cy3 goat anti rabbit ab  (Jackson Immuno)

 
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    Name:
    Cy 3 AffiniPure F ab ₂ Fragment Goat Anti Rabbit IgG Fc fragment specific
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    111-166-046
    Price:
    229.0
    Category:
    F ab ₂ Fragment Affinity Purified Antibodies
    Conjugate:
    Cyanine Cy 3
    Size:
    1 0 mg
    Format:
    F(ab')₂ Fragment
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Jackson Immuno cy3 goat anti rabbit ab
    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat <t>Cy3</t> <t>anti-rabbit</t> Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/cy3 goat anti rabbit ab/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 goat anti rabbit ab - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Regulated Targeting of BAX to Mitochondria "

    Article Title: Regulated Targeting of BAX to Mitochondria

    Journal: The Journal of Cell Biology

    doi:

    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).
    Figure Legend Snippet: Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).

    Techniques Used: Confocal Microscopy, Labeling, Fluorescence, Staining

    Related Articles

    other:

    Article Title: Ionotropic Receptor-dependent cool cells control the transition of temperature preference in Drosophila larvae
    Article Snippet: The following antibodies were used: guinea pig anti-IR21a [ ] (1:100), guinea pig anti-IR93a [ ] (1:100), rabbit anti-IR25a [ ] (1:100), chicken anti-GFP (1:500; Abcam), goat anti-guinea pig Cy3 (1:100; Jackson ImmunoResearch), goat anti-rabbit Cy3 (1:100; Jackson ImmunoResearch), goat anti-rabbit FITC (1:100; Jackson ImmunoResearch), goat anti-chicken FITC (1:500; Invitrogene).

    Article Title: Actin bundles play a different role in shaping scales compared to bristles in the mosquito Aedes aegypti
    Article Snippet: Goat anti-mouse Cy2 and Cy3 and goat anti-rabbit Cy3 (Jackson ImmunoResearch) secondary antibodies were used at a dilution of 1:100.

    Article Title: Oocyte Aging is Controlled by Mitogen Activated Protein Kinase Signaling
    Article Snippet: The primary antibodies used were as follow: rabbit α-LAB-1 (1:200, ( )), rabbit α-RAD-51 (1:10,000, SDIX), mouse α-MAPK-YT (1:500, M8159; Sigma), rabbit α-SYP-2 (1:200, a kind gift from S. Smolikove), rabbit α-pH3 (D5692, 1:1000; Sigma), and guinea pig α-HTP-3 (1:200, ( )).

    Article Title: Systematic functional analysis of rab GTPases reveals limits of neuronal robustness to environmental challenges in flies
    Article Snippet: Secondary antibodies used were Donkey anti-mouse DyLight 405, Goat anti-mouse Alexa 488, Goat anti-guinea pig Alexa 488, Goat anti-rat Alexa 488, Goat anti-rabbit Cy3, Goat anti-rabbit Alexa 647, Goat anti-rat Alexa 647, Goat anti-mouse Cy5, Goat anti-rat Cy5 (1:500; Jackson ImmunoResearch Laboratories), Goat anti-guinea pig HRP-linked (1:5000, Jackson ImmunoResearch Laboratories), Goat anti-guinea pig Cy5 (1:500, Abcam), Phalloidin-iFluor 405 (1:250; Abcam) and Toto-3 (1:1000; Thermo Fisher Scientific).

    Article Title: Oocyte Aging is Controlled by Mitogen Activated Protein Kinase Signaling
    Article Snippet: The secondary antibodies used were Cy2-donkey anti-rabbit, Cy3-donkey anti-guinea pig, Cy3-goat anti-rabbit, Cy3-goat anti-mouse (all used at 1:500 dilution; Jackson ImmunoResearch Laboratories).

    Article Title: Exposure to bisphenol A differentially impacts neurodevelopment and behavior in Drosophila melanogaster from distinct genetic backgrounds
    Article Snippet: Antibodies for neuroblast (NB) and intermediate neural progenitor (INP) detection were used at the following dilutions in 0.3% PBST (PBS + 10% Triton-X 100): Rabbit anti-phospho-Histone H3 (pH3; Cell Signaling) at 1:500, rat anti-Deadpan (Abcam) at 1:75, goat anti-rabbit Cy3 (Jackson Immunoresearch) at 1:300, and goat anti-rat Alexa 488 (Jackson ImmunoResearch) at 1:250.

    Double Staining:

    Article Title: Calcium mediated functional interplay between myocardial cells upon laser-induced single-cell injury: an in vitro study of cardiac cell death signaling mechanisms
    Article Snippet: .. For double staining of sarcomeres and connexin43, Alexa 488 goat anti-mouse (A11001, Invitrogen, Carlsbad, CA) and Cy3-conjugated goat anti-rabbit (111-166-046, Jackson Immunoresearch) secondary antibodies were used. ..

    Labeling:

    Article Title: Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds
    Article Snippet: .. Following antibodies and affinity probes were used for immunocytochemical labeling: rabbit anti–NMHC-IIA (BioLegend), goat anti-rabbit Cy3 (Jackson ImmunoResearch), phalloidin–Alexa Fluor 488 (Thermo Fisher Scientific), and 4′,6-diamidino-2-phenylindole (DAPI) (Carl Roth). ..

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  • 94
    Jackson Immuno cy3 goat anti rabbit ab
    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat <t>Cy3</t> <t>anti-rabbit</t> Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).
    Cy3 Goat Anti Rabbit Ab, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 goat anti rabbit ab/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 goat anti rabbit ab - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    88
    Jackson Immuno anti cy3 goat anti rabbit secondary antibody
    Cx50 mutant subunits differ in their ability to localize to the plasma membrane. Transiently transfected HeLa cells expressing WT Cx50 ( A ), V44E ( B ), D47N ( C ), and V79L ( D ) proteins were immunostained and examined by fluorescence microscopy. Merged images of <t>Cy3</t> (red) staining of connexins and DAPI staining of cell nuclei (blue) were taken at ×40 magnification. D47N failed to correctly localize to cell membrane appositions, whereas V44E and V79L formed gap junction plaques at areas of cell-cell contact similar to WT Cx50.
    Anti Cy3 Goat Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cy3 goat anti rabbit secondary antibody/product/Jackson Immuno
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cy3 goat anti rabbit secondary antibody - by Bioz Stars, 2021-09
    88/100 stars
      Buy from Supplier

    93
    Jackson Immuno goat anti rabbit cy3 secondary antibody
    Lhx6-EGFP is reliably expressed in fast-spiking perisomatic-targeting PV + cells. A , Confocal image stack from a horizontal section (50 μm) through the DG of a juvenile Lhx6-EGFP mouse. Note the high density of Lhx6-EGFP-positive somata at the gcl–hilus border (arrows). Inset, Confocal image demonstrating colocalization of EGFP and Lhx6 detected with a primary antibody and visualized using a secondary antibody conjugated to <t>Cy3.</t> Scale bar, 20 μm. B , Left, A single Lhx6-EGFP-positive neuron was filled with biocytin during whole-cell recording and visualized subsequently. The dense axonal arborizations in the gcl (arrows) identify this neuron as a perisomatic-targeting cell. The selected somatic area (red box) is shown on the right at higher magnification. Inset, Perisomatic-targeting cells had a typical fast-spiking non-adapting discharge pattern (600 pA, 1 s; V hold −70 mV). Right, Confocal image stack of the same cell stained against parvalbumin, a characteristic marker for perisomatic-targeting cells. Scale bar, 10 μm. ml, Molecular layer.
    Goat Anti Rabbit Cy3 Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit cy3 secondary antibody/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit cy3 secondary antibody - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).

    Journal: The Journal of Cell Biology

    Article Title: Regulated Targeting of BAX to Mitochondria

    doi:

    Figure Lengend Snippet: Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).

    Article Snippet: Anti-mBAX (P-19) Ab ( Santa Cruz Biotechnology , Santa Cruz, CA) and Cy3 goat anti–rabbit Ab (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to detect BAX immunoreactivity.

    Techniques: Confocal Microscopy, Labeling, Fluorescence, Staining

    Wnt-1, β-catenin and E-cadherin proteins localize predominantly in the hepatocytes of an adult rat liver. (A) Western blot demonstrates wnt-1 in hepatocytes of a resting adult liver. (B) β-Catenin is seen in hepatocytes and Kupffer cells. (C) Immunofluorescence staining reveals localizing of β-catenin ( green ) on the membranes and cytoplasm (immediately next to the membrane) in the rat liver uniformly. Some hepatocytes show nuclear localization ( arrowheads ). Inset reveals nuclear localization as depicted by the yellow color by overlay ( arrowhead ) of Cy3 ( red ) for β-catenin and Sytox Green for the nuclei. Bar = 10 μm. (D) E-cadherin ( red ) localizes to the membrane ( arrowheads ) and the cytoplasm (immediate proximity to the membrane) of the hepatocytes in a normal rat liver. Bar = 10 μm.

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Changes in Wnt/?-Catenin Pathway During Regulated Growth in Rat Liver Regeneration

    doi: 10.1053/jhep.2001.23786

    Figure Lengend Snippet: Wnt-1, β-catenin and E-cadherin proteins localize predominantly in the hepatocytes of an adult rat liver. (A) Western blot demonstrates wnt-1 in hepatocytes of a resting adult liver. (B) β-Catenin is seen in hepatocytes and Kupffer cells. (C) Immunofluorescence staining reveals localizing of β-catenin ( green ) on the membranes and cytoplasm (immediately next to the membrane) in the rat liver uniformly. Some hepatocytes show nuclear localization ( arrowheads ). Inset reveals nuclear localization as depicted by the yellow color by overlay ( arrowhead ) of Cy3 ( red ) for β-catenin and Sytox Green for the nuclei. Bar = 10 μm. (D) E-cadherin ( red ) localizes to the membrane ( arrowheads ) and the cytoplasm (immediate proximity to the membrane) of the hepatocytes in a normal rat liver. Bar = 10 μm.

