cy3 conjugated anti rabbit igg  (Millipore)


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  • 95
    Name:
    Anti Goat IgG whole molecule Cy3 antibody
    Description:
    The product binds to all goat Igs
    Catalog Number:
    c2821
    Price:
    None
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    Structured Review

    Millipore cy3 conjugated anti rabbit igg
    Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit <t>IgG</t> and a secondary antibody labeled with <t>Cy3.</t> The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.
    The product binds to all goat Igs
    https://www.bioz.com/result/cy3 conjugated anti rabbit igg/product/Millipore
    Average 95 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated anti rabbit igg - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "Fibroblast Growth Factor Receptors (FGFRs) in Human Sperm: Expression, Functionality and Involvement in Motility Regulation"

    Article Title: Fibroblast Growth Factor Receptors (FGFRs) in Human Sperm: Expression, Functionality and Involvement in Motility Regulation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0127297

    Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit IgG and a secondary antibody labeled with Cy3. The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.
    Figure Legend Snippet: Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit IgG and a secondary antibody labeled with Cy3. The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.

    Techniques Used: Staining, Labeling

    Related Articles

    Flow Cytometry:

    Article Title: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells
    Article Snippet: .. Flow cytometry analysis for SSTR2 expression Following incubation with 1:100 dilution of anti-SSTR2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Catalog #, sc-11606) for 60 min, cells were cocultured with CY3-conjugated rabbit anti-goat IgG (Sigma, St Louis, MO, USA; Catalog #, C2821) for 60 min. After being washed and resuspended in PBS, cells were assessed with a flow cytometer (FCM, BD Biosciences, San Jose, CA, USA). .. Immunofluorescence assay for SSTR2 expression Paraformaldehyde-fixed MCF-7 cells were incubated with anti-SSTR2 polyclonal antibody overnight at 4 °C.

    Article Title: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells
    Article Snippet: .. Following incubation with 1:100 dilution of anti-SSTR2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Catalog #, sc-11606) for 60 min, cells were cocultured with CY3-conjugated rabbit anti-goat IgG (Sigma, St Louis, MO, USA; Catalog #, C2821) for 60 min. After being washed and resuspended in PBS, cells were assessed with a flow cytometer (FCM, BD Biosciences, San Jose, CA, USA). .. Paraformaldehyde-fixed MCF-7 cells were incubated with anti-SSTR2 polyclonal antibody overnight at 4 °C.

    Cytometry:

    Article Title: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells
    Article Snippet: .. Flow cytometry analysis for SSTR2 expression Following incubation with 1:100 dilution of anti-SSTR2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Catalog #, sc-11606) for 60 min, cells were cocultured with CY3-conjugated rabbit anti-goat IgG (Sigma, St Louis, MO, USA; Catalog #, C2821) for 60 min. After being washed and resuspended in PBS, cells were assessed with a flow cytometer (FCM, BD Biosciences, San Jose, CA, USA). .. Immunofluorescence assay for SSTR2 expression Paraformaldehyde-fixed MCF-7 cells were incubated with anti-SSTR2 polyclonal antibody overnight at 4 °C.

    Article Title: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells
    Article Snippet: .. Following incubation with 1:100 dilution of anti-SSTR2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Catalog #, sc-11606) for 60 min, cells were cocultured with CY3-conjugated rabbit anti-goat IgG (Sigma, St Louis, MO, USA; Catalog #, C2821) for 60 min. After being washed and resuspended in PBS, cells were assessed with a flow cytometer (FCM, BD Biosciences, San Jose, CA, USA). .. Paraformaldehyde-fixed MCF-7 cells were incubated with anti-SSTR2 polyclonal antibody overnight at 4 °C.

