Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DNA manipulation and PCR amplification.

    Journal: Applied and Environmental Microbiology

    Article Title: Sequencing and Transcriptional Analysis of the Biosynthesis Gene Cluster of Putrescine-Producing Lactococcus lactis ▿ ▿ †

    doi: 10.1128/AEM.05507-11

    Figure Lengend Snippet: DNA manipulation and PCR amplification.

    Article Snippet: PCR amplification was performed using a DNA thermal cycler (iCycler; Bio-Rad, Hercules, CA) for 35 cycles with the general program (94°C for 30 s, 25 s at the suitable annealing temperature of the primers used, and 72°C for 1 min per kb of DNA to be amplified).

    Techniques: Polymerase Chain Reaction, Amplification

    Altered T cell immunity after exposure to MNV4 in vitro . Fecal pellets from MNV4-infected 12-week old GF NOD were homogenized and filtrate was cultured with splenocytes from MNV4- or MNV4-colonized GF NOD mice. Cells were stimulated for 12 h prior to the addition of PMA, Ionomycin and GolgiPlug for a further 4 h preceding the surface and intracellular staining. As a control, splenocytes were not stimulated with MNV4-containing fecal filtrate but were stimulated with PMA, Ionomycin and GolgiPlug. (A,B) The proportion of CD69+ CD4 T cells (A) and CD8 T cells (B) were gated from live, single TCRbeta+CD19- cells prior to gating on CD4 or CD8 respectively and then CD69. (C,D) CD4+FoxP3+ Tregs were investigated for CD69+ (C) and CTLA4+ (D) cells. Tregs were gated as in B, prior to FoxP3 gating and then CD69 or CTLA4 gating. (E–G) The proportion of IFNγ-secreting CD8 T cells (E) and TNFα-secreting CD4 T cells (F) and CD8 T cells (G) were gated as in A,B prior to subsequent gating on IFNγ or TNFα. RNA from cultured T cells or APCs was isolated following exposure to UV-treated or UV-untreated (MNV4-containing) fecal material. Equal concentrations of cDNA were synthesized and then subjected to qPCR for MNV4 (H,I) . The relative expressions of these genes were determined using the 2 −ΔΔCT method by normalization with GAPDH. MNV4-exposed or MNV4-naive splenocytes were adoptively transferred into Rag-deficient NOD mice (4 × 10 6 /donor). Seven-days later mice were sacrificed and Tregs were investigated by flow cytometric analysis. Tregs were gated from live, single cell, CD4+TCRbeta+Foxp3+ T cells. Treg number (J) and the proportion of CD39- (K) , CCR6- (L) , CCR7- (M) and CCR9-expressing (N) Tregs are shown. All data were analyzed for significance using a Student's T -test. Data shown are representative of one of two experiments, averaged from experimental duplicates with n = 4 per group/experiment. * P

    Journal: Frontiers in Immunology

    Article Title: Norovirus Changes Susceptibility to Type 1 Diabetes by Altering Intestinal Microbiota and Immune Cell Functions

    doi: 10.3389/fimmu.2019.02654

    Figure Lengend Snippet: Altered T cell immunity after exposure to MNV4 in vitro . Fecal pellets from MNV4-infected 12-week old GF NOD were homogenized and filtrate was cultured with splenocytes from MNV4- or MNV4-colonized GF NOD mice. Cells were stimulated for 12 h prior to the addition of PMA, Ionomycin and GolgiPlug for a further 4 h preceding the surface and intracellular staining. As a control, splenocytes were not stimulated with MNV4-containing fecal filtrate but were stimulated with PMA, Ionomycin and GolgiPlug. (A,B) The proportion of CD69+ CD4 T cells (A) and CD8 T cells (B) were gated from live, single TCRbeta+CD19- cells prior to gating on CD4 or CD8 respectively and then CD69. (C,D) CD4+FoxP3+ Tregs were investigated for CD69+ (C) and CTLA4+ (D) cells. Tregs were gated as in B, prior to FoxP3 gating and then CD69 or CTLA4 gating. (E–G) The proportion of IFNγ-secreting CD8 T cells (E) and TNFα-secreting CD4 T cells (F) and CD8 T cells (G) were gated as in A,B prior to subsequent gating on IFNγ or TNFα. RNA from cultured T cells or APCs was isolated following exposure to UV-treated or UV-untreated (MNV4-containing) fecal material. Equal concentrations of cDNA were synthesized and then subjected to qPCR for MNV4 (H,I) . The relative expressions of these genes were determined using the 2 −ΔΔCT method by normalization with GAPDH. MNV4-exposed or MNV4-naive splenocytes were adoptively transferred into Rag-deficient NOD mice (4 × 10 6 /donor). Seven-days later mice were sacrificed and Tregs were investigated by flow cytometric analysis. Tregs were gated from live, single cell, CD4+TCRbeta+Foxp3+ T cells. Treg number (J) and the proportion of CD39- (K) , CCR6- (L) , CCR7- (M) and CCR9-expressing (N) Tregs are shown. All data were analyzed for significance using a Student's T -test. Data shown are representative of one of two experiments, averaged from experimental duplicates with n = 4 per group/experiment. * P

