controls kit  (Thermo Fisher)


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    Name:
    GeneChip WT Terminal Labeling and Controls Kit
    Description:
    The GeneChip WT Terminal Labeling and Controls Kit should be combined with the WT Expression Kit sold separately as the most effective option for target preparation on GeneChip Exon and Gene 1 0 ST Arrays The basic chemistry is the same as the GeneChip WT Sense Target Labeling and Control Reagents kit however the WT Expression Kit utilizes a streamlined protocol with master mixes does not require an upfront rRNA reduction step for optimal performance and uses optimized reagents that enable lower total RNA input amounts The GeneChip WT Terminal Labeling and Controls Kit contains the GeneChip WT Terminal Labeling Kit GeneChip Poly A Control Kit and GeneChip Hybridization Control Kit Key Features WT Expression Kit 50 ng total RNA input No ribosomal removal required for Human Mouse and Rat Exon 1 0 ST and Gene 1 0 ST Arrays Streamlined workflow for a simplified protocol and reduced hands on time Magnetic bead based purification for enhanced recovery The WT Expression Kit includes All reagents needed to generate sense cDNA ready for fragmentation and labeling Magnetic beads for enhanced purification efficiency Plastic consumables required to run the assay The GeneChip WT Terminal Labeling and Controls Kit includes WT Fragmentation and Labeling Kit required for target produced by the WT Expression Kit Poly A controls that are added to input RNA to measure efficiency of target amplification by the WT Expression Kit Hybridization controls that monitor the hybridization process for troubleshooting purposes What you need to order • For 10 reactions WT Expression Kit Cat No 4411973 10 reactions GeneChip WT Terminal Labeling and Controls Kit Cat No 901525 10 reactions Additional GeneChip WT Terminal Labeling Kit 10 reactions Cat No 900670 is available separately • For 30 reactions WT Expression Kit Cat No 4411974 10 reactions GeneChip WT Terminal Labeling and Controls Kit Cat No 901524 10 reactions Documentation The following documentation is required for using the WT Expression Kit and the GeneChip WT Terminal Labeling and Controls Kit with Exon and Gene 1 0 ST Arrays • Protocol WT Expression Kit Cat No 4425209 • User Manual GeneChip WT Terminal Labeling and Hybridization Cat No 702808 • User Manual GeneChip Expression Wash Stain and Scan Cat No 702731 Related LinksGeneChip Hybridization Wash and Stain KitGeneChip WT Terminal Labeling and Controls KitatSNPtilx520433 Arabidopsis Cartridge Array
    Catalog Number:
    901524
    Price:
    None
    Applications:
    DNA & RNA Microarray Analysis|Microarray Analysis
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher controls kit
    The GeneChip WT Terminal Labeling and Controls Kit should be combined with the WT Expression Kit sold separately as the most effective option for target preparation on GeneChip Exon and Gene 1 0 ST Arrays The basic chemistry is the same as the GeneChip WT Sense Target Labeling and Control Reagents kit however the WT Expression Kit utilizes a streamlined protocol with master mixes does not require an upfront rRNA reduction step for optimal performance and uses optimized reagents that enable lower total RNA input amounts The GeneChip WT Terminal Labeling and Controls Kit contains the GeneChip WT Terminal Labeling Kit GeneChip Poly A Control Kit and GeneChip Hybridization Control Kit Key Features WT Expression Kit 50 ng total RNA input No ribosomal removal required for Human Mouse and Rat Exon 1 0 ST and Gene 1 0 ST Arrays Streamlined workflow for a simplified protocol and reduced hands on time Magnetic bead based purification for enhanced recovery The WT Expression Kit includes All reagents needed to generate sense cDNA ready for fragmentation and labeling Magnetic beads for enhanced purification efficiency Plastic consumables required to run the assay The GeneChip WT Terminal Labeling and Controls Kit includes WT Fragmentation and Labeling Kit required for target produced by the WT Expression Kit Poly A controls that are added to input RNA to measure efficiency of target amplification by the WT Expression Kit Hybridization controls that monitor the hybridization process for troubleshooting purposes What you need to order • For 10 reactions WT Expression Kit Cat No 4411973 10 reactions GeneChip WT Terminal Labeling and Controls Kit Cat No 901525 10 reactions Additional GeneChip WT Terminal Labeling Kit 10 reactions Cat No 900670 is available separately • For 30 reactions WT Expression Kit Cat No 4411974 10 reactions GeneChip WT Terminal Labeling and Controls Kit Cat No 901524 10 reactions Documentation The following documentation is required for using the WT Expression Kit and the GeneChip WT Terminal Labeling and Controls Kit with Exon and Gene 1 0 ST Arrays • Protocol WT Expression Kit Cat No 4425209 • User Manual GeneChip WT Terminal Labeling and Hybridization Cat No 702808 • User Manual GeneChip Expression Wash Stain and Scan Cat No 702731 Related LinksGeneChip Hybridization Wash and Stain KitGeneChip WT Terminal Labeling and Controls KitatSNPtilx520433 Arabidopsis Cartridge Array
    https://www.bioz.com/result/controls kit/product/Thermo Fisher
    Average 99 stars, based on 119 article reviews
    Price from $9.99 to $1999.99
    controls kit - by Bioz Stars, 2021-01
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    Related Articles

