crispr cas9 skeleton vector  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    New England Biolabs crispr cas9 skeleton vector
    <t>CRISPR/Cas9-based</t> deletion system in zebrafish. ( A ) Schematic illustration of the constructs of the CRISPR/Cas9-based vector, including the gfap promoter-driving Cas9, mCherry reporter, and U6-driving specific gRNA. ( B ) Strategy of the generation of the transgenic zebrafish lines. ( C ) F 1 transgenic fish generated by mosaic mCherry-positive fish outcrossed with wild-type fish. A subset of the transgenic offspring was identified by mCherry-positive astrocytes. Scale bar = 1 mm. ( D ) The colocalization of mCherry-labelled Cas9 and GFP-labelled endogenous Gfap in 60 hpf offspring from the cross of gfap WT and Tg ( gfap : GFP ) transgenic fish. Scale bars = 500 μm. ( E – J ) Co-localization of mCherry fluorescent signals (red) and endogenous Gfap detected by anti-Gfap antibody (FITC; green) in the brain and retina ( E – G ) or spinal cord ( H – J ) tissue. Scale bars = 200 μm. ( K ) Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 48 hpf embryos injected with Tol2 mRNA and gfap : Cas9-T2A-mCherry,U6 : gRNA ( null ) vector expressing Cas9 under the control of the gfap promoter. Cas9 expression pattern is governed by the tissue-specificity of gfap promoter (yellow arrowheads). Scale bars = 500 μm.
    Crispr Cas9 Skeleton Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crispr cas9 skeleton vector/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    crispr cas9 skeleton vector - by Bioz Stars, 2022-08
    99/100 stars

    Images

    1) Product Images from "Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish"

    Article Title: Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish

    Journal: Brain

    doi: 10.1093/brain/awaa404

    CRISPR/Cas9-based deletion system in zebrafish. ( A ) Schematic illustration of the constructs of the CRISPR/Cas9-based vector, including the gfap promoter-driving Cas9, mCherry reporter, and U6-driving specific gRNA. ( B ) Strategy of the generation of the transgenic zebrafish lines. ( C ) F 1 transgenic fish generated by mosaic mCherry-positive fish outcrossed with wild-type fish. A subset of the transgenic offspring was identified by mCherry-positive astrocytes. Scale bar = 1 mm. ( D ) The colocalization of mCherry-labelled Cas9 and GFP-labelled endogenous Gfap in 60 hpf offspring from the cross of gfap WT and Tg ( gfap : GFP ) transgenic fish. Scale bars = 500 μm. ( E – J ) Co-localization of mCherry fluorescent signals (red) and endogenous Gfap detected by anti-Gfap antibody (FITC; green) in the brain and retina ( E – G ) or spinal cord ( H – J ) tissue. Scale bars = 200 μm. ( K ) Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 48 hpf embryos injected with Tol2 mRNA and gfap : Cas9-T2A-mCherry,U6 : gRNA ( null ) vector expressing Cas9 under the control of the gfap promoter. Cas9 expression pattern is governed by the tissue-specificity of gfap promoter (yellow arrowheads). Scale bars = 500 μm.
    Figure Legend Snippet: CRISPR/Cas9-based deletion system in zebrafish. ( A ) Schematic illustration of the constructs of the CRISPR/Cas9-based vector, including the gfap promoter-driving Cas9, mCherry reporter, and U6-driving specific gRNA. ( B ) Strategy of the generation of the transgenic zebrafish lines. ( C ) F 1 transgenic fish generated by mosaic mCherry-positive fish outcrossed with wild-type fish. A subset of the transgenic offspring was identified by mCherry-positive astrocytes. Scale bar = 1 mm. ( D ) The colocalization of mCherry-labelled Cas9 and GFP-labelled endogenous Gfap in 60 hpf offspring from the cross of gfap WT and Tg ( gfap : GFP ) transgenic fish. Scale bars = 500 μm. ( E – J ) Co-localization of mCherry fluorescent signals (red) and endogenous Gfap detected by anti-Gfap antibody (FITC; green) in the brain and retina ( E – G ) or spinal cord ( H – J ) tissue. Scale bars = 200 μm. ( K ) Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 48 hpf embryos injected with Tol2 mRNA and gfap : Cas9-T2A-mCherry,U6 : gRNA ( null ) vector expressing Cas9 under the control of the gfap promoter. Cas9 expression pattern is governed by the tissue-specificity of gfap promoter (yellow arrowheads). Scale bars = 500 μm.

