control collagenase inhibitor z pro d leu d ala nhoh  (Millipore)

 
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    Name:
    Collagenase I
    Description:
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including MSDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    Catalog Number:
    1148089
    Price:
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    Structured Review

    Millipore control collagenase inhibitor z pro d leu d ala nhoh
    Collagenase I
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including MSDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    https://www.bioz.com/result/control collagenase inhibitor z pro d leu d ala nhoh/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control collagenase inhibitor z pro d leu d ala nhoh - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "Neutrophils Increase or Reduce Parasite Burden in Trypanosoma cruzi-Infected Macrophages, Depending on Host Strain: Role of Neutrophil Elastase"

    Article Title: Neutrophils Increase or Reduce Parasite Burden in Trypanosoma cruzi-Infected Macrophages, Depending on Host Strain: Role of Neutrophil Elastase

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0090582

    Neutrophil elastase potentiates the microbicidal response of T. cruzi in vitro . (A) Parasites released in infected C57BL/6 macrophages cultures alone or in presence of solvent (DMSO), control inhibitor (collagenase inhibitor Z-Pro-D-Leu-AAPV-D-Ala-NHOH; used at 10 µg/ml) or NE inhibitor (MeOSuc-AAPV-cmk; used at 10 µg/ml). (B) Neutrophil elastase (100 ng/ml) was added to infected cultured macrophages in the presence or absence of selective iNOS inhibitor (L-NIL). The number of trypomastigotes was counted in a Neubauer chamber after 7 days of culture. Production of TNF-α and NO released into the supernatant by T. cruzi infected macrophages after 24 h incubation in medium alone or in the presence of neutrophil elastase, at 100 ng/ml. Measurements were performed in triplicates of three different experiments. Statistical significance was determined by ANOVA (A) or t test (B).
    Figure Legend Snippet: Neutrophil elastase potentiates the microbicidal response of T. cruzi in vitro . (A) Parasites released in infected C57BL/6 macrophages cultures alone or in presence of solvent (DMSO), control inhibitor (collagenase inhibitor Z-Pro-D-Leu-AAPV-D-Ala-NHOH; used at 10 µg/ml) or NE inhibitor (MeOSuc-AAPV-cmk; used at 10 µg/ml). (B) Neutrophil elastase (100 ng/ml) was added to infected cultured macrophages in the presence or absence of selective iNOS inhibitor (L-NIL). The number of trypomastigotes was counted in a Neubauer chamber after 7 days of culture. Production of TNF-α and NO released into the supernatant by T. cruzi infected macrophages after 24 h incubation in medium alone or in the presence of neutrophil elastase, at 100 ng/ml. Measurements were performed in triplicates of three different experiments. Statistical significance was determined by ANOVA (A) or t test (B).

    Techniques Used: In Vitro, Infection, Cell Culture, Incubation

    Related Articles

    Incubation:

    Article Title: Accelerating skin wound healing by M-CSF through generating SSEA-1 and -3 stem cells in the injured sites
    Article Snippet: Isolation and culture of cells from mouse wounded skin or normal skin Four punch biopsies (6 mm) from wounded skin and eight punch biopsies from normal skin for each sacrificed mouse were taken. .. Tissue was minced into small pieces and incubated with type-1 collagenase (1 mg/ml in PBS, sigma) for 90 minutes in a shaker at 225 rpm and 37 °C. ..

    Article Title: CD105 (Endoglin)-Negative Murine Mesenchymal Stromal Cells Define a New Multipotent Subpopulation with Distinct Differentiation and Immunomodulatory Capacities
    Article Snippet: Cells were subsequently washed with PBS, fixed with 3.7% paraformaldehyde (15 minutes at room temperature) and stained with 1% cresyl violet. .. Flow cytometry Collagenase type I digests or cultured ASCs were incubated with 7AAD (Sigma-Aldrich) and 2.4G2 (eBioscience) and then stained with Abs specific for CD11b, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD61, CD73, CD105, CD140a, CD146, sca-1, MHC class I and MHC class II (all from eBioscience) or anti-TGF-βRI (ALK5) and anti-TGF-βRII Abs (R & D Systems, Minneapolis, MN). .. Cells were acquired and analyzed on a BD FACS Canto II using the FACS Diva software (BD Biosciences, Bedford, MA).

