dermal microvascular endothelial cells  (ATCC)


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    ATCC dermal microvascular endothelial cells
    Dermal Microvascular Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dermal microvascular endothelial cells  (ATCC)


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    ATCC dermal microvascular endothelial cells
    Dermal Microvascular Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dermal microvascular endothelial cells  (ATCC)


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    ATCC dermal microvascular endothelial cells
    Dermal Microvascular Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    htert time atcc crl 4025 human  (ATCC)


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    ATCC htert time atcc crl 4025 human
    Htert Time Atcc Crl 4025 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human cell line time  (ATCC)


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    ATCC human cell line time
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Human Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p"

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    Journal: Cells

    doi: 10.3390/cells10112843

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Figure Legend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).

    Techniques Used: Next-Generation Sequencing

    The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.
    Figure Legend Snippet: The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.

    Techniques Used: Cell Culture, Standard Deviation

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.
    Figure Legend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.

    Techniques Used: Isolation, Digital PCR, Expressing, Software

    time crl 4025 cell lines  (ATCC)


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    ATCC time crl 4025 cell lines
    Time Crl 4025 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    time cell line atcc crl 4025 authentication  (ATCC)


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    ATCC time cell line atcc crl 4025 authentication
    Time Cell Line Atcc Crl 4025 Authentication, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    crl 4025  (ATCC)


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    ATCC crl 4025
    Crl 4025, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human telomerase htert  (ATCC)


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    ATCC human telomerase htert
    Human Telomerase Htert, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin  (ATCC)


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    ATCC human foreskin
    Human Foreskin, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    microvascular endothelial cell  (ATCC)


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    ATCC microvascular endothelial cell
    TAK1 deletion reduced the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human <t>microvascular</t> endothelial cells. qPCR results revealed that TAK1 deletion suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in TIMEs stimulated with IL1 β for 24 hours (n = 4; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Microvascular Endothelial Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "TAK1 blockade as a therapy for retinal neovascularization"

    Article Title: TAK1 blockade as a therapy for retinal neovascularization

    Journal: bioRxiv

    doi: 10.1101/2021.01.29.428701

    TAK1 deletion reduced the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human microvascular endothelial cells. qPCR results revealed that TAK1 deletion suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in TIMEs stimulated with IL1 β for 24 hours (n = 4; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: TAK1 deletion reduced the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human microvascular endothelial cells. qPCR results revealed that TAK1 deletion suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in TIMEs stimulated with IL1 β for 24 hours (n = 4; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Expressing

    TAK1 knockdown by siRNA inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human primary retinal microvascular endothelial cells (HRMECs). qPCR results revealed that TAK1 knockdown by siRNA targeting TAK1 (siTAK1) suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in HRMECs stimulated with TNFα for 24 hours (n = 3). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: TAK1 knockdown by siRNA inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human primary retinal microvascular endothelial cells (HRMECs). qPCR results revealed that TAK1 knockdown by siRNA targeting TAK1 (siTAK1) suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in HRMECs stimulated with TNFα for 24 hours (n = 3). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Expressing

    TAK1 inhibition by 5Z-7-oxozeaenol inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in primary human retinal microvascular endothelial cells (HRMECs). qPCR results revealed that pharmacological inhibition of TAK1 by 5Z-7-oxozeaenol (Oxo) suppressed ICAM-1 , PTGS2 , CXCL8 and VEGFA in HRMECs stimulated with TNFα in a dose-dependent manner (n = 3; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: TAK1 inhibition by 5Z-7-oxozeaenol inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in primary human retinal microvascular endothelial cells (HRMECs). qPCR results revealed that pharmacological inhibition of TAK1 by 5Z-7-oxozeaenol (Oxo) suppressed ICAM-1 , PTGS2 , CXCL8 and VEGFA in HRMECs stimulated with TNFα in a dose-dependent manner (n = 3; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Inhibition, Expressing