    Techniques Used: CRISPR, Construct, Plasmid Preparation, Transgenic Assay, Fluorescence In Situ Hybridization, Generated, In Situ Hybridization, Injection, Expressing

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs crispr cas9 skeleton vector
    <t>CRISPR/Cas9-based</t> deletion system in zebrafish. ( A ) Schematic illustration of the constructs of the CRISPR/Cas9-based vector, including the gfap promoter-driving Cas9, mCherry reporter, and U6-driving specific gRNA. ( B ) Strategy of the generation of the transgenic zebrafish lines. ( C ) F 1 transgenic fish generated by mosaic mCherry-positive fish outcrossed with wild-type fish. A subset of the transgenic offspring was identified by mCherry-positive astrocytes. Scale bar = 1 mm. ( D ) The colocalization of mCherry-labelled Cas9 and GFP-labelled endogenous Gfap in 60 hpf offspring from the cross of gfap WT and Tg ( gfap : GFP ) transgenic fish. Scale bars = 500 μm. ( E – J ) Co-localization of mCherry fluorescent signals (red) and endogenous Gfap detected by anti-Gfap antibody (FITC; green) in the brain and retina ( E – G ) or spinal cord ( H – J ) tissue. Scale bars = 200 μm. ( K ) Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 48 hpf embryos injected with Tol2 mRNA and gfap : Cas9-T2A-mCherry,U6 : gRNA ( null ) vector expressing Cas9 under the control of the gfap promoter. Cas9 expression pattern is governed by the tissue-specificity of gfap promoter (yellow arrowheads). Scale bars = 500 μm.
    Crispr Cas9 Skeleton Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crispr cas9 skeleton vector/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    crispr cas9 skeleton vector - by Bioz Stars, 2022-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    CRISPR/Cas9-based deletion system in zebrafish. ( A ) Schematic illustration of the constructs of the CRISPR/Cas9-based vector, including the gfap promoter-driving Cas9, mCherry reporter, and U6-driving specific gRNA. ( B ) Strategy of the generation of the transgenic zebrafish lines. ( C ) F 1 transgenic fish generated by mosaic mCherry-positive fish outcrossed with wild-type fish. A subset of the transgenic offspring was identified by mCherry-positive astrocytes. Scale bar = 1 mm. ( D ) The colocalization of mCherry-labelled Cas9 and GFP-labelled endogenous Gfap in 60 hpf offspring from the cross of gfap WT and Tg ( gfap : GFP ) transgenic fish. Scale bars = 500 μm. ( E – J ) Co-localization of mCherry fluorescent signals (red) and endogenous Gfap detected by anti-Gfap antibody (FITC; green) in the brain and retina ( E – G ) or spinal cord ( H – J ) tissue. Scale bars = 200 μm. ( K ) Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 48 hpf embryos injected with Tol2 mRNA and gfap : Cas9-T2A-mCherry,U6 : gRNA ( null ) vector expressing Cas9 under the control of the gfap promoter. Cas9 expression pattern is governed by the tissue-specificity of gfap promoter (yellow arrowheads). Scale bars = 500 μm.

    Journal: Brain

    Article Title: Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish

    doi: 10.1093/brain/awaa404

    Figure Lengend Snippet: CRISPR/Cas9-based deletion system in zebrafish. ( A ) Schematic illustration of the constructs of the CRISPR/Cas9-based vector, including the gfap promoter-driving Cas9, mCherry reporter, and U6-driving specific gRNA. ( B ) Strategy of the generation of the transgenic zebrafish lines. ( C ) F 1 transgenic fish generated by mosaic mCherry-positive fish outcrossed with wild-type fish. A subset of the transgenic offspring was identified by mCherry-positive astrocytes. Scale bar = 1 mm. ( D ) The colocalization of mCherry-labelled Cas9 and GFP-labelled endogenous Gfap in 60 hpf offspring from the cross of gfap WT and Tg ( gfap : GFP ) transgenic fish. Scale bars = 500 μm. ( E – J ) Co-localization of mCherry fluorescent signals (red) and endogenous Gfap detected by anti-Gfap antibody (FITC; green) in the brain and retina ( E – G ) or spinal cord ( H – J ) tissue. Scale bars = 200 μm. ( K ) Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 48 hpf embryos injected with Tol2 mRNA and gfap : Cas9-T2A-mCherry,U6 : gRNA ( null ) vector expressing Cas9 under the control of the gfap promoter. Cas9 expression pattern is governed by the tissue-specificity of gfap promoter (yellow arrowheads). Scale bars = 500 μm.

    Article Snippet: DNA construction and microinjection We cloned the zebrafish U6-3 promoter ( ) from the AB strain, followed by an NheI site, into the CRISPR/Cas9 skeleton vector (Cas9-T2A-mCherry ) from the Tol2 kit ( ) using ClaI and KpnI endonucleases (New England Biolabs).

    Techniques: CRISPR, Construct, Plasmid Preparation, Transgenic Assay, Fluorescence In Situ Hybridization, Generated, In Situ Hybridization, Injection, Expressing