Journal: Nucleic Acids Research

Article Title: Inhibition of HMGA2 binding to AT-rich DNA by its negatively charged C-terminus

doi: 10.1093/nar/gkaf035

Figure Lengend Snippet: Amino acid sequences of HMGA2, ATHP1, ATHP2, ATHP3, and CTMP, and the DNA sequence of the AT-rich DNA oligomer FL814. AT-hooks and the C-terminal motif are highlighted. A cysteine residue is added to the N-terminus of CTMP for fluorescence labeling. The ATHP2 for florescence labeling also contains a cysteine residue and another four amino acid residues in the N-terminus. For molecular modeling and mass spectrometry studies, ATHP2 only contains the AT hook (KRPRGRPRK).

Article Snippet: All peptides including the CTMP (the C-terminal peptide, CETEETSSQESAEED, Fig. ), the positively charged AT-hook 2 peptide (CEPSPKRPRGRPK, Fig. ), and the positively charged AT-hook 3 peptide (KRPRGRPRKW, Fig. ) were custom-synthesized by Advanced ChemTech, Inc. Biotin-labeled DNA oligomer FL814 (Fig. ) carrying a specific SELEX binding site of HMGA2 [ ] was purchased from Eurofins MWG Operon, Inc.

Techniques: Sequencing, Residue, Fluorescence, Labeling, Mass Spectrometry