cx26  (Alomone Labs)

Journal: Cerebral Cortex (New York, NY)

Article Title: Mechanisms of Spontaneous Electrical Activity in the Developing Cerebral Cortex—Mouse Subplate Zone

doi: 10.1093/cercor/bhy205

Figure Lengend Snippet: Connexin 26 and 45 immunolabeling. ( A ) P2 mouse. Composite image (Zeiss Mosaic) comprised of multiple tiles under 20× objective. Double immunolabeling of Cx26 (red) and NeuN (green) in P2 animals, showing weak expression of Cx26 in SP neurons at this age. ( B ) 100× objective. Colocalization of NeuN (green) and Cx26 (red) in the SP neurons at P2 age. ( C ) P2 mouse. Composite image comprised of multiple tiles under 20X objective. Double immunolabeling with Cx45 (red) and NeuN (green) showing expression of Cx45 in all cortical layers and the SP zone at P2 age. ( D ) 100× objective. Colocalization of NeuN (green) and Cx45 (red) in the SP neurons at P2 age. ( E ) P10 mouse. Composite image comprised of multiple tiles under a 20× objective. At P10 age, Cx26 (red) expression increases in layers 2, 3, 5 and in SP zone, compared with age P2. ( F ) Images obtained with 100× lens show colocalization of NeuN (green) and Cx26 (red) in the SP neurons at P10. ( G ) P10 mouse. Composite image comprised of multiple tiles under 20× objective. At P10, Cx45 (red) expression increases throughout the cortex and SP zone, compared with P2 mice. ( H ) 100× objective. Colocalization of NeuN (green) and Cx45 (red) in the SP neurons, at P10. Cx45 also expresses in non-neuronal cells of the white matter (arrow). Scale bars in A, C, E, and G = 100 μm; in B, D, F, and H = 20 μm. SP = subplate; WM = white matter. Str. = Striatum; Hoechst nuclei stain (blue).

Article Snippet: Rabbit polyclonal antibodies for Cx26, Cx36, and Cx45 were purchased from Alomone Labs (Jerusalem, Israel).

Techniques: Immunolabeling, Expressing, Mouse Assay, Staining

Journal: Cerebral Cortex (New York, NY)

Article Title: Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex

doi: 10.1093/cercor/bhz163

Figure Lengend Snippet: Expression profile of CX45, CX36, and CX26 transcripts in the human fetal cortex, from 17 to 23 GW. ( A ) Log10 FPKM values derived by the HISAT-StringTie pathway of data analysis. ( B ) Log10 normalized counts derived by the STAR-HTSeq-DESeq2 data analysis pathway. ( C ) Expression profile of 2 predominant transcripts of Cx45 (ENST00000592524.5 [CX45_524.5] and ENST00000426548.5 [Cx45_548.5]) in comparison with Cx36 and Cx26 with HISAT-StringTie pathway of analysis from 17 to 23 GW in the human fetal cortex. In panels A – C , n = 4, 17 GW; n = 2, 18 GW; n = 5, 19 GW; and n = 1, 23 GW. ( D ) Log10 normalized counts of the Cx and pannexin isoforms expressed in the human fetal cortex, age range of 17–23 GW (STAR-HTSeq-DESeq2 data analysis pathway) ( n = 7). ( E ) Differential expression analysis of human fetal samples at 17 and 19 GW, by the STAR-HTSeq-DESeq2 method ( n = 3, 17 GW; n = 2, 19 GW). ( F ) Sex determination based on XIST expression and ratio of X-to-Y coverage in each human subject used in the study ( n = 7). ( G ) Differential expression analysis of male ( n = 4) and female ( n = 3) human fetal samples from 17 to 19 GW, by the STAR-HTSeq-DESeq2 method. ( H ) Differential expression analysis of cortex ( n = 3) and full brain ( n = 3) human fetal samples at 17 GW by the STAR-HTSeq-DESeq2 method. * P adj

Article Snippet: Primary antibodies were purchased from Alomone Labs, Thermo Fisher Scientific, and Millipore (company, antigen [catalog number]: Alomone Labs, Cx26 [ACC-212], Cx36 [ACC-209], and Cx45 [ACC-207]; Thermo Fisher, Cx26 [512800], Cx36 [516200], Nurr1 [MA1-195], beta-3-tubulin [MA1-19582], and NeuN [MA5-33103]; Millipore, NeuN [MAB377]).

