cy3 conjugated goat anti rabbit igg  (Jackson Immuno)

 
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    Name:
    Cy 3 AffiniPure Goat Anti Rabbit IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    111-165-003
    Price:
    117.0
    Category:
    Whole IgG Affinity Purified Antibodies
    Conjugate:
    Cyanine Cy 3
    Size:
    2 0 mg
    Format:
    Whole IgG
    Host:
    Goat
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    Structured Review

    Jackson Immuno cy3 conjugated goat anti rabbit igg
    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit <t>IgG</t> (green) and mono-clonal rat antibody against mouse CD68 followed by <t>Cy3-con-jugated</t> anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/cy3 conjugated goat anti rabbit igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated goat anti rabbit igg - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "TLR4 mediates LPS-induced HO-1 expression in mouse liver: Role of TNF-α and IL-1β"

    Article Title: TLR4 mediates LPS-induced HO-1 expression in mouse liver: Role of TNF-α and IL-1β

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.v9.i8.1799

    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.
    Figure Legend Snippet: Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.

    Techniques Used: Staining

    2) Product Images from "Cerebellar neurons possess a vesicular compartment structurally and functionally similar to Glut4-storage vesicles from peripheral insulin-sensitive tissues"

    Article Title: Cerebellar neurons possess a vesicular compartment structurally and functionally similar to Glut4-storage vesicles from peripheral insulin-sensitive tissues

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    doi: 10.1523/JNEUROSCI.0858-09.2009

    Glut4 is translocated to the cell surface of cerebellar neurons in response to insulin stimulation A: Primary cultures of cerebellum neurons were serum-starved for 2h and treated or not treated with 100 nM insulin for 15 min. Top panels: cells were stained with the polyclonal antibody MC2A against Glut4 followed by Cy3-conjugated donkey anti-rabbit IgG. Bottom panels: phase-contrast images. B: Primary cultures of cerebellar neurons were serum starved for 2 h and treated as indicated with Indinavir (100 nM) for 4 hrs, Wortmannin (100 nM) and Cytochalasin B (5 μM) for 30 min, and Insulin (100 nM) for 15 min. Glucose uptake was measured in cultured cells in triplicate. A representative result of three independent experiments is shown.
    Figure Legend Snippet: Glut4 is translocated to the cell surface of cerebellar neurons in response to insulin stimulation A: Primary cultures of cerebellum neurons were serum-starved for 2h and treated or not treated with 100 nM insulin for 15 min. Top panels: cells were stained with the polyclonal antibody MC2A against Glut4 followed by Cy3-conjugated donkey anti-rabbit IgG. Bottom panels: phase-contrast images. B: Primary cultures of cerebellar neurons were serum starved for 2 h and treated as indicated with Indinavir (100 nM) for 4 hrs, Wortmannin (100 nM) and Cytochalasin B (5 μM) for 30 min, and Insulin (100 nM) for 15 min. Glucose uptake was measured in cultured cells in triplicate. A representative result of three independent experiments is shown.

    Techniques Used: Staining, Cell Culture

    3) Product Images from "A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity"

    Article Title: A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0083077

    Immunostaining of A. fumigatus by anti-AFL polyclonal IgG. (A) A. fumigatus conidia lysate was loaded onto fucose-modified agarose resin and unbound proteins (line U), weakly bound proteins (W1-W3) and strongly interacting proteins (S) were analyzed on 12% SDS-PAGE with silver staining. Recombinant AFL (rAFL) was used for comparison. (B) Immunoblotting of gel A stained by polyclonal anti-AFL antibodies. Only recombinant AFL and one band of strongly interacting proteins identified as AFL (boxed) were stained. (C-G) Cultures from solid medium were harvested and immunolabeled. Images of conidiophores and several conidia clusters in visible light (C), fluorescence in the presence of anti-AFL polyclonal antibodies with Cy3-conjugated goat anti-rabbit IgG as secondary antibody (D) and a superposition of the two images (E). P. pastoris cells treated in the same way were used as a negative control - in visible light (F) and fluorescence (G).
    Figure Legend Snippet: Immunostaining of A. fumigatus by anti-AFL polyclonal IgG. (A) A. fumigatus conidia lysate was loaded onto fucose-modified agarose resin and unbound proteins (line U), weakly bound proteins (W1-W3) and strongly interacting proteins (S) were analyzed on 12% SDS-PAGE with silver staining. Recombinant AFL (rAFL) was used for comparison. (B) Immunoblotting of gel A stained by polyclonal anti-AFL antibodies. Only recombinant AFL and one band of strongly interacting proteins identified as AFL (boxed) were stained. (C-G) Cultures from solid medium were harvested and immunolabeled. Images of conidiophores and several conidia clusters in visible light (C), fluorescence in the presence of anti-AFL polyclonal antibodies with Cy3-conjugated goat anti-rabbit IgG as secondary antibody (D) and a superposition of the two images (E). P. pastoris cells treated in the same way were used as a negative control - in visible light (F) and fluorescence (G).

