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Agilent technologies custom oligonucleotide microarrays
Custom Oligonucleotide Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom oligonucleotide microarrays/product/Agilent technologies
Average 88 stars, based on 5 article reviews
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custom oligonucleotide microarrays - by Bioz Stars, 2020-05
88/100 stars

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Microarray:

Article Title: Global Transcriptome Analysis of Lactococcus garvieae Strains in Response to Temperature
Article Snippet: .. This custom oligonucleotide microarray was manufactured by Agilent Technologies on an 8x15K format. ..

Article Title: Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions
Article Snippet: .. Immunoprecipitated and input DNA samples were cohybridized to a custom DNA microarray (Agilent) and data were normalized as previously described. ..

Article Title: Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways
Article Snippet: .. Transcriptome analysis Transcriptome analysis was performed using custom DNA microarrays (8 × 15K; Agilent Technologies) previously designed based on the annotated genome sequences of S. oneidensis MR-1 according to the manufacturer’s protocol for gene expression arrays for prokaryotes (Agilent One-Color Microarray-Based Prokaryote Analysis, version 1.4, http://www.chem.agilent.com ). ..

Article Title: Transcriptome profiling of a curdlan-producing Agrobacterium reveals conserved regulatory mechanisms of exopolysaccharide biosynthesis
Article Snippet: .. The microarray is an 8 × 15 k custom DNA microarray from Agilent with probes designed using Agilent's eArray program. .. The eArray program designed probes for 5,580 of the 5,585 predicted genes in the ATCC 31749 genome with two technical replicates for each gene.

DNA Array:

Article Title: Dynamic Nucleosome Organization at hox Promoters during Zebrafish Embryogenesis
Article Snippet: .. Mononucleosome sized fragments were gel-purified and hybridized to an Agilent custom DNA array tiled with 50 bp oligonucleotides positioned every 20 bp across the seven zebrafish hox clusters. ..

Expressing:

Article Title: Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways
Article Snippet: .. Transcriptome analysis Transcriptome analysis was performed using custom DNA microarrays (8 × 15K; Agilent Technologies) previously designed based on the annotated genome sequences of S. oneidensis MR-1 according to the manufacturer’s protocol for gene expression arrays for prokaryotes (Agilent One-Color Microarray-Based Prokaryote Analysis, version 1.4, http://www.chem.agilent.com ). ..

Immunoprecipitation:

Article Title: Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions
Article Snippet: .. Immunoprecipitated and input DNA samples were cohybridized to a custom DNA microarray (Agilent) and data were normalized as previously described. ..

