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GenePharma Company control vector pglv3 h1 gfp puro
Control Vector Pglv3 H1 Gfp Puro, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/control vector pglv3 h1 gfp puro/product/GenePharma Company
Average 89 stars, based on 8 article reviews
Price from $9.99 to $1999.99
control vector pglv3 h1 gfp puro - by Bioz Stars, 2020-08
89/100 stars

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Negative Control:

Article Title: miR-148a is Associated with Obesity and Modulates Adipocyte Differentiation of Mesenchymal Stem Cells through Wnt Signaling
Article Snippet: .. Plasmids and reporter assays The miR-148a lentiviral expression vector pGLV3-H1-GFP-puro-miR-148a, miR-148a sponge (four repeat complimentary sequences) lentiviral expression vector, and the negative control vector pGLV3-H1-GFP-puro were purchased from GenePharma (Shanghai, China). .. The miR-148a minigenes, including the upstream and downstream sequences of the pre-miRNA and open reading frame of CREB and Wnt1, were amplified by PCR and ligated into the Bam HI and Eco RI sites of pGLV3-H1-GFP-puro.

Expressing:

Article Title: miR-148a is Associated with Obesity and Modulates Adipocyte Differentiation of Mesenchymal Stem Cells through Wnt Signaling
Article Snippet: .. Plasmids and reporter assays The miR-148a lentiviral expression vector pGLV3-H1-GFP-puro-miR-148a, miR-148a sponge (four repeat complimentary sequences) lentiviral expression vector, and the negative control vector pGLV3-H1-GFP-puro were purchased from GenePharma (Shanghai, China). .. The miR-148a minigenes, including the upstream and downstream sequences of the pre-miRNA and open reading frame of CREB and Wnt1, were amplified by PCR and ligated into the Bam HI and Eco RI sites of pGLV3-H1-GFP-puro.

Plasmid Preparation:

Article Title: miR-148a is Associated with Obesity and Modulates Adipocyte Differentiation of Mesenchymal Stem Cells through Wnt Signaling
Article Snippet: .. Plasmids and reporter assays The miR-148a lentiviral expression vector pGLV3-H1-GFP-puro-miR-148a, miR-148a sponge (four repeat complimentary sequences) lentiviral expression vector, and the negative control vector pGLV3-H1-GFP-puro were purchased from GenePharma (Shanghai, China). .. The miR-148a minigenes, including the upstream and downstream sequences of the pre-miRNA and open reading frame of CREB and Wnt1, were amplified by PCR and ligated into the Bam HI and Eco RI sites of pGLV3-H1-GFP-puro.

