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    Name:
    Actin Antibody
    Description:
    Anti Actin Antibody C 2 is a mouse monoclonal IgG1 kappa light chain Actin antibody provided at 200 µg ml specific for an epitope mapping between amino acids 357 375 at the C terminus of Actin of human origin Actin Antibody C 2 is recommended for detection of a broad range of Actin isoforms of mouse rat human and Xenopus origin by WB IP IF IHC P FCM and ELISA also reactive with additional species including and equine canine bovine porcine and avian Anti Actin Antibody C 2 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to TRITC PerCP PerCP Cy5 5 or Alexa Fluor 405 for IF IHC P and FCM blocking peptide sc 8432 P Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of Actin C 2 sc 8432
    Catalog Number:
    SC-8432
    Price:
    None
    Category:
    Antibodies Primary Antibodies and ImmunoCruz Conjugates Structural Proteins Actin Antibodies Actin Antibody C 2
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    Structured Review

    Santa Cruz Biotechnology ctp
    Anti Actin Antibody C 2 is a mouse monoclonal IgG1 kappa light chain Actin antibody provided at 200 µg ml specific for an epitope mapping between amino acids 357 375 at the C terminus of Actin of human origin Actin Antibody C 2 is recommended for detection of a broad range of Actin isoforms of mouse rat human and Xenopus origin by WB IP IF IHC P FCM and ELISA also reactive with additional species including and equine canine bovine porcine and avian Anti Actin Antibody C 2 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to TRITC PerCP PerCP Cy5 5 or Alexa Fluor 405 for IF IHC P and FCM blocking peptide sc 8432 P Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of Actin C 2 sc 8432
    https://www.bioz.com/result/ctp/product/Santa Cruz Biotechnology
    Average 99 stars, based on 1 article reviews
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    Related Articles

    Western Blot:

    Article Title: Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer
    Article Snippet: .. The following antibodies and reagents were used: Anti-SREBF1 (Proteintech, 14088-1-AP, 1:1000 for western blotting and 4 μg for ChIP), anti-KLF5 (Santa Cruz Biotechnology, sc-398409X, 1:1000 for western blotting, and 4 μg for ChIP), anti-TP63 (R & D Systems, AF1916-SP, 1:1000), anti-Actin (Santa Cruz Biotechnology, sc-8432, 1:2000), anti-ACLY (Cell Signaling Technology, 4332, 1:1000), Anti-FASN (Cell Signaling Technology, 3180, 1:1000), anti-GAPDH (Cell Signaling Technology, 2118, 1:2000), anti-mTOR (Cell Signaling Technology, 2972S, 1:1000), anti-Phospho-mTOR (S2448) (Cell Signaling Technology, 5536T, 1:1000), anti-Phospho-MEK1 (Ser298) (Cell Signaling Technology, 9128S, 1:1000), anti-MEK1/2(D1A5) Rabbit (Cell Signaling Technology, 9124S, 1:1000), anti-p70 S6 Kinase (Cell Signaling Technology, 9202S, 1:1000), anti-Phospho-p70 S6 Kinase (Cell Signaling Technology, 9205S, 1:1000), anti-mouse IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 115-035-003, 1:10000), anti-rabbit IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 111-035-144, 1:10000), anti-goat IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 705-035-003, 1:10000), HCS LipidTOX™ Green Neutral Lipid Stain (Thermo Scientific, H34475, 1:100), Lipofectamine RNAiMAX (Thermo Scientific, 13778150), and Fatostatin (Cayman Chemical, 12562). ..

    Article Title: Oncogenic RABL6A promotes NF1-associated MPNST progression in vivo
    Article Snippet: .. Antibodies used for western blotting include those specific for: c-Myc (1:1000, ab32072) from Abcam, p27 (1:1000, #3686) from Cell Signaling Technology, and p53 (1:200, FL-393, sc-6243), p16 (1:200, F-12, sc-1661), and β-actin (1:500, sc-8432) from Santa Cruz Biotechnology. ..