    Blocking Assay:

    Article Title: HAP1 Is Required for Endocytosis and Signalling of BDNF and Its Receptors in Neurons
    Article Snippet: .. Thereafter, the cells were then incubated with blocking buffer (3% bovine serum albumin (BSA) (Sigma-Aldrich, St Louis, MO, USA), TBS, 0.1% Triton X-100, 0.01% NaN3, pH 7.4) for 1 h at 25 °C, then incubated with an anti-BrdU monoclonal antibody (1:500, G3G4, DSHB, Iowa city, Iowa, USA), followed by a Cy3-conjugated goat anti-mouse IgG (1:200, C2821, Sigma-Aldrich, St Louis, MO, USA). .. The images were taken by CX40 fluorescence microscope (Olympus, VIC, Australia), and BrdU-positive cells were counted using ImageJ software (Research Service Branch; National Institute of Health, ).

    Incubation:

    Article Title: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells
    Article Snippet: .. Flow cytometry analysis for SSTR2 expression Following incubation with 1:100 dilution of anti-SSTR2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Catalog #, sc-11606) for 60 min, cells were cocultured with CY3-conjugated rabbit anti-goat IgG (Sigma, St Louis, MO, USA; Catalog #, C2821) for 60 min. After being washed and resuspended in PBS, cells were assessed with a flow cytometer (FCM, BD Biosciences, San Jose, CA, USA). .. Immunofluorescence assay for SSTR2 expression Paraformaldehyde-fixed MCF-7 cells were incubated with anti-SSTR2 polyclonal antibody overnight at 4 °C.

    Article Title: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells
    Article Snippet: .. Following incubation with 1:100 dilution of anti-SSTR2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Catalog #, sc-11606) for 60 min, cells were cocultured with CY3-conjugated rabbit anti-goat IgG (Sigma, St Louis, MO, USA; Catalog #, C2821) for 60 min. After being washed and resuspended in PBS, cells were assessed with a flow cytometer (FCM, BD Biosciences, San Jose, CA, USA). .. Paraformaldehyde-fixed MCF-7 cells were incubated with anti-SSTR2 polyclonal antibody overnight at 4 °C.

    Article Title: HAP1 Is Required for Endocytosis and Signalling of BDNF and Its Receptors in Neurons
    Article Snippet: .. Thereafter, the cells were then incubated with blocking buffer (3% bovine serum albumin (BSA) (Sigma-Aldrich, St Louis, MO, USA), TBS, 0.1% Triton X-100, 0.01% NaN3, pH 7.4) for 1 h at 25 °C, then incubated with an anti-BrdU monoclonal antibody (1:500, G3G4, DSHB, Iowa city, Iowa, USA), followed by a Cy3-conjugated goat anti-mouse IgG (1:200, C2821, Sigma-Aldrich, St Louis, MO, USA). .. The images were taken by CX40 fluorescence microscope (Olympus, VIC, Australia), and BrdU-positive cells were counted using ImageJ software (Research Service Branch; National Institute of Health, ).

    Article Title: Sipi soup inhibits cancer-associated fibroblast activation and the inflammatory process by downregulating long non-coding RNA HIPK1-AS
    Article Snippet: .. The cells were incubated with Cy3 Goat Anti-Rabbit IgG (cat. no. C2821, 1:200, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at 37°C for 1 h. The coverslips were stained with DAPI (1:2,000, SC-3598, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 min at room temperature and mounted on slides using anti-fade mounting medium (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). .. Immunofluorescence images were acquired using a fluorescence microscope (Nikon Eclipse 80i, Nikon Corporation, Tokyo, Japan).

    Expressing:

    Article Title: The antiproliferative effects of somatostatin receptor subtype 2 in breast cancer cells
    Article Snippet: .. Flow cytometry analysis for SSTR2 expression Following incubation with 1:100 dilution of anti-SSTR2 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Catalog #, sc-11606) for 60 min, cells were cocultured with CY3-conjugated rabbit anti-goat IgG (Sigma, St Louis, MO, USA; Catalog #, C2821) for 60 min. After being washed and resuspended in PBS, cells were assessed with a flow cytometer (FCM, BD Biosciences, San Jose, CA, USA). .. Immunofluorescence assay for SSTR2 expression Paraformaldehyde-fixed MCF-7 cells were incubated with anti-SSTR2 polyclonal antibody overnight at 4 °C.