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  • 85
    Agilent technologies custom oligo dna microarray chips
    Clustering analysis of the expression balance between the sense and antisense genes, or the coding and noncoding genes in ES cells, fibroblast cells, brain, heart, and testis. A, B, C was based on the <t>microarray</t> data obtained with the <t>oligo</t> dT priming method, whereas the random priming method was used for the data in D, E , and F . ( A,D ) Clustering with the data of the pairs consisting of the coding genes only. When the sense gene expression is threefold more than the antisense gene expression, the color is shown in red. In the reversed situation, the color is shown in green. ( B,E ) Clustering with the data of the pairs consisting of the noncoding genes only. The coloring is the same as found in A . ( C,F ) Clustering with the data of the pairs consisting of the coding and noncoding genes. When the noncoding gene expression is threefold more than the coding gene expression, the color is shown in red. Note that this figure is intended to show the ratio between sense and antisense, or noncoding and coding expression. The absolute expression values for each gene are found in Supplemental Table 1.
    Custom Oligo Dna Microarray Chips, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom oligo dna microarray chips/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    custom oligo dna microarray chips - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    91
    Agilent technologies dna microarrays custom
    Validation of <t>DNA</t> microarray results using quantitative real-time reverse-transcription PCR (RT-qPCR). Log 2 fold-changes of transcript levels measured with DNA <t>microarrays</t> (x-axis) and RT-qPCR (y-axis) in C . botulinum ATCC 3502 continuous culture 10 min (red) and 42 h (black) after temperature up-shift from 39 to 45°C. 16S rrn transcript levels were used as a normalization reference in the RT-qPCR. Linear regression analysis showed an R 2 correlation value of 0.99 between the microarray and RT-qPCR transcription fold-change results.
    Dna Microarrays Custom, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarrays custom/product/Agilent technologies
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna microarrays custom - by Bioz Stars, 2020-05
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    85
    Agilent technologies 22k custom oligo dna microarray
    Scatter plots comparing gene expression levels in SAM and mature leaves. An Agilent rice <t>22K</t> custom <t>oligo</t> <t>DNA</t> <t>microarray</t> was used. Each spot on the array is represented by a dot in the scatter plot. The results of color-swap experiments (left, mature leaf-Cy 3/SAM-Cy 5; right, SAM-Cy 3/mature leaf-Cy 5) are shown. The red line represents the diagonal (equal expression in the two tissues). The yellow line above represents a Cy-3/Cy-5 ratio of 5:1, while that below represents a Cy-3/Cy-5 ratio of 1:5.
    22k Custom Oligo Dna Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/22k custom oligo dna microarray/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    22k custom oligo dna microarray - by Bioz Stars, 2020-05
    85/100 stars
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    85
    Agilent technologies high density custom made oligonucleotide tiling microarray analysis
    High-density custom-made tiling <t>microarray</t> analysis of the MZ twins discordant for the MSA phenotype . (a) Competitive hybridization of genomic DNA from the MSA-affected twin (HK33) versus that from his twin (HK34). (b) Dye-swap experiment of the normal twin (HK34) versus his affected twin (HK33). (c) Competitive hybridization of genomic DNA from the MSA-affected twin (HK33) versus that from a reference Japanese male (HapMap NA1900). (d) Competitive hybridization of genomic DNA from the MSA-affected twin (HK34) versus that from the reference Japanese male (HapMap NA19000). Each blue line represents a moving average ratio of log 2 (Cy5/Cy3). The blue regions indicate deletions; these loci were defined by dye-swap experiments (red region). The top map shows the positions of putative genes in the region [ 16 ].
    High Density Custom Made Oligonucleotide Tiling Microarray Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high density custom made oligonucleotide tiling microarray analysis/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Clustering analysis of the expression balance between the sense and antisense genes, or the coding and noncoding genes in ES cells, fibroblast cells, brain, heart, and testis. A, B, C was based on the microarray data obtained with the oligo dT priming method, whereas the random priming method was used for the data in D, E , and F . ( A,D ) Clustering with the data of the pairs consisting of the coding genes only. When the sense gene expression is threefold more than the antisense gene expression, the color is shown in red. In the reversed situation, the color is shown in green. ( B,E ) Clustering with the data of the pairs consisting of the noncoding genes only. The coloring is the same as found in A . ( C,F ) Clustering with the data of the pairs consisting of the coding and noncoding genes. When the noncoding gene expression is threefold more than the coding gene expression, the color is shown in red. Note that this figure is intended to show the ratio between sense and antisense, or noncoding and coding expression. The absolute expression values for each gene are found in Supplemental Table 1.

    Journal: Genome Research

    Article Title: Disclosing hidden transcripts: Mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear localized

    doi: 10.1101/gr.3155905

    Figure Lengend Snippet: Clustering analysis of the expression balance between the sense and antisense genes, or the coding and noncoding genes in ES cells, fibroblast cells, brain, heart, and testis. A, B, C was based on the microarray data obtained with the oligo dT priming method, whereas the random priming method was used for the data in D, E , and F . ( A,D ) Clustering with the data of the pairs consisting of the coding genes only. When the sense gene expression is threefold more than the antisense gene expression, the color is shown in red. In the reversed situation, the color is shown in green. ( B,E ) Clustering with the data of the pairs consisting of the noncoding genes only. The coloring is the same as found in A . ( C,F ) Clustering with the data of the pairs consisting of the coding and noncoding genes. When the noncoding gene expression is threefold more than the coding gene expression, the color is shown in red. Note that this figure is intended to show the ratio between sense and antisense, or noncoding and coding expression. The absolute expression values for each gene are found in Supplemental Table 1.