    Chromatin Immunoprecipitation:

    Article Title: Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer
    Article Snippet: .. The following antibodies and reagents were used: Anti-SREBF1 (Proteintech, 14088-1-AP, 1:1000 for western blotting and 4 μg for ChIP), anti-KLF5 (Santa Cruz Biotechnology, sc-398409X, 1:1000 for western blotting, and 4 μg for ChIP), anti-TP63 (R & D Systems, AF1916-SP, 1:1000), anti-Actin (Santa Cruz Biotechnology, sc-8432, 1:2000), anti-ACLY (Cell Signaling Technology, 4332, 1:1000), Anti-FASN (Cell Signaling Technology, 3180, 1:1000), anti-GAPDH (Cell Signaling Technology, 2118, 1:2000), anti-mTOR (Cell Signaling Technology, 2972S, 1:1000), anti-Phospho-mTOR (S2448) (Cell Signaling Technology, 5536T, 1:1000), anti-Phospho-MEK1 (Ser298) (Cell Signaling Technology, 9128S, 1:1000), anti-MEK1/2(D1A5) Rabbit (Cell Signaling Technology, 9124S, 1:1000), anti-p70 S6 Kinase (Cell Signaling Technology, 9202S, 1:1000), anti-Phospho-p70 S6 Kinase (Cell Signaling Technology, 9205S, 1:1000), anti-mouse IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 115-035-003, 1:10000), anti-rabbit IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 111-035-144, 1:10000), anti-goat IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 705-035-003, 1:10000), HCS LipidTOX™ Green Neutral Lipid Stain (Thermo Scientific, H34475, 1:100), Lipofectamine RNAiMAX (Thermo Scientific, 13778150), and Fatostatin (Cayman Chemical, 12562). ..

    Staining:

    Article Title: Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer
    Article Snippet: .. The following antibodies and reagents were used: Anti-SREBF1 (Proteintech, 14088-1-AP, 1:1000 for western blotting and 4 μg for ChIP), anti-KLF5 (Santa Cruz Biotechnology, sc-398409X, 1:1000 for western blotting, and 4 μg for ChIP), anti-TP63 (R & D Systems, AF1916-SP, 1:1000), anti-Actin (Santa Cruz Biotechnology, sc-8432, 1:2000), anti-ACLY (Cell Signaling Technology, 4332, 1:1000), Anti-FASN (Cell Signaling Technology, 3180, 1:1000), anti-GAPDH (Cell Signaling Technology, 2118, 1:2000), anti-mTOR (Cell Signaling Technology, 2972S, 1:1000), anti-Phospho-mTOR (S2448) (Cell Signaling Technology, 5536T, 1:1000), anti-Phospho-MEK1 (Ser298) (Cell Signaling Technology, 9128S, 1:1000), anti-MEK1/2(D1A5) Rabbit (Cell Signaling Technology, 9124S, 1:1000), anti-p70 S6 Kinase (Cell Signaling Technology, 9202S, 1:1000), anti-Phospho-p70 S6 Kinase (Cell Signaling Technology, 9205S, 1:1000), anti-mouse IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 115-035-003, 1:10000), anti-rabbit IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 111-035-144, 1:10000), anti-goat IgG-HRP (Jackson ImmunoResearch Laboratories, Inc., 705-035-003, 1:10000), HCS LipidTOX™ Green Neutral Lipid Stain (Thermo Scientific, H34475, 1:100), Lipofectamine RNAiMAX (Thermo Scientific, 13778150), and Fatostatin (Cayman Chemical, 12562). ..

    Incubation:

    Article Title: KYP-2047, an Inhibitor of Prolyl-Oligopeptidase, Reduces GlioBlastoma Proliferation through Angiogenesis and Apoptosis Modulation
    Article Snippet: .. To ascertain that those blots were loaded with equal amounts of protein lysate, they were also incubated with β-actin antibody (for cytosolic fraction 1:500; Santa Cruz Biotechnology, Dallas, TX, USA; sc-8432) or lamin A/C (for nuclear fraction 1:500, Santa Cruz Biotechnology, Dallas, TX, USA; sc-376248). ..