    Amplification:

    Article Title: AGRONOMICS1: A New Resource for Arabidopsis Transcriptome Profiling
    Article Snippet: .. Amplified ChIP DNA was fragmented and labeled with the GeneChip WT Terminal Labeling kit (Affymetrix) according to the manufacturer's instructions. .. Fragmentation was confirmed using an RNA Nano 1000 kit on a 2100 Bioanalyzer lab-on-chip platform (Agilent), revealing an average fragment size of 88 nucleotides.

    Article Title: The balance of Id3 and E47 determines neural stem/precursor cell differentiation into astrocytes
    Article Snippet: .. Fragmentation and labeling of the amplified cDNAs was done with the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix). .. Labeled fragments were hybridized to Affymetrix GeneChip ST 1.0 arrays for 16 h at 45°C with 60 rpm in an Affymetrix Hybridization oven 645.

    In Vitro:

    Article Title: Expression of transcription factor grainyhead-like 2 is diminished in cervical cancer
    Article Snippet: .. This was then used to generate multiple copies of biotinylated cRNA by in vitro transcription with the GeneChip® WT Terminal Labeling and Controls Kit (Affymetrix). .. Biotinylated cRNA (10 μg) of each sample was hybridized to the Human Gene 1.1 ST Array Strip (Affymetrix) for 16 h at 43°C in the GeneAtlas Hybridization Station.

    Labeling:

    Article Title: Gene expression profiling analysis to investigate the role of remote ischemic postconditioning in ischemia-reperfusion injury in rats
    Article Snippet: .. Then, we used the Ambion WT Expression Kit (Affymetrix) and GeneChip WT Terminal Labeling and Controls Kit (Affymetrix) to obtain cDNA and cRNA products. .. Next, an Affymetrix Rat Gene 2.0 ST chip was used to hybridize the fragmented cRNA product.

    Article Title: Hypophosphatemia Regulates Molecular Mechanisms of Circadian Rhythm
    Article Snippet: .. 200 ng of RNA from each of the three mRNA pools was labeled with biotin using the Ambion WT Expression Kit (Life Technologies, Grand Island, NY) according to the manufacturer’s protocol, followed by the GeneChip WT Terminal Labeling and Controls Kit (Thermo Fisher, Waltham, MA). .. Labeled, fragmented DNA was hybridized to the Affymetrix Mouse Gene 1.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45 °C with rotation (60 rpm).

    Article Title: A Syngeneic ErbB2 Mammary Cancer Model for Preclinical Immunotherapy Trials
    Article Snippet: .. Biotinylated sense strand DNA targets were prepared from total RNA (100 ng) using the Ambion WT Expression Kit (Thermo Fisher Scientific) and Affymetrix GeneChip WT Terminal Labeling Kit. .. Briefly, double-stranded cDNA was prepared followed by in vitro transcription to generate antisense cRNA (aRNA), which was used as template for a second cycle cDNA synthesis in a dUTP-containing reaction mixture.

    Article Title: Expression of transcription factor grainyhead-like 2 is diminished in cervical cancer
    Article Snippet: .. This was then used to generate multiple copies of biotinylated cRNA by in vitro transcription with the GeneChip® WT Terminal Labeling and Controls Kit (Affymetrix). .. Biotinylated cRNA (10 μg) of each sample was hybridized to the Human Gene 1.1 ST Array Strip (Affymetrix) for 16 h at 43°C in the GeneAtlas Hybridization Station.

