Structured Review

System Biosciences Inc control vector pmirna1 gfp
a A luciferase reporter expression assay was performed to examine whether miR-520d-5p actually targets TEAD1 and GATAD2B . Out of the more than ten predicted binding sites in the 3′UTRs of TEAD1 and GATAD2B , two sequences [TTACAAG and TACAAAG in the 3′UTR of TEAD1 ( top ) or GATAD2B ( bottom )] were chosen to test for miR-520d-5p binding. These sites were predicted based on the four databases described above. b A luciferase reporter expression assay revealed that miR-520d-5p bound to both the 3′UTR of TEAD1 and the 3′UTR of GATAD2B . In contrast, a mismatch of miR-520d-5p (a control for standardizing data) and a scramble group did not significantly bind to the 3′UTR sites, resulting in a failure to induce luciferase expression. Using a luciferase reporter expression assay, the potent sites of miR-520d-5p binding to the 3′UTR were identified and representatively shown at base pairs 4,569–4,581 and 7,463–7,776 in the 3′UTR of TEAD1 and 3,622–3,639 in the 3′UTR of GATAD2B , respectively. The minus signs (−) in the synthesized miR-520d-5p sequence represent the mismatches in the synthesized miR-520d-3p, and the minus signs in the control vector <t>pMIRNA1/GFP</t> sequence represent the mismatch sequences within miR-520d-5p that was expressed from pMIRNA1-520d-5p/GFP ( n = 4). The data were analyzed by t test (* P
Control Vector Pmirna1 Gfp, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/control vector pmirna1 gfp/product/System Biosciences Inc
Average 86 stars, based on 2 article reviews
Price from $9.99 to $1999.99
control vector pmirna1 gfp - by Bioz Stars, 2020-08
86/100 stars

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1) Product Images from "Hsa-miR-520d Converts Fibroblasts into CD105+ Populations"

Article Title: Hsa-miR-520d Converts Fibroblasts into CD105+ Populations

Journal: Drugs in R & D

doi: 10.1007/s40268-014-0064-6

a A luciferase reporter expression assay was performed to examine whether miR-520d-5p actually targets TEAD1 and GATAD2B . Out of the more than ten predicted binding sites in the 3′UTRs of TEAD1 and GATAD2B , two sequences [TTACAAG and TACAAAG in the 3′UTR of TEAD1 ( top ) or GATAD2B ( bottom )] were chosen to test for miR-520d-5p binding. These sites were predicted based on the four databases described above. b A luciferase reporter expression assay revealed that miR-520d-5p bound to both the 3′UTR of TEAD1 and the 3′UTR of GATAD2B . In contrast, a mismatch of miR-520d-5p (a control for standardizing data) and a scramble group did not significantly bind to the 3′UTR sites, resulting in a failure to induce luciferase expression. Using a luciferase reporter expression assay, the potent sites of miR-520d-5p binding to the 3′UTR were identified and representatively shown at base pairs 4,569–4,581 and 7,463–7,776 in the 3′UTR of TEAD1 and 3,622–3,639 in the 3′UTR of GATAD2B , respectively. The minus signs (−) in the synthesized miR-520d-5p sequence represent the mismatches in the synthesized miR-520d-3p, and the minus signs in the control vector pMIRNA1/GFP sequence represent the mismatch sequences within miR-520d-5p that was expressed from pMIRNA1-520d-5p/GFP ( n = 4). The data were analyzed by t test (* P
Figure Legend Snippet: a A luciferase reporter expression assay was performed to examine whether miR-520d-5p actually targets TEAD1 and GATAD2B . Out of the more than ten predicted binding sites in the 3′UTRs of TEAD1 and GATAD2B , two sequences [TTACAAG and TACAAAG in the 3′UTR of TEAD1 ( top ) or GATAD2B ( bottom )] were chosen to test for miR-520d-5p binding. These sites were predicted based on the four databases described above. b A luciferase reporter expression assay revealed that miR-520d-5p bound to both the 3′UTR of TEAD1 and the 3′UTR of GATAD2B . In contrast, a mismatch of miR-520d-5p (a control for standardizing data) and a scramble group did not significantly bind to the 3′UTR sites, resulting in a failure to induce luciferase expression. Using a luciferase reporter expression assay, the potent sites of miR-520d-5p binding to the 3′UTR were identified and representatively shown at base pairs 4,569–4,581 and 7,463–7,776 in the 3′UTR of TEAD1 and 3,622–3,639 in the 3′UTR of GATAD2B , respectively. The minus signs (−) in the synthesized miR-520d-5p sequence represent the mismatches in the synthesized miR-520d-3p, and the minus signs in the control vector pMIRNA1/GFP sequence represent the mismatch sequences within miR-520d-5p that was expressed from pMIRNA1-520d-5p/GFP ( n = 4). The data were analyzed by t test (* P