    (A) Experimental protocol for 5Z-7-oxozeaenol treatment in the OIR rat model. A single intravitreal injection of vehicle, low (18 ng) or high (90 ng) dose of 5Z-7-oxozeaenol was given to Sprague-Dawley OIR rats at P14, and retinae were collected for qPCR and immunostaining at P16 and P18. (B) Intravitreal injection of 5Z-7-oxozeaenol in OIR rats at P14 inhibited TNFα , VEGFA and ICAM-1 gene expression assessed using qPCR (n = 5-7). (C) Representative images of microglial adhesion to the vascular surface in a retina from a control rat and OIR rats that had a single intravitreal injection of vehicle, low or high dose of 5Z-7-oxozeaenol (Oxo). Vessels were visualized with isolectin GS-IB4 staining (green), and distribution of microglia was immunolabeled with Iba-1 (red). Scale bar: 50 µm. (D) Quantitative analysis of microglial number relative to retinal vessel area in vehicle or 5Z-7-oxozeaenol treated OIR rats (n = 7-10; 10 images from each retina were shown as separated dot plot). (E) Schematic diagram for the role of TAK1 signaling in microvascular endothelial cells. Proinflammatory cytokines such as TNFα can activate TAK1 in microvascular endothelial cells, and TAK1/TAB1/TAB2-3 complex can lead to activation of NFκB and MAPKs/AP-1 cascades. NFκB and MAPKs/AP-1 induce expression of “cytokines” and “adhesion proteins”, thereby triggering the “inflammatory cycle” and “leukostasis”, and leading to retinal neovascularization. Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: (A) Experimental protocol for 5Z-7-oxozeaenol treatment in the OIR rat model. A single intravitreal injection of vehicle, low (18 ng) or high (90 ng) dose of 5Z-7-oxozeaenol was given to Sprague-Dawley OIR rats at P14, and retinae were collected for qPCR and immunostaining at P16 and P18. (B) Intravitreal injection of 5Z-7-oxozeaenol in OIR rats at P14 inhibited TNFα , VEGFA and ICAM-1 gene expression assessed using qPCR (n = 5-7). (C) Representative images of microglial adhesion to the vascular surface in a retina from a control rat and OIR rats that had a single intravitreal injection of vehicle, low or high dose of 5Z-7-oxozeaenol (Oxo). Vessels were visualized with isolectin GS-IB4 staining (green), and distribution of microglia was immunolabeled with Iba-1 (red). Scale bar: 50 µm. (D) Quantitative analysis of microglial number relative to retinal vessel area in vehicle or 5Z-7-oxozeaenol treated OIR rats (n = 7-10; 10 images from each retina were shown as separated dot plot). (E) Schematic diagram for the role of TAK1 signaling in microvascular endothelial cells. Proinflammatory cytokines such as TNFα can activate TAK1 in microvascular endothelial cells, and TAK1/TAB1/TAB2-3 complex can lead to activation of NFκB and MAPKs/AP-1 cascades. NFκB and MAPKs/AP-1 induce expression of “cytokines” and “adhesion proteins”, thereby triggering the “inflammatory cycle” and “leukostasis”, and leading to retinal neovascularization. Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Injection, Immunostaining, Expressing, Staining, Immunolabeling, Activation Assay

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    ATCC dermal microvascular endothelial cells
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    ATCC human cell line time
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Human Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC time crl 4025 cell lines
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Time Crl 4025 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC time cell line atcc crl 4025 authentication
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Time Cell Line Atcc Crl 4025 Authentication, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC crl 4025
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Crl 4025, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC human telomerase htert
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Human Telomerase Htert, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC human foreskin
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Human Foreskin, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC microvascular endothelial cell
    TAK1 deletion reduced the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human <t>microvascular</t> endothelial cells. qPCR results revealed that TAK1 deletion suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in TIMEs stimulated with IL1 β for 24 hours (n = 4; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Microvascular Endothelial Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).

    Journal: Cells

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    doi: 10.3390/cells10112843

    Figure Lengend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).

    Article Snippet: The human cell line TIME (ATCC ® number CRL-4025) cultured at 37 °C and 5% CO 2 in a humid atmosphere served as an in vitro model for microvascular endothelial cells.