    Article Snippet: qPCR RNA was extracted from the distal small intestine of colonized GF NOD mice, using a Qiagen RNAeasy kit, prior to cDNA synthesis following the manufacturer's instructions (Bio-Rad). qPCR was performed using a qPCR cycler (iQ5; Bio-Rad Laboratories), according to the manufacturer's instructions with the specific primers listed in .

    Techniques: In Vitro, Infection, Cell Culture, Mouse Assay, Staining, Isolation, Synthesized, Real-time Polymerase Chain Reaction, Flow Cytometry, Expressing

    Altered intestinal immunity in norovirus infected NOD mice. GF NOD mice were orally gavaged at ~4 weeks of age with pooled and filtered norovirus-enriched fecal filtrate from norovirus-infected NOD mice at 12-weeks of age that was UV-treated (virus–) or non-UV-treated (virus+). Only mice gavaged with the non-UV-treated filtrate were infected with norovirus and developed anti-MNV antibodies. (A) Cytokines in cecal flushes from infected/non-infected mice were measured by ELISA. (B–N) RNA from the distal small intestine immediately adjacent to the cecum was extracted and equal concentrations of cDNA synthesized. cDNA was then subject to qPCR for MNV4, genes associated with Tuft cells [doublecortin-like kinase 1 (dclk1), IL25, succinate receptor 1; C–E respectively], with Toll-like receptor (TLR) genes (tlr7 and tlr8; F,G respectively) and antiviral immune signaling genes [Interferon α receptor 1 (ifnar1), interferon lamda (ifnλ), retinoic inducible gene I (rigI), signal transducer and activator of transcription 1 (stat1), nuclear factor kappa-light-chain-enhancer of activated B cells (nfκb), interferon regulatory factor (IRF) 1 and 3; H–M respectively]. The relative expressions of these genes were determined using the 2 −ΔΔCT method by normalization with GAPDH. Data were assessed for significance using a Student's T -test (A–N) . Data shown are representative of one of two experiments with n = 4 per group/experiment. * P

    Journal: Frontiers in Immunology

    Article Title: Norovirus Changes Susceptibility to Type 1 Diabetes by Altering Intestinal Microbiota and Immune Cell Functions

    doi: 10.3389/fimmu.2019.02654

    Figure Lengend Snippet: Altered intestinal immunity in norovirus infected NOD mice. GF NOD mice were orally gavaged at ~4 weeks of age with pooled and filtered norovirus-enriched fecal filtrate from norovirus-infected NOD mice at 12-weeks of age that was UV-treated (virus–) or non-UV-treated (virus+). Only mice gavaged with the non-UV-treated filtrate were infected with norovirus and developed anti-MNV antibodies. (A) Cytokines in cecal flushes from infected/non-infected mice were measured by ELISA. (B–N) RNA from the distal small intestine immediately adjacent to the cecum was extracted and equal concentrations of cDNA synthesized. cDNA was then subject to qPCR for MNV4, genes associated with Tuft cells [doublecortin-like kinase 1 (dclk1), IL25, succinate receptor 1; C–E respectively], with Toll-like receptor (TLR) genes (tlr7 and tlr8; F,G respectively) and antiviral immune signaling genes [Interferon α receptor 1 (ifnar1), interferon lamda (ifnλ), retinoic inducible gene I (rigI), signal transducer and activator of transcription 1 (stat1), nuclear factor kappa-light-chain-enhancer of activated B cells (nfκb), interferon regulatory factor (IRF) 1 and 3; H–M respectively]. The relative expressions of these genes were determined using the 2 −ΔΔCT method by normalization with GAPDH. Data were assessed for significance using a Student's T -test (A–N) . Data shown are representative of one of two experiments with n = 4 per group/experiment. * P

