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Shanghai Genechem control vector expressing gfp
Expression of <t>GFP</t> and Foxc2 genes after <t>transfection.</t> (A) Transfection efficiency at various MOIs (10, 50, 100 and 200) 72 hours after transfection (×100). (B) Cell proliferation after transfection in the Lv-Foxc2 and Lv-GFP groups detected using MTT assay. (C) Foxc2 gene expression measured by real-time PCR 72 hours after transfection. GAPDH was used as a housekeeping gene. (D) Foxc2 protein expression evaluated by western blot 72 hours after transfection. Results were expressed as mean±SD of triplicate experiments. #p
Control Vector Expressing Gfp, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
control vector expressing gfp - by Bioz Stars, 2020-08
85/100 stars

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Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells

Journal: BMC Musculoskeletal Disorders

doi: 10.1186/1471-2474-14-199

Expression of GFP and Foxc2 genes after transfection. (A) Transfection efficiency at various MOIs (10, 50, 100 and 200) 72 hours after transfection (×100). (B) Cell proliferation after transfection in the Lv-Foxc2 and Lv-GFP groups detected using MTT assay. (C) Foxc2 gene expression measured by real-time PCR 72 hours after transfection. GAPDH was used as a housekeeping gene. (D) Foxc2 protein expression evaluated by western blot 72 hours after transfection. Results were expressed as mean±SD of triplicate experiments. #p
Figure Legend Snippet: Expression of GFP and Foxc2 genes after transfection. (A) Transfection efficiency at various MOIs (10, 50, 100 and 200) 72 hours after transfection (×100). (B) Cell proliferation after transfection in the Lv-Foxc2 and Lv-GFP groups detected using MTT assay. (C) Foxc2 gene expression measured by real-time PCR 72 hours after transfection. GAPDH was used as a housekeeping gene. (D) Foxc2 protein expression evaluated by western blot 72 hours after transfection. Results were expressed as mean±SD of triplicate experiments. #p

Techniques Used: Expressing, Transfection, MTT Assay, Real-time Polymerase Chain Reaction, Western Blot

The effect of Foxc2 on osteogenic differentiation. (A , B and C) BMSCs were transfected with Lv-GFP or Lv-Foxc2 for 2 weeks. Cells stained with Alizarin red S ( A and B , a1 presents the Lv-Foxc2 group, and a2 presents the Lv-GFP group). Cells stained with ALP ( C , c1 presents the Lv-Foxc2 group and c2 presents the Lv-GFP group). (D) ALP activities in BMSCs. (E) Expressions of CON and Runx2 mRNA detected by real-time PCR 72 hours after transfection. The relative density of each gene was statistically analyzed. #p
Figure Legend Snippet: The effect of Foxc2 on osteogenic differentiation. (A , B and C) BMSCs were transfected with Lv-GFP or Lv-Foxc2 for 2 weeks. Cells stained with Alizarin red S ( A and B , a1 presents the Lv-Foxc2 group, and a2 presents the Lv-GFP group). Cells stained with ALP ( C , c1 presents the Lv-Foxc2 group and c2 presents the Lv-GFP group). (D) ALP activities in BMSCs. (E) Expressions of CON and Runx2 mRNA detected by real-time PCR 72 hours after transfection. The relative density of each gene was statistically analyzed. #p

Techniques Used: Transfection, Staining, ALP Assay, Real-time Polymerase Chain Reaction

Foxc2 overexpression induces activation of ERK and PI3K in BMSCs. (A , B) BMSCs were transfected with Lv-GFP, Lv-Foxc2 or Lv-Foxc2 following pretreatment with 50 μM PD98059 or 10 μM LY294002 for 1 h. Cell lysates harvested after 2 weeks of culture were analyzed by SDS-PAGE and Western blot with anti ERK or anti-PI3K antibody. The result of ERK or PI3K expression was indicated by the ratio of band intensity of ERK or PI3K with β-actin. *p
Figure Legend Snippet: Foxc2 overexpression induces activation of ERK and PI3K in BMSCs. (A , B) BMSCs were transfected with Lv-GFP, Lv-Foxc2 or Lv-Foxc2 following pretreatment with 50 μM PD98059 or 10 μM LY294002 for 1 h. Cell lysates harvested after 2 weeks of culture were analyzed by SDS-PAGE and Western blot with anti ERK or anti-PI3K antibody. The result of ERK or PI3K expression was indicated by the ratio of band intensity of ERK or PI3K with β-actin. *p

Techniques Used: Over Expression, Activation Assay, Transfection, SDS Page, Western Blot, Expressing

Related Articles

Transfection:

Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
Article Snippet: .. Lentiviral production and transfection A lentiviral vector expressing human Foxc2 and a control vector expressing GFP were purchased from Shanghai GeneChem Co., China. ..

Plasmid Preparation:

Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
Article Snippet: .. Lentiviral production and transfection A lentiviral vector expressing human Foxc2 and a control vector expressing GFP were purchased from Shanghai GeneChem Co., China. ..

Expressing:

Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells
Article Snippet: .. Lentiviral production and transfection A lentiviral vector expressing human Foxc2 and a control vector expressing GFP were purchased from Shanghai GeneChem Co., China. ..