Techniques: Expressing, Derivative Assay

Journal: Cerebral Cortex (New York, NY)

Article Title: Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex

doi: 10.1093/cercor/bhz163

Figure Lengend Snippet: Cells expressing Cx26 in 3 zones of cortical mantle. ( A ) Human fetal cortex (20 GW, female). Hoechst stain. Composite (mosaic) of multiple images. ( B ) CP, ( C ) SP, and ( D ) SVZ. Green = neuronal marker, Neun; red = Cx isoform, Cx26; blue = nuclear stain by Hoechst. Scales in panels B – D , 20 μm.

Article Snippet: Primary antibodies were purchased from Alomone Labs, Thermo Fisher Scientific, and Millipore (company, antigen [catalog number]: Alomone Labs, Cx26 [ACC-212], Cx36 [ACC-209], and Cx45 [ACC-207]; Thermo Fisher, Cx26 [512800], Cx36 [516200], Nurr1 [MA1-195], beta-3-tubulin [MA1-19582], and NeuN [MA5-33103]; Millipore, NeuN [MAB377]).

Techniques: Expressing, Staining, Marker

Journal: Developmental Neurobiology

Article Title: Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss

doi: 10.1002/dneu.22279

Figure Lengend Snippet: Epithelial expression of GJB2 and GJB6 in the cochlear duct. (A‐B) The lower basal turn cochlea at W10.4 immunostained for gap junction proteins GJB2 [(A), green] and GJB6 [(B), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (A′) and (B′), respectively. The bracket outlines the prosensory domain. Scale bars = 50 µm. (C‐D) The lower basal turn cochlea at W12.2 immunostained for GJB2 [(C), green] and GJB6 [(D), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (C′) and (D′), respectively. The bracket outlines the prosensory domain. Scale bars = 50 µm. (E‐F) The lower basal turn cochlea at W14 immunostained for GJB2 ((E), green) and GJB6 ((F), green) and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (E′) and (F′), respectively. The bracket outlines the developing organ of Corti. KO = Kölliker's organ. Scale bars = 50 µm. (G–H) The lower basal turn cochlea at W14 immunostained for GJB2 [(G), green] and GJB6 [(H), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (G′) and (H′), respectively. The arrowheads points to the developing root cells. The bracket outlines the developing of Corti. KO = Kölliker's organ. Scale bars = 50 µm. (I‐J) The lower basal turn cochlea at W14 immunostained for GJB2 [(I), green] and GJB6 [(J), green] and DAPI (blue). GJB2 and GJB6 signals are shown separately in white in (I′) and (J′), respectively. The arrowheads points to the developing root cells. The bracket outlines the organ of Corti. * = autofluorescent erythrocytes. Scale bars = 50 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com .]

Article Snippet: The primary antibodies used in this study were mouse anti‐acetylated tubulin (aceTUBA, 1:500, T6793, Sigma), rabbit anti‐collagen type IV (COL4, 1:200, AB748, Chemicon), rabbit anti‐fibronectin (FN, 1:400, F3648, Sigma‐Aldrich), rabbit anti‐GJA1 (1:1000, C6219, Sigma), rabbit anti‐GJA1 (1:2000, ACC‐201, Alomone labs), rabbit anti‐GJB2 (1:100, ACC‐212, Alomone labs), rabbit anti‐GJB6 (1:100, PA511640, Thermo Scientific), rabbit anti‐GJE1 (1:1000, NBP2–14051, Novus biologicals), goat anti‐KCNJ10 (1:100, NBP1–70371), rabbit anti‐KCNQ1 (1:200, ab65092, Abcam), rabbit anti‐KCNQ1 (1:100, APC‐022, Alomone labs), rabbit anti‐KIT (1:100, A4502, Dako), rabbit anti‐laminin (LAM, 1:200, Z009701, Dako), rabbit anti‐melan‐A (1:500, NBP1–30151, Novus), mouse anti‐microphthalmia‐associated transcription factor (MITF, 1:100, M3621, Dako), mouse anti‐Na+ /K+ ‐ATPase α1 (ATP1A1, 1:200, α6F, Developmental Studies Hybridoma Bank), rabbit anti‐solute family carrier 2, member 1 (SLC2A1, 1:500, ab15309, Abcam), and goat anti‐SOX10 (1:50, sc‐17342, Santa Cruz Biotechnology).

Techniques: Expressing