cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    cre recombinase - by Bioz Stars, 2023-03
    94/100 stars

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    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    High‐throughput screening of polymer library for mRNA delivery. a) NPs were generated after complexation of mRNA with polymers constituted by a fixed diacrylate moiety (P1) with varying bisacrylamides (A–E) and amines (1–32). Polymer library was screened for the delivery of mRNA encoding Cre <t>recombinase</t> in mouse embryonic fibroblast reporter cell line, expressed by functional activation of GFP at 48 h post‐transfection. b) Selected polymers (highlighted in blue after exclusion of false positives) showed comparable transfection efficiency to Lipofectamine 2000. Non‐cytotoxic polymers inducing less Cre‐mediated recombination than naked Cre mRNA were excluded from this analysis. Data are expressed as mean ± SEM (n = 3–14). c) Chemical structures of common bisacrylamides and amines in identified hits. d) Transfection efficiency of NPs containing mRNA Cre recombinase after polymer purification by dialysis. (d.1) Cre‐mediated recombination was quantified by high‐content imaging. Data are expressed as mean ± SEM (n = 6–17). (d.2) Representative fluorescence microscope images of lead NP candidate (P1E28) compared to Lipofectamine 2000. Scale bars = 100 µm. Data in (b) and (d.1) were analyzed by one‐way ANOVA with post hoc Dunnett's multiple comparisons test against free mRNA and untreated controls, respectively: (*), p < 0.05; (**), p < 0.01; (***), p < 0.001; (****), p < 0.0001.
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    cre recombinase - by Bioz Stars, 2023-03
    86/100 stars

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    1) Product Images from "A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts"

    Article Title: A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts

    Journal: Advanced Science

    doi: 10.1002/advs.202205475

    High‐throughput screening of polymer library for mRNA delivery. a) NPs were generated after complexation of mRNA with polymers constituted by a fixed diacrylate moiety (P1) with varying bisacrylamides (A–E) and amines (1–32). Polymer library was screened for the delivery of mRNA encoding Cre recombinase in mouse embryonic fibroblast reporter cell line, expressed by functional activation of GFP at 48 h post‐transfection. b) Selected polymers (highlighted in blue after exclusion of false positives) showed comparable transfection efficiency to Lipofectamine 2000. Non‐cytotoxic polymers inducing less Cre‐mediated recombination than naked Cre mRNA were excluded from this analysis. Data are expressed as mean ± SEM (n = 3–14). c) Chemical structures of common bisacrylamides and amines in identified hits. d) Transfection efficiency of NPs containing mRNA Cre recombinase after polymer purification by dialysis. (d.1) Cre‐mediated recombination was quantified by high‐content imaging. Data are expressed as mean ± SEM (n = 6–17). (d.2) Representative fluorescence microscope images of lead NP candidate (P1E28) compared to Lipofectamine 2000. Scale bars = 100 µm. Data in (b) and (d.1) were analyzed by one‐way ANOVA with post hoc Dunnett's multiple comparisons test against free mRNA and untreated controls, respectively: (*), p < 0.05; (**), p < 0.01; (***), p < 0.001; (****), p < 0.0001.
    Figure Legend Snippet: High‐throughput screening of polymer library for mRNA delivery. a) NPs were generated after complexation of mRNA with polymers constituted by a fixed diacrylate moiety (P1) with varying bisacrylamides (A–E) and amines (1–32). Polymer library was screened for the delivery of mRNA encoding Cre recombinase in mouse embryonic fibroblast reporter cell line, expressed by functional activation of GFP at 48 h post‐transfection. b) Selected polymers (highlighted in blue after exclusion of false positives) showed comparable transfection efficiency to Lipofectamine 2000. Non‐cytotoxic polymers inducing less Cre‐mediated recombination than naked Cre mRNA were excluded from this analysis. Data are expressed as mean ± SEM (n = 3–14). c) Chemical structures of common bisacrylamides and amines in identified hits. d) Transfection efficiency of NPs containing mRNA Cre recombinase after polymer purification by dialysis. (d.1) Cre‐mediated recombination was quantified by high‐content imaging. Data are expressed as mean ± SEM (n = 6–17). (d.2) Representative fluorescence microscope images of lead NP candidate (P1E28) compared to Lipofectamine 2000. Scale bars = 100 µm. Data in (b) and (d.1) were analyzed by one‐way ANOVA with post hoc Dunnett's multiple comparisons test against free mRNA and untreated controls, respectively: (*), p < 0.05; (**), p < 0.01; (***), p < 0.001; (****), p < 0.0001.

    Techniques Used: High Throughput Screening Assay, Generated, Functional Assay, Activation Assay, Transfection, Purification, Imaging, Fluorescence, Microscopy

    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cre recombinase - by Bioz Stars, 2023-03
    94/100 stars

