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(a) Study design comparing propofol (PRO) and ketamine/dexmedetomidine (K/D) anesthesia in 10-week-old Landrace pigs (n=5 per group), including cisterna magna (CM) cannulation, tracer infusion, and <t>dynamic</t> <t>SPECT/CT</t> acquisition.Experimental timeline and procedural workflow from animal preparation to imaging and post-scan gamma counting. (b) CM cannulation steps. (c) Representative SPECT/CT images showing tracer distribution over time (0, 30, 60, and 180 min) in axial, coronal, and sagittal views, illustrating tracer entry from the CM and subsequent brain-wide dispersion. A red cross in the middle of the brain marks the location of the SPECT/CT slices shown. (d-j) Time-activity curves (TACs, % injected dose) and corresponding area under the curve (AUC) analyses for CSF and brain regions, including CM (d), intracranial space (e), nasal cavity (f), olfactory bulb (g), dorsal striatum (PRO group n=3) (h), and thalamus (PRO group n=4) (i), and prefrontal cortex (j) comparing PRO (red) and K/D (blue) groups. Significant stars are, * if p-value < 0.05, ** p-value is <0.01,***p-value <0.001. (k-l) Additional systemic tracer distribution and clearance dynamics of radioactivity TACs and AUC for blood (k), and cumulative urinary tracer excretion over time (l).
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(a) Study design comparing propofol (PRO) and ketamine/dexmedetomidine (K/D) anesthesia in 10-week-old Landrace pigs (n=5 per group), including cisterna magna (CM) cannulation, tracer infusion, and <t>dynamic</t> <t>SPECT/CT</t> acquisition.Experimental timeline and procedural workflow from animal preparation to imaging and post-scan gamma counting. (b) CM cannulation steps. (c) Representative SPECT/CT images showing tracer distribution over time (0, 30, 60, and 180 min) in axial, coronal, and sagittal views, illustrating tracer entry from the CM and subsequent brain-wide dispersion. A red cross in the middle of the brain marks the location of the SPECT/CT slices shown. (d-j) Time-activity curves (TACs, % injected dose) and corresponding area under the curve (AUC) analyses for CSF and brain regions, including CM (d), intracranial space (e), nasal cavity (f), olfactory bulb (g), dorsal striatum (PRO group n=3) (h), and thalamus (PRO group n=4) (i), and prefrontal cortex (j) comparing PRO (red) and K/D (blue) groups. Significant stars are, * if p-value < 0.05, ** p-value is <0.01,***p-value <0.001. (k-l) Additional systemic tracer distribution and clearance dynamics of radioactivity TACs and AUC for blood (k), and cumulative urinary tracer excretion over time (l).
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(a) Study design comparing propofol (PRO) and ketamine/dexmedetomidine (K/D) anesthesia in 10-week-old Landrace pigs (n=5 per group), including cisterna magna (CM) cannulation, tracer infusion, and <t>dynamic</t> <t>SPECT/CT</t> acquisition.Experimental timeline and procedural workflow from animal preparation to imaging and post-scan gamma counting. (b) CM cannulation steps. (c) Representative SPECT/CT images showing tracer distribution over time (0, 30, 60, and 180 min) in axial, coronal, and sagittal views, illustrating tracer entry from the CM and subsequent brain-wide dispersion. A red cross in the middle of the brain marks the location of the SPECT/CT slices shown. (d-j) Time-activity curves (TACs, % injected dose) and corresponding area under the curve (AUC) analyses for CSF and brain regions, including CM (d), intracranial space (e), nasal cavity (f), olfactory bulb (g), dorsal striatum (PRO group n=3) (h), and thalamus (PRO group n=4) (i), and prefrontal cortex (j) comparing PRO (red) and K/D (blue) groups. Significant stars are, * if p-value < 0.05, ** p-value is <0.01,***p-value <0.001. (k-l) Additional systemic tracer distribution and clearance dynamics of radioactivity TACs and AUC for blood (k), and cumulative urinary tracer excretion over time (l).
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Image Search Results


(a) Study design comparing propofol (PRO) and ketamine/dexmedetomidine (K/D) anesthesia in 10-week-old Landrace pigs (n=5 per group), including cisterna magna (CM) cannulation, tracer infusion, and dynamic SPECT/CT acquisition.Experimental timeline and procedural workflow from animal preparation to imaging and post-scan gamma counting. (b) CM cannulation steps. (c) Representative SPECT/CT images showing tracer distribution over time (0, 30, 60, and 180 min) in axial, coronal, and sagittal views, illustrating tracer entry from the CM and subsequent brain-wide dispersion. A red cross in the middle of the brain marks the location of the SPECT/CT slices shown. (d-j) Time-activity curves (TACs, % injected dose) and corresponding area under the curve (AUC) analyses for CSF and brain regions, including CM (d), intracranial space (e), nasal cavity (f), olfactory bulb (g), dorsal striatum (PRO group n=3) (h), and thalamus (PRO group n=4) (i), and prefrontal cortex (j) comparing PRO (red) and K/D (blue) groups. Significant stars are, * if p-value < 0.05, ** p-value is <0.01,***p-value <0.001. (k-l) Additional systemic tracer distribution and clearance dynamics of radioactivity TACs and AUC for blood (k), and cumulative urinary tracer excretion over time (l).

Journal: bioRxiv

Article Title: Scaling of anesthesia-dependent cerebrospinal fluid dynamics across rat and pig brains

doi: 10.64898/2026.07.10.737206

Figure Lengend Snippet: (a) Study design comparing propofol (PRO) and ketamine/dexmedetomidine (K/D) anesthesia in 10-week-old Landrace pigs (n=5 per group), including cisterna magna (CM) cannulation, tracer infusion, and dynamic SPECT/CT acquisition.Experimental timeline and procedural workflow from animal preparation to imaging and post-scan gamma counting. (b) CM cannulation steps. (c) Representative SPECT/CT images showing tracer distribution over time (0, 30, 60, and 180 min) in axial, coronal, and sagittal views, illustrating tracer entry from the CM and subsequent brain-wide dispersion. A red cross in the middle of the brain marks the location of the SPECT/CT slices shown. (d-j) Time-activity curves (TACs, % injected dose) and corresponding area under the curve (AUC) analyses for CSF and brain regions, including CM (d), intracranial space (e), nasal cavity (f), olfactory bulb (g), dorsal striatum (PRO group n=3) (h), and thalamus (PRO group n=4) (i), and prefrontal cortex (j) comparing PRO (red) and K/D (blue) groups. Significant stars are, * if p-value < 0.05, ** p-value is <0.01,***p-value <0.001. (k-l) Additional systemic tracer distribution and clearance dynamics of radioactivity TACs and AUC for blood (k), and cumulative urinary tracer excretion over time (l).

Article Snippet: Dynamic whole-body SPECT/CT scanning on rats in prone position was performed with the Vector4CT (MILabs, Utrecht, Netherlands) system.

Techniques: Single Photon Emission Computed Tomography, Imaging, Dispersion, Activity Assay, Injection, Olfactory, IF-P, Radioactivity

(a) Study design comparing propofol (PRO), ketamine/dexmedetomidine (K/D) and isoflurante (ISO) anesthesia in 10-weeks old rats, including cisterna magna (CM) cannulation, tracer infusion, and dynamic SPECT/CT acquisition. Experimental timeline and procedural workflow from animal preparation to imaging. (b) and (c) Representative SPECT/CT images showing tracer distribution over time. (i-p) Time-activity curves (TACs, % injected dose) shown individually for each rat in thin lines plus the mean in a thick line and coloured by anesthesia group, and their corresponding area under the curve (AUC) analyses for different ROIs, including CM (i), intracranial space (j), field of view (k), spine (l), striatum (m), and thalamus (n), comparing PRO (red) and K/D (blue) groups. No value is given if p-value >0.01; if p-value is <0.05 = “*”; if p-value is < 0.01 = “**”; and if p-value is <0.005 = “***”. (o-p) Additional systemic tracer distribution and clearance dynamics of radioactivity TACs and AUC for kidneys (o), and cumulative urinary tracer excretion over time (p).

Journal: bioRxiv

Article Title: Scaling of anesthesia-dependent cerebrospinal fluid dynamics across rat and pig brains

doi: 10.64898/2026.07.10.737206

Figure Lengend Snippet: (a) Study design comparing propofol (PRO), ketamine/dexmedetomidine (K/D) and isoflurante (ISO) anesthesia in 10-weeks old rats, including cisterna magna (CM) cannulation, tracer infusion, and dynamic SPECT/CT acquisition. Experimental timeline and procedural workflow from animal preparation to imaging. (b) and (c) Representative SPECT/CT images showing tracer distribution over time. (i-p) Time-activity curves (TACs, % injected dose) shown individually for each rat in thin lines plus the mean in a thick line and coloured by anesthesia group, and their corresponding area under the curve (AUC) analyses for different ROIs, including CM (i), intracranial space (j), field of view (k), spine (l), striatum (m), and thalamus (n), comparing PRO (red) and K/D (blue) groups. No value is given if p-value >0.01; if p-value is <0.05 = “*”; if p-value is < 0.01 = “**”; and if p-value is <0.005 = “***”. (o-p) Additional systemic tracer distribution and clearance dynamics of radioactivity TACs and AUC for kidneys (o), and cumulative urinary tracer excretion over time (p).

Article Snippet: Dynamic whole-body SPECT/CT scanning on rats in prone position was performed with the Vector4CT (MILabs, Utrecht, Netherlands) system.

Techniques: Single Photon Emission Computed Tomography, Imaging, Activity Assay, Injection, IF-P, Radioactivity