    Techniques Used: Immunostaining, Modification, SDS Page, Silver Staining, Recombinant, Staining, Immunolabeling, Fluorescence, Negative Control

    4) Product Images from "PPAR? mediates the hypolipidemic action of fibrates by antagonizing FoxO1"

    Article Title: PPAR? mediates the hypolipidemic action of fibrates by antagonizing FoxO1

    Journal: American journal of physiology. Endocrinology and metabolism

    doi: 10.1152/ajpendo.00157.2006

    FoxO1 immunohistochemistry. Liver tissue of hamsters fed regular chow ( A , B, and C ), high-fructose diet ( D, E, and F ), and high-fructose diet plus fenofibrate treatment ( G , H , and I ) was embedded with the Histoprep tissue embedding media. Frozen sections (8 μm) were cut and incubated with rabbit anti-FoxO1 antibody ( A , D , and G ), followed by immunostaining with Cy3-conjugated goat anti-rabbit IgG (red). The nuclei of hepatocytes ( B, E, and H ) were stained with the TO-PRO-3 dye (blue). Bar =10 μm.
    Figure Legend Snippet: FoxO1 immunohistochemistry. Liver tissue of hamsters fed regular chow ( A , B, and C ), high-fructose diet ( D, E, and F ), and high-fructose diet plus fenofibrate treatment ( G , H , and I ) was embedded with the Histoprep tissue embedding media. Frozen sections (8 μm) were cut and incubated with rabbit anti-FoxO1 antibody ( A , D , and G ), followed by immunostaining with Cy3-conjugated goat anti-rabbit IgG (red). The nuclei of hepatocytes ( B, E, and H ) were stained with the TO-PRO-3 dye (blue). Bar =10 μm.

    Techniques Used: Immunohistochemistry, Incubation, Immunostaining, Staining

    5) Product Images from "Recombinant pICln Forms Highly Cation-selective Channels when Reconstituted into Artificial and Biological Membranes "

    Article Title: Recombinant pICln Forms Highly Cation-selective Channels when Reconstituted into Artificial and Biological Membranes

    Journal: The Journal of General Physiology

    doi:

    pI Cln added to the extracellular medium inserts spontaneously into the plasma membrane of Sf9 cells. Cells were exposed to 100 μg/ml pI Cln for 60 min. After washing and fixation, pI Cln was localized by immunostaining. Autofluorescence images (e.g., C and D ) were acquired to allow visualization of cells. ( A and B ) Examples of pI Cln -treated cells stained with anti–pI Cln polyclonal antisera and CY3-conjugated goat anti–rabbit IgG. Plasma membrane ( arrows ) shows intense staining for pI Cln . ( C ) pI Cln -treated cells incubated with preimmune serum show no staining. ( D ) Endogenous pI Cln is not detected in control Sf9 cells stained with anti–pI Cln antiserum.
    Figure Legend Snippet: pI Cln added to the extracellular medium inserts spontaneously into the plasma membrane of Sf9 cells. Cells were exposed to 100 μg/ml pI Cln for 60 min. After washing and fixation, pI Cln was localized by immunostaining. Autofluorescence images (e.g., C and D ) were acquired to allow visualization of cells. ( A and B ) Examples of pI Cln -treated cells stained with anti–pI Cln polyclonal antisera and CY3-conjugated goat anti–rabbit IgG. Plasma membrane ( arrows ) shows intense staining for pI Cln . ( C ) pI Cln -treated cells incubated with preimmune serum show no staining. ( D ) Endogenous pI Cln is not detected in control Sf9 cells stained with anti–pI Cln antiserum.

    Techniques Used: Immunostaining, Staining, Incubation

    6) Product Images from "New Molecular Mechanism for Ullrich Congenital Muscular Dystrophy: A Heterozygous In-Frame Deletion in the COL6A1 Gene Causes a Severe Phenotype"

    Article Title: New Molecular Mechanism for Ullrich Congenital Muscular Dystrophy: A Heterozygous In-Frame Deletion in the COL6A1 Gene Causes a Severe Phenotype

    Journal: American Journal of Human Genetics

    doi:

