Journal: Scientific Reports

Article Title: DeepSentRec: a deep learning-based sentiment-aware product recommendation system

doi: 10.1038/s41598-026-45953-9

Figure Lengend Snippet: Comparison of RLRanker-PPO with traditional ranking methods on Amazon and Kaggle datasets—(a) CTR, (b) Hit Rate@10, (c) NDCG@10, and (d) Engagement Score.

Article Snippet: Figure (a) depicts the Click-Through Rate (CTR), where RLRanker-PPO exceeds the baselines by a large margin of 24.96% on Amazon and 23.41% on Kaggle.

Techniques: Comparison

Journal: Nature Communications

Article Title: A neurotoxic cryptic peptide arising from TDP-43-dependent cryptic splicing of PKN1

doi: 10.1038/s41467-026-68916-0

Figure Lengend Snippet: a Boxplot of human parahippocampal transcriptome data from MSBB showing activated PKN1-5a1 cryptic splicing in AD samples across Braak stages. Box plots show median (center line), the upper and lower quantiles (box), and the range of the data (whiskers). b Schematic of bilateral hippocampal AAV injection in C57BL/6J mice and subsequent behavioral testing. c Morris water maze showing prolonged escape latency in AAV-PKFL and AAV-PKN207 mice (AAV-CTR n = 9, AAV-PKFL n = 10, AAV-PKN207 n = 10, repeated-measures two-way ANOVA). d CSF NEFL levels are elevated in AAV-PKN207 mice (AAV-CTR n = 10, AAV-PKFL n = 20, AAV-PKN207 n = 20; one-way ANOVA). e Primary hippocampal neurons expressing PKFL or PKN207 showing increased LDH release ( n = 4, one-way ANOVA). f KEGG enrichment of DEPs highlights pathways linked to synaptic function and neurodegeneration. g Heatmap showing downregulation of LTP-related genes in PKN207-expressing mice. h STRING network of NEFL-interacting proteins (red: upregulated; blue: downregulated). i Western blots of neurofilament proteins in N2a cells overexpressing PKFL or PKN207. j Hippocampal LTP is impaired in AAV-PKN207 mice; fEPSP slopes are shown (5 mice, 10 slices; one-way ANOVA). Source data are provided as a Source Data file.

Article Snippet: Hippocampus stored at −80 °C from AAV-CTR, AAV-PKN207, and AAV-PKFL mice, with three biological replicates per group, following protein extraction and trypsin digestion, peptides were combined in equal amounts and submitted to Shanghai Bioprofile Biotechnology Co., Ltd. for data-independent acquisition (DIA) using the Orbitrap Astral platform.

Techniques: Injection, Expressing, Western Blot

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, CTR-1, FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Expressing, Western Blot, Comparison, Fluorescence, Immunocytochemistry

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: Disruption of copper homeostasis as a primary mechanism behind TSPO-induced mitochondrial structural and functional abnormalities. A , B , C Comparison of FDX-1 mRNA and protein expression in FDX-1 knockdown cells and the control siRNA cells ( n = 3). D Comparison of copper ion content in the LPS-stimulated BV-2 cells in each group ( n = 5). E Representative images of ATP7A and β-actin expression in LPS-stimulated BV-2 cells by western blot. F Comparison of ATP7A expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Comparison of CTR-1 expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). H Comparison of ATP7A expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). I Quantification of the fluorescence intensity of the ROS using Image-Pro Plus ( n = 3). J Representative images showing the ROS expression in the LPS-stimulated BV-2 cells. Scale bar, 20 µm. K TEM examined the ultrastructure of cells, × 10,000. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Disruption, Functional Assay, Comparison, Expressing, Knockdown, Control, Western Blot, Quantitative RT-PCR, Fluorescence