Structured Review

Roche cot 1 human dna
Cot 1 Human Dna, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cot 1 human dna/product/Roche
Average 89 stars, based on 12 article reviews
Price from $9.99 to $1999.99
cot 1 human dna - by Bioz Stars, 2020-07
89/100 stars

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Related Articles

Labeling:

Article Title: Cellular, Molecular and Functional Characterisation of YAC Transgenic Mouse Models of Friedreich Ataxia
Article Snippet: .. The labeled DNAs were ethanol precipitated together with Cot-1 human DNA (Roche) and resuspended in 10 µl of hybridisation buffer (Sigma). .. Hybridisation was at 37°C overnight followed by washing with 2× SSC for 5 minutes.

Hybridization:

Article Title: Cellular, Molecular and Functional Characterisation of YAC Transgenic Mouse Models of Friedreich Ataxia
Article Snippet: .. The labeled DNAs were ethanol precipitated together with Cot-1 human DNA (Roche) and resuspended in 10 µl of hybridisation buffer (Sigma). .. Hybridisation was at 37°C overnight followed by washing with 2× SSC for 5 minutes.

Article Title: Molecular Characterization of the Region 7q22.1 in Splenic Marginal Zone Lymphomas
Article Snippet: .. Labelled test and reference DNA samples were mixed equitably, co-precipitated in the presence of Cot-1 human DNA (Roche, Indianapolis, IN) with ethanol, washed, and resuspended in hybridization solution (50% formamide, 10% Dextran sulphate, 2× standard saline citrate, 10 mM Tris pH 7.6, 2.7% sodium dodecyl sulphate and 10 µg/µl of yeast tRNA). ..

Article Title: A novel GAA-repeat-expansion-based mouse model of Friedreich’s ataxia
Article Snippet: .. The labelled DNAs were ethanol precipitated together with Cot-1 human DNA (Roche) and resuspended in 10 μl of hybridisation buffer (Sigma). .. Hybridisation was carried out at 37°C overnight followed by washing with 2× SSC for 5 minutes.

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  • 92
    Roche digoxigenin labeled
    Construction of iHAC vectors. (A) A map of the reprogramming cassette constructed in a PAC vector, pPH3-9. The expression cassette comprised cDNAs for Klf4, c-Myc, Sox2 and Oct4, each under the control of a CAG promoter and flanked with HS4 insulators. The reprogramming cassette was followed by exon 3 to exon 9 of the human HPRT gene and then by a lox P site. (B) Schematic maps of the iHAC1 and iHAC2 inserts. iHAC1 has a duplicated reprogramming cassette. iHAC2 has this duplicated cassette and an additional cassette with two copies of Oct4 and a p53 shRNA construct. (C) FISH analyses of CHO/iHAC1 and CHO/iHAC2. <t>Digoxigenin-labeled</t> human COT-1 DNA (red) was used to detect the HAC backbone. Biotin-labeled pPAC-KMSO (green) was used to detect the reprogramming cassette in the iHACs. Chromosomal DNA was counterstained with DAPI. White arrows indicate iHAC vectors and the insets show enlarged images of the iHACs.
    Digoxigenin Labeled, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/digoxigenin labeled/product/Roche
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    digoxigenin labeled - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    86
    Roche digoxigenin labeled dna probe human cot 1 dna
    A novel assay with <t>Cot-1</t> (repeat) <t>DNA</t> as a probe broadly detects nucleoplasmic hnRNA and discriminates regions of more or less active chromatin
    Digoxigenin Labeled Dna Probe Human Cot 1 Dna, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/digoxigenin labeled dna probe human cot 1 dna/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    digoxigenin labeled dna probe human cot 1 dna - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    91
    Roche mouse cot 1 dna
    <t>CoT-1</t> RNA appears highly abundant A) XIST RNA paints interphase X-chromosome territory. Scale bar 5µm. B) The DAPI dense Barr Body (arrow) defines the inactive X-chromosome painted by XIST RNA. C) Both CoT-1 and XIST RNAs are detected simultaneously with the same fluorochrome. D) Linescan (white line in C) through the Barr body (arrow) illustrates similar intensities for both RNAs (green). E–H) CoT-1 RNA is more abundant than rRNA. All images are the same exposure. E) CoT-1 RNA alone, F) nucleolar 18S rRNA alone (Insert is same cell with DAPI <t>DNA</t> included), G) CoT-1 RNA and 18S rRNA detected with same fluorochrome. H) Average total intensities were measured for each RNA signal (n=40).
    Mouse Cot 1 Dna, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse cot 1 dna/product/Roche
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mouse cot 1 dna - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    89
    Roche cot 1 human dna
    <t>CoT-1</t> RNA appears highly abundant A) XIST RNA paints interphase X-chromosome territory. Scale bar 5µm. B) The DAPI dense Barr Body (arrow) defines the inactive X-chromosome painted by XIST RNA. C) Both CoT-1 and XIST RNAs are detected simultaneously with the same fluorochrome. D) Linescan (white line in C) through the Barr body (arrow) illustrates similar intensities for both RNAs (green). E–H) CoT-1 RNA is more abundant than rRNA. All images are the same exposure. E) CoT-1 RNA alone, F) nucleolar 18S rRNA alone (Insert is same cell with DAPI <t>DNA</t> included), G) CoT-1 RNA and 18S rRNA detected with same fluorochrome. H) Average total intensities were measured for each RNA signal (n=40).
    Cot 1 Human Dna, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cot 1 human dna/product/Roche
    Average 89 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    cot 1 human dna - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    Image Search Results