    Deposition of collagen VI microfibrils in the extracellular matrix of dermal fibroblasts. Fibroblasts from an unaffected control individual ( A and B ), patient UC-1 ( C and D ), and patient UC-4 ( E and F ) were grown in the presence of 50 μg/ml L-ascorbic acid phosphate for 4 d postconfluency and were stained with the α1(VI) collagen-specific antibody. The antibody reaction was detected with Cy3-conjugated goat anti-rabbit IgG ( red ), and cells were counterstained with DAPI ( blue ) to visualize nuclei. Original magnifications ×20 ( A, C, and E ) and ×40 ( B, D, and F ).
    Figure Legend Snippet: Deposition of collagen VI microfibrils in the extracellular matrix of dermal fibroblasts. Fibroblasts from an unaffected control individual ( A and B ), patient UC-1 ( C and D ), and patient UC-4 ( E and F ) were grown in the presence of 50 μg/ml L-ascorbic acid phosphate for 4 d postconfluency and were stained with the α1(VI) collagen-specific antibody. The antibody reaction was detected with Cy3-conjugated goat anti-rabbit IgG ( red ), and cells were counterstained with DAPI ( blue ) to visualize nuclei. Original magnifications ×20 ( A, C, and E ) and ×40 ( B, D, and F ).

    Techniques Used: Staining

    7) Product Images from "A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity"

    Article Title: A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0083077

    Immunostaining of A. fumigatus by anti-AFL polyclonal IgG. (A) A. fumigatus conidia lysate was loaded onto fucose-modified agarose resin and unbound proteins (line U), weakly bound proteins (W1-W3) and strongly interacting proteins (S) were analyzed on 12% SDS-PAGE with silver staining. Recombinant AFL (rAFL) was used for comparison. (B) Immunoblotting of gel A stained by polyclonal anti-AFL antibodies. Only recombinant AFL and one band of strongly interacting proteins identified as AFL (boxed) were stained. (C-G) Cultures from solid medium were harvested and immunolabeled. Images of conidiophores and several conidia clusters in visible light (C), fluorescence in the presence of anti-AFL polyclonal antibodies with Cy3-conjugated goat anti-rabbit IgG as secondary antibody (D) and a superposition of the two images (E). P. pastoris cells treated in the same way were used as a negative control - in visible light (F) and fluorescence (G).
    Figure Legend Snippet: Immunostaining of A. fumigatus by anti-AFL polyclonal IgG. (A) A. fumigatus conidia lysate was loaded onto fucose-modified agarose resin and unbound proteins (line U), weakly bound proteins (W1-W3) and strongly interacting proteins (S) were analyzed on 12% SDS-PAGE with silver staining. Recombinant AFL (rAFL) was used for comparison. (B) Immunoblotting of gel A stained by polyclonal anti-AFL antibodies. Only recombinant AFL and one band of strongly interacting proteins identified as AFL (boxed) were stained. (C-G) Cultures from solid medium were harvested and immunolabeled. Images of conidiophores and several conidia clusters in visible light (C), fluorescence in the presence of anti-AFL polyclonal antibodies with Cy3-conjugated goat anti-rabbit IgG as secondary antibody (D) and a superposition of the two images (E). P. pastoris cells treated in the same way were used as a negative control - in visible light (F) and fluorescence (G).

    Techniques Used: Immunostaining, Modification, SDS Page, Silver Staining, Recombinant, Staining, Immunolabeling, Fluorescence, Negative Control

    8) Product Images from "Blockade of TGF-? inhibits mammary tumor cell viability, migration, and metastases"

    Article Title: Blockade of TGF-? inhibits mammary tumor cell viability, migration, and metastases

