ctrl shrna (Millipore)
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MISSION shRNA Product Offerings
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Sigma Aldrich is a global supplier of leading RNA interference RNAi tools The MISSION TRC shRNA libraries are a comprehensive collection of 150 000 pre cloned shRNA constructs targeting 15 000 human and 15 000 mouse genes These lentiviral based constructs allow flexible delivery of long or short term silencing in a broad range of mammalian cell types including primary and non dividing cells The MISSION TRC shRNA libraries provide solutions for pathway elucidation target identification and are ideal for high content screens The libraries are offered with a variety of options and formats Entire libraries gene family sets target sets or individual clones are available as bacterial glycerol stocks purified DNA and lentiviral transduction particles To find more information including protocols references and frequently asked questions visit the RNAi website
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shrna
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Millipore
ctrl shrna
Sigma Aldrich is a global supplier of leading RNA interference RNAi tools The MISSION TRC shRNA libraries are a comprehensive collection of 150 000 pre cloned shRNA constructs targeting 15 000 human and 15 000 mouse genes These lentiviral based constructs allow flexible delivery of long or short term silencing in a broad range of mammalian cell types including primary and non dividing cells The MISSION TRC shRNA libraries provide solutions for pathway elucidation target identification and are ideal for high content screens The libraries are offered with a variety of options and formats Entire libraries gene family sets target sets or individual clones are available as bacterial glycerol stocks purified DNA and lentiviral transduction particles To find more information including protocols references and frequently asked questions visit the RNAi website
https://www.bioz.com/result/ctrl shrna/product/Millipore
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Sigma Aldrich is a global supplier of leading RNA interference RNAi tools The MISSION TRC shRNA libraries are a comprehensive collection of 150 000 pre cloned shRNA constructs targeting 15 000 human and 15 000 mouse genes These lentiviral based constructs allow flexible delivery of long or short term silencing in a broad range of mammalian cell types including primary and non dividing cells The MISSION TRC shRNA libraries provide solutions for pathway elucidation target identification and are ideal for high content screens The libraries are offered with a variety of options and formats Entire libraries gene family sets target sets or individual clones are available as bacterial glycerol stocks purified DNA and lentiviral transduction particles To find more information including protocols references and frequently asked questions visit the RNAi website
https://www.bioz.com/result/ctrl shrna/product/Millipore
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ctrl shrna - by Bioz Stars,
2021-03
97/100 stars
Images
1) Product Images from "Testicular Lmcd1 regulates phagocytosis by Sertoli cells through modulation of NFAT1/Txlna signaling pathway"
Article Title: Testicular Lmcd1 regulates phagocytosis by Sertoli cells through modulation of NFAT1/Txlna signaling pathway
Journal: Aging Cell
doi: 10.1111/acel.13217
Figure Legend Snippet: Lmcd1 depletion compromises TXLNA‐mediated testicular phagocytosis. (a) Expression levels of different key factors essential for testicular phagocytosis in Lmcd1 siRNA‐treated testis were determined using RT‐qPCR. ** p
Techniques Used: Expressing, Quantitative RT-PCR
Figure Legend Snippet: Effects of ablation of Lmcd1 expression in vivo on testicular morphology and GC apoptosis. (a) Schematic representation of the experimental procedures used in the in vivo siRNA treatment. (b) Effects of endogenous Lmcd1 knockdown in testis by in vivo siRNA treatment were confirmed by RT‐qPCR at transcriptional level. (c) Effects of endogenous Lmcd1 knockdown in testis by in vivo siRNA treatment were confirmed by immunohistochemistry at translational level. Scale bar, 25 μm. (d) Effect of in vivo siRNA treatment on mouse testicular morphology was evaluated in H E‐stained transverse testis sections. Scale bar, 25 μm. Asterisks denote vacuoles. (e) Effect of in vivo siRNA treatment on GC apoptosis was assessed using TUNEL staining, followed by the quantification of TUNEL‐positive cells (right panel)
Techniques Used: Expressing, In Vivo, Quantitative RT-PCR, Immunohistochemistry, Staining, TUNEL Assay
