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fluidigm count mrna molecules
Sensitivity of detecting numbers of DNA and RNA molecules. Real-time <t>PCR</t> was performed over a concentration range from A) TNF DNA standard template or B) TNF <t>mRNA</t> standard template. cDNA synthesis was performed on the dilution series of mRNA samples and the difference in signal between the same amount of input mRNA (▪) versus input DNA (ο) indicates a reverse transcription efficiency of 54% in this experiment. For each copy number, the mean and standard deviation are shown for 12 samples in A) and 4 samples in B). The Y-axis is a log 10 rescaling of the C t values.
Count Mrna Molecules, supplied by fluidigm, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/count mrna molecules/product/fluidigm
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1) Product Images from "Resolving Cell Population Heterogeneity: Real-Time PCR for Simultaneous Multiplexed Gene Detection in Multiple Single-Cell Samples"

Article Title: Resolving Cell Population Heterogeneity: Real-Time PCR for Simultaneous Multiplexed Gene Detection in Multiple Single-Cell Samples

Journal: PLoS ONE

doi: 10.1371/journal.pone.0006326

Sensitivity of detecting numbers of DNA and RNA molecules. Real-time PCR was performed over a concentration range from A) TNF DNA standard template or B) TNF mRNA standard template. cDNA synthesis was performed on the dilution series of mRNA samples and the difference in signal between the same amount of input mRNA (▪) versus input DNA (ο) indicates a reverse transcription efficiency of 54% in this experiment. For each copy number, the mean and standard deviation are shown for 12 samples in A) and 4 samples in B). The Y-axis is a log 10 rescaling of the C t values.
Figure Legend Snippet: Sensitivity of detecting numbers of DNA and RNA molecules. Real-time PCR was performed over a concentration range from A) TNF DNA standard template or B) TNF mRNA standard template. cDNA synthesis was performed on the dilution series of mRNA samples and the difference in signal between the same amount of input mRNA (▪) versus input DNA (ο) indicates a reverse transcription efficiency of 54% in this experiment. For each copy number, the mean and standard deviation are shown for 12 samples in A) and 4 samples in B). The Y-axis is a log 10 rescaling of the C t values.

Techniques Used: Real-time Polymerase Chain Reaction, Concentration Assay, Standard Deviation

Sensitivity for single-cell mRNA measurements. Macrophages were activated with the bacterial stimulus, lipopolysaccharide (30 ng/ml for 2 hours), and the indicated number of cells (1, 10 or 100 cells) were sorted by flow cytometry. mRNA expression of the indicated genes was measured by real-time PCR using 1/8 th of the sample cDNA lysate per measurement. The mean and standard deviation of 12 samples are presented for each of the indicated number of cells. The C t values were arbitrarily scaled to log 10 values (y-axis).
Figure Legend Snippet: Sensitivity for single-cell mRNA measurements. Macrophages were activated with the bacterial stimulus, lipopolysaccharide (30 ng/ml for 2 hours), and the indicated number of cells (1, 10 or 100 cells) were sorted by flow cytometry. mRNA expression of the indicated genes was measured by real-time PCR using 1/8 th of the sample cDNA lysate per measurement. The mean and standard deviation of 12 samples are presented for each of the indicated number of cells. The C t values were arbitrarily scaled to log 10 values (y-axis).

Techniques Used: Flow Cytometry, Cytometry, Expressing, Real-time Polymerase Chain Reaction, Standard Deviation

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Real-time Polymerase Chain Reaction:

Article Title: Resolving Cell Population Heterogeneity: Real-Time PCR for Simultaneous Multiplexed Gene Detection in Multiple Single-Cell Samples
Article Snippet: .. Single samples also can be processed for real-time PCR on the Fluidigm instrument to count mRNA molecules , . ..

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    fluidigm count mrna molecules
    Sensitivity of detecting numbers of DNA and RNA molecules. Real-time <t>PCR</t> was performed over a concentration range from A) TNF DNA standard template or B) TNF <t>mRNA</t> standard template. cDNA synthesis was performed on the dilution series of mRNA samples and the difference in signal between the same amount of input mRNA (▪) versus input DNA (ο) indicates a reverse transcription efficiency of 54% in this experiment. For each copy number, the mean and standard deviation are shown for 12 samples in A) and 4 samples in B). The Y-axis is a log 10 rescaling of the C t values.
    Count Mrna Molecules, supplied by fluidigm, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/count mrna molecules/product/fluidigm
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    count mrna molecules - by Bioz Stars, 2020-09
    85/100 stars
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    Sensitivity of detecting numbers of DNA and RNA molecules. Real-time PCR was performed over a concentration range from A) TNF DNA standard template or B) TNF mRNA standard template. cDNA synthesis was performed on the dilution series of mRNA samples and the difference in signal between the same amount of input mRNA (▪) versus input DNA (ο) indicates a reverse transcription efficiency of 54% in this experiment. For each copy number, the mean and standard deviation are shown for 12 samples in A) and 4 samples in B). The Y-axis is a log 10 rescaling of the C t values.

    Journal: PLoS ONE

    Article Title: Resolving Cell Population Heterogeneity: Real-Time PCR for Simultaneous Multiplexed Gene Detection in Multiple Single-Cell Samples

    doi: 10.1371/journal.pone.0006326

    Figure Lengend Snippet: Sensitivity of detecting numbers of DNA and RNA molecules. Real-time PCR was performed over a concentration range from A) TNF DNA standard template or B) TNF mRNA standard template. cDNA synthesis was performed on the dilution series of mRNA samples and the difference in signal between the same amount of input mRNA (▪) versus input DNA (ο) indicates a reverse transcription efficiency of 54% in this experiment. For each copy number, the mean and standard deviation are shown for 12 samples in A) and 4 samples in B). The Y-axis is a log 10 rescaling of the C t values.

    Article Snippet: Single samples also can be processed for real-time PCR on the Fluidigm instrument to count mRNA molecules , .

    Techniques: Real-time Polymerase Chain Reaction, Concentration Assay, Standard Deviation

    Sensitivity for single-cell mRNA measurements. Macrophages were activated with the bacterial stimulus, lipopolysaccharide (30 ng/ml for 2 hours), and the indicated number of cells (1, 10 or 100 cells) were sorted by flow cytometry. mRNA expression of the indicated genes was measured by real-time PCR using 1/8 th of the sample cDNA lysate per measurement. The mean and standard deviation of 12 samples are presented for each of the indicated number of cells. The C t values were arbitrarily scaled to log 10 values (y-axis).

    Journal: PLoS ONE

    Article Title: Resolving Cell Population Heterogeneity: Real-Time PCR for Simultaneous Multiplexed Gene Detection in Multiple Single-Cell Samples

    doi: 10.1371/journal.pone.0006326

    Figure Lengend Snippet: Sensitivity for single-cell mRNA measurements. Macrophages were activated with the bacterial stimulus, lipopolysaccharide (30 ng/ml for 2 hours), and the indicated number of cells (1, 10 or 100 cells) were sorted by flow cytometry. mRNA expression of the indicated genes was measured by real-time PCR using 1/8 th of the sample cDNA lysate per measurement. The mean and standard deviation of 12 samples are presented for each of the indicated number of cells. The C t values were arbitrarily scaled to log 10 values (y-axis).

    Article Snippet: Single samples also can be processed for real-time PCR on the Fluidigm instrument to count mRNA molecules , .

    Techniques: Flow Cytometry, Cytometry, Expressing, Real-time Polymerase Chain Reaction, Standard Deviation