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI15234

    Tumor morphology, rate of apoptosis, and Akt signaling are altered by treatment with Fc:TβRII. ( a ) MMTV/PyV mT mice treated with Fc:TβRII or normal mouse IgG were examined twice weekly from 21 to 110 days of age. The initial observation of tumor onset is represented. ( b ) Histologic sections of tumors from 35- or 70-day-old transgenic or wild-type mice. Arrowheads indicate dilated ducts filled with secretory products. ( c ) TUNEL analysis (panels 1–4) of tumors harvested from 35- or 70-day-old mice treated with Fc:TβRII or IgG. n = 6 per condition. Quantification of percentage of apoptotic nuclei (bottom right corner of each panel) was calculated using the following equation: (number of TUNEL-positive nuclei in ×400 field) / (number of total nuclei in ×400 field). BrdU incorporation analysis (panels 5–8) of tumors harvested from 70-day-old MMTV/PyV mT or wild-type mice. Arrowheads in panels 7 and 8 indicate BrdU-positive nuclei. Quantification of percentage of BrdU-positive nuclei (bottom right corner of each panel) was calculated using the following equation: (number of BrdU-positive nuclei in ×400 field) / (number of total nuclei in ×400 field). * P = 0.15. Scale bars = 25 μm. ( d ) Tumor extracts harvested from 110-day-old transgenic mice were subjected to Western blot analysis using Ab’s against the mT Ag, Shc, p85, Akt, p -Akt, FKHRL1, and p -FKHRL. ( e ) PMTCs were incubated with or without 20 nM Fc:TβRII for 6 hours and stained with a FKHRL1 Ab followed by staining with Cy3-conjugated anti-rabbit Ab. Nuclei were counterstained with DAPI.
    Figure Legend Snippet: Tumor morphology, rate of apoptosis, and Akt signaling are altered by treatment with Fc:TβRII. ( a ) MMTV/PyV mT mice treated with Fc:TβRII or normal mouse IgG were examined twice weekly from 21 to 110 days of age. The initial observation of tumor onset is represented. ( b ) Histologic sections of tumors from 35- or 70-day-old transgenic or wild-type mice. Arrowheads indicate dilated ducts filled with secretory products. ( c ) TUNEL analysis (panels 1–4) of tumors harvested from 35- or 70-day-old mice treated with Fc:TβRII or IgG. n = 6 per condition. Quantification of percentage of apoptotic nuclei (bottom right corner of each panel) was calculated using the following equation: (number of TUNEL-positive nuclei in ×400 field) / (number of total nuclei in ×400 field). BrdU incorporation analysis (panels 5–8) of tumors harvested from 70-day-old MMTV/PyV mT or wild-type mice. Arrowheads in panels 7 and 8 indicate BrdU-positive nuclei. Quantification of percentage of BrdU-positive nuclei (bottom right corner of each panel) was calculated using the following equation: (number of BrdU-positive nuclei in ×400 field) / (number of total nuclei in ×400 field). * P = 0.15. Scale bars = 25 μm. ( d ) Tumor extracts harvested from 110-day-old transgenic mice were subjected to Western blot analysis using Ab’s against the mT Ag, Shc, p85, Akt, p -Akt, FKHRL1, and p -FKHRL. ( e ) PMTCs were incubated with or without 20 nM Fc:TβRII for 6 hours and stained with a FKHRL1 Ab followed by staining with Cy3-conjugated anti-rabbit Ab. Nuclei were counterstained with DAPI.

    Techniques Used: Mouse Assay, Transgenic Assay, TUNEL Assay, BrdU Incorporation Assay, Western Blot, Incubation, Staining

    9) Product Images from "Neurotrophins regulate agrin-induced postsynaptic differentiation"

    Article Title: Neurotrophins regulate agrin-induced postsynaptic differentiation

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    BDNF/NT-4 inhibition of AChR clustering is mediated by TrkB receptors. ( A ) The mRNA-encoding full-length TrkB is present in cultured chicken myotubes. Nested PCR performed on a clone of full-length chicken TrkB produced a product of approximately 570 bp (lane 1). Nested PCR performed on two concentrations of a cDNA library made from chicken myotubes (lanes 3 and 4) or reverse transcription–PCR on RNA isolated from chicken myotubes in culture (lane 6) also produced an identical sized product. Lanes 2 and 5 had water substituted for DNA in the nested PCR reaction to control for contamination. (Bar = 5 μm.) ( B ) TrkB expression in myotubes. Cultured myotubes were fixed and labeled with either anti-TrkB antibody or an irrelevant IgG followed by an anti-rabbit-IgG secondary antibody conjugated to Cy3. TrkB immunoreactivity is distributed in a finely punctate pattern along the surfaces of the myotube. ( C ). Activation of TrkB inhibited agrin-induced clustering ( n = 4; ∗, P
    Figure Legend Snippet: BDNF/NT-4 inhibition of AChR clustering is mediated by TrkB receptors. ( A ) The mRNA-encoding full-length TrkB is present in cultured chicken myotubes. Nested PCR performed on a clone of full-length chicken TrkB produced a product of approximately 570 bp (lane 1). Nested PCR performed on two concentrations of a cDNA library made from chicken myotubes (lanes 3 and 4) or reverse transcription–PCR on RNA isolated from chicken myotubes in culture (lane 6) also produced an identical sized product. Lanes 2 and 5 had water substituted for DNA in the nested PCR reaction to control for contamination. (Bar = 5 μm.) ( B ) TrkB expression in myotubes. Cultured myotubes were fixed and labeled with either anti-TrkB antibody or an irrelevant IgG followed by an anti-rabbit-IgG secondary antibody conjugated to Cy3. TrkB immunoreactivity is distributed in a finely punctate pattern along the surfaces of the myotube. ( C ). Activation of TrkB inhibited agrin-induced clustering ( n = 4; ∗, P

    Techniques Used: Inhibition, Cell Culture, Nested PCR, Produced, cDNA Library Assay, Polymerase Chain Reaction, Isolation, Expressing, Labeling, Activation Assay

