cp 66713  (Santa Cruz Biotechnology)

Journal: Frontiers in Physiology

Article Title: Adenosine stimulates the basolateral 50 pS K + channel in renal proximal tubule via adenosine-A1 receptor

doi: 10.3389/fphys.2023.1242975

Figure Lengend Snippet: Inhibition of adenosine-A2 receptor fails to abolish the effect of adenosine on the basolateral 50 pS K + channel of the PT. (A) A single channel recording (in a cell-attached patch) shows the time course of adenosine effect (1 μm) on the 50 pS K + channel in the presence of 10 μm CP-66713, an adenosine A 2 receptor antagonist. The experiments were performed in the PT pretreated with CP-66713 (which was continuously present in the bath). Two parts of the trace indicated by numbers are extended to show the fast time resolution. Holding potential is at 0 mV “C” indicates that the channel close level. (B) A Bar graph summarizes the effect of adenosine on the 50 pS K + channel in the presence of CP-66713.

Article Snippet: Collagenase II, DPCPX, CP-66713, AACOCF3, Calphostin C, U73122, H-8 (N—[2- (methylamino) ethyl] -5- isoquinoline sulfonamide-2HC1), and the chemical reagent used for preparation of above solution were all purchased from Sigma.

Techniques: Inhibition

Journal: Molecular pharmacology

Article Title: A Binding Site Model and Structure-Activity Relationships for the Rat A 3 Adenosine Receptor

doi:

Figure Lengend Snippet: Affinities of selected compounds at A 1 A 2a and A 3 receptors, indicated as either K i , (nM) or percent displacement at a concentration of 100 μ M, unless otherwise indicated Values expressed as means ± S.E. were all measured in this study (three to five experiments). K i , values at A 1 and A 2a receptors provided without S.E. are taken from the literature as indicated.

Article Snippet: The following compounds were gifts, which are gratefully acknowledged: 1,3-dibutylxanthine, 1,3-dihexylxanthine, 1,3-dibenzylxanthine, 8-cyclohexycaffeine, 7-benzyltheophylline, N 6 -dimethyladenosine, 3-deazaadenosine, 7-deazaadenosine, β -L-adenosine, 2′- 0 -methyladenosine, adenine- β -D-arabinofuranoside, xylofuranosyladenosine, β -D-psicofuranosyladenine, 5′-deoxy-5′-aminoadenosine, 5′-carboxamidoadenosine, 2-thio-3-propylxanthine, 1-propyl-8-cyclopentylxanthine (Dr. J. Daly, NIH, Bethesda, MD); N 6 -cyclohexylNECA, 9-ethyl- N 6 -cyclopentyladenine, N 6 -dimethylNECA, N 6 -benzyl- N 6 -methyladenosine (Dr. R. Olsson, University of South Florida, Tampa, FL); CP 66713 (Dr. R. Sarges, Pfizer, Groton, CT); CGS 15943, (Dr. J. Francis, Ciba-Geigy, Summit, NJ).

Techniques: Concentration Assay