Figure Legend Snippet: Functional relevance and paracrine control of LMCD1 expression during testicular phagocytosis. (a) Effect of siRNA treatment on testicular phagocytosis in vivo was evaluated using Oil Red O staining. (b) Upper panel: Representative pictures of residual body (RB) binding and phagocytized by Ctrl siRNA or Lmcd1 siRNA‐treated 15P‐1 cells 6 h after addition of RBs. Arrowheads denote the ingested and plasma membrane‐bound RBs, whereas arrows denote the cells with no phagocytized RBs. Lower panel: Kinetics of RB binding and phagocytosis by SCs (the binding and phagocytosis index) were calculated as the ratio of positive green signals/nuclei number in each well. Quantitative values were presented as mean ± SD of three independent experiments (* p
Techniques Used: Functional Assay, Expressing, In Vivo, Staining, Binding Assay
2) Product Images from "Noradrenaline from Locus Coeruleus Neurons Acts on Pedunculo-Pontine Neurons to Prevent REM Sleep and Induces Its Loss-Associated Effects in Rats"
Article Title: Noradrenaline from Locus Coeruleus Neurons Acts on Pedunculo-Pontine Neurons to Prevent REM Sleep and Induces Its Loss-Associated Effects in Rats
Journal: eNeuro
doi: 10.1523/ENEURO.0108-16.2016
Figure Legend Snippet: Under stereotaxic surgery, either TH-shRNA or Ctrl-shRNA was microinjected into the right and the left LC, respectively, in the same rat in three groups (I, II, and III). a , b , Eight days after injection, the left and right LCs were punched from group I ( n = 5) and group II ( n = 4) rats to estimate TH mRNA ( a ) and TH protein ( b ), respectively. c , Group III ( n = 5) rat brains were taken out, and sections containing LC were immunostained with anti-TH antibodies. a , For the normalization of TH gene expression, both GAPDH and TBP were taken as reference genes. Taking the TH mRNA expression in the Ctrl-shRNA injected rats as 100%, the percentage change TH mRNA expression in TH-shRNA injected rats was estimated. The histogram shows that the TH mRNA was significantly downregulated by ∼74% (*** p
Techniques Used: shRNA, Injection, Expressing
Figure Legend Snippet: Percentage (±SEM) of total recording time spent in waking state, NREMS, and REMS 8 d after TH-shRNA or Ctrl-shRNA microinjection into the LC and Sal or NA microinjection into the PPT (on the day of recording), and 21 d after TH-shRNA microinjection. a–c , The histograms show analyses of total recording periods of 12 h (10:00 A.M. to 10:00 P.M.; a ), 8 h (10:00 A.M. to 6:00 P.M.; b ), and 4 h (6:00 to 10:00 P.M.; c ). Abbreviations are as in the text. *Compared with baseline values and Ctrl-shRNA injection; $compared with TH-shRNA injection into LC and NA injection into PPT or 3 weeks after the injection of TH-shRNA into LC. Significance level: *** p
Techniques Used: shRNA, Injection
Figure Legend Snippet: Changes in REMS frequency per hour (top) and REMS duration/episode (s; bottom) during the recording of TH-shRNA or Ctrl-shRNA 8 d after microinjection into LC and Sal or NA microinjection into PPT (on the recording day), and 21 d after TH-shRNA microinjection. a–d , The histograms show analyses for the total recording period of 12 h (10:00 A.M. to 10:00 P.M.; a , c ), and 8 h (10:00 A.M. to 6:00 P.M.) and 4 h (6:00 to 10:00 P.M.; b , d ). Abbreviations are as in the text. *Compared with baseline and Ctrl-shRNA; $compared with TH-shRNA injection into LC and NA injection into PPT or 3 weeks after the injection of TH-shRNA into the LC. Significance level: *** p
Techniques Used: shRNA, Injection
Figure Legend Snippet: a , Representative SleepSign-recorded simultaneous traces of EEG, EOG, and EMG showing waking state, NREMS, and REMS episodes in one rat treated with TH-shRNA injection into LC and injected with saline or NA into the PPT (as labeled on the figure). b , The power spectrum of average FFT (Fast Fourier Transformation) of EEG of all the REMS episodes during the 10:00 A.M. to 10:00 P.M. recording period of one rat under different conditions. Dashed line shows the data of the rat with TH-shRNA injection into LC and saline injection into PPT (total number of episodes of REMS = 131 ± 10), while the continuous line shows TH-shRNA injection into LC and NA injection into PPT (total number of episodes of REMS = 91 ± 13). It shows that the theta waves (5–7.5 Hz) peaked in the background of other frequencies. The histogram in the inset shows the average power of the theta waves (5–7.5 Hz) only of the rats shown in the line plot under the two conditions. It shows that the average power of theta waves was comparable under the two treatment conditions.