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    GenePharma Company il 17rb mrna
    <t>IL-17E-IL-17RB</t> activation contributes to host defense by promoting the production of antibacterial protein Reg3a during the early-phase of H. pylori of infection. a Expression of human β-defensin (BD)-1, BD-2, BD-3, BD-4, regenerating family member (REG) 1 A, REG1B, REG3, and REG4 in AGS cells stimulated with IL-17E or IL-17B was compared ( n = 3).The heatmap was performed by the software HemI.1.0 based on the Quantitative RT-PCR values. Used black as the baseline of genes expression (baseline is defined as 0) and red represents higher expression. The color scale and fold change values are shown at the bottom right corner of the heatmap. b Expression of REG3 IL-17E- or IL-17B-stimulated AGS cells pre-treated with LV3-NC or LV3-shIL-17RB was compared ( n = 3). ( c and d ) Mouse regenerating family member (Reg) 3a <t>mRNA</t> expression ( c ) and Reg3a protein concentration ( d ) in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. were compared. Each dot represents one mouse. ( e and f ) Mouse Reg3a mRNA expression ( e ) and Reg3a protein concentration ( f ) in gastric mucosa of WT H. pylori -infected mice injected with IL-17B, IL-17E, IL-17B and IL-17E, or PBS control on day 9 p.i. were compared ( n = 5). * P
    Il 17rb Mrna, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 17rb mrna/product/GenePharma Company
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    il 17rb mrna - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    GenePharma Company lv3 shnc
    Morphological changes of glioma U251 cells 48 h post treatment, observed under inverted microscope (magnification 100 T). (a) Untreated U251 cells. (b) <t>LV3-shNC-transfected</t> U251 cells. (c) LV3-HmiR-27b-transfected U251 cells, representing the morphological changes
    Lv3 Shnc, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lv3 shnc/product/GenePharma Company
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lv3 shnc - by Bioz Stars, 2020-08
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    88
    GenePharma Company lentiviral vector expressing shrna targeting march7
    Silencing of <t>MARCH7</t> inhibited the invasion of cervical cancer HeLa cells. (A-C) Cervical cancer HeLa cells invasion ability was detected by Matrigel invasion assays. The invasion ability of LV3-shMARCH7-1 and LV3-shMARCH7-2 infected HeLa cells was decreased compared with LV3-NC infected cells (D-F). F-actin staining. Original magnification, −400. LV3-shMARCH7-1, <t>lentiviral</t> vector expressing <t>shRNA</t> targeting MARCH7; NC, negative control; LV, lentiviral vector.
    Lentiviral Vector Expressing Shrna Targeting March7, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral vector expressing shrna targeting march7/product/GenePharma Company
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    lentiviral vector expressing shrna targeting march7 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    IL-17E-IL-17RB activation contributes to host defense by promoting the production of antibacterial protein Reg3a during the early-phase of H. pylori of infection. a Expression of human β-defensin (BD)-1, BD-2, BD-3, BD-4, regenerating family member (REG) 1 A, REG1B, REG3, and REG4 in AGS cells stimulated with IL-17E or IL-17B was compared ( n = 3).The heatmap was performed by the software HemI.1.0 based on the Quantitative RT-PCR values. Used black as the baseline of genes expression (baseline is defined as 0) and red represents higher expression. The color scale and fold change values are shown at the bottom right corner of the heatmap. b Expression of REG3 IL-17E- or IL-17B-stimulated AGS cells pre-treated with LV3-NC or LV3-shIL-17RB was compared ( n = 3). ( c and d ) Mouse regenerating family member (Reg) 3a mRNA expression ( c ) and Reg3a protein concentration ( d ) in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. were compared. Each dot represents one mouse. ( e and f ) Mouse Reg3a mRNA expression ( e ) and Reg3a protein concentration ( f ) in gastric mucosa of WT H. pylori -infected mice injected with IL-17B, IL-17E, IL-17B and IL-17E, or PBS control on day 9 p.i. were compared ( n = 5). * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: IL-17E-IL-17RB activation contributes to host defense by promoting the production of antibacterial protein Reg3a during the early-phase of H. pylori of infection. a Expression of human β-defensin (BD)-1, BD-2, BD-3, BD-4, regenerating family member (REG) 1 A, REG1B, REG3, and REG4 in AGS cells stimulated with IL-17E or IL-17B was compared ( n = 3).The heatmap was performed by the software HemI.1.0 based on the Quantitative RT-PCR values. Used black as the baseline of genes expression (baseline is defined as 0) and red represents higher expression. The color scale and fold change values are shown at the bottom right corner of the heatmap. b Expression of REG3 IL-17E- or IL-17B-stimulated AGS cells pre-treated with LV3-NC or LV3-shIL-17RB was compared ( n = 3). ( c and d ) Mouse regenerating family member (Reg) 3a mRNA expression ( c ) and Reg3a protein concentration ( d ) in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. were compared. Each dot represents one mouse. ( e and f ) Mouse Reg3a mRNA expression ( e ) and Reg3a protein concentration ( f ) in gastric mucosa of WT H. pylori -infected mice injected with IL-17B, IL-17E, IL-17B and IL-17E, or PBS control on day 9 p.i. were compared ( n = 5). * P

    Article Snippet: Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Activation Assay, Infection, Expressing, Software, Quantitative RT-PCR, Protein Concentration, Mouse Assay, Injection