    Article Snippet: DNAFORM (Japan), and Agilent Technologies manufactured custom oligo DNA microarray chips by using this information.

    Techniques: Expressing, Microarray

    Microarray and Northern hybridization analysis of representative SAT pair. ( A ) Mapping patterns of sense (coding, 6330439J10) and antisense (noncoding, A230019L24) genes in the genome. The positions of exons are indicated as filled columns. The directions of transcription are indicated with arrows. In this figure, information associated with sense genes is shown in blue and that for antisense genes in red. ( B ) Microarray signal intensities obtained with samples labeled by oligo dT priming. ( C ) Microarray signal intensities obtained with samples labeled by random-nanomer priming. ( D,E ) Northern hybridization of sense (6330439J10) and antisense (A230019L24) genes. In each lane, 20 μg of total RNA or 1 μg of mRNA was loaded. In the Northern blot figures, the positions of 18S and 28S ribosomal RNA are indicated by arrowheads at the left edges of the blots.

    Journal: Genome Research

    Article Title: Disclosing hidden transcripts: Mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear localized

    doi: 10.1101/gr.3155905

    Figure Lengend Snippet: Microarray and Northern hybridization analysis of representative SAT pair. ( A ) Mapping patterns of sense (coding, 6330439J10) and antisense (noncoding, A230019L24) genes in the genome. The positions of exons are indicated as filled columns. The directions of transcription are indicated with arrows. In this figure, information associated with sense genes is shown in blue and that for antisense genes in red. ( B ) Microarray signal intensities obtained with samples labeled by oligo dT priming. ( C ) Microarray signal intensities obtained with samples labeled by random-nanomer priming. ( D,E ) Northern hybridization of sense (6330439J10) and antisense (A230019L24) genes. In each lane, 20 μg of total RNA or 1 μg of mRNA was loaded. In the Northern blot figures, the positions of 18S and 28S ribosomal RNA are indicated by arrowheads at the left edges of the blots.

    Article Snippet: DNAFORM (Japan), and Agilent Technologies manufactured custom oligo DNA microarray chips by using this information.

    Techniques: Microarray, Northern Blot, Hybridization, Labeling

    Overall expression of sense and antisense genes as determined by using an oligo DNA microarray. The combined expression of one SAT pair is represented by a dot. The expression in ES cells is presented in A and B , and that in fibroblast cells is presented in C and D . The pairs consisting of coding and noncoding genes are shown in A and C ( x -axis, noncoding gene; y -axis, coding gene), whereas the pairs consisting of both coding genes are shown in B and D .

    Journal: Genome Research

    Article Title: Disclosing hidden transcripts: Mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear localized

    doi: 10.1101/gr.3155905

    Figure Lengend Snippet: Overall expression of sense and antisense genes as determined by using an oligo DNA microarray. The combined expression of one SAT pair is represented by a dot. The expression in ES cells is presented in A and B , and that in fibroblast cells is presented in C and D . The pairs consisting of coding and noncoding genes are shown in A and C ( x -axis, noncoding gene; y -axis, coding gene), whereas the pairs consisting of both coding genes are shown in B and D .

    Article Snippet: DNAFORM (Japan), and Agilent Technologies manufactured custom oligo DNA microarray chips by using this information.

    Techniques: Expressing, Microarray

    Average signal intensities on microarray chips after oligo dT priming were compared with those from random priming methods. In every cell type and tissue analyzed, the average signal intensity of SAT genes was higher than that of typical genes (ESTs) when the random priming method was used for sample labeling. The change in signal intensity after the change from oligo dT priming to random priming is shown at the top of the bars.

    Journal: Genome Research

    Article Title: Disclosing hidden transcripts: Mouse natural sense-antisense transcripts tend to be poly(A) negative and nuclear localized

    doi: 10.1101/gr.3155905

    Figure Lengend Snippet: Average signal intensities on microarray chips after oligo dT priming were compared with those from random priming methods. In every cell type and tissue analyzed, the average signal intensity of SAT genes was higher than that of typical genes (ESTs) when the random priming method was used for sample labeling. The change in signal intensity after the change from oligo dT priming to random priming is shown at the top of the bars.

