Journal: Experimental and Therapeutic Medicine

Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

doi: 10.3892/etm.2016.3588

Figure Lengend Snippet: Expression of CRMP-4 in colorectal cancer and control tissues. (A) Western blot analysis of the protein expression of CRMP-4 in tissues from three patients and three healthy individuals, with GAPDH used as a loading control. Data obtained from three independent experiments were normalized against GAPDH and are expressed as the mean ± standard deviation, as analyzed with one-way analysis of variance. (B) Immunohistochemical analysis of the expression of CRMP-4 in tissues (stain, hematoxylin; magnification, ×100). *P<0.05 vs. the N group. CRMP-4, collapsin response mediator protein 4; N: normal colon tissue from healthy persons, A: normal adjacent tissues of colorectal cancer, C: colorectal cancer tissue.

Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Standard Deviation, Immunohistochemical staining, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

doi: 10.3892/etm.2016.3588

Figure Lengend Snippet: Verification of knockdown CRMP-4 expression by lentivirus. (A) SW480 cells infected with lentivirus. (B) Reverse transcription-polymerase chain reaction and western blot analysis. GAPDH was used as an internal control. CRMP-4, collapsin response mediator protein 4; lv-si-CRMP-4, SW480 cells infected with lv-si-CRMP-4 lentivirus; lv-si-scramble, SW480 cells infected with lv-si-scramble lentivirus.

Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Expressing, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

doi: 10.3892/etm.2016.3588

Figure Lengend Snippet: Downregulation of CRMP-4 inhibits the proliferation of SW480 cells. (A) Viability of SW480 cells were evaluated by MTT assay. (B) Proliferation of SW480 cells were evaluated by BrdU incorporation. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4; OD, optical density.

Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: MTT Assay, BrdU Incorporation Assay, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth

doi: 10.3892/etm.2016.3588

Figure Lengend Snippet: Colon tumor sizes in each group. (A) Colon tumor of mice injected with si-scramble cells or si-CRMP-4 cells after 4 weeks of injection. (B) Colon tumor size in each group after lentivirus injection at 1, 2, 3 and 4 weeks, respectively. Data were obtained from three independent experiments and are expressed as the mean ± standard deviation, as analyzed using one-way analysis of variance. *P<0.05 vs. the si-CRMP-4 group at the same time point. CRMP-4, collapsin response mediator protein 4.

Article Snippet: CRMP-4 (cat. no. 4612) and GAPDH (cat. no. 5174) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Injection, Standard Deviation