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    Article Title: VCP maintains nuclear size by regulating the DNA damage-associated MDC1–p53–autophagy axis in Drosophila
    Article Snippet: Alexa Fluor® 488, Alexa Fluor® 647, Cy3, and Cy5 conjugated secondary antibodies: Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H + L) (115-545-146, Jackson ImmunoResearch Laboratories), Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H + L) (111-545-144, Jackson ImmunoResearch Laboratories), Alexa Fluor® 647 AffiniPure Goat Anti-Mouse IgG (H + L) (115-605-146, Jackson ImmunoResearch Laboratories), Cy™3 AffiniPure Goat Anti-Mouse IgG (H + L) (115-165-146, Jackson ImmunoResearch Laboratories), Cy™5 AffiniPure Goat Anti-Mouse IgG (H + L) (115-175-146, Jackson ImmunoResearch Laboratories), Cy™3 AffiniPure Goat Anti-Rabbit IgG (H + L) (111-165-144, Jackson ImmunoResearch Laboratories), Cy™5 AffiniPure Goat Anti-Rabbit IgG (H + L) (111-175-144, Jackson ImmunoResearch Laboratories) were used at 1:100 dilutions.

    Incubation:

    Article Title: P32-specific CAR T cells with dual antitumor and antiangiogenic therapeutic potential in gliomas
    Article Snippet: .. Floating sections were incubated overnight at 4 °C with the following antibodies: rabbit anti-p32 polyclonal antibody (gift from Prof. Tambet Teesalu, stock 4.5 mg/ml, Dilution: 1:100), rabbit anti-vWF (Abcam, Cat. No. ab6994, Lot GR3180938-1, Dilution 1:100), followed by goat anti-rabbit-AlexaFluor488 (Abcam, Cat No. ab150077, dilution 1:500), or goat anti-rabbit Cy3 (Jackson ImmunoResearch, Cat No 111-165-144, Lot 123834, dilution 1:400). ..

    Article Title: A female-specific role for Calcitonin Gene-Related Peptide (CGRP) in rodent pain models
    Article Snippet: .. After washing in PBS, the sections were incubated for 2 h at room temperature in a solution containing a mixture of goat-Cy3 anti-rabbit (1:500, Jackson ImmunoResearch Laboratories, Cat. #111–165–144), goat anti-chicken Alexa 647 (1:500, Thermo Fisher Scientific Cat. #A-21449), goat anti-mouse Alexa 405 (1:500, Thermo Fisher Scientific Cat. #A-31553) and IB4 conjugated with Alexa 488 (1:500, Thermo Fisher Scientific Cat. #I21411) diluted in PBST (pH 7.4) containing 10% normal goat serum. ..

    Article Title: Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis
    Article Snippet: .. ImmunofluorescenceThe TNFα-stimulated chondrocytes were washed twice with 1X PBS and fixed with 10% formalin at RT for 15 min, followed by permeabilization with 1X PBS containing 0.1% Triton X-100 and 1% BSA (cat. no. BSA-BSH-1XG; Rocky Mountain Biologicals, Inc.) at RT for 1 h, incubation with a primary antibody at 4˚C overnight, washing with 1X PBS and incubation with Cy3-conjugated anti-rabbit antibody (cat. no. 111-165-144; Jackson Immunoresearch) or Alexa 488-conjugated anti-mouse antibody (cat. no. A-11001; Invitrogen) for 1 h. All primary and secondary antibodies were used at 1:100 dilution. ..

    Article Title: Metabolic Pathways Involved in Formation of Spontaneous and Lipopolysaccharide-Induced Neutrophil Extracellular Traps (NETs) Differ in Obesity and Systemic Inflammation
    Article Snippet: .. The slides were then washed two times in PBS (5 min) and incubated with Cy3-conjugated goat anti-rabbit IgG (H+L) antibody (diluted 1:300 in PBS/1% BSA; Jackson Immunoresearch, Ely, UK) for 1 h at RT. ..

    Article Title: Sensorineural Hearing Loss and Mitochondrial Apoptosis of Cochlear Spiral Ganglion Neurons in Fibroblast Growth Factor 13 Knockout Mice
    Article Snippet: .. The secondary antibodies were Fluorescein (FITC)-conjugated AffiniPure goat-anti-mouse IgG (1:300; 115-095-166, Jackson ImmunoResearch, United States) and cyTM 3-conjugated AffiniPure goat-anti-rabbit IgG (1:200; 111-165-144, Jackson Immunoresearch, United States) diluted in the solution (1% BSA and 0.1% Triton X-100 for sections; 1% BSA, 0.1% Triton X-100 and 5% goat serum for whole mounts) at RT for 90 min. As controls for specificity, the primary antibody was co-incubated with the peptide used for immunization or samples were incubated with secondary antibody only. ..

    Article Title: Decorin—An Antagonist of TGF-β in Astrocytes of the Optic Nerve
    Article Snippet: .. Afterwards, tissue sections were washed three times with 0.1 M phosphate buffer followed by incubation for 1 h at room temperature with Cy3™ goat anti-rabbit (1:2000, Jackson Immuno Research Europe Ltd., Suffolk, UK) and/or Alexa Flour 488 goat anti-chicken (1:1000, ThermoFisher Scientific, Waltham, Massachusetts, USA). ..