    Article Snippet: DNAFORM (Japan), and Agilent Technologies manufactured custom oligo DNA microarray chips by using this information.

    Techniques: Microarray, Labeling

    Validation of DNA microarray results using quantitative real-time reverse-transcription PCR (RT-qPCR). Log 2 fold-changes of transcript levels measured with DNA microarrays (x-axis) and RT-qPCR (y-axis) in C . botulinum ATCC 3502 continuous culture 10 min (red) and 42 h (black) after temperature up-shift from 39 to 45°C. 16S rrn transcript levels were used as a normalization reference in the RT-qPCR. Linear regression analysis showed an R 2 correlation value of 0.99 between the microarray and RT-qPCR transcription fold-change results.

    Journal: PLoS ONE

    Article Title: Heat shock and prolonged heat stress attenuate neurotoxin and sporulation gene expression in group I Clostridium botulinum strain ATCC 3502

    doi: 10.1371/journal.pone.0176944

    Figure Lengend Snippet: Validation of DNA microarray results using quantitative real-time reverse-transcription PCR (RT-qPCR). Log 2 fold-changes of transcript levels measured with DNA microarrays (x-axis) and RT-qPCR (y-axis) in C . botulinum ATCC 3502 continuous culture 10 min (red) and 42 h (black) after temperature up-shift from 39 to 45°C. 16S rrn transcript levels were used as a normalization reference in the RT-qPCR. Linear regression analysis showed an R 2 correlation value of 0.99 between the microarray and RT-qPCR transcription fold-change results.

    Article Snippet: Transcriptomic analysis with DNA microarrays Custom designed, in situ -synthesized DNA microarrays (8x15K; Agilent Technologies), successfully employed in C . botulinum gene expression analysis studies [ , ], were used.

    Techniques: Microarray, Polymerase Chain Reaction, Quantitative RT-PCR

    Scatter plots comparing gene expression levels in SAM and mature leaves. An Agilent rice 22K custom oligo DNA microarray was used. Each spot on the array is represented by a dot in the scatter plot. The results of color-swap experiments (left, mature leaf-Cy 3/SAM-Cy 5; right, SAM-Cy 3/mature leaf-Cy 5) are shown. The red line represents the diagonal (equal expression in the two tissues). The yellow line above represents a Cy-3/Cy-5 ratio of 5:1, while that below represents a Cy-3/Cy-5 ratio of 1:5.

    Journal: Nucleic Acids Research

    Article Title: DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells

    doi: 10.1093/nar/gkh591

    Figure Lengend Snippet: Scatter plots comparing gene expression levels in SAM and mature leaves. An Agilent rice 22K custom oligo DNA microarray was used. Each spot on the array is represented by a dot in the scatter plot. The results of color-swap experiments (left, mature leaf-Cy 3/SAM-Cy 5; right, SAM-Cy 3/mature leaf-Cy 5) are shown. The red line represents the diagonal (equal expression in the two tissues). The yellow line above represents a Cy-3/Cy-5 ratio of 5:1, while that below represents a Cy-3/Cy-5 ratio of 1:5.

    Article Snippet: To confirm the results of the expression analyses, analysis using the Agilent 22K custom oligo DNA microarray was performed to identify global gene expression patterns (Figs – ).

    Techniques: Expressing, Microarray

    Expression data for rice DNA repair genes. Scatter plots were drawn based on expression data for 90 DNA repair genes spotted on the rice 22K custom oligo DNA microarray slide. Red and green dots represent spots for NER genes and the CPD photolyase gene, respectively. Blue dots represent spots for other dark repair genes (BER, MMR, HR, etc.). The red line represents the diagonal line.

    Journal: Nucleic Acids Research

    Article Title: DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells

    doi: 10.1093/nar/gkh591

    Figure Lengend Snippet: Expression data for rice DNA repair genes. Scatter plots were drawn based on expression data for 90 DNA repair genes spotted on the rice 22K custom oligo DNA microarray slide. Red and green dots represent spots for NER genes and the CPD photolyase gene, respectively. Blue dots represent spots for other dark repair genes (BER, MMR, HR, etc.). The red line represents the diagonal line.