    Article Title: Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street? 1
    Article Snippet: .. Purified cDNA (5.5 μg) was fragmented and labeled using the GeneChip WT Terminal Labeling kit (Affymetrix), and fragmentation was verified using the Agilent 2100 Bioanalyzer. ..

    Article Title: AGRONOMICS1: A New Resource for Arabidopsis Transcriptome Profiling
    Article Snippet: .. Amplified ChIP DNA was fragmented and labeled with the GeneChip WT Terminal Labeling kit (Affymetrix) according to the manufacturer's instructions. .. Fragmentation was confirmed using an RNA Nano 1000 kit on a 2100 Bioanalyzer lab-on-chip platform (Agilent), revealing an average fragment size of 88 nucleotides.

    Article Title: The balance of Id3 and E47 determines neural stem/precursor cell differentiation into astrocytes
    Article Snippet: .. Fragmentation and labeling of the amplified cDNAs was done with the GeneChip WT Terminal Labeling and Controls Kit (Affymetrix). .. Labeled fragments were hybridized to Affymetrix GeneChip ST 1.0 arrays for 16 h at 45°C with 60 rpm in an Affymetrix Hybridization oven 645.

    Article Title: Polycomb group protein Suz12 is regulated by a novel miRNA-like small RNA
    Article Snippet: .. RNA samples were processed per manufacturers’ protocols using Ambion Whole-Transcript (WT) Expression kit, and Affymetrix GeneChip WT Terminal Labeling and Controls Reagent kit on Affymetrix equipment (Scanner 3000 7 G System). ..

    Purification:

    Article Title: Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street? 1
    Article Snippet: .. Purified cDNA (5.5 μg) was fragmented and labeled using the GeneChip WT Terminal Labeling kit (Affymetrix), and fragmentation was verified using the Agilent 2100 Bioanalyzer. ..

    Expressing:

    Article Title: Gene expression profiling analysis to investigate the role of remote ischemic postconditioning in ischemia-reperfusion injury in rats
    Article Snippet: .. Then, we used the Ambion WT Expression Kit (Affymetrix) and GeneChip WT Terminal Labeling and Controls Kit (Affymetrix) to obtain cDNA and cRNA products. .. Next, an Affymetrix Rat Gene 2.0 ST chip was used to hybridize the fragmented cRNA product.

    Article Title: Hypophosphatemia Regulates Molecular Mechanisms of Circadian Rhythm
    Article Snippet: .. 200 ng of RNA from each of the three mRNA pools was labeled with biotin using the Ambion WT Expression Kit (Life Technologies, Grand Island, NY) according to the manufacturer’s protocol, followed by the GeneChip WT Terminal Labeling and Controls Kit (Thermo Fisher, Waltham, MA). .. Labeled, fragmented DNA was hybridized to the Affymetrix Mouse Gene 1.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45 °C with rotation (60 rpm).

    Article Title: A Syngeneic ErbB2 Mammary Cancer Model for Preclinical Immunotherapy Trials
    Article Snippet: .. Biotinylated sense strand DNA targets were prepared from total RNA (100 ng) using the Ambion WT Expression Kit (Thermo Fisher Scientific) and Affymetrix GeneChip WT Terminal Labeling Kit. .. Briefly, double-stranded cDNA was prepared followed by in vitro transcription to generate antisense cRNA (aRNA), which was used as template for a second cycle cDNA synthesis in a dUTP-containing reaction mixture.

    Article Title: Polycomb group protein Suz12 is regulated by a novel miRNA-like small RNA
    Article Snippet: .. RNA samples were processed per manufacturers’ protocols using Ambion Whole-Transcript (WT) Expression kit, and Affymetrix GeneChip WT Terminal Labeling and Controls Reagent kit on Affymetrix equipment (Scanner 3000 7 G System). ..

    Chromatin Immunoprecipitation:

    Article Title: AGRONOMICS1: A New Resource for Arabidopsis Transcriptome Profiling
    Article Snippet: .. Amplified ChIP DNA was fragmented and labeled with the GeneChip WT Terminal Labeling kit (Affymetrix) according to the manufacturer's instructions. .. Fragmentation was confirmed using an RNA Nano 1000 kit on a 2100 Bioanalyzer lab-on-chip platform (Agilent), revealing an average fragment size of 88 nucleotides.