    Staining:

    Article Title: Thrombocytopenia and splenic platelet directed immune responses after intravenous ChAdOx1 nCov-19 administration
    Article Snippet: .. Secondary antibodies and nucleic acid stain included FITC goat anti mouse (Thermofisher, Cat. No. A11029) and Cy3 goat anti rabbit (Jackson Immunoresearch, Cat. No. 111-165-003) and Hoechst33342. ..

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  • 99
    Jackson Immuno cy3 conjugated goat anti rabbit igg
    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit <t>IgG</t> (green) and mono-clonal rat antibody against mouse CD68 followed by <t>Cy3-con-jugated</t> anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.
    Cy3 Conjugated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated goat anti rabbit igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated goat anti rabbit igg - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    86
    Jackson Immuno goat anti rabbit cy3 conjugated affinipure igg
    Immunofluorescent assessment of SFRP2 expression in the endobronchial biopsies from large airway epithelium. Endobronchial biopsies from large airway of healthy nonsmoker, healthy smokers and smokers with COPD were stained with anti-SFRP2 followed by a <t>Cy3</t> conjugated secondary antibody (shown in red). Nuclei were stained with DAPI (shown in blue). A–D . Healthy nonsmokers. A . <t>IgG</t> control; B–D . Examples of anti-SFRP2. E–H . Healthy smokers. E . IgG control; F–H . Examples of anti-SFRP2. I–L . Smokers with COPD. I . IgG Control, J–L . Examples of anti-SFRP2. Bar = 10 µm.
    Goat Anti Rabbit Cy3 Conjugated Affinipure Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit cy3 conjugated affinipure igg/product/Jackson Immuno
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit cy3 conjugated affinipure igg - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    99
    Jackson Immuno goat anti rabbit secondary antibody
    Characterization of the calcineurin regulatory subunit in WT and Ppp3r2 knockout mice. A) Genotyping of knockout mice by PCR using primers designed to the detect the presence of knock out allele (containing LacZ reporter gene), as described in the materials and methods section, shows absence of Ppp3r2 . Figure in the right panel is the western blot analysis of sperm cell extracts from KO and wild type mice using specific <t>antibodies</t> shows the absence of PPP3R2 in KO mice. B) Immunostaining of adult testis sections shows absence of PPP32 in knockout mice while it is present within the seminiferous tubules of the testis of wild type mice; green arrow indicates the absence of PPP3R2 in Sertoli cell. Hoechst dye was used to identify nuclei. C) Sperm permeabilized with 0.5% Triton X-100 and fixed with paraformaldehyde and stained with <t>rabbit</t> PPP3R2 antibodies followed by Alexa fluor-488 labelled <t>secondary</t> antibodies. The merged figures in the left of each panel shows localization of the protein mainly in the midpiece and dimly in the principal piece of sperm from wild type sperm while there is no detectable signal in sperm from knock out mice.
    Goat Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit secondary antibody/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit secondary antibody - by Bioz Stars, 2021-09
    99/100 stars
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    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: TLR4 mediates LPS-induced HO-1 expression in mouse liver: Role of TNF-α and IL-1β

    doi: 10.3748/wjg.v9.i8.1799

    Figure Lengend Snippet: Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.

    Article Snippet: Rat IgG and Cy3-conjugated goat anti-rabbit IgG were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).

    Techniques: Staining

    Glut4 is translocated to the cell surface of cerebellar neurons in response to insulin stimulation A: Primary cultures of cerebellum neurons were serum-starved for 2h and treated or not treated with 100 nM insulin for 15 min. Top panels: cells were stained with the polyclonal antibody MC2A against Glut4 followed by Cy3-conjugated donkey anti-rabbit IgG. Bottom panels: phase-contrast images. B: Primary cultures of cerebellar neurons were serum starved for 2 h and treated as indicated with Indinavir (100 nM) for 4 hrs, Wortmannin (100 nM) and Cytochalasin B (5 μM) for 30 min, and Insulin (100 nM) for 15 min. Glucose uptake was measured in cultured cells in triplicate. A representative result of three independent experiments is shown.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Cerebellar neurons possess a vesicular compartment structurally and functionally similar to Glut4-storage vesicles from peripheral insulin-sensitive tissues

    doi: 10.1523/JNEUROSCI.0858-09.2009

    Figure Lengend Snippet: Glut4 is translocated to the cell surface of cerebellar neurons in response to insulin stimulation A: Primary cultures of cerebellum neurons were serum-starved for 2h and treated or not treated with 100 nM insulin for 15 min. Top panels: cells were stained with the polyclonal antibody MC2A against Glut4 followed by Cy3-conjugated donkey anti-rabbit IgG. Bottom panels: phase-contrast images. B: Primary cultures of cerebellar neurons were serum starved for 2 h and treated as indicated with Indinavir (100 nM) for 4 hrs, Wortmannin (100 nM) and Cytochalasin B (5 μM) for 30 min, and Insulin (100 nM) for 15 min. Glucose uptake was measured in cultured cells in triplicate. A representative result of three independent experiments is shown.

