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Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software <t>Actitrack</t> (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)
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Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software <t>Actitrack</t> (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)
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Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software <t>Actitrack</t> (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)
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Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software <t>Actitrack</t> (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)
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Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software <t>Actitrack</t> (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)
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Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software <t>Actitrack</t> (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)
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( a ) Comparative representation of TrkAIII <t>and</t> <t>fs-TrkA</t> exon structures. ( b ) TrkAIII qRT-PCR Assay 1 and 2 strategies, illustrating TrkAIII specificity provided by the <t>TaqMan</t> probe and by the antisense primer (R), respectively.
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Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software Actitrack (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)

Journal: Brain Structure & Function

Article Title: Temporal progression of pathological features in an α-synuclein overexpression model of Parkinson’s disease

doi: 10.1007/s00429-025-02959-9

Figure Lengend Snippet: Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software Actitrack (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)

Article Snippet: The detection of global activity, locomotion and mean velocity was fully automated using the custom-designed software Actitrack (Panlab, Spain).

Techniques: Control, Injection, Activity Assay, Software

( a ) Comparative representation of TrkAIII and fs-TrkA exon structures. ( b ) TrkAIII qRT-PCR Assay 1 and 2 strategies, illustrating TrkAIII specificity provided by the TaqMan probe and by the antisense primer (R), respectively.

Journal: Cancers

Article Title: A TaqMan-Based qRT-PCR Assay for Accurate Evaluation of the Oncogenic TrkAIII Splice Variant in Tumor cDNAs

doi: 10.3390/cancers17030471

Figure Lengend Snippet: ( a ) Comparative representation of TrkAIII and fs-TrkA exon structures. ( b ) TrkAIII qRT-PCR Assay 1 and 2 strategies, illustrating TrkAIII specificity provided by the TaqMan probe and by the antisense primer (R), respectively.

Article Snippet: Assay primers and probes were selected from NTRK1/TrkA (NM_001012331.1) and TrkAIII (GenBank: OP866787.1) sequences using Custom TaqMan ® Assay Design Tool software ( https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping/ , accessed on 7 March 2023) and were synthesized by Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Quantitative RT-PCR