    Techniques: Next-Generation Sequencing

    The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.

    Journal: Cells

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    doi: 10.3390/cells10112843

    Figure Lengend Snippet: The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.

    Article Snippet: The human cell line TIME (ATCC ® number CRL-4025) cultured at 37 °C and 5% CO 2 in a humid atmosphere served as an in vitro model for microvascular endothelial cells.

    Techniques: Cell Culture, Standard Deviation

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.

    Journal: Cells

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    doi: 10.3390/cells10112843

    Figure Lengend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.

    Article Snippet: The human cell line TIME (ATCC ® number CRL-4025) cultured at 37 °C and 5% CO 2 in a humid atmosphere served as an in vitro model for microvascular endothelial cells.

    Techniques: Isolation, Digital PCR, Expressing, Software

    TAK1 deletion reduced the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human microvascular endothelial cells. qPCR results revealed that TAK1 deletion suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in TIMEs stimulated with IL1 β for 24 hours (n = 4; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: TAK1 blockade as a therapy for retinal neovascularization

    doi: 10.1101/2021.01.29.428701

    Figure Lengend Snippet: TAK1 deletion reduced the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human microvascular endothelial cells. qPCR results revealed that TAK1 deletion suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in TIMEs stimulated with IL1 β for 24 hours (n = 4; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Telomerase-immortalized human microvascular endothelial cell (TIME; CRL-4025™) and primary human retinal microvascular endothelial cell (HRMEC; ACBRI 181V) were purchased from American Type Culture Collection (ATCC; Manassas, VA) and Cell Systems (Kirkland, WA).

    Techniques: Expressing

    TAK1 knockdown by siRNA inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human primary retinal microvascular endothelial cells (HRMECs). qPCR results revealed that TAK1 knockdown by siRNA targeting TAK1 (siTAK1) suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in HRMECs stimulated with TNFα for 24 hours (n = 3). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: TAK1 blockade as a therapy for retinal neovascularization

    doi: 10.1101/2021.01.29.428701

    Figure Lengend Snippet: TAK1 knockdown by siRNA inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in human primary retinal microvascular endothelial cells (HRMECs). qPCR results revealed that TAK1 knockdown by siRNA targeting TAK1 (siTAK1) suppressed the expression of selected cytokines ( CXCL8 and VEGFA) and adhesion molecules ( ICAM-1) in HRMECs stimulated with TNFα for 24 hours (n = 3). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Telomerase-immortalized human microvascular endothelial cell (TIME; CRL-4025™) and primary human retinal microvascular endothelial cell (HRMEC; ACBRI 181V) were purchased from American Type Culture Collection (ATCC; Manassas, VA) and Cell Systems (Kirkland, WA).

    Techniques: Expressing

    TAK1 inhibition by 5Z-7-oxozeaenol inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in primary human retinal microvascular endothelial cells (HRMECs). qPCR results revealed that pharmacological inhibition of TAK1 by 5Z-7-oxozeaenol (Oxo) suppressed ICAM-1 , PTGS2 , CXCL8 and VEGFA in HRMECs stimulated with TNFα in a dose-dependent manner (n = 3; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: TAK1 blockade as a therapy for retinal neovascularization

    doi: 10.1101/2021.01.29.428701

    Figure Lengend Snippet: TAK1 inhibition by 5Z-7-oxozeaenol inhibited the expression of TAK1, ICAM-1, CXCL8 and VEGF genes in primary human retinal microvascular endothelial cells (HRMECs). qPCR results revealed that pharmacological inhibition of TAK1 by 5Z-7-oxozeaenol (Oxo) suppressed ICAM-1 , PTGS2 , CXCL8 and VEGFA in HRMECs stimulated with TNFα in a dose-dependent manner (n = 3; technical duplicate from each biological repeat was shown as separated dot plot). Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Telomerase-immortalized human microvascular endothelial cell (TIME; CRL-4025™) and primary human retinal microvascular endothelial cell (HRMEC; ACBRI 181V) were purchased from American Type Culture Collection (ATCC; Manassas, VA) and Cell Systems (Kirkland, WA).

