jena bioscience jbscreen classic  (Jena Bioscience)


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    Name:
    JBScreen Classic 4
    Description:

    Catalog Number:
    CS-104L
    Price:
    149.0
    Category:
    Crystallography
    Size:
    24 solutions
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    Structured Review

    Jena Bioscience jena bioscience jbscreen classic
    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 <t>(Jena</t> Bioscience <t>JBScreen</t> Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    https://www.bioz.com/result/jena bioscience jbscreen classic/product/Jena Bioscience
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jena bioscience jbscreen classic - by Bioz Stars, 2021-06
    96/100 stars

    Images

    1) Product Images from "Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state"

    Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2018.09.005

    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.
    Figure Legend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Techniques Used:

    Related Articles

    Crystallization Assay:

    Article Title: Cloning, expression, purification, crystallization and X-ray crystallographic analysis of CofB, the minor pilin subunit of CFA/III from human enterotoxigenic Escherichia coli
    Article Snippet: The E. coli cells were cultured in Luria–Bertani (LB) medium in the presence of kanamycin (20 µg ml−1 ) and chloramphenicol (10 µg ml−1 ) for selection until the culture reached an optimal density at 660 nm and 310 K of ∼0.60. .. Initial crystallization conditions were screened by the sitting-drop vapour-diffusion method at 277 and 293 K using commercially available screening kits, including Crystal Screen HT, Index HT, SaltRx, Crystal Screen Cryo and Crystal Screen Cryo 2 (Hampton Research, Aliso Viejo, California, USA), JBScreen Classic I + II and JBScreen Cryo (Jena BioScience, Germany), NR-LBD, Clear Strategy I and II, Heavy + Light and Pact HT (Molecular Dimensions, Altamonte Springs, Florida, USA), Wizard Screen 1 and 2 and Wizard Cryo 1 and 2 (Emerald Bio, Bainbridge Island, Washington, USA). .. Crystallization drops were prepared by mixing 1 µl protein solution and 1 µl reservoir solution and were equilibrated against 80 µl reservoir solution.

    Article Title: Expression, purification and X-ray crystallographic analysis of the Helicobacter pylori blood group antigen-binding adhesin BabA
    Article Snippet: Escherichia coli TOP10 cells were transformed with the pASK-HR3 or pASK-HR4 constructs, grown on lysogeny broth (LB) supplemented with 100 mg ml−1 ampicillin until an OD600 of 0.7 was reached and induced overnight at 293 K with 0.2 mg ml−1 anhydrotetracycline. .. Crystallization of BabA1–460 , BabA25–460 and its complex with Nb-ER19 was first screened at 293 K by the sitting-drop vapour-diffusion method with commercial sparse-matrix crystallization screening kits [SaltRx, SaltRx 2, Grid Screen Salt HT, PEGRx 2, Index HT (Hampton Research), JBScreen Basic, JBScreen Classic, JBScreen PEG/Salt (Jena Bioscience) and PACT premier HT-96, JCSG- plus HT-96 and ProPlex HT-96 (Molecular Dimensions)] in 96-well MRC plates. .. Each crystallization drop was prepared by mixing 400 nl protein solution and 400 nl reservoir solution (a 1:1 ratio) and equilibrating against 50 µl reservoir solution.

    Article Title: Crystallization and preliminary X-ray diffraction analysis of the protease from Southampton norovirus complexed with a Michael acceptor inhibitor
    Article Snippet: Further confirmation and accurate assessment of MAPI binding was accomplished by mass spectrometry, which revealed a single major peak of molecular weight 20 045 corresponding to one molecule of protease covalently linked to one molecule of the inhibitor. .. Further screening for crystallization conditions with the enzyme–inhibitor complex at a concentration of 15 mg ml−1 yielded large rhomboid crystals in 25%( w / v ) PEG 5000 MME, 100 m M Tris–HCl pH 8.5, 200 m M lithium sulfate (Jena Bioscience JBScreen Classic 4 condition A1). ..

    Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state
    Article Snippet: Next, the protein was centrifuged at 21,000 g for 10 min at 10 °C to remove any precipitate generated at the activation step. .. Initial crystallization trials were performed at 5.3 mg ml−1 in 96-well sitting-drop vapour-diffusion Greiner 609120 plates (Monroe, North Carolina, USA) using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and the following crystallization kits: Jena Bioscience JBScreen Classic and Pentaerythritol (Jena, Germany), and Hampton Research Crystal Screen, Crystal Screen 2, PEG/Ion, PEG/Ion 2, PEGRx 1 and PEGRx 2 (Aliso Viejo, California, USA). ..