    Staining:

    Article Title: Sipi soup inhibits cancer-associated fibroblast activation and the inflammatory process by downregulating long non-coding RNA HIPK1-AS
    Article Snippet: .. The cells were incubated with Cy3 Goat Anti-Rabbit IgG (cat. no. C2821, 1:200, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at 37°C for 1 h. The coverslips were stained with DAPI (1:2,000, SC-3598, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 min at room temperature and mounted on slides using anti-fade mounting medium (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). .. Immunofluorescence images were acquired using a fluorescence microscope (Nikon Eclipse 80i, Nikon Corporation, Tokyo, Japan).

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  • 95
    Millipore cy3 conjugated anti rabbit igg
    Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit <t>IgG</t> and a secondary antibody labeled with <t>Cy3.</t> The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.
    Cy3 Conjugated Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated anti rabbit igg/product/Millipore
    Average 95 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated anti rabbit igg - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    85
    Millipore cy3 conjugated donkey anti rabbit igg antibodies
    The expression of stem cell markers by Achilles tendon stem cells (ATSCs) in response to mouse treadmill running. TSCs were seeded in a 6-well plate, cultured and incubated with goat anti-mouse <t>nucleostemin/Cy3-conjugated</t> donkey anti-goat <t>IgG</t> antibodies ( A, D, G ), rabbit anti-mouse Oct-4/Cy3-conjugated donkey anti-rabbit IgG antibodies ( B, E, H ) or FITC-conjugated mouse anti-SSEA-1 ( C, F, I ). Compared to ATSCs from cage control mice ( A–C ), more cells in MTR ( D–F ) and ITR ( G–I ) groups increased the expression of stem cell markers nucleostemin (NS), Oct-4, and SSEA-1. It is apparent that the extent of the immunostaining on these stem cell markers is running-intensity-dependent. Positively stained cells were also counted to calculate percentage staining ( J ), which shows no significant difference between the groups. Bar: 100 µm.
    Cy3 Conjugated Donkey Anti Rabbit Igg Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated donkey anti rabbit igg antibodies/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated donkey anti rabbit igg antibodies - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit IgG and a secondary antibody labeled with Cy3. The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.

    Journal: PLoS ONE

    Article Title: Fibroblast Growth Factor Receptors (FGFRs) in Human Sperm: Expression, Functionality and Involvement in Motility Regulation

    doi: 10.1371/journal.pone.0127297

    Figure Lengend Snippet: Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit IgG and a secondary antibody labeled with Cy3. The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.

    Article Snippet: Other antibodies used were: anti pFGFR Tyr653/654 (#3476, Cell Signaling Technology, Inc., Beverley, MA, USA), anti pERK (sc-7383, Santa Cruz, and #4370, Cell Signaling), anti ERK (sc-94, Santa Cruz), anti pAkt Ser473 (sc-7985, Santa Cruz, and #4060, Cell Signaling), anti Akt (#4691, Cell Signaling), rabbit immunoglobulin G (IgG) (Sigma), horse-radish peroxidase (HRP)-conjugated anti-rabbit IgG (Sigma), Cy3-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse IgG (Chemicon-Millipore, Billerica, MA, USA) and anti-rabbit IgG (Sigma).

    Techniques: Staining, Labeling

    The expression of stem cell markers by Achilles tendon stem cells (ATSCs) in response to mouse treadmill running. TSCs were seeded in a 6-well plate, cultured and incubated with goat anti-mouse nucleostemin/Cy3-conjugated donkey anti-goat IgG antibodies ( A, D, G ), rabbit anti-mouse Oct-4/Cy3-conjugated donkey anti-rabbit IgG antibodies ( B, E, H ) or FITC-conjugated mouse anti-SSEA-1 ( C, F, I ). Compared to ATSCs from cage control mice ( A–C ), more cells in MTR ( D–F ) and ITR ( G–I ) groups increased the expression of stem cell markers nucleostemin (NS), Oct-4, and SSEA-1. It is apparent that the extent of the immunostaining on these stem cell markers is running-intensity-dependent. Positively stained cells were also counted to calculate percentage staining ( J ), which shows no significant difference between the groups. Bar: 100 µm.