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    Thermo Fisher flow cytometry controls
    GJIC competences by flow <t>cytometry.</t> Percent communication calculated by the formula described in Methods for different time points has been represented for the cell lines used to develop this protocol. FBM ( A ), BT474 ( B ), and reference human cancer cell line, NT8e ( C ). ** P
    Flow Cytometry Controls, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry controls/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    85
    Thermo Fisher sybr green cells to ct control kit
    Expression of miR-125a, let-7e, and SP-AS in primary hepatocytes and hepatocellular carcinoma (HCC) cell lines HepG2 and HuH-7. Expression of the miRNAs ( a ) and SP-AS ( b ) was determined by TaqMan or <t>SYBR</t> Green <t>qPCR</t> assays, respectively; data from triplicate analyses were reported as mean ± SD of 2 Δ Ct values. Values for SP-AS expression are given multiplied by 10 4 .
    Sybr Green Cells To Ct Control Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green cells to ct control kit/product/Thermo Fisher
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
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    85/100 stars
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    99
    Thermo Fisher pierce green renilla lentiluc packaging kit and lentiviral control particles
    There is a reciprocal regulation between miR-375 and MALAT1 in cervical cancer cells. A. Predicted three binding sites between MALAT1 and miR-375. WT: wild type sequence; MT: the designed mutant sequence without binding sites. B and D. QRT-PCR analysis of miR-375 (B) and MALAT1 (D) expression in SiHa and CaSki cells after transfection of miR-375 mimics. C and E. QRT-PCR analysis of MALAT1 (C) and miR-375 (E) expression in SiHa and CaSki cells after infected with MALAT1 expression <t>lentiviral</t> particles. F-H. Dual luciferase assay of the inhibiting effect of miR-375 mimics on <t>Renilla</t> luciferase expression in Hela cells co-transfected with pmirGLO-MALAT1-WT1 or pmirGLO-MALAT1-MT1 (F), pmirGLO-MALAT1-WT2 or pmirGLO-MALAT1-MT2 (G), pmirGLO-MALAT1-WT3 or pmirGLO-MALAT1-MT3, ** p
    Pierce Green Renilla Lentiluc Packaging Kit And Lentiviral Control Particles, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pierce green renilla lentiluc packaging kit and lentiviral control particles/product/Thermo Fisher
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    Image Search Results


    GJIC competences by flow cytometry. Percent communication calculated by the formula described in Methods for different time points has been represented for the cell lines used to develop this protocol. FBM ( A ), BT474 ( B ), and reference human cancer cell line, NT8e ( C ). ** P

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: An Assay to Assess Gap Junction Communication in Cell Lines

    doi: 10.7171/jbt.19-3001-001

    Figure Lengend Snippet: GJIC competences by flow cytometry. Percent communication calculated by the formula described in Methods for different time points has been represented for the cell lines used to develop this protocol. FBM ( A ), BT474 ( B ), and reference human cancer cell line, NT8e ( C ). ** P

    Article Snippet: Cells were acquired on a BD FACSAria (BD Biosciences, San Jose, CA, USA) at different time points: 0, 3, 4, 5, 16, and 24 h. The flow cytometry controls were unlabeled, only calcein and only DiD (V22887; Thermo Fisher Scientific), and labeled cells.

    Techniques: Flow Cytometry, Cytometry

    Expression of miR-125a, let-7e, and SP-AS in primary hepatocytes and hepatocellular carcinoma (HCC) cell lines HepG2 and HuH-7. Expression of the miRNAs ( a ) and SP-AS ( b ) was determined by TaqMan or SYBR Green qPCR assays, respectively; data from triplicate analyses were reported as mean ± SD of 2 Δ Ct values. Values for SP-AS expression are given multiplied by 10 4 .

    Journal: International Journal of Molecular Sciences

    Article Title: A Novel ceRNA Regulatory Network Involving the Long Non-Coding Antisense RNA SPACA6P-AS, miR-125a and its mRNA Targets in Hepatocarcinoma Cells

    doi: 10.3390/ijms21145068

    Figure Lengend Snippet: Expression of miR-125a, let-7e, and SP-AS in primary hepatocytes and hepatocellular carcinoma (HCC) cell lines HepG2 and HuH-7. Expression of the miRNAs ( a ) and SP-AS ( b ) was determined by TaqMan or SYBR Green qPCR assays, respectively; data from triplicate analyses were reported as mean ± SD of 2 Δ Ct values. Values for SP-AS expression are given multiplied by 10 4 .