    Multiple Displacement Amplification:

    Article Title: Myeloid-Derived Lymphatic Endothelial Cell Progenitors Significantly Contribute to Lymphatic Metastasis in Clinical Breast Cancer
    Article Snippet: .. Orthotopic MDA-MB-231-Luc tumors harvested at a volume of 500 mm3 were digested by collagenase type III (225 U/mL) and hyaluronidase (100 U/mL), both from Sigma-Aldrich. .. Tumor-associated CD11b+ cells were isolated using anti-CD11b IgG-conjugated magnetic beads (Miltenyi Biotec).

    Concentration Assay:

    Article Title: MMP-Sensitive PEG Diacrylate Hydrogels with Spatial Variations in Matrix Properties Stimulate Directional Vascular Sprout Formation
    Article Snippet: PBFP gradient hydrogels and their bulk equivalents were sectioned as outlined above and swollen in deionized water for 36 hours before being transferred to pre-weighed tubes and incubated in 10 mM HEPES buffered saline (HBS; 10 mM HEPES sodium salt and 137 mM NaCl) with 1 mM CaCl2 (pH 7.4) overnight. .. Collagenase-1A (Sigma Aldrich) at a concentration of 1 µg/mL was added to the hydrogel sections and the swollen weight of each section was recorded every two hours until complete hydrogel degradation was achieved. ..

    Mouse Assay:

    Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice
    Article Snippet: .. In this study, we used collagenase-induced ICH mice model to investigate whether A68930 (DRD1 specific agonist, Sigma) can improve neurological outcome through inhibition of NLRP3-mediated inflammation. .. AnimalsA total of 106 (excluding 3 mice that died because of anesthetic overdose) male CD1 mice (Charles River, Wilmington, MA, USA) weighing 30 to 34 g were used in these experiments.

    Inhibition:

    Article Title: Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice
    Article Snippet: .. In this study, we used collagenase-induced ICH mice model to investigate whether A68930 (DRD1 specific agonist, Sigma) can improve neurological outcome through inhibition of NLRP3-mediated inflammation. .. AnimalsA total of 106 (excluding 3 mice that died because of anesthetic overdose) male CD1 mice (Charles River, Wilmington, MA, USA) weighing 30 to 34 g were used in these experiments.

    Flow Cytometry:

    Article Title: CD105 (Endoglin)-Negative Murine Mesenchymal Stromal Cells Define a New Multipotent Subpopulation with Distinct Differentiation and Immunomodulatory Capacities
    Article Snippet: Cells were subsequently washed with PBS, fixed with 3.7% paraformaldehyde (15 minutes at room temperature) and stained with 1% cresyl violet. .. Flow cytometry Collagenase type I digests or cultured ASCs were incubated with 7AAD (Sigma-Aldrich) and 2.4G2 (eBioscience) and then stained with Abs specific for CD11b, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD61, CD73, CD105, CD140a, CD146, sca-1, MHC class I and MHC class II (all from eBioscience) or anti-TGF-βRI (ALK5) and anti-TGF-βRII Abs (R & D Systems, Minneapolis, MN). .. Cells were acquired and analyzed on a BD FACS Canto II using the FACS Diva software (BD Biosciences, Bedford, MA).

    Cytometry:

    Article Title: CD105 (Endoglin)-Negative Murine Mesenchymal Stromal Cells Define a New Multipotent Subpopulation with Distinct Differentiation and Immunomodulatory Capacities
    Article Snippet: Cells were subsequently washed with PBS, fixed with 3.7% paraformaldehyde (15 minutes at room temperature) and stained with 1% cresyl violet. .. Flow cytometry Collagenase type I digests or cultured ASCs were incubated with 7AAD (Sigma-Aldrich) and