    Journal: PLoS ONE

    Article Title: The Effects of Mechanical Loading on Tendons - An In Vivo and In Vitro Model Study

    doi: 10.1371/journal.pone.0071740

    Figure Lengend Snippet: The expression of stem cell markers by Achilles tendon stem cells (ATSCs) in response to mouse treadmill running. TSCs were seeded in a 6-well plate, cultured and incubated with goat anti-mouse nucleostemin/Cy3-conjugated donkey anti-goat IgG antibodies ( A, D, G ), rabbit anti-mouse Oct-4/Cy3-conjugated donkey anti-rabbit IgG antibodies ( B, E, H ) or FITC-conjugated mouse anti-SSEA-1 ( C, F, I ). Compared to ATSCs from cage control mice ( A–C ), more cells in MTR ( D–F ) and ITR ( G–I ) groups increased the expression of stem cell markers nucleostemin (NS), Oct-4, and SSEA-1. It is apparent that the extent of the immunostaining on these stem cell markers is running-intensity-dependent. Positively stained cells were also counted to calculate percentage staining ( J ), which shows no significant difference between the groups. Bar: 100 µm.

    Article Snippet: Cultures were washed three times with PBS, followed by application of Cy3-conjugated donkey anti-rabbit IgG antibodies (1∶500 for Oct-4, Millipore, Billerica, MA; Cat. #AC182C), or Cy3-conjugated Donkey anti-goat IgG antibodies (1∶500 for nucleostemin, Millipore, Billerica, MA; Cat. #AC180C) for 2 hrs at room temperature.

    Techniques: Expressing, Cell Culture, Incubation, Mouse Assay, Immunostaining, Staining

    The expression of stem cell markers by patellar tendon stem cells (PTSCs) in response to mouse treadmill running. TSCs were seeded in a 6-well plate, cultured and incubated with goat anti-mouse nucleostemin/Cy3-conjugated donkey anti-goat IgG antibodies ( A, D, G ), rabbit anti-mouse Oct-4/Cy3-conjugated donkey anti-rabbit IgG antibodies ( B, E, H ) or FITC-conjugated mouse anti-SSEA-1 ( C, F, I ). Compared to PTSCs from cage control mice ( A–C ), more cells in MTR ( D–F ) and ITR ( G–I ) groups had increased expression of stem cell markers nucleostemin (NS), Oct-4, and SSEA-1. Note that the extent of the immunostaining on these stem cell markers is apparently running-intensity-dependent. Positively stained cells were also counted to calculate percentage staining ( J ). (*p

    Journal: PLoS ONE

    Article Title: The Effects of Mechanical Loading on Tendons - An In Vivo and In Vitro Model Study

    doi: 10.1371/journal.pone.0071740

    Figure Lengend Snippet: The expression of stem cell markers by patellar tendon stem cells (PTSCs) in response to mouse treadmill running. TSCs were seeded in a 6-well plate, cultured and incubated with goat anti-mouse nucleostemin/Cy3-conjugated donkey anti-goat IgG antibodies ( A, D, G ), rabbit anti-mouse Oct-4/Cy3-conjugated donkey anti-rabbit IgG antibodies ( B, E, H ) or FITC-conjugated mouse anti-SSEA-1 ( C, F, I ). Compared to PTSCs from cage control mice ( A–C ), more cells in MTR ( D–F ) and ITR ( G–I ) groups had increased expression of stem cell markers nucleostemin (NS), Oct-4, and SSEA-1. Note that the extent of the immunostaining on these stem cell markers is apparently running-intensity-dependent. Positively stained cells were also counted to calculate percentage staining ( J ). (*p

    Article Snippet: Cultures were washed three times with PBS, followed by application of Cy3-conjugated donkey anti-rabbit IgG antibodies (1∶500 for Oct-4, Millipore, Billerica, MA; Cat. #AC182C), or Cy3-conjugated Donkey anti-goat IgG antibodies (1∶500 for nucleostemin, Millipore, Billerica, MA; Cat. #AC180C) for 2 hrs at room temperature.

    Techniques: Expressing, Cell Culture, Incubation, Mouse Assay, Immunostaining, Staining