    IL-17RB is decreased in gastric mucosa of H. pylori -infected patients and mice. a IL-17RB mRNA expression in gastric mucosa of H. pylori -infected ( n = 80) and uninfected donors ( n = 16) were compared. b The correlation between IL-17RB mRNA expression and H. pylori colonization in gastric mucosa of H. pylori -infected donors was analyzed. c IL-17RB mRNA expression in gastric mucosa of uninfected ( n = 16), cagA + H. pylori -infected ( n = 67), cagA − and H. pylori -infected ( n = 13) donors was compared. d Dynamic changes of IL-17RB mRNA expression and IL-17RB mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, Δ cagA -infected, and uninfected mice. n = 5 per group per time point in ( d ). e IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by IHC. Scale bars: 100 μm. f IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by Western blot. g IL-17RB mRNA expression or IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected isolated primary gastric mucosa tissues (MOI = 100, 24 h) from uninfected donors were compared ( n = 3) analyzed by real time PCR or Western blot. * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: IL-17RB is decreased in gastric mucosa of H. pylori -infected patients and mice. a IL-17RB mRNA expression in gastric mucosa of H. pylori -infected ( n = 80) and uninfected donors ( n = 16) were compared. b The correlation between IL-17RB mRNA expression and H. pylori colonization in gastric mucosa of H. pylori -infected donors was analyzed. c IL-17RB mRNA expression in gastric mucosa of uninfected ( n = 16), cagA + H. pylori -infected ( n = 67), cagA − and H. pylori -infected ( n = 13) donors was compared. d Dynamic changes of IL-17RB mRNA expression and IL-17RB mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, Δ cagA -infected, and uninfected mice. n = 5 per group per time point in ( d ). e IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by IHC. Scale bars: 100 μm. f IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by Western blot. g IL-17RB mRNA expression or IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected isolated primary gastric mucosa tissues (MOI = 100, 24 h) from uninfected donors were compared ( n = 3) analyzed by real time PCR or Western blot. * P

    Article Snippet: Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Infection, Mouse Assay, Expressing, Immunohistochemistry, Western Blot, Isolation, Real-time Polymerase Chain Reaction

    H. pylori-stimulated gastric epithelial cells (GECs) to downregulate IL-17RB. a Representative immunofluorescence staining images showing IL-17RB-expressing (red) CD326 + GECs (green) in gastric mucosa of uninfected donors. Scale bars: 100 microns. b IL-17R family member mRNA expression in H. pylori -infected and uninfected AGS cells was compared ( n = 3). The heatmap was generated by the software HemI.1.0 based on the Quantitative RT-PCR values, black is used as the baseline of genes expression (baseline is defined as 0) and green represents lower expression. The color scale and fold change values are shown at the bottom of the heatmap. c IL-17RB mRNA expression in H. pylori -infected and uninfected AGS cells or HGC-27 cells (MOI = 100, 12 or 24 h) was compared ( n = 3). d IL-17RB mRNA expression and IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected AGS cells or HGC-27 cells (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot. e IL-17RB mRNA and IL-17RB protein expression in H. pylori -infected and uninfected AGS cells with different MOI (24 h) or at different time point (MOI = 100) were analyzed by real-time PCR and Western blot ( n = 3). f IL-17RB mRNA and IL-17RB protein expression in WT H. pylori -infected, ΔcagA -infected, and uninfected primary GECs (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot ( n = 3). g AGS cells were pre-treated with Wortmannin (a PI3K inhibitor) and then stimulated with WT H. pylori (MOI = 100) for 24 h. AKT and p-AKT and IL-17RB proteins were analyzed by Western blots. * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: H. pylori-stimulated gastric epithelial cells (GECs) to downregulate IL-17RB. a Representative immunofluorescence staining images showing IL-17RB-expressing (red) CD326 + GECs (green) in gastric mucosa of uninfected donors. Scale bars: 100 microns. b IL-17R family member mRNA expression in H. pylori -infected and uninfected AGS cells was compared ( n = 3). The heatmap was generated by the software HemI.1.0 based on the Quantitative RT-PCR values, black is used as the baseline of genes expression (baseline is defined as 0) and green represents lower expression. The color scale and fold change values are shown at the bottom of the heatmap. c IL-17RB mRNA expression in H. pylori -infected and uninfected AGS cells or HGC-27 cells (MOI = 100, 12 or 24 h) was compared ( n = 3). d IL-17RB mRNA expression and IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected AGS cells or HGC-27 cells (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot. e IL-17RB mRNA and IL-17RB protein expression in H. pylori -infected and uninfected AGS cells with different MOI (24 h) or at different time point (MOI = 100) were analyzed by real-time PCR and Western blot ( n = 3). f IL-17RB mRNA and IL-17RB protein expression in WT H. pylori -infected, ΔcagA -infected, and uninfected primary GECs (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot ( n = 3). g AGS cells were pre-treated with Wortmannin (a PI3K inhibitor) and then stimulated with WT H. pylori (MOI = 100) for 24 h. AKT and p-AKT and IL-17RB proteins were analyzed by Western blots. * P

