Journal: International Journal of Endocrinology

Article Title: Gomisin N Improves Insulin Secretion by Alleviating Endoplasmic Reticulum and Oxidative Stress in Pancreatic Beta Cells

doi: 10.1155/ije/9046981

Figure Lengend Snippet: Effects of gomisin N (Go) on endoplasmic reticulum (ER) stress markers in pancreatic β ‐cells. Mouse pancreatic β ‐cell lines were treated with 50 μM CPA alone or cotreated with 50 μM CPA and 25 μM Go for 24 h. The mRNA expression levels of representative ER stress–related genes were quantified by real‐time quantitative PCR (A–F). Gene expression was normalized to the housekeeping gene Rplp0 to minimize interexperiment variability. The sample size was six ( n = 6). Statistical significance was evaluated relative to the untreated control group, and p values are indicated.

Article Snippet: CPA (catalog no. HY‐N6686; MedChemExpress, Monmouth Junction, NJ, USA) and gomisin N (catalog no. CFN90125 ; ChemFaces, Wuhan, China) were used in this study.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Gene Expression, Control

Journal: International Journal of Endocrinology

Article Title: Gomisin N Improves Insulin Secretion by Alleviating Endoplasmic Reticulum and Oxidative Stress in Pancreatic Beta Cells

doi: 10.1155/ije/9046981

Figure Lengend Snippet: Gomisin N (Go) attenuates CPA‐induced ER stress responses and improves insulin secretion in pancreatic β ‐cells. Mouse pancreatic β ‐cell lines were treated with 50 μM CPA alone or cotreated with 50 μM CPA and 25 μM Go for 24 h. (A) Representative western blot images showing protein expression of the ER stress markers GRP94, BiP/GRP78, and ATF4. Actin served as a loading control. (B–D) Band intensities were quantified from three independent experiments using ImageJ, and the data are presented as scatter plots with mean ± SEM. (E) To evaluate β ‐cell function, cells were first incubated in Krebs–Ringer buffer containing 2.8 mM glucose to determine basal insulin secretion, followed by stimulation with Krebs–Ringer buffer containing 16.8 mM glucose to induce insulin release. Secreted insulin in the culture medium was measured using a high‐sensitivity insulin ELISA kit. Data are presented as mean ± SEM from three independent experiments. Statistical significance was assessed, and p values are reported.

Article Snippet: CPA (catalog no. HY‐N6686; MedChemExpress, Monmouth Junction, NJ, USA) and gomisin N (catalog no. CFN90125 ; ChemFaces, Wuhan, China) were used in this study.

Techniques: Western Blot, Expressing, Control, Cell Function Assay, Incubation, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Clinical-grade cryopreservation unlocks transplant-ready human pancreatic and stem cell–derived islets for diabetes therapy

doi: 10.64898/2026.04.25.720819

Figure Lengend Snippet: ( A ) Human pancreatic islets were isolated from donor pancreases by enzymatic distension of the head and tail, digestion in a Ricordi chamber with agitation, and density-gradient purification . ( B ) Human embryonic stem cells were expanded and differentiated into stem cell–derived β cell islets (SC-islets) using a six-stage growth factor protocol . ( C ) Vitrification on gold-coated copper (Cu-Au) CryoMesh involved stepwise CPA loading, transfer of islets or SC-islets onto the mesh, removal of excess CPA by wicking, rapid plunging into liquid nitrogen, long-term cryobanking, and stepwise CPA unloading after rewarming. ( D ) Potential clinical benefits of islet cryopreservation include increased donor organ utilization, cryobanking of large islet or SC-islets batches as single-patient doses, improved donor–recipient matching, pooled donor transplantation, flexible scheduling of procedures, more time for batch quality assessment and safety testing, opportunities for immune tolerance induction, and global distribution. CPA, cryoprotective agent; hESC, human embryonic stem cell; LN 2 , liquid nitrogen

Article Snippet: CPA Loading Solutions: Solution 1, 4.4 wt% ethylene glycol (EG, Fisher Scientific), 4.4 wt% dimethyl sulfoxide (DMSO, Fisher Scientific); Solution 2, 11 wt% EG, 11 wt% DMSO; and Solution 3: 22 wt% EG, 22 wt% DMSO.

Techniques: Isolation, Purification, Derivative Assay, Transplantation Assay