Journal: Oncology Letters

Article Title: Identification of feature genes and molecular mechanisms involved in cell communication in uveal melanoma through analysis of single‑cell sequencing data

doi: 10.3892/ol.2024.14636

Figure Lengend Snippet: Small interfering RNA sequences used in the present study.

Article Snippet: For gene knockdown, small interfering (si)RNA duplexes targeting human genes [SEC14-like lipid binding 1 ( SEC14L1 ), plexin D1 ( PLXND1 ), tropomyosin 4 ( TPM4 ) or GNAI2 ] and a control siRNA were designed and synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: Small Interfering RNA, Sequencing, Control

Journal: Oncology Letters

Article Title: Identification of feature genes and molecular mechanisms involved in cell communication in uveal melanoma through analysis of single‑cell sequencing data

doi: 10.3892/ol.2024.14636

Figure Lengend Snippet: In vitro biofunction of PLXND1 and GNAI2 in C918 cells. Viability of C918 cells after (A) GNAI2 and (B) PLXND1 siRNA transfection. Gap closure rate of the (C) siGNAI2 and (D) siPLX1 groups after 20 h. (E) Representative images of the gap closure assay; scale bar, 200 µm. **P<0.01, ***P<0.001, ****P<0.0001. CON, control; GNAI2, G protein subunit α I2; PLXND1, plexin D1; si, small interfering (RNA).

Article Snippet: For gene knockdown, small interfering (si)RNA duplexes targeting human genes [SEC14-like lipid binding 1 ( SEC14L1 ), plexin D1 ( PLXND1 ), tropomyosin 4 ( TPM4 ) or GNAI2 ] and a control siRNA were designed and synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: In Vitro, Transfection, Control, Small Interfering RNA

Journal: Discover Oncology

Article Title: Hsa_circ_ROBO1 competes with miR-324-3p and upregulates NME1 to promote tumor metastasis in nasopharyngeal carcinoma

doi: 10.1007/s12672-024-01220-9

Figure Lengend Snippet: Overexpression of NME1 reversed the circCRIM1 shRNA-induced inhibition of NPC cell invasion, proliferation and migration. A . the circ_ROBO1, miR-324-3p and NME1 levels in NPC cells were determined by RT–qPCR. B . The NME1 levels in NPC cells were determined by Western blot. GAPDH was used for normalization. C . NPC cell viability was assessed by the MTT assay. D . NPC cell invasion and migration were assessed by the transwell assay. **P < 0.01. All data are expressed as the mean ± standard deviation (SD)

Article Snippet: circ_ROBO1 small interfering RNA (si-circ_ROBO1#1/#2), circ_ROBO1 overexpressed plasmid, NME1 siRNA (si-NME1) or siRNA negative control (si-NC) at 100 nM, miR-324-3p mimics/inhibitors, or corresponding negative controls at 50 nM (GenePharma) were transfected into HONE1 and HK1 cells using Lipofectamine 3000 (Invitrogen).

Techniques: Over Expression, shRNA, Inhibition, Migration, Quantitative RT-PCR, Western Blot, MTT Assay, Transwell Assay, Standard Deviation