Techniques Used: Luciferase, Expressing, Binding Assay, Synthesized, Sequencing, Plasmid Preparation

Related Articles

Luciferase:

Article Title: Hsa-miR-520d-5p promotes survival in human dermal fibroblasts exposed to a lethal dose of UV irradiation
Article Snippet: .. Synthesized hsa-miR-520d-5p (KOKEN, Tokyo, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector. .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) and pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as controls.

Article Title: Hsa-miR-520d Converts Fibroblasts into CD105+ Populations
Article Snippet: .. Synthesized miR-520d-5p (MBL, Nagoya, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector (firefly or Renilla ). .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) or pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as the controls.

Plasmid Preparation:

Article Title: Hsa-miR-520d-5p promotes survival in human dermal fibroblasts exposed to a lethal dose of UV irradiation
Article Snippet: .. Synthesized hsa-miR-520d-5p (KOKEN, Tokyo, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector. .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) and pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as controls.

Article Title: Hsa-miR-520d Converts Fibroblasts into CD105+ Populations
Article Snippet: .. Synthesized miR-520d-5p (MBL, Nagoya, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector (firefly or Renilla ). .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) or pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as the controls.

Expressing:

Article Title: Hsa-miR-520d-5p promotes survival in human dermal fibroblasts exposed to a lethal dose of UV irradiation
Article Snippet: .. Synthesized hsa-miR-520d-5p (KOKEN, Tokyo, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector. .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) and pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as controls.

Article Title: Hsa-miR-520d Converts Fibroblasts into CD105+ Populations
Article Snippet: .. Synthesized miR-520d-5p (MBL, Nagoya, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector (firefly or Renilla ). .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) or pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as the controls.

Synthesized:

Article Title: Hsa-miR-520d-5p promotes survival in human dermal fibroblasts exposed to a lethal dose of UV irradiation
Article Snippet: .. Synthesized hsa-miR-520d-5p (KOKEN, Tokyo, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector. .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) and pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as controls.

Article Title: Hsa-miR-520d Converts Fibroblasts into CD105+ Populations
Article Snippet: .. Synthesized miR-520d-5p (MBL, Nagoya, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector (firefly or Renilla ). .. Synthesized hsa-miR-520d-3p (MBL, Nagoya, Japan) or pLKO.1 (Addgene, Cambridge, MA, USA) with mismatch sequences were used as the controls.