    Article Snippet: Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Immunofluorescence, Staining, Expressing, Infection, Generated, Software, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot

    IL-17E, a ligand of IL-17RB, is decreased in gastric mucosa during the early-phase of H. pylori infection. a Dynamic change of IL-17E mRNA expression and IL-17E mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in ( a ). b Dynamic change of IL-17B mRNA expression in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in b. ( c ) IL-17E protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. d IL-17B protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: IL-17E, a ligand of IL-17RB, is decreased in gastric mucosa during the early-phase of H. pylori infection. a Dynamic change of IL-17E mRNA expression and IL-17E mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in ( a ). b Dynamic change of IL-17B mRNA expression in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in b. ( c ) IL-17E protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. d IL-17B protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. * P

    Article Snippet: Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Infection, Expressing, Mouse Assay, Protein Concentration

    IL-17E-IL-17RB activation contributes to host defense by promoting the production of antibacterial protein Reg3a during the early-phase of H. pylori of infection. a Expression of human β-defensin (BD)-1, BD-2, BD-3, BD-4, regenerating family member (REG) 1 A, REG1B, REG3, and REG4 in AGS cells stimulated with IL-17E or IL-17B was compared ( n = 3).The heatmap was performed by the software HemI.1.0 based on the Quantitative RT-PCR values. Used black as the baseline of genes expression (baseline is defined as 0) and red represents higher expression. The color scale and fold change values are shown at the bottom right corner of the heatmap. b Expression of REG3 IL-17E- or IL-17B-stimulated AGS cells pre-treated with LV3-NC or LV3-shIL-17RB was compared ( n = 3). ( c and d ) Mouse regenerating family member (Reg) 3a mRNA expression ( c ) and Reg3a protein concentration ( d ) in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. were compared. Each dot represents one mouse. ( e and f ) Mouse Reg3a mRNA expression ( e ) and Reg3a protein concentration ( f ) in gastric mucosa of WT H. pylori -infected mice injected with IL-17B, IL-17E, IL-17B and IL-17E, or PBS control on day 9 p.i. were compared ( n = 5). * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: IL-17E-IL-17RB activation contributes to host defense by promoting the production of antibacterial protein Reg3a during the early-phase of H. pylori of infection. a Expression of human β-defensin (BD)-1, BD-2, BD-3, BD-4, regenerating family member (REG) 1 A, REG1B, REG3, and REG4 in AGS cells stimulated with IL-17E or IL-17B was compared ( n = 3).The heatmap was performed by the software HemI.1.0 based on the Quantitative RT-PCR values. Used black as the baseline of genes expression (baseline is defined as 0) and red represents higher expression. The color scale and fold change values are shown at the bottom right corner of the heatmap. b Expression of REG3 IL-17E- or IL-17B-stimulated AGS cells pre-treated with LV3-NC or LV3-shIL-17RB was compared ( n = 3). ( c and d ) Mouse regenerating family member (Reg) 3a mRNA expression ( c ) and Reg3a protein concentration ( d ) in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. were compared. Each dot represents one mouse. ( e and f ) Mouse Reg3a mRNA expression ( e ) and Reg3a protein concentration ( f ) in gastric mucosa of WT H. pylori -infected mice injected with IL-17B, IL-17E, IL-17B and IL-17E, or PBS control on day 9 p.i. were compared ( n = 5). * P

    Article Snippet: Lentiviral knockdown of IL-17RB in AGS cells Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Activation Assay, Infection, Expressing, Software, Quantitative RT-PCR, Protein Concentration, Mouse Assay, Injection

    IL-17RB is decreased in gastric mucosa of H. pylori -infected patients and mice. a IL-17RB mRNA expression in gastric mucosa of H. pylori -infected ( n = 80) and uninfected donors ( n = 16) were compared. b The correlation between IL-17RB mRNA expression and H. pylori colonization in gastric mucosa of H. pylori -infected donors was analyzed. c IL-17RB mRNA expression in gastric mucosa of uninfected ( n = 16), cagA + H. pylori -infected ( n = 67), cagA − and H. pylori -infected ( n = 13) donors was compared. d Dynamic changes of IL-17RB mRNA expression and IL-17RB mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, Δ cagA -infected, and uninfected mice. n = 5 per group per time point in ( d ). e IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by IHC. Scale bars: 100 μm. f IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by Western blot. g IL-17RB mRNA expression or IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected isolated primary gastric mucosa tissues (MOI = 100, 24 h) from uninfected donors were compared ( n = 3) analyzed by real time PCR or Western blot. * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: IL-17RB is decreased in gastric mucosa of H. pylori -infected patients and mice. a IL-17RB mRNA expression in gastric mucosa of H. pylori -infected ( n = 80) and uninfected donors ( n = 16) were compared. b The correlation between IL-17RB mRNA expression and H. pylori colonization in gastric mucosa of H. pylori -infected donors was analyzed. c IL-17RB mRNA expression in gastric mucosa of uninfected ( n = 16), cagA + H. pylori -infected ( n = 67), cagA − and H. pylori -infected ( n = 13) donors was compared. d Dynamic changes of IL-17RB mRNA expression and IL-17RB mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, Δ cagA -infected, and uninfected mice. n = 5 per group per time point in ( d ). e IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by IHC. Scale bars: 100 μm. f IL-17RB protein in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 9 p.i. were analyzed by Western blot. g IL-17RB mRNA expression or IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected isolated primary gastric mucosa tissues (MOI = 100, 24 h) from uninfected donors were compared ( n = 3) analyzed by real time PCR or Western blot. * P

    Article Snippet: Lentiviral knockdown of IL-17RB in AGS cells Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Infection, Mouse Assay, Expressing, Immunohistochemistry, Western Blot, Isolation, Real-time Polymerase Chain Reaction

    H. pylori-stimulated gastric epithelial cells (GECs) to downregulate IL-17RB. a Representative immunofluorescence staining images showing IL-17RB-expressing (red) CD326 + GECs (green) in gastric mucosa of uninfected donors. Scale bars: 100 microns. b IL-17R family member mRNA expression in H. pylori -infected and uninfected AGS cells was compared ( n = 3). The heatmap was generated by the software HemI.1.0 based on the Quantitative RT-PCR values, black is used as the baseline of genes expression (baseline is defined as 0) and green represents lower expression. The color scale and fold change values are shown at the bottom of the heatmap. c IL-17RB mRNA expression in H. pylori -infected and uninfected AGS cells or HGC-27 cells (MOI = 100, 12 or 24 h) was compared ( n = 3). d IL-17RB mRNA expression and IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected AGS cells or HGC-27 cells (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot. e IL-17RB mRNA and IL-17RB protein expression in H. pylori -infected and uninfected AGS cells with different MOI (24 h) or at different time point (MOI = 100) were analyzed by real-time PCR and Western blot ( n = 3). f IL-17RB mRNA and IL-17RB protein expression in WT H. pylori -infected, ΔcagA -infected, and uninfected primary GECs (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot ( n = 3). g AGS cells were pre-treated with Wortmannin (a PI3K inhibitor) and then stimulated with WT H. pylori (MOI = 100) for 24 h. AKT and p-AKT and IL-17RB proteins were analyzed by Western blots. * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: H. pylori-stimulated gastric epithelial cells (GECs) to downregulate IL-17RB. a Representative immunofluorescence staining images showing IL-17RB-expressing (red) CD326 + GECs (green) in gastric mucosa of uninfected donors. Scale bars: 100 microns. b IL-17R family member mRNA expression in H. pylori -infected and uninfected AGS cells was compared ( n = 3). The heatmap was generated by the software HemI.1.0 based on the Quantitative RT-PCR values, black is used as the baseline of genes expression (baseline is defined as 0) and green represents lower expression. The color scale and fold change values are shown at the bottom of the heatmap. c IL-17RB mRNA expression in H. pylori -infected and uninfected AGS cells or HGC-27 cells (MOI = 100, 12 or 24 h) was compared ( n = 3). d IL-17RB mRNA expression and IL-17RB protein in WT H. pylori -infected, ΔcagA -infected, and uninfected AGS cells or HGC-27 cells (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot. e IL-17RB mRNA and IL-17RB protein expression in H. pylori -infected and uninfected AGS cells with different MOI (24 h) or at different time point (MOI = 100) were analyzed by real-time PCR and Western blot ( n = 3). f IL-17RB mRNA and IL-17RB protein expression in WT H. pylori -infected, ΔcagA -infected, and uninfected primary GECs (MOI = 100, 24 h) were analyzed by real-time PCR and Western blot ( n = 3). g AGS cells were pre-treated with Wortmannin (a PI3K inhibitor) and then stimulated with WT H. pylori (MOI = 100) for 24 h. AKT and p-AKT and IL-17RB proteins were analyzed by Western blots. * P