    Article Title: Expression, purification, crystallization and preliminary crystallographic analysis of an endo-1,5-?-l-arabinanase from hyperthermophilic Thermotoga petrophila
    Article Snippet: Crystallization was performed by the sitting-drop vapour-diffusion method using a Cartesian HoneyBee 963 system (Genomic Solutions). .. 544 conditions from commercially available crystallization kits were tested, including Crystal Screens I and II (Hampton Research) and JBScreen Classic and Basic kits (Jena Bioscience). .. Typically, 0.5 µl drops of protein solution (25 m M Tris–HCl buffer pH 7.5) at a concentration of 30 mg ml−1 were mixed with an equal volume of the screening solution and equilibrated over a reservoir containing 0.3 ml of the latter solution.

    Article Title: Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster
    Article Snippet: The Superdex 200 10/300 column was calibrated using a Gel Filtration Calibration Kit LMW (GE Healthcare). .. Initial crystallization screening was performed at 293 K by the sitting-drop vapour-diffusion method in a 96-well MRC Crystallization Plate (Hampton Research) using screening kits including Crystal Screen, Crystal Screen 2, PEG/Ion, Natrix, Index (Hampton Research), PACT, JCSG+ (Qiagen), MemGold (Molecular Dimensions), Wizard (Emerald BioSystems), JBScreen Classic and JBScreen Membrane (Jena Bioscience). .. Crystallization drops were prepared by mixing 100 nl protein solution and 100 nl reservoir solution using a Mosquito crystallization robot (TTP LabTech).

    Concentration Assay:

    Article Title: Crystallization and preliminary X-ray diffraction analysis of the protease from Southampton norovirus complexed with a Michael acceptor inhibitor
    Article Snippet: Further confirmation and accurate assessment of MAPI binding was accomplished by mass spectrometry, which revealed a single major peak of molecular weight 20 045 corresponding to one molecule of protease covalently linked to one molecule of the inhibitor. .. Further screening for crystallization conditions with the enzyme–inhibitor complex at a concentration of 15 mg ml−1 yielded large rhomboid crystals in 25%( w / v ) PEG 5000 MME, 100 m M Tris–HCl pH 8.5, 200 m M lithium sulfate (Jena Bioscience JBScreen Classic 4 condition A1). ..

    High Throughput Screening Assay:

    Article Title: Structural characterization of the essential cell division protein FtsE and its interaction with FtsX in Streptococcus pneumoniae
    Article Snippet: Concentration of purified FtsE was determined by absorbance at 280 nm using an extinction coefficient of ε = 15,930 M−1 cm−1 calculated by the ExPASy ProtParam program ( ). .. FtsE crystallizationCrystallization screenings were performed by high-throughput techniques in a NanoDrop robot and Innovadyne SD-2 microplates (Innovadyne Technologies Inc.), screening PACT Suite and JCSG Suite (Qiagen), JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen, Crystal Screen 2 and Index HT (Hampton Research). .. The conditions that produced crystals were optimized by sitting-drop vapor-diffusion method at 291 K by mixing 1 µL of protein solution and 1 µL of precipitant solution, equilibrated against 150 µL of precipitant solution in the reservoir chamber.

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  • 96
    Jena Bioscience jena bioscience jbscreen classic
    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 <t>(Jena</t> Bioscience <t>JBScreen</t> Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.
    Jena Bioscience Jbscreen Classic, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jena bioscience jbscreen classic/product/Jena Bioscience
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jena bioscience jbscreen classic - by Bioz Stars, 2021-06
    96/100 stars
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    LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

    doi: 10.1016/j.bbrep.2018.09.005

    Figure Lengend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

    Article Snippet: Initial crystallization trials were performed at 5.3 mg ml−1 in 96-well sitting-drop vapour-diffusion Greiner 609120 plates (Monroe, North Carolina, USA) using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and the following crystallization kits: Jena Bioscience JBScreen Classic and Pentaerythritol (Jena, Germany), and Hampton Research Crystal Screen, Crystal Screen 2, PEG/Ion, PEG/Ion 2, PEGRx 1 and PEGRx 2 (Aliso Viejo, California, USA).

    Techniques: