Journal: Materials Today Bio
Article Title: Sodium butyrate enhances sorafenib-induced ferroptosis and immunogenic cell death by modulating IRF2-Oasl2-cGAS pathway in colorectal cancer
doi: 10.1016/j.mtbio.2025.102498
Figure Lengend Snippet: Activation of antitumor immunity by LipNaB@Sor induced ICD. (A–C) Representative immunofluorescence images demonstrating the presence of HMGB1, ecto-CRT, and P-IRF3. Scale bar = 50 μm. (D) Immunoblot analysis was performed to identify proteins involved in the IRF2-Oasl2-cGAS signaling pathway in subcutaneous tumors from various treatment groups. Representative flow cytometry plots and quantitative analysis of indicated markers in the subcutaneous tumor model on day 14. (E–G) Expression of MHC II, CD80, and CD86 on DCs. (H) Proportion of CD8 + T cells. (I, J) Frequency of IFNγ + and GZMB + cells among CD8 + T cells. Data are from n = 5 mice per group. Statistical significance is indicated as follows: ns, P > 0.05; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Article Snippet: Cells were then incubated overnight at 4 °C with primary antibodies against CRT (Proteintech, 10292-1-AP), HMGB1 (MCE, HY- P80183 ), γH2AX (Abcam, ab81299), OASL (Abcam, ab229136), and cGAS (Abcam, ab302617).
Techniques: Activation Assay, Immunofluorescence, Western Blot, Flow Cytometry, Expressing