    Article Snippet: Lentiviral knockdown of IL-17RB in AGS cells Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Immunofluorescence, Staining, Expressing, Infection, Generated, Software, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot

    IL-17E, a ligand of IL-17RB, is decreased in gastric mucosa during the early-phase of H. pylori infection. a Dynamic change of IL-17E mRNA expression and IL-17E mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in ( a ). b Dynamic change of IL-17B mRNA expression in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in b. ( c ) IL-17E protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. d IL-17B protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. * P

    Journal: Cell Death & Disease

    Article Title: Decreased IL-17RB expression impairs CD11b+CD11c− myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection

    doi: 10.1038/s41419-019-1312-z

    Figure Lengend Snippet: IL-17E, a ligand of IL-17RB, is decreased in gastric mucosa during the early-phase of H. pylori infection. a Dynamic change of IL-17E mRNA expression and IL-17E mRNA expression on day 7 or 9 p.i. in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in ( a ). b Dynamic change of IL-17B mRNA expression in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice. n = 5 per group per time point in b. ( c ) IL-17E protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. d IL-17B protein concentration in gastric mucosa of WT H. pylori -infected, ΔcagA -infected, and uninfected mice on day 7 or 9 p.i. was compared. Each dot represents one mouse. * P

    Article Snippet: Lentiviral knockdown of IL-17RB in AGS cells Lentiviral particles containing short hairpin RNA (shRNA) targeting IL-17RB mRNA (named as LV3-shIL-17RB) and its negative control containing a non-targeting RNA sequence (named as LV3-NC) were designed, constructed, amplified, and purified by GenePharma (Shanghai, China).

    Techniques: Infection, Expressing, Mouse Assay, Protein Concentration

    Morphological changes of glioma U251 cells 48 h post treatment, observed under inverted microscope (magnification 100 T). (a) Untreated U251 cells. (b) LV3-shNC-transfected U251 cells. (c) LV3-HmiR-27b-transfected U251 cells, representing the morphological changes

    Journal: Experimental Biology and Medicine

    Article Title: Hsa-miR-27b is up-regulated in cytomegalovirus-infected human glioma cells, targets engrailed-2 and inhibits its expression

    doi: 10.1177/1535370217699535

    Figure Lengend Snippet: Morphological changes of glioma U251 cells 48 h post treatment, observed under inverted microscope (magnification 100 T). (a) Untreated U251 cells. (b) LV3-shNC-transfected U251 cells. (c) LV3-HmiR-27b-transfected U251 cells, representing the morphological changes

    Article Snippet: Second, 4 µg of the plasmid LV3-HmiR-27b or LV3-shNC were isolated and mixed with 15 μl transfection reagent of RNAi-Mate (GenePharma, Shanghai, China).

    Techniques: Inverted Microscopy, Transfection

    The EN2 protein expression levels in groups subjected to different treatments. (a) Western blot detection of EN2 in different groups. β-actin was used as an internal control of each sample and (b) comparison of the relative expression levels of EN2 in different groups. Mock control: The group of untreated U251 cells; shNC: The negative control group of U251 cells transfected with LV3-shNC; miR-27b: The treatment group of U251 cells transfected with LV3-HmiR-27b EN2: engrailed-2 Note: *refers to P

    Journal: Experimental Biology and Medicine

    Article Title: Hsa-miR-27b is up-regulated in cytomegalovirus-infected human glioma cells, targets engrailed-2 and inhibits its expression

    doi: 10.1177/1535370217699535

    Figure Lengend Snippet: The EN2 protein expression levels in groups subjected to different treatments. (a) Western blot detection of EN2 in different groups. β-actin was used as an internal control of each sample and (b) comparison of the relative expression levels of EN2 in different groups. Mock control: The group of untreated U251 cells; shNC: The negative control group of U251 cells transfected with LV3-shNC; miR-27b: The treatment group of U251 cells transfected with LV3-HmiR-27b EN2: engrailed-2 Note: *refers to P

    Article Snippet: Second, 4 µg of the plasmid LV3-HmiR-27b or LV3-shNC were isolated and mixed with 15 μl transfection reagent of RNAi-Mate (GenePharma, Shanghai, China).