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    System Biosciences Inc control vector pmirna1 gfp
    a A luciferase reporter expression assay was performed to examine whether miR-520d-5p actually targets TEAD1 and GATAD2B . Out of the more than ten predicted binding sites in the 3′UTRs of TEAD1 and GATAD2B , two sequences [TTACAAG and TACAAAG in the 3′UTR of TEAD1 ( top ) or GATAD2B ( bottom )] were chosen to test for miR-520d-5p binding. These sites were predicted based on the four databases described above. b A luciferase reporter expression assay revealed that miR-520d-5p bound to both the 3′UTR of TEAD1 and the 3′UTR of GATAD2B . In contrast, a mismatch of miR-520d-5p (a control for standardizing data) and a scramble group did not significantly bind to the 3′UTR sites, resulting in a failure to induce luciferase expression. Using a luciferase reporter expression assay, the potent sites of miR-520d-5p binding to the 3′UTR were identified and representatively shown at base pairs 4,569–4,581 and 7,463–7,776 in the 3′UTR of TEAD1 and 3,622–3,639 in the 3′UTR of GATAD2B , respectively. The minus signs (−) in the synthesized miR-520d-5p sequence represent the mismatches in the synthesized miR-520d-3p, and the minus signs in the control vector <t>pMIRNA1/GFP</t> sequence represent the mismatch sequences within miR-520d-5p that was expressed from pMIRNA1-520d-5p/GFP ( n = 4). The data were analyzed by t test (* P
    Control Vector Pmirna1 Gfp, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control vector pmirna1 gfp/product/System Biosciences Inc
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    control vector pmirna1 gfp - by Bioz Stars, 2020-08
    86/100 stars
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    a A luciferase reporter expression assay was performed to examine whether miR-520d-5p actually targets TEAD1 and GATAD2B . Out of the more than ten predicted binding sites in the 3′UTRs of TEAD1 and GATAD2B , two sequences [TTACAAG and TACAAAG in the 3′UTR of TEAD1 ( top ) or GATAD2B ( bottom )] were chosen to test for miR-520d-5p binding. These sites were predicted based on the four databases described above. b A luciferase reporter expression assay revealed that miR-520d-5p bound to both the 3′UTR of TEAD1 and the 3′UTR of GATAD2B . In contrast, a mismatch of miR-520d-5p (a control for standardizing data) and a scramble group did not significantly bind to the 3′UTR sites, resulting in a failure to induce luciferase expression. Using a luciferase reporter expression assay, the potent sites of miR-520d-5p binding to the 3′UTR were identified and representatively shown at base pairs 4,569–4,581 and 7,463–7,776 in the 3′UTR of TEAD1 and 3,622–3,639 in the 3′UTR of GATAD2B , respectively. The minus signs (−) in the synthesized miR-520d-5p sequence represent the mismatches in the synthesized miR-520d-3p, and the minus signs in the control vector pMIRNA1/GFP sequence represent the mismatch sequences within miR-520d-5p that was expressed from pMIRNA1-520d-5p/GFP ( n = 4). The data were analyzed by t test (* P

    Journal: Drugs in R & D

    Article Title: Hsa-miR-520d Converts Fibroblasts into CD105+ Populations

    doi: 10.1007/s40268-014-0064-6

    Figure Lengend Snippet: a A luciferase reporter expression assay was performed to examine whether miR-520d-5p actually targets TEAD1 and GATAD2B . Out of the more than ten predicted binding sites in the 3′UTRs of TEAD1 and GATAD2B , two sequences [TTACAAG and TACAAAG in the 3′UTR of TEAD1 ( top ) or GATAD2B ( bottom )] were chosen to test for miR-520d-5p binding. These sites were predicted based on the four databases described above. b A luciferase reporter expression assay revealed that miR-520d-5p bound to both the 3′UTR of TEAD1 and the 3′UTR of GATAD2B . In contrast, a mismatch of miR-520d-5p (a control for standardizing data) and a scramble group did not significantly bind to the 3′UTR sites, resulting in a failure to induce luciferase expression. Using a luciferase reporter expression assay, the potent sites of miR-520d-5p binding to the 3′UTR were identified and representatively shown at base pairs 4,569–4,581 and 7,463–7,776 in the 3′UTR of TEAD1 and 3,622–3,639 in the 3′UTR of GATAD2B , respectively. The minus signs (−) in the synthesized miR-520d-5p sequence represent the mismatches in the synthesized miR-520d-3p, and the minus signs in the control vector pMIRNA1/GFP sequence represent the mismatch sequences within miR-520d-5p that was expressed from pMIRNA1-520d-5p/GFP ( n = 4). The data were analyzed by t test (* P

    Article Snippet: Synthesized miR-520d-5p (MBL, Nagoya, Japan) or the control vector pMIRNA1/GFP (System Biosciences, Mountain View, CA, USA) were co-transfected into NHDF cells together with each prepared luciferase expression vector (firefly or Renilla ).

    Techniques: Luciferase, Expressing, Binding Assay, Synthesized, Sequencing, Plasmid Preparation