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    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    ( A ) Schematic illustration of the targeting strategy. The cpeb4 gene consists of 10 exons (numbered boxes) and spans a region of approximately 60-kb (WT, wild-type). The targeting vector containing the flip <t>recombinase</t> target (FRT)-flanked neomycin-resistance gene (Neo) and loxP-flanked exon 2 cassette was inserted into the cpeb4 gene by homologous recombination. The correctly targeted locus was examined by Southern blotting using DNA digested with BamHI (B1) or BglII (B2) and probed with a 5′-end or 3′-end probe, respectively. The mouse line carrying the floxed allele of CPEB4 (fCPEB4) was then crossed with a transgenic male mouse expressing Cre recombinase in the sperm (protamine-Cre) to produce the knockout (KO) allele. ( B ) The WT and KO alleles were distinguished on a Southern blot using DNA digested with HindIII (H3). The brain RNAs isolated from male offspring carrying WT (+/+), heterozygous (+/−) or KO (−/−) alleles were analyzed by ( C ) Northern blotting using a probe against exon 1 of CPEB4 RNA or GAPDH RNA (loading control). ( D ) RT-qPCR analysis. The brain RNAs were reverse transcribed and PCR-amplified for CPEB4 and β-actin. The CPEB4 RNA level after normalization with the β-actin signal was expressed as a relative ratio to the WT which was arbitrarily set to 100%. ( E ) RT-PCR confirmed the presence of exon 2-deleted CPEB4 transcripts in the KO brain. The doublet bands amplified from the WT or KO cDNA were resulted from the presence of two alternatively spliced isoforms (4a and 4b). PTC, premature termination codon.
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cre recombinase - by Bioz Stars, 2023-03
    94/100 stars

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    1) Product Images from "CPEB4 Knockout Mice Exhibit Normal Hippocampus-Related Synaptic Plasticity and Memory"

    Article Title: CPEB4 Knockout Mice Exhibit Normal Hippocampus-Related Synaptic Plasticity and Memory

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0084978

    ( A ) Schematic illustration of the targeting strategy. The cpeb4 gene consists of 10 exons (numbered boxes) and spans a region of approximately 60-kb (WT, wild-type). The targeting vector containing the flip recombinase target (FRT)-flanked neomycin-resistance gene (Neo) and loxP-flanked exon 2 cassette was inserted into the cpeb4 gene by homologous recombination. The correctly targeted locus was examined by Southern blotting using DNA digested with BamHI (B1) or BglII (B2) and probed with a 5′-end or 3′-end probe, respectively. The mouse line carrying the floxed allele of CPEB4 (fCPEB4) was then crossed with a transgenic male mouse expressing Cre recombinase in the sperm (protamine-Cre) to produce the knockout (KO) allele. ( B ) The WT and KO alleles were distinguished on a Southern blot using DNA digested with HindIII (H3). The brain RNAs isolated from male offspring carrying WT (+/+), heterozygous (+/−) or KO (−/−) alleles were analyzed by ( C ) Northern blotting using a probe against exon 1 of CPEB4 RNA or GAPDH RNA (loading control). ( D ) RT-qPCR analysis. The brain RNAs were reverse transcribed and PCR-amplified for CPEB4 and β-actin. The CPEB4 RNA level after normalization with the β-actin signal was expressed as a relative ratio to the WT which was arbitrarily set to 100%. ( E ) RT-PCR confirmed the presence of exon 2-deleted CPEB4 transcripts in the KO brain. The doublet bands amplified from the WT or KO cDNA were resulted from the presence of two alternatively spliced isoforms (4a and 4b). PTC, premature termination codon.
    Figure Legend Snippet: ( A ) Schematic illustration of the targeting strategy. The cpeb4 gene consists of 10 exons (numbered boxes) and spans a region of approximately 60-kb (WT, wild-type). The targeting vector containing the flip recombinase target (FRT)-flanked neomycin-resistance gene (Neo) and loxP-flanked exon 2 cassette was inserted into the cpeb4 gene by homologous recombination. The correctly targeted locus was examined by Southern blotting using DNA digested with BamHI (B1) or BglII (B2) and probed with a 5′-end or 3′-end probe, respectively. The mouse line carrying the floxed allele of CPEB4 (fCPEB4) was then crossed with a transgenic male mouse expressing Cre recombinase in the sperm (protamine-Cre) to produce the knockout (KO) allele. ( B ) The WT and KO alleles were distinguished on a Southern blot using DNA digested with HindIII (H3). The brain RNAs isolated from male offspring carrying WT (+/+), heterozygous (+/−) or KO (−/−) alleles were analyzed by ( C ) Northern blotting using a probe against exon 1 of CPEB4 RNA or GAPDH RNA (loading control). ( D ) RT-qPCR analysis. The brain RNAs were reverse transcribed and PCR-amplified for CPEB4 and β-actin. The CPEB4 RNA level after normalization with the β-actin signal was expressed as a relative ratio to the WT which was arbitrarily set to 100%. ( E ) RT-PCR confirmed the presence of exon 2-deleted CPEB4 transcripts in the KO brain. The doublet bands amplified from the WT or KO cDNA were resulted from the presence of two alternatively spliced isoforms (4a and 4b). PTC, premature termination codon.

    Techniques Used: Plasmid Preparation, Homologous Recombination, Southern Blot, Transgenic Assay, Expressing, Knock-Out, Isolation, Northern Blot, Quantitative RT-PCR, Amplification, Reverse Transcription Polymerase Chain Reaction

    recombinant cre recombinase  (New England Biolabs)


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    New England Biolabs recombinant cre recombinase
    Recombinant Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    cre recombinase - by Bioz Stars, 2023-03
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    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    cre lox recombination  (New England Biolabs)


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    New England Biolabs cre lox recombination
    Cre Lox Recombination, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    cre recombinase  (New England Biolabs)


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    New England Biolabs cre recombinase
    Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    New England Biolabs cre recombinase
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    https://www.bioz.com/result/cre recombinase/product/New England Biolabs
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    recombinant cre recombinase  (New England Biolabs)
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    New England Biolabs recombinant cre recombinase
    Recombinant Cre Recombinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant cre recombinase/product/New England Biolabs
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