    Techniques: Expressing, Western Blot, Negative Control, Transfection

    Silencing of MARCH7 inhibited the invasion of cervical cancer HeLa cells. (A-C) Cervical cancer HeLa cells invasion ability was detected by Matrigel invasion assays. The invasion ability of LV3-shMARCH7-1 and LV3-shMARCH7-2 infected HeLa cells was decreased compared with LV3-NC infected cells (D-F). F-actin staining. Original magnification, −400. LV3-shMARCH7-1, lentiviral vector expressing shRNA targeting MARCH7; NC, negative control; LV, lentiviral vector.

    Journal: Oncology Letters

    Article Title: Ubiquitin E3 Ligase MARCH7 promotes proliferation and invasion of cervical cancer cells through VAV2-RAC1-CDC42 pathway

    doi: 10.3892/ol.2018.8908

    Figure Lengend Snippet: Silencing of MARCH7 inhibited the invasion of cervical cancer HeLa cells. (A-C) Cervical cancer HeLa cells invasion ability was detected by Matrigel invasion assays. The invasion ability of LV3-shMARCH7-1 and LV3-shMARCH7-2 infected HeLa cells was decreased compared with LV3-NC infected cells (D-F). F-actin staining. Original magnification, −400. LV3-shMARCH7-1, lentiviral vector expressing shRNA targeting MARCH7; NC, negative control; LV, lentiviral vector.

    Article Snippet: Lentiviral vector expressing shRNA targeting MARCH7 (named LV3-shMarch7-1 and LV3-shMarch7-2) were provided from Genepharma Co., Ltd..

    Techniques: Infection, Staining, Plasmid Preparation, Expressing, shRNA, Negative Control

    MARCH7 regulated VAV2-RAC1-CDC42 pathway. (A) The interaction between MARCH7 and VAV2 was identified by Co-IP assay. Cells were co-transfected with Flag-VAV2 and HA-MARCH7, and control group was established simultaneously, cells were then harvested 24 h later. Anti-HA antibodies were used to pull the interaction protein. Then, they were detected by anti-Flag antibodies. Results showed that Flag bands could not be detected in the cells transfected with Flag-VAV2 (lane 1) or HA-MARCH7 (lane 3) only. However, it can be detected in cells co-transfected with both Flag-VAV2 and HA-MARCH7 (lane 2), which indicated that there existed interaction between MARCH7 and VAV2 in vivo . (B) The expression of VAV2, RAC1 and CDC42 were determined by western blot analysis. The VAV2, RAC1 and CDC42 expression in cells infected with LV3-shMARCH7-1 or LV3-shMARCH7-2 was significantly lower than in control cells. LV3-shMARCH7-1, lentiviral vector expressing shRNA targeting MARCH7; LV, lentiviral vector.

    Journal: Oncology Letters

    Article Title: Ubiquitin E3 Ligase MARCH7 promotes proliferation and invasion of cervical cancer cells through VAV2-RAC1-CDC42 pathway

    doi: 10.3892/ol.2018.8908

    Figure Lengend Snippet: MARCH7 regulated VAV2-RAC1-CDC42 pathway. (A) The interaction between MARCH7 and VAV2 was identified by Co-IP assay. Cells were co-transfected with Flag-VAV2 and HA-MARCH7, and control group was established simultaneously, cells were then harvested 24 h later. Anti-HA antibodies were used to pull the interaction protein. Then, they were detected by anti-Flag antibodies. Results showed that Flag bands could not be detected in the cells transfected with Flag-VAV2 (lane 1) or HA-MARCH7 (lane 3) only. However, it can be detected in cells co-transfected with both Flag-VAV2 and HA-MARCH7 (lane 2), which indicated that there existed interaction between MARCH7 and VAV2 in vivo . (B) The expression of VAV2, RAC1 and CDC42 were determined by western blot analysis. The VAV2, RAC1 and CDC42 expression in cells infected with LV3-shMARCH7-1 or LV3-shMARCH7-2 was significantly lower than in control cells. LV3-shMARCH7-1, lentiviral vector expressing shRNA targeting MARCH7; LV, lentiviral vector.

    Article Snippet: Lentiviral vector expressing shRNA targeting MARCH7 (named LV3-shMarch7-1 and LV3-shMarch7-2) were provided from Genepharma Co., Ltd..

    Techniques: Co-Immunoprecipitation Assay, Transfection, In Vivo, Expressing, Western Blot, Infection, Plasmid Preparation, shRNA