    Article Snippet: Then standard SYBR Green Real-time qPCR assays were performed with the following primers: SP-AS, 5′-CAAATGTCATGCTCTGGAGGA-3′ and 5′-CCTGAGACCCTTTAACCTGTG-3′; Lin28b, 5′-AGAAAAGAAAACCAAAGGGAGATAG-3′ and 5′-GAGGTAGACATACTTCCTTAGCATGA-3′; MMP11, 5′-TCCTGACTTCTTT GGCTGTG-3′ and 5′-CCATGGGTCTCTAGCCTGAT-3′ [ ]; SIRT-7, 5′-GTCTGCATGAGCAGAAGCTG-3′ and 5′-GGAACGCAGGA GGTACAGAC-3′; Zbtb7a, 5′-ACGAGTGCAACATCTGCAAG-3′ and 5′-GGTCGTAGTTGTGGGCAAAG-3′ [ ]; CDC25B, 5′-TCAAGATGCCATGGAAGCCC-3′ and 5′-TCCATCTTCCGGTCAGGACT-3′ [ ]; HMGA2, 5′- AAGCAGCAGCAAGAACCAAC-3′ and 5′- GCCTCTTGGCCGTTTTTCTC-3′ [ ]; CCND1, 5′-TGGCGTTTCCCAGAG TCATC-3′ and 5′-AAGGAAGGGGCAGGGGATAA-3′; GAPDH (reference transcript), 5′-GAAGGTGAAGGTCGGAGTC-3′ and 5′-GAAGATGGTGATGGGATTT-3′.

    Techniques: Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction

    There is a reciprocal regulation between miR-375 and MALAT1 in cervical cancer cells. A. Predicted three binding sites between MALAT1 and miR-375. WT: wild type sequence; MT: the designed mutant sequence without binding sites. B and D. QRT-PCR analysis of miR-375 (B) and MALAT1 (D) expression in SiHa and CaSki cells after transfection of miR-375 mimics. C and E. QRT-PCR analysis of MALAT1 (C) and miR-375 (E) expression in SiHa and CaSki cells after infected with MALAT1 expression lentiviral particles. F-H. Dual luciferase assay of the inhibiting effect of miR-375 mimics on Renilla luciferase expression in Hela cells co-transfected with pmirGLO-MALAT1-WT1 or pmirGLO-MALAT1-MT1 (F), pmirGLO-MALAT1-WT2 or pmirGLO-MALAT1-MT2 (G), pmirGLO-MALAT1-WT3 or pmirGLO-MALAT1-MT3, ** p

    Journal: PLoS ONE

    Article Title: MiR-375 Is Epigenetically Downregulated by HPV-16 E6 Mediated DNMT1 Upregulation and Modulates EMT of Cervical Cancer Cells by Suppressing lncRNA MALAT1

    doi: 10.1371/journal.pone.0163460

    Figure Lengend Snippet: There is a reciprocal regulation between miR-375 and MALAT1 in cervical cancer cells. A. Predicted three binding sites between MALAT1 and miR-375. WT: wild type sequence; MT: the designed mutant sequence without binding sites. B and D. QRT-PCR analysis of miR-375 (B) and MALAT1 (D) expression in SiHa and CaSki cells after transfection of miR-375 mimics. C and E. QRT-PCR analysis of MALAT1 (C) and miR-375 (E) expression in SiHa and CaSki cells after infected with MALAT1 expression lentiviral particles. F-H. Dual luciferase assay of the inhibiting effect of miR-375 mimics on Renilla luciferase expression in Hela cells co-transfected with pmirGLO-MALAT1-WT1 or pmirGLO-MALAT1-MT1 (F), pmirGLO-MALAT1-WT2 or pmirGLO-MALAT1-MT2 (G), pmirGLO-MALAT1-WT3 or pmirGLO-MALAT1-MT3, ** p

    Article Snippet: Lentiviral particles were produced by co-transfecting expression vector pLV4-MALAT1 with viral particle packaging helper vector into 293T cells.

    Techniques: Binding Assay, Sequencing, Mutagenesis, Quantitative RT-PCR, Expressing, Transfection, Infection, Luciferase