    Article Snippet: qPCR RNA was extracted from the distal small intestine of colonized GF NOD mice, using a Qiagen RNAeasy kit, prior to cDNA synthesis following the manufacturer's instructions (Bio-Rad). qPCR was performed using a qPCR cycler (iQ5; Bio-Rad Laboratories), according to the manufacturer's instructions with the specific primers listed in .

    Techniques: Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay, Synthesized, Real-time Polymerase Chain Reaction

    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum

    Journal: Pharmacognosy Magazine

    Article Title: Specific PCR Identification between Peucedanum praeruptorum and Angelica decursiva and Identification between Them and Adulterant Using DNA Barcode

    doi: 10.4103/0973-1296.197658

    Figure Lengend Snippet: Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum

    Article Snippet: Instruments and reagents PCR instrument: MJ Research PTC-200 Peltier Thermal Cycler (Bio-Rad company), Tprofessional standard Thermocycler (Biometra company) and Light Cycler® 96 System (Roche company); Mikro 120 type microcentrifuge (Hettich company); BG-Power 600k electrophoresis apparatus (Beijing Baijing Biotechnology Co., Ltd.); automatic gel imaging analyzer (Beijing Baijing biotechnology limited company); UV-2102 PCS type ultraviolet visible spectrophotometer [Unique (Shanghai) Instrument Co. Ltd.]; WH-3 type vortex oscillator (Shanghai Huxi analytical instrument Factory Co. Ltd.); SYQ-DSX-280B type portable stainless steel autoclave (Shanghai Shenan medical instrument factory).

    Techniques: Polymerase Chain Reaction, Marker, Amplification, Negative Control

    Optimization of different influence factors in specific PCR of P. praeruptorum . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using P. praeruptorum identification primer pairs QH-CP19s\QH-CP19a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B . Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of P. praeruptorum with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates P. praeruptorum , and 2 indicates A. decursiva . F. PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates P. praeruptorum , and 2 indicates A. decursiva .

    Journal: Pharmacognosy Magazine

    Article Title: Specific PCR Identification between Peucedanum praeruptorum and Angelica decursiva and Identification between Them and Adulterant Using DNA Barcode

    doi: 10.4103/0973-1296.197658

    Figure Lengend Snippet: Optimization of different influence factors in specific PCR of P. praeruptorum . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using P. praeruptorum identification primer pairs QH-CP19s\QH-CP19a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B . Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of P. praeruptorum with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates P. praeruptorum , and 2 indicates A. decursiva . F. PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates P. praeruptorum , and 2 indicates A. decursiva .

    Article Snippet: Instruments and reagents PCR instrument: MJ Research PTC-200 Peltier Thermal Cycler (Bio-Rad company), Tprofessional standard Thermocycler (Biometra company) and Light Cycler® 96 System (Roche company); Mikro 120 type microcentrifuge (Hettich company); BG-Power 600k electrophoresis apparatus (Beijing Baijing Biotechnology Co., Ltd.); automatic gel imaging analyzer (Beijing Baijing biotechnology limited company); UV-2102 PCS type ultraviolet visible spectrophotometer [Unique (Shanghai) Instrument Co. Ltd.]; WH-3 type vortex oscillator (Shanghai Huxi analytical instrument Factory Co. Ltd.); SYQ-DSX-280B type portable stainless steel autoclave (Shanghai Shenan medical instrument factory).

    Techniques: Polymerase Chain Reaction, Marker, Amplification, Negative Control