Crispr Cas9 Skeleton Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr cas9 skeleton vector/product/New England Biolabs
Average 99 stars, based on 1 article reviews
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1) Product Images from "Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish"
Article Title: Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish
Figure Legend Snippet: CRISPR/Cas9-based deletion system in zebrafish. ( A ) Schematic illustration of the constructs of the CRISPR/Cas9-based vector, including the gfap promoter-driving Cas9, mCherry reporter, and U6-driving specific gRNA. ( B ) Strategy of the generation of the transgenic zebrafish lines. ( C ) F 1 transgenic fish generated by mosaic mCherry-positive fish outcrossed with wild-type fish. A subset of the transgenic offspring was identified by mCherry-positive astrocytes. Scale bar = 1 mm. ( D ) The colocalization of mCherry-labelled Cas9 and GFP-labelled endogenous Gfap in 60 hpf offspring from the cross of gfap WT and Tg ( gfap : GFP ) transgenic fish. Scale bars = 500 μm. ( E – J ) Co-localization of mCherry fluorescent signals (red) and endogenous Gfap detected by anti-Gfap antibody (FITC; green) in the brain and retina ( E – G ) or spinal cord ( H – J ) tissue. Scale bars = 200 μm. ( K ) Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 48 hpf embryos injected with Tol2 mRNA and gfap : Cas9-T2A-mCherry,U6 : gRNA ( null ) vector expressing Cas9 under the control of the gfap promoter. Cas9 expression pattern is governed by the tissue-specificity of gfap promoter (yellow arrowheads). Scale bars = 500 μm.
Techniques Used: CRISPR, Construct, Plasmid Preparation, Transgenic Assay, Fluorescence In Situ Hybridization, Generated, In Situ Hybridization, Injection, Expressing