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    Shanghai Genechem control vector expressing gfp
    Expression of <t>GFP</t> and Foxc2 genes after <t>transfection.</t> (A) Transfection efficiency at various MOIs (10, 50, 100 and 200) 72 hours after transfection (×100). (B) Cell proliferation after transfection in the Lv-Foxc2 and Lv-GFP groups detected using MTT assay. (C) Foxc2 gene expression measured by real-time PCR 72 hours after transfection. GAPDH was used as a housekeeping gene. (D) Foxc2 protein expression evaluated by western blot 72 hours after transfection. Results were expressed as mean±SD of triplicate experiments. #p
    Control Vector Expressing Gfp, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control vector expressing gfp/product/Shanghai Genechem
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control vector expressing gfp - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Expression of GFP and Foxc2 genes after transfection. (A) Transfection efficiency at various MOIs (10, 50, 100 and 200) 72 hours after transfection (×100). (B) Cell proliferation after transfection in the Lv-Foxc2 and Lv-GFP groups detected using MTT assay. (C) Foxc2 gene expression measured by real-time PCR 72 hours after transfection. GAPDH was used as a housekeeping gene. (D) Foxc2 protein expression evaluated by western blot 72 hours after transfection. Results were expressed as mean±SD of triplicate experiments. #p

    Journal: BMC Musculoskeletal Disorders

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells

    doi: 10.1186/1471-2474-14-199

    Figure Lengend Snippet: Expression of GFP and Foxc2 genes after transfection. (A) Transfection efficiency at various MOIs (10, 50, 100 and 200) 72 hours after transfection (×100). (B) Cell proliferation after transfection in the Lv-Foxc2 and Lv-GFP groups detected using MTT assay. (C) Foxc2 gene expression measured by real-time PCR 72 hours after transfection. GAPDH was used as a housekeeping gene. (D) Foxc2 protein expression evaluated by western blot 72 hours after transfection. Results were expressed as mean±SD of triplicate experiments. #p

    Article Snippet: Lentiviral production and transfection A lentiviral vector expressing human Foxc2 and a control vector expressing GFP were purchased from Shanghai GeneChem Co., China.

    Techniques: Expressing, Transfection, MTT Assay, Real-time Polymerase Chain Reaction, Western Blot

    The effect of Foxc2 on osteogenic differentiation. (A , B and C) BMSCs were transfected with Lv-GFP or Lv-Foxc2 for 2 weeks. Cells stained with Alizarin red S ( A and B , a1 presents the Lv-Foxc2 group, and a2 presents the Lv-GFP group). Cells stained with ALP ( C , c1 presents the Lv-Foxc2 group and c2 presents the Lv-GFP group). (D) ALP activities in BMSCs. (E) Expressions of CON and Runx2 mRNA detected by real-time PCR 72 hours after transfection. The relative density of each gene was statistically analyzed. #p

    Journal: BMC Musculoskeletal Disorders

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells

    doi: 10.1186/1471-2474-14-199

    Figure Lengend Snippet: The effect of Foxc2 on osteogenic differentiation. (A , B and C) BMSCs were transfected with Lv-GFP or Lv-Foxc2 for 2 weeks. Cells stained with Alizarin red S ( A and B , a1 presents the Lv-Foxc2 group, and a2 presents the Lv-GFP group). Cells stained with ALP ( C , c1 presents the Lv-Foxc2 group and c2 presents the Lv-GFP group). (D) ALP activities in BMSCs. (E) Expressions of CON and Runx2 mRNA detected by real-time PCR 72 hours after transfection. The relative density of each gene was statistically analyzed. #p

    Article Snippet: Lentiviral production and transfection A lentiviral vector expressing human Foxc2 and a control vector expressing GFP were purchased from Shanghai GeneChem Co., China.

    Techniques: Transfection, Staining, ALP Assay, Real-time Polymerase Chain Reaction

    Foxc2 overexpression induces activation of ERK and PI3K in BMSCs. (A , B) BMSCs were transfected with Lv-GFP, Lv-Foxc2 or Lv-Foxc2 following pretreatment with 50 μM PD98059 or 10 μM LY294002 for 1 h. Cell lysates harvested after 2 weeks of culture were analyzed by SDS-PAGE and Western blot with anti ERK or anti-PI3K antibody. The result of ERK or PI3K expression was indicated by the ratio of band intensity of ERK or PI3K with β-actin. *p

    Journal: BMC Musculoskeletal Disorders

    Article Title: Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells

    doi: 10.1186/1471-2474-14-199

    Figure Lengend Snippet: Foxc2 overexpression induces activation of ERK and PI3K in BMSCs. (A , B) BMSCs were transfected with Lv-GFP, Lv-Foxc2 or Lv-Foxc2 following pretreatment with 50 μM PD98059 or 10 μM LY294002 for 1 h. Cell lysates harvested after 2 weeks of culture were analyzed by SDS-PAGE and Western blot with anti ERK or anti-PI3K antibody. The result of ERK or PI3K expression was indicated by the ratio of band intensity of ERK or PI3K with β-actin. *p

    Article Snippet: Lentiviral production and transfection A lentiviral vector expressing human Foxc2 and a control vector expressing GFP were purchased from Shanghai GeneChem Co., China.

    Techniques: Over Expression, Activation Assay, Transfection, SDS Page, Western Blot, Expressing