Techniques Used: shRNA, Injection, Labeling, Transformation Assay
Figure Legend Snippet: a , Diagrammatic representation of rat brain atlas sections through PPT (left) and LC (right) showing implantation of guide cannulae for microinjections into respective sites. b , c , Photomicrographs of representative histological hemisections of experimental rat brains through ( b ) PPT (neutral red stained) and ( c ) LC (TH antibody immunostained) aligned against corresponding rat brain atlas hemisection have been shown. Reconstructed microinjection on-target sites of NA into PPT ( n = 5) have been shown (open circle) on the right half of the brain atlas hemisection ( b ). As the TH-shRNA was microinjected into the LC during surgery and the injector cannula was removed, it was extremely difficult to trace the extension of the cannula tract on the histological sections. Hence, we confirmed the microinjection site by identifying very few TH-positive neurons in the anatomical LC site (as marked). 4V, 4th ventricle; Aq-, aqueduct; DMTg, dorsomedial tegmental area; MnR, medial raphe nucleus; Pn, pontine nuclei; RIP, raphe interpositus nucleus; scp, superior cerebellar penducle.
Techniques Used: Staining, shRNA
Figure Legend Snippet: Changes in REMS frequency per hour (top) and REMS duration per episode (s; bottom) 24 h after TH-siRNA or Ctrl-siRNA microinjection into LC. a–d , Analyses for the total period of 12 h (10:00 A.M. to 10:00 P.M.; a , c ), 8 h (10:00 A.M. to 6:00 P.M.; b , d ), and 4 h (6:00 to 10:00 P.M.; b , d ) are shown. *Compared with baseline and Ctrl-siRNA values. Significance level: *** p
Techniques Used:
Figure Legend Snippet: a , Diagrammatic representation of protocol of experiment 3. b , Percentage (mean ± SEM) changes in Na-K ATPase activity in the brain homogenate of rats deprived of REMS after microinjection of TH-siRNA or Ctrl-siRNA bilaterally into the LC compared with the Na-K ATPase activity of FMC rats taken as 100%. c , Percentage (mean ± SEM) changes in Na-K ATPase activity in the brain homogenate of rats deprived of REMS after microinjection of TH-shRNA or Ctrl-shRNA bilaterally into the LC compared with Na-K ATPase activity in FMC rats taken as 100%. d , Percentage (mean ± SEM) change in TH protein expression in the brains of rats deprived of REMS for 96 h after the microinjection of TH-shRNA and Ctrl-shRNA bilaterally into the LC relative to FMC control taken as 100% are shown. *Compared with FMC; $compared with Ctrl-shRNA/Ctrl-siRNA in LC and subjected to REMSD. Significance level: *** p
Techniques Used: Activity Assay, shRNA, Expressing
Figure Legend Snippet: a , A representative photomicrograph of a neutral red-stained histological hemisection through LC showing the TH-siRNA microinjection site aligned with the corresponding hemisection of the rat brain atlas is shown. On-target microinjection sites (open circles) from five rat brains have been reconstructed on the left hemisection, which also shows one off-target site (black solid circle). 4V, 4th ventricle; DMTg, dorsomedial tegmental area; RIP, raphe interpositus nucleus. b–d , Percentage (±SEM) of time spent in waking state, NREMS, and REMS 24 h after TH-siRNA or Ctrl-siRNA microinjection into LC during the total recording period of 12 h (10:00 A.M. to 10:00 P.M.; b ), 8 h (10:00 A.M. to 6:00 P.M.; c ), and 4 h (6:00 to 10:00 P.M.; d ) are shown. *Compared with baseline and Ctrl-siRNA values. Significance level: *** p
Techniques Used: Staining
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