    Techniques: Inhibition, Expressing

    (A) Experimental protocol for 5Z-7-oxozeaenol treatment in the OIR rat model. A single intravitreal injection of vehicle, low (18 ng) or high (90 ng) dose of 5Z-7-oxozeaenol was given to Sprague-Dawley OIR rats at P14, and retinae were collected for qPCR and immunostaining at P16 and P18. (B) Intravitreal injection of 5Z-7-oxozeaenol in OIR rats at P14 inhibited TNFα , VEGFA and ICAM-1 gene expression assessed using qPCR (n = 5-7). (C) Representative images of microglial adhesion to the vascular surface in a retina from a control rat and OIR rats that had a single intravitreal injection of vehicle, low or high dose of 5Z-7-oxozeaenol (Oxo). Vessels were visualized with isolectin GS-IB4 staining (green), and distribution of microglia was immunolabeled with Iba-1 (red). Scale bar: 50 µm. (D) Quantitative analysis of microglial number relative to retinal vessel area in vehicle or 5Z-7-oxozeaenol treated OIR rats (n = 7-10; 10 images from each retina were shown as separated dot plot). (E) Schematic diagram for the role of TAK1 signaling in microvascular endothelial cells. Proinflammatory cytokines such as TNFα can activate TAK1 in microvascular endothelial cells, and TAK1/TAB1/TAB2-3 complex can lead to activation of NFκB and MAPKs/AP-1 cascades. NFκB and MAPKs/AP-1 induce expression of “cytokines” and “adhesion proteins”, thereby triggering the “inflammatory cycle” and “leukostasis”, and leading to retinal neovascularization. Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: TAK1 blockade as a therapy for retinal neovascularization

    doi: 10.1101/2021.01.29.428701

    Figure Lengend Snippet: (A) Experimental protocol for 5Z-7-oxozeaenol treatment in the OIR rat model. A single intravitreal injection of vehicle, low (18 ng) or high (90 ng) dose of 5Z-7-oxozeaenol was given to Sprague-Dawley OIR rats at P14, and retinae were collected for qPCR and immunostaining at P16 and P18. (B) Intravitreal injection of 5Z-7-oxozeaenol in OIR rats at P14 inhibited TNFα , VEGFA and ICAM-1 gene expression assessed using qPCR (n = 5-7). (C) Representative images of microglial adhesion to the vascular surface in a retina from a control rat and OIR rats that had a single intravitreal injection of vehicle, low or high dose of 5Z-7-oxozeaenol (Oxo). Vessels were visualized with isolectin GS-IB4 staining (green), and distribution of microglia was immunolabeled with Iba-1 (red). Scale bar: 50 µm. (D) Quantitative analysis of microglial number relative to retinal vessel area in vehicle or 5Z-7-oxozeaenol treated OIR rats (n = 7-10; 10 images from each retina were shown as separated dot plot). (E) Schematic diagram for the role of TAK1 signaling in microvascular endothelial cells. Proinflammatory cytokines such as TNFα can activate TAK1 in microvascular endothelial cells, and TAK1/TAB1/TAB2-3 complex can lead to activation of NFκB and MAPKs/AP-1 cascades. NFκB and MAPKs/AP-1 induce expression of “cytokines” and “adhesion proteins”, thereby triggering the “inflammatory cycle” and “leukostasis”, and leading to retinal neovascularization. Group data are shown as means ± SEM. Statistical analysis was undertaken with one-way ANOVA and Tukey’s multiple comparison test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Telomerase-immortalized human microvascular endothelial cell (TIME; CRL-4025™) and primary human retinal microvascular endothelial cell (HRMEC; ACBRI 181V) were purchased from American Type Culture Collection (ATCC; Manassas, VA) and Cell Systems (Kirkland, WA).

    Techniques: Injection, Immunostaining, Expressing, Staining, Immunolabeling, Activation Assay