    Article Title: KYP-2047, an Inhibitor of Prolyl-Oligopeptidase, Reduces GlioBlastoma Proliferation through Angiogenesis and Apoptosis Modulation
    Article Snippet: .. To ascertain that blots were loaded with equal amounts of protein lysate, they were also incubated with the antibody β-actin for cytosolic fraction (1:500; Santa Cruz Biotechnology; Dallas, TX, USA. sc-8432) and lamin A/C for nuclear fraction (1:500; Santa Cruz Biotechnology; Dallas, TX, USA, sc-376248). ..

    other:

    Article Title: Multi-Layer Nanofibrous PCL Scaffold-Based Colon Cancer Cell Cultures to Mimic Hypoxic Tumor Microenvironment for Bioassay
    Article Snippet: The following antibodies were used for the study: Alexa Fluor 594 phalloidin (Thermo Fisher Scientific, Waltham, MA, USA), anti-HIF-1α (R & D Systems, Minneapolis, MN, USA), anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA), anti-F-actin (Santa Cruz Biotechnology, Dallas, TX, USA), anti-BrdU (Thermo Fisher Scientific, Waltham, MA, USA), anti-pimonidazole (Hypoxyprobe Inc.), anti-Ki67 (Cell Signaling Technology, Beverly, MA, USA), and cleaved caspase 3 (Cell Signaling Technology, Beverly, MA, USA).

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  • 99
    Santa Cruz Biotechnology rabbit anti ctp synthase y88
    Mutations in cytoophidium-forming isoform of <t>CTP</t> <t>synthase</t> result in growth defects in larval mitotic tissues. A: Drosophila larval internal organs are shown. The larval brain (Br) is located in the anterior of the larvae, with the eye (ed) and leg discs (ld) attached to it. The wing discs (wd) are also located close to the larval brain (larvae schematics modified from Jeibmann and Paulus, 2009 ). B and C: Wild-type and CTPsyn d06966 mutants were stained for Prospero, a marker for differentiated stem cells daughters, and Hoechst 33342 for nuclei. CTPsyn d06966 mutants have smaller larval brains with smaller optic lobes than wild-type larval brains. Most of the Prospero-positive cells in the central brain (CB) and ventral nerve cord (VNC) were, however, present in the CTPsyn d06966 mutants. Eye (ed) and leg discs (ld) in CTPsyn mutant larvae were also smaller than those in control y w larvae. D and E: wing discs (wg). F and G: the testes in CTPsyn d06966 mutant (G) were smaller than those in y w animals (F) . HTS, Hu-li tai shao, was used as a marker for fusome in testes, and the anterior ends of larval testes are marked with asterisks. Scale bars, 100 μm.
    Rabbit Anti Ctp Synthase Y88, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ctp synthase y88/product/Santa Cruz Biotechnology
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti ctp synthase y88 - by Bioz Stars, 2021-09
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    99
    Santa Cruz Biotechnology ctp
    Mutations in cytoophidium-forming isoform of <t>CTP</t> <t>synthase</t> result in growth defects in larval mitotic tissues. A: Drosophila larval internal organs are shown. The larval brain (Br) is located in the anterior of the larvae, with the eye (ed) and leg discs (ld) attached to it. The wing discs (wd) are also located close to the larval brain (larvae schematics modified from Jeibmann and Paulus, 2009 ). B and C: Wild-type and CTPsyn d06966 mutants were stained for Prospero, a marker for differentiated stem cells daughters, and Hoechst 33342 for nuclei. CTPsyn d06966 mutants have smaller larval brains with smaller optic lobes than wild-type larval brains. Most of the Prospero-positive cells in the central brain (CB) and ventral nerve cord (VNC) were, however, present in the CTPsyn d06966 mutants. Eye (ed) and leg discs (ld) in CTPsyn mutant larvae were also smaller than those in control y w larvae. D and E: wing discs (wg). F and G: the testes in CTPsyn d06966 mutant (G) were smaller than those in y w animals (F) . HTS, Hu-li tai shao, was used as a marker for fusome in testes, and the anterior ends of larval testes are marked with asterisks. Scale bars, 100 μm.
    Ctp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctp/product/Santa Cruz Biotechnology
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ctp - by Bioz Stars, 2021-09
    99/100 stars
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    92
    Santa Cruz Biotechnology anti ctp synthase ctpsyn
    Mutations in cytoophidium-forming isoform of <t>CTP</t> <t>synthase</t> result in growth defects in larval mitotic tissues. A: Drosophila larval internal organs are shown. The larval brain (Br) is located in the anterior of the larvae, with the eye (ed) and leg discs (ld) attached to it. The wing discs (wd) are also located close to the larval brain (larvae schematics modified from Jeibmann and Paulus, 2009 ). B and C: Wild-type and CTPsyn d06966 mutants were stained for Prospero, a marker for differentiated stem cells daughters, and Hoechst 33342 for nuclei. CTPsyn d06966 mutants have smaller larval brains with smaller optic lobes than wild-type larval brains. Most of the Prospero-positive cells in the central brain (CB) and ventral nerve cord (VNC) were, however, present in the CTPsyn d06966 mutants. Eye (ed) and leg discs (ld) in CTPsyn mutant larvae were also smaller than those in control y w larvae. D and E: wing discs (wg). F and G: the testes in CTPsyn d06966 mutant (G) were smaller than those in y w animals (F) . HTS, Hu-li tai shao, was used as a marker for fusome in testes, and the anterior ends of larval testes are marked with asterisks. Scale bars, 100 μm.
    Anti Ctp Synthase Ctpsyn, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ctp synthase ctpsyn/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Mutations in cytoophidium-forming isoform of CTP synthase result in growth defects in larval mitotic tissues. A: Drosophila larval internal organs are shown. The larval brain (Br) is located in the anterior of the larvae, with the eye (ed) and leg discs (ld) attached to it. The wing discs (wd) are also located close to the larval brain (larvae schematics modified from Jeibmann and Paulus, 2009 ). B and C: Wild-type and CTPsyn d06966 mutants were stained for Prospero, a marker for differentiated stem cells daughters, and Hoechst 33342 for nuclei. CTPsyn d06966 mutants have smaller larval brains with smaller optic lobes than wild-type larval brains. Most of the Prospero-positive cells in the central brain (CB) and ventral nerve cord (VNC) were, however, present in the CTPsyn d06966 mutants. Eye (ed) and leg discs (ld) in CTPsyn mutant larvae were also smaller than those in control y w larvae. D and E: wing discs (wg). F and G: the testes in CTPsyn d06966 mutant (G) were smaller than those in y w animals (F) . HTS, Hu-li tai shao, was used as a marker for fusome in testes, and the anterior ends of larval testes are marked with asterisks. Scale bars, 100 μm.

    Journal: Journal of Genetics and Genomics

    Article Title: CTP Synthase Is Required for Optic Lobe Homeostasis in Drosophila

    doi: 10.1016/j.jgg.2015.04.006

    Figure Lengend Snippet: Mutations in cytoophidium-forming isoform of CTP synthase result in growth defects in larval mitotic tissues. A: Drosophila larval internal organs are shown. The larval brain (Br) is located in the anterior of the larvae, with the eye (ed) and leg discs (ld) attached to it. The wing discs (wd) are also located close to the larval brain (larvae schematics modified from Jeibmann and Paulus, 2009 ). B and C: Wild-type and CTPsyn d06966 mutants were stained for Prospero, a marker for differentiated stem cells daughters, and Hoechst 33342 for nuclei. CTPsyn d06966 mutants have smaller larval brains with smaller optic lobes than wild-type larval brains. Most of the Prospero-positive cells in the central brain (CB) and ventral nerve cord (VNC) were, however, present in the CTPsyn d06966 mutants. Eye (ed) and leg discs (ld) in CTPsyn mutant larvae were also smaller than those in control y w larvae. D and E: wing discs (wg). F and G: the testes in CTPsyn d06966 mutant (G) were smaller than those in y w animals (F) . HTS, Hu-li tai shao, was used as a marker for fusome in testes, and the anterior ends of larval testes are marked with asterisks. Scale bars, 100 μm.

    Article Snippet: Primary antibodies used in this study included: mouse monoclonal anti-Notch antibody (intracellular domain) C17.9C6 (1:100), mouse monoclonal anti-Repo 8D12 (1:100), mouse monoclonal Abdac1-1 antibody for Dachshund (1:100) from Developmental Studies Hybridoma Bank (DSHB, USA), rabbit anti-CTP synthase y88 (sc-134457, 1:1000 Santa Cruz BioTech, USA); rabbit anti-Prospero (1:1000, a gift from Denan Wang), guinea pig anti-Dpn (1:10000, a gift from James B. Skeath), rabbit anti-DE-cadherin (sc-33743, 1:1000 Santa Cruz BioTech, USA).

    Techniques: Modification, Staining, Marker, Mutagenesis