    Construction of iHAC vectors. (A) A map of the reprogramming cassette constructed in a PAC vector, pPH3-9. The expression cassette comprised cDNAs for Klf4, c-Myc, Sox2 and Oct4, each under the control of a CAG promoter and flanked with HS4 insulators. The reprogramming cassette was followed by exon 3 to exon 9 of the human HPRT gene and then by a lox P site. (B) Schematic maps of the iHAC1 and iHAC2 inserts. iHAC1 has a duplicated reprogramming cassette. iHAC2 has this duplicated cassette and an additional cassette with two copies of Oct4 and a p53 shRNA construct. (C) FISH analyses of CHO/iHAC1 and CHO/iHAC2. Digoxigenin-labeled human COT-1 DNA (red) was used to detect the HAC backbone. Biotin-labeled pPAC-KMSO (green) was used to detect the reprogramming cassette in the iHACs. Chromosomal DNA was counterstained with DAPI. White arrows indicate iHAC vectors and the insets show enlarged images of the iHACs.

    Journal: PLoS ONE

    Article Title: Integration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors

    doi: 10.1371/journal.pone.0025961

    Figure Lengend Snippet: Construction of iHAC vectors. (A) A map of the reprogramming cassette constructed in a PAC vector, pPH3-9. The expression cassette comprised cDNAs for Klf4, c-Myc, Sox2 and Oct4, each under the control of a CAG promoter and flanked with HS4 insulators. The reprogramming cassette was followed by exon 3 to exon 9 of the human HPRT gene and then by a lox P site. (B) Schematic maps of the iHAC1 and iHAC2 inserts. iHAC1 has a duplicated reprogramming cassette. iHAC2 has this duplicated cassette and an additional cassette with two copies of Oct4 and a p53 shRNA construct. (C) FISH analyses of CHO/iHAC1 and CHO/iHAC2. Digoxigenin-labeled human COT-1 DNA (red) was used to detect the HAC backbone. Biotin-labeled pPAC-KMSO (green) was used to detect the reprogramming cassette in the iHACs. Chromosomal DNA was counterstained with DAPI. White arrows indicate iHAC vectors and the insets show enlarged images of the iHACs.

    Article Snippet: FISH analysis FISH analyses were performed on either fixed metaphase or interphase spreads of each cell hybrid using digoxigenin-labeled (Roche) human COT-1 DNA (Invitrogen) and biotin-labeled pPAC-KMSO, essentially as described previously .