    Article Snippet: Sections were blocked with MOM mouse IgG blocking reagent (Vector Laboratories), washed with Gadenza buffer (Vector Laboratories) and stained with polyclonal anti-Glut4 antibody MC2A followed by Cy3-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch).

    Techniques: Staining, Cell Culture

    Immunofluorescent assessment of SFRP2 expression in the endobronchial biopsies from large airway epithelium. Endobronchial biopsies from large airway of healthy nonsmoker, healthy smokers and smokers with COPD were stained with anti-SFRP2 followed by a Cy3 conjugated secondary antibody (shown in red). Nuclei were stained with DAPI (shown in blue). A–D . Healthy nonsmokers. A . IgG control; B–D . Examples of anti-SFRP2. E–H . Healthy smokers. E . IgG control; F–H . Examples of anti-SFRP2. I–L . Smokers with COPD. I . IgG Control, J–L . Examples of anti-SFRP2. Bar = 10 µm.

    Journal: PLoS ONE

    Article Title: Down-Regulation of the Canonical Wnt ?-Catenin Pathway in the Airway Epithelium of Healthy Smokers and Smokers with COPD

    doi: 10.1371/journal.pone.0014793

    Figure Lengend Snippet: Immunofluorescent assessment of SFRP2 expression in the endobronchial biopsies from large airway epithelium. Endobronchial biopsies from large airway of healthy nonsmoker, healthy smokers and smokers with COPD were stained with anti-SFRP2 followed by a Cy3 conjugated secondary antibody (shown in red). Nuclei were stained with DAPI (shown in blue). A–D . Healthy nonsmokers. A . IgG control; B–D . Examples of anti-SFRP2. E–H . Healthy smokers. E . IgG control; F–H . Examples of anti-SFRP2. I–L . Smokers with COPD. I . IgG Control, J–L . Examples of anti-SFRP2. Bar = 10 µm.

    Article Snippet: Goat anti-rabbit Cy3 conjugated AffiniPure IgG (Jackson ImmunoResearch) at 1∶50 dilution was used as a secondary antibody for SFRP2.

    Techniques: Expressing, Staining

    Immunofluorescent assessment of SFRP2 expression in cytospin preparations of brushed small airway epithelium. Small airway epithelial cell cytopreparations of healthy nonsmokers, healthy smokers and smokers with COPD were stained with anti-SFRP2 followed by a Cy3 conjugated secondary antibody (shown in red). Nuclei were stained with DAPI (shown in blue) A–D . Healthy nonsmokers. A . IgG control; B–D . Examples of anti-SFRP2. E–H . Healthy smokers. E . IgG control; F–H . Examples of anti-SFRP2. I–L . Smokers with COPD; I . IgG Control. J–L . Examples of anti-SFRP2. Bar = 10 µm.

    Journal: PLoS ONE

    Article Title: Down-Regulation of the Canonical Wnt ?-Catenin Pathway in the Airway Epithelium of Healthy Smokers and Smokers with COPD

    doi: 10.1371/journal.pone.0014793

    Figure Lengend Snippet: Immunofluorescent assessment of SFRP2 expression in cytospin preparations of brushed small airway epithelium. Small airway epithelial cell cytopreparations of healthy nonsmokers, healthy smokers and smokers with COPD were stained with anti-SFRP2 followed by a Cy3 conjugated secondary antibody (shown in red). Nuclei were stained with DAPI (shown in blue) A–D . Healthy nonsmokers. A . IgG control; B–D . Examples of anti-SFRP2. E–H . Healthy smokers. E . IgG control; F–H . Examples of anti-SFRP2. I–L . Smokers with COPD; I . IgG Control. J–L . Examples of anti-SFRP2. Bar = 10 µm.

    Article Snippet: Goat anti-rabbit Cy3 conjugated AffiniPure IgG (Jackson ImmunoResearch) at 1∶50 dilution was used as a secondary antibody for SFRP2.