    Article Snippet: Secondary antibodies used for this study were goat anti-rabbit Cy3, goat anti-mouse Cy3 (Jackson ImmunoResearch Laboratories, West Grove, PA), or Alexa 488 (Molecular Probes, Eugene, OR) at a 1:3,000 (for Cy3) or 1:500 (for Alexa 488) dilution.

    Techniques: Western Blot, Immunofluorescence, Staining

    Cx50 mutant subunits differ in their ability to localize to the plasma membrane. Transiently transfected HeLa cells expressing WT Cx50 ( A ), V44E ( B ), D47N ( C ), and V79L ( D ) proteins were immunostained and examined by fluorescence microscopy. Merged images of Cy3 (red) staining of connexins and DAPI staining of cell nuclei (blue) were taken at ×40 magnification. D47N failed to correctly localize to cell membrane appositions, whereas V44E and V79L formed gap junction plaques at areas of cell-cell contact similar to WT Cx50.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Functional effects of Cx50 mutations associated with congenital cataracts

    doi: 10.1152/ajpcell.00098.2013

    Figure Lengend Snippet: Cx50 mutant subunits differ in their ability to localize to the plasma membrane. Transiently transfected HeLa cells expressing WT Cx50 ( A ), V44E ( B ), D47N ( C ), and V79L ( D ) proteins were immunostained and examined by fluorescence microscopy. Merged images of Cy3 (red) staining of connexins and DAPI staining of cell nuclei (blue) were taken at ×40 magnification. D47N failed to correctly localize to cell membrane appositions, whereas V44E and V79L formed gap junction plaques at areas of cell-cell contact similar to WT Cx50.

    Article Snippet: Cells were stained with a polyclonal anti-Cx50 antibody followed by incubation with an anti-Cy3 goat anti-rabbit secondary antibody (Jackson ImmunoResearch).

    Techniques: Mutagenesis, Transfection, Expressing, Fluorescence, Microscopy, Staining

    Lhx6-EGFP is reliably expressed in fast-spiking perisomatic-targeting PV + cells. A , Confocal image stack from a horizontal section (50 μm) through the DG of a juvenile Lhx6-EGFP mouse. Note the high density of Lhx6-EGFP-positive somata at the gcl–hilus border (arrows). Inset, Confocal image demonstrating colocalization of EGFP and Lhx6 detected with a primary antibody and visualized using a secondary antibody conjugated to Cy3. Scale bar, 20 μm. B , Left, A single Lhx6-EGFP-positive neuron was filled with biocytin during whole-cell recording and visualized subsequently. The dense axonal arborizations in the gcl (arrows) identify this neuron as a perisomatic-targeting cell. The selected somatic area (red box) is shown on the right at higher magnification. Inset, Perisomatic-targeting cells had a typical fast-spiking non-adapting discharge pattern (600 pA, 1 s; V hold −70 mV). Right, Confocal image stack of the same cell stained against parvalbumin, a characteristic marker for perisomatic-targeting cells. Scale bar, 10 μm. ml, Molecular layer.

    Journal: The Journal of Neuroscience

    Article Title: Recruitment of Early Postnatal Parvalbumin-Positive Hippocampal Interneurons by GABAergic Excitation

    doi: 10.1523/JNEUROSCI.4125-09.2010

    Figure Lengend Snippet: Lhx6-EGFP is reliably expressed in fast-spiking perisomatic-targeting PV + cells. A , Confocal image stack from a horizontal section (50 μm) through the DG of a juvenile Lhx6-EGFP mouse. Note the high density of Lhx6-EGFP-positive somata at the gcl–hilus border (arrows). Inset, Confocal image demonstrating colocalization of EGFP and Lhx6 detected with a primary antibody and visualized using a secondary antibody conjugated to Cy3. Scale bar, 20 μm. B , Left, A single Lhx6-EGFP-positive neuron was filled with biocytin during whole-cell recording and visualized subsequently. The dense axonal arborizations in the gcl (arrows) identify this neuron as a perisomatic-targeting cell. The selected somatic area (red box) is shown on the right at higher magnification. Inset, Perisomatic-targeting cells had a typical fast-spiking non-adapting discharge pattern (600 pA, 1 s; V hold −70 mV). Right, Confocal image stack of the same cell stained against parvalbumin, a characteristic marker for perisomatic-targeting cells. Scale bar, 10 μm. ml, Molecular layer.

    Article Snippet: PV-labeling was done with a polyclonal rabbit-anti-PV primary (Swant, 1:1000) and goat-anti-rabbit Cy3 secondary antibody (Jackson ImmunoResearch, 1:1000).

    Techniques: Staining, Marker