    Techniques: Construct, Plasmid Preparation, Expressing, shRNA, Fluorescence In Situ Hybridization, Labeling, HAC Assay

    A novel assay with Cot-1 (repeat) DNA as a probe broadly detects nucleoplasmic hnRNA and discriminates regions of more or less active chromatin

    Journal: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

    Article Title: A Multifaceted FISH Approach to Study Endogenous RNAs and DNAs within Native Nuclear and Cell Structure

    doi: 10.1002/0471142905.hg0415s76

    Figure Lengend Snippet: A novel assay with Cot-1 (repeat) DNA as a probe broadly detects nucleoplasmic hnRNA and discriminates regions of more or less active chromatin

    Article Snippet: 50 ng Biotin or Digoxigenin labeled DNA probe Human Cot-1 DNA (Roche) or Mouse Cot-1 DNA (Invitrogen) Salmon Sperm DNA + E. Coli tRNA 100% ethanol (4°C or colder) Formamide (Sigma) Hybridization buffer RNase inhibitor (RNasin-Promega or RNase OUT-Invitrogen) Parafilm 4× SSC, 2× SSC, and 1× SSC 1% BSA in 4xSSC (w/v) 0.1%Triton X-100 (Roche) in 4xSSC (v/v) Dylight 488 or 594 Streptavidin (Jackson Immuno Research) or Anti-digoxigenin Fluorescein (Roche) DAPI (Sigma) Glass Micro Slides (Corning) Vectashield (Vector Labs) Nail polish (clear) Slide folders

    Techniques:

    CoT-1 RNA appears highly abundant A) XIST RNA paints interphase X-chromosome territory. Scale bar 5µm. B) The DAPI dense Barr Body (arrow) defines the inactive X-chromosome painted by XIST RNA. C) Both CoT-1 and XIST RNAs are detected simultaneously with the same fluorochrome. D) Linescan (white line in C) through the Barr body (arrow) illustrates similar intensities for both RNAs (green). E–H) CoT-1 RNA is more abundant than rRNA. All images are the same exposure. E) CoT-1 RNA alone, F) nucleolar 18S rRNA alone (Insert is same cell with DAPI DNA included), G) CoT-1 RNA and 18S rRNA detected with same fluorochrome. H) Average total intensities were measured for each RNA signal (n=40).

    Journal: Cell

    Article Title: Stable CoT-1 repeat RNA is abundant and associated with euchromatic interphase chromosomes

    doi: 10.1016/j.cell.2014.01.042

    Figure Lengend Snippet: CoT-1 RNA appears highly abundant A) XIST RNA paints interphase X-chromosome territory. Scale bar 5µm. B) The DAPI dense Barr Body (arrow) defines the inactive X-chromosome painted by XIST RNA. C) Both CoT-1 and XIST RNAs are detected simultaneously with the same fluorochrome. D) Linescan (white line in C) through the Barr body (arrow) illustrates similar intensities for both RNAs (green). E–H) CoT-1 RNA is more abundant than rRNA. All images are the same exposure. E) CoT-1 RNA alone, F) nucleolar 18S rRNA alone (Insert is same cell with DAPI DNA included), G) CoT-1 RNA and 18S rRNA detected with same fluorochrome. H) Average total intensities were measured for each RNA signal (n=40).

    Article Snippet: FISH probes : 4kb human L1.3 ORF2, and L1.3 ORF1 (from J. Moran, UMich), PCR generated 3’ and 5’ L1Hs UTR, and 2kb DMPK intron 9 , introns (non-repetitive) for XIST, COL1A1 and GAPDH were PCR generated and cloned into pSC-A (Stratagene) , 10kb human XIST pG1A,10kb 18S rDNA (from G. Stein, UMMS), 330bp Human Alu pPD39 clone (ATTC), human and mouse CoT-1 DNA (Roche and Invitrogen respectively), and two Chr4 paints (Qbiogene & Cytocell).

    Techniques: