cpg dna fragments (Millipore)
93
Structured Review
Millipore
cpg dna fragments
Cpg Dna Fragments, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg dna fragments/product/Millipore
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Cpg Dna Fragments, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cpg dna fragments/product/Millipore
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cpg dna fragments - by Bioz Stars,
2021-04
93/100 stars
Images
1) Product Images from "DNA methylation prevents CTCF-mediated silencing of the oncogene BCL6 in B cell lymphomas"
Article Title: DNA methylation prevents CTCF-mediated silencing of the oncogene BCL6 in B cell lymphomas
Journal: The Journal of Experimental Medicine
doi: 10.1084/jem.20100204
Figure Legend Snippet: Presence of DNase I hypersensitive sites within CpG islands 32 and 27 in the absence of DNA methylation. (A) The diagram depicts the approximate location of the genomic features of the BCL6 locus along with the restriction fragments and probes used for indirect end labeling. The approximate locations of the observed hypersensitive sites are indicated on the diagram. (B) The 5′ end of the BCL6 locus was analyzed by DNase I digestion using the reference restriction enzyme XbaI. Wedges indicate concentrations of DNase I. The locations of restriction sites and the probe used for the Southern blot are depicted in A. Arrows indicate the major DNase I hypersensitive sites and their locations are summarized in A. Marker positions were measured from ethidium-stained gel before transfer. Data shown is representative of results from at least three independent experiments.
Techniques Used: DNA Methylation Assay, End Labeling, Southern Blot, Marker, Staining
Figure Legend Snippet: Cell type–specific DNA methylation status at BCL6 intronic region. (A) The diagram depicts the genomic organization of the human BCL6 locus. Indicated are the two transcription start sites (arrows), the four CpG islands (17, 32, 27, and 38), and the exons (black rectangles). Below the cartoon, the results of genomic bisulfite sequencing are presented. Each line of circles indicates an individual clone sequenced in the analysis after bisulfite treatment and PCR. Open circles indicate CpG sites at which no DNA methylation is detected. Blackened circles indicate CpG sites which are methylated. Data shown is representative of the results of three independent biological replicates. (B) MIRA-chip analysis of Raji (top) and H929 (bottom) across the 5′ end of the BCL6 locus. Each vertical line represents the mean normalized log 2 ratio of enriched/input probe signal from two replicate samples, corresponding to the genomic location on chromosome 3 at the BCL6 locus (UCSC genome browser HG18) as listed on the x axis. Blue and yellow colors represent methylated and unmethylated regions, respectively.
Techniques Used: DNA Methylation Assay, Methylation Sequencing, Polymerase Chain Reaction, Methylation, Chromatin Immunoprecipitation
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