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HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .
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HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .
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HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC <t>BAA-493.</t> ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .
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HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC <t>BAA-493.</t> ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .
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HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .
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ATCC lactococcus cremoris subsp cremoris atcc baa 493
HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC <t>BAA-493.</t> ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .
Lactococcus Cremoris Subsp Cremoris Atcc Baa 493, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Article Snippet: Lactococcus cremoris subsp. cremoris ATCC 11602 , 0.02 , 0.51 , 3.92 , + , NA , ATCC.

Techniques:

HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Article Snippet: The plasmalogen species produced by L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, ATCC BAA-493, and Lactococcus sp. #8-3 were classified as type A, and those produced by E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T were classified as type B.

Techniques:

HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Article Snippet: In contrast, the corresponding plasmalogen biosynthesis genes in E. faecalis K-4, L. cremoris ATCC BAA-493, and B. longum subsp. suis DSM 20211 were all encoded by a single gene.

Techniques:

Differences in various plasmalogen synthase enzymes based on AlphaFold3 three-dimensional structure predictions. ( A ) E. faecalis K-4, C. perfringens NH13, ( B ) B. suis DSM20211, and ( C ) L. cremoris ATCC BAA-493. Act1 and Act2, activation domains; Red1 and Red2, reduction/dehydration domains. ( D ) [4Fe-4S] cluster.

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: Differences in various plasmalogen synthase enzymes based on AlphaFold3 three-dimensional structure predictions. ( A ) E. faecalis K-4, C. perfringens NH13, ( B ) B. suis DSM20211, and ( C ) L. cremoris ATCC BAA-493. Act1 and Act2, activation domains; Red1 and Red2, reduction/dehydration domains. ( D ) [4Fe-4S] cluster.

Article Snippet: In contrast, the corresponding plasmalogen biosynthesis genes in E. faecalis K-4, L. cremoris ATCC BAA-493, and B. longum subsp. suis DSM 20211 were all encoded by a single gene.

Techniques: Activation Assay

HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Article Snippet: However, since PlsLc produced a large amount of plasmalogen, even under aerobic conditions, PlsA from L. cremoris ATCC BAA-493 may have prevented the oxidation of [4Fe-4S] clusters.

Techniques:

Differences in various plasmalogen synthase enzymes based on AlphaFold3 three-dimensional structure predictions. ( A ) E. faecalis K-4, C. perfringens NH13, ( B ) B. suis DSM20211, and ( C ) L. cremoris ATCC BAA-493. Act1 and Act2, activation domains; Red1 and Red2, reduction/dehydration domains. ( D ) [4Fe-4S] cluster.

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: Differences in various plasmalogen synthase enzymes based on AlphaFold3 three-dimensional structure predictions. ( A ) E. faecalis K-4, C. perfringens NH13, ( B ) B. suis DSM20211, and ( C ) L. cremoris ATCC BAA-493. Act1 and Act2, activation domains; Red1 and Red2, reduction/dehydration domains. ( D ) [4Fe-4S] cluster.

Article Snippet: However, since PlsLc produced a large amount of plasmalogen, even under aerobic conditions, PlsA from L. cremoris ATCC BAA-493 may have prevented the oxidation of [4Fe-4S] clusters.

Techniques: Activation Assay

HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Article Snippet: Lactococcus cremoris subsp. cremoris ATCC 14365 , , , , + , NA , ATCC.

Techniques:

HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: HPLC chromatogram of aldehydes from bacterial cell phospholipids treated with 2,4-dinitrophenylhydrazine-HCl. ( A ) Aldehydes detected in L. cremoris ATCC 11602, ATCC 11603, ATCC 14365, and ATCC BAA-493. ( B ) Aldehydes detected in E. faecalis K-4, JCM8726 T , Enterococcus sp. NB13, S. equinus JCM7879 T , and S. mutans JCM5705 T .

Article Snippet: Lactococcus cremoris subsp. cremoris ATCC BAA-493 , , , , + , LACR_2543 , ( ) .

Techniques:

Differences in various plasmalogen synthase enzymes based on AlphaFold3 three-dimensional structure predictions. ( A ) E. faecalis K-4, C. perfringens NH13, ( B ) B. suis DSM20211, and ( C ) L. cremoris ATCC BAA-493. Act1 and Act2, activation domains; Red1 and Red2, reduction/dehydration domains. ( D ) [4Fe-4S] cluster.

Journal: Applied and Environmental Microbiology

Article Title: Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant Escherichia coli expressing anaerobic bacterium-derived plasmalogen synthase genes

doi: 10.1128/aem.00940-25

Figure Lengend Snippet: Differences in various plasmalogen synthase enzymes based on AlphaFold3 three-dimensional structure predictions. ( A ) E. faecalis K-4, C. perfringens NH13, ( B ) B. suis DSM20211, and ( C ) L. cremoris ATCC BAA-493. Act1 and Act2, activation domains; Red1 and Red2, reduction/dehydration domains. ( D ) [4Fe-4S] cluster.

Article Snippet: Lactococcus cremoris subsp. cremoris ATCC BAA-493 , , , , + , LACR_2543 , ( ) .

Techniques: Activation Assay