    Techniques: Expressing, Staining

    Characterization of the calcineurin regulatory subunit in WT and Ppp3r2 knockout mice. A) Genotyping of knockout mice by PCR using primers designed to the detect the presence of knock out allele (containing LacZ reporter gene), as described in the materials and methods section, shows absence of Ppp3r2 . Figure in the right panel is the western blot analysis of sperm cell extracts from KO and wild type mice using specific antibodies shows the absence of PPP3R2 in KO mice. B) Immunostaining of adult testis sections shows absence of PPP32 in knockout mice while it is present within the seminiferous tubules of the testis of wild type mice; green arrow indicates the absence of PPP3R2 in Sertoli cell. Hoechst dye was used to identify nuclei. C) Sperm permeabilized with 0.5% Triton X-100 and fixed with paraformaldehyde and stained with rabbit PPP3R2 antibodies followed by Alexa fluor-488 labelled secondary antibodies. The merged figures in the left of each panel shows localization of the protein mainly in the midpiece and dimly in the principal piece of sperm from wild type sperm while there is no detectable signal in sperm from knock out mice.

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs

    doi: 10.1096/fj.201902163R

    Figure Lengend Snippet: Characterization of the calcineurin regulatory subunit in WT and Ppp3r2 knockout mice. A) Genotyping of knockout mice by PCR using primers designed to the detect the presence of knock out allele (containing LacZ reporter gene), as described in the materials and methods section, shows absence of Ppp3r2 . Figure in the right panel is the western blot analysis of sperm cell extracts from KO and wild type mice using specific antibodies shows the absence of PPP3R2 in KO mice. B) Immunostaining of adult testis sections shows absence of PPP32 in knockout mice while it is present within the seminiferous tubules of the testis of wild type mice; green arrow indicates the absence of PPP3R2 in Sertoli cell. Hoechst dye was used to identify nuclei. C) Sperm permeabilized with 0.5% Triton X-100 and fixed with paraformaldehyde and stained with rabbit PPP3R2 antibodies followed by Alexa fluor-488 labelled secondary antibodies. The merged figures in the left of each panel shows localization of the protein mainly in the midpiece and dimly in the principal piece of sperm from wild type sperm while there is no detectable signal in sperm from knock out mice.

    Article Snippet: Slides were washed three times with 1X PBS and incubated with the goat anti-rabbit secondary antibody (1∶250) conjugated with Cy3 (Jackson Immunoresearch laboratories, West Grove PA) for 2 hours at room temperature.

    Techniques: Knock-Out, Mouse Assay, Polymerase Chain Reaction, Western Blot, Immunostaining, Staining

    Effect of ppp3r2 deletion on sperm ATP levels and distribution of monocarboxylate transporter protein complex. A) Wild type and knock out sperm were incubated in presence or absence of the indicated ener gy substrates for 1 hour and ATP levels were determined by a luciferase assay as described. ATP level in the ppp3r2 knockout spermatozoa incubated in presence of pyruvate did not increase as opposed to that of WT sperm population. B) Expression of MCT2 and its binding partner, basigin in calcineurin KO mouse sperm were significantly lower as seen in western blot of whole sperm lysate (upper panel) and immune-cytofluorescence (lower panel) developed by anti-mouse MCT2 and anti-goat EMMPRIN (basigin) antibodies. As loading control for the western blots, mouse monoclonal anti-β-actin antibodies were used; the calculated average band intensities (n=3) provided on top of the corresponding blots. For immunofluorescence, cyanine 3 and Alexa fluor488 conjugated secondary IgGs were used for MCT2 and basigin, respectively; Hoechst was used for DNA staining. Details of the protocol has been described in materials and methods. Merged picture is in the right-hand corner.

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs

    doi: 10.1096/fj.201902163R

    Figure Lengend Snippet: Effect of ppp3r2 deletion on sperm ATP levels and distribution of monocarboxylate transporter protein complex. A) Wild type and knock out sperm were incubated in presence or absence of the indicated ener gy substrates for 1 hour and ATP levels were determined by a luciferase assay as described. ATP level in the ppp3r2 knockout spermatozoa incubated in presence of pyruvate did not increase as opposed to that of WT sperm population. B) Expression of MCT2 and its binding partner, basigin in calcineurin KO mouse sperm were significantly lower as seen in western blot of whole sperm lysate (upper panel) and immune-cytofluorescence (lower panel) developed by anti-mouse MCT2 and anti-goat EMMPRIN (basigin) antibodies. As loading control for the western blots, mouse monoclonal anti-β-actin antibodies were used; the calculated average band intensities (n=3) provided on top of the corresponding blots. For immunofluorescence, cyanine 3 and Alexa fluor488 conjugated secondary IgGs were used for MCT2 and basigin, respectively; Hoechst was used for DNA staining. Details of the protocol has been described in materials and methods. Merged picture is in the right-hand corner.

    Article Snippet: Slides were washed three times with 1X PBS and incubated with the goat anti-rabbit secondary antibody (1∶250) conjugated with Cy3 (Jackson Immunoresearch laboratories, West Grove PA) for 2 hours at room temperature.

    Techniques: Knock-Out, Incubation, Luciferase, Expressing, Binding Assay, Western Blot, Immunofluorescence, Staining