    Article Snippet: To confirm the results of the expression analyses, analysis using the Agilent 22K custom oligo DNA microarray was performed to identify global gene expression patterns (Figs – ).

    Techniques: Expressing, Microarray

    High-density custom-made tiling microarray analysis of the MZ twins discordant for the MSA phenotype . (a) Competitive hybridization of genomic DNA from the MSA-affected twin (HK33) versus that from his twin (HK34). (b) Dye-swap experiment of the normal twin (HK34) versus his affected twin (HK33). (c) Competitive hybridization of genomic DNA from the MSA-affected twin (HK33) versus that from a reference Japanese male (HapMap NA1900). (d) Competitive hybridization of genomic DNA from the MSA-affected twin (HK34) versus that from the reference Japanese male (HapMap NA19000). Each blue line represents a moving average ratio of log 2 (Cy5/Cy3). The blue regions indicate deletions; these loci were defined by dye-swap experiments (red region). The top map shows the positions of putative genes in the region [ 16 ].

    Journal: Molecular Brain

    Article Title: Copy number loss of (src homology 2 domain containing)-transforming protein 2 (SHC2) gene: discordant loss in monozygotic twins and frequent loss in patients with multiple system atrophy

    doi: 10.1186/1756-6606-4-24

    Figure Lengend Snippet: High-density custom-made tiling microarray analysis of the MZ twins discordant for the MSA phenotype . (a) Competitive hybridization of genomic DNA from the MSA-affected twin (HK33) versus that from his twin (HK34). (b) Dye-swap experiment of the normal twin (HK34) versus his affected twin (HK33). (c) Competitive hybridization of genomic DNA from the MSA-affected twin (HK33) versus that from a reference Japanese male (HapMap NA1900). (d) Competitive hybridization of genomic DNA from the MSA-affected twin (HK34) versus that from the reference Japanese male (HapMap NA19000). Each blue line represents a moving average ratio of log 2 (Cy5/Cy3). The blue regions indicate deletions; these loci were defined by dye-swap experiments (red region). The top map shows the positions of putative genes in the region [ 16 ].

    Article Snippet: High-density custom-made oligonucleotide tiling microarray analysis We fabricated a custom-made microarray comprising 60-mer probes (Agilent Technologies) targeting a 350-kb genomic region in the distal subtelomeric region of 19p13.3 (Chr.

    Techniques: Microarray, Hybridization

    Structure of copy number loss in the 350-kb subtelomeric region on 19p13.3 resolved by high-density tiling microarray . The moving average log 2 ratio ( y -axis) is plotted against the genomic position along the chromosome ( x -axis). HK06, HK12, HK15, HK19, HK23, and HK28 represent 6 patients with MSA and HK60c and HK78c represent controls. The dark lines indicate the copy number loss. The light lines and dotted lines indicate the normal range and median of the average log 2 ratios for probes among normal individuals ( n = 25), respectively.

    Journal: Molecular Brain

    Article Title: Copy number loss of (src homology 2 domain containing)-transforming protein 2 (SHC2) gene: discordant loss in monozygotic twins and frequent loss in patients with multiple system atrophy

    doi: 10.1186/1756-6606-4-24

    Figure Lengend Snippet: Structure of copy number loss in the 350-kb subtelomeric region on 19p13.3 resolved by high-density tiling microarray . The moving average log 2 ratio ( y -axis) is plotted against the genomic position along the chromosome ( x -axis). HK06, HK12, HK15, HK19, HK23, and HK28 represent 6 patients with MSA and HK60c and HK78c represent controls. The dark lines indicate the copy number loss. The light lines and dotted lines indicate the normal range and median of the average log 2 ratios for probes among normal individuals ( n = 25), respectively.

    Article Snippet: High-density custom-made oligonucleotide tiling microarray analysis We fabricated a custom-made microarray comprising 60-mer probes (Agilent Technologies) targeting a 350-kb genomic region in the distal subtelomeric region of 19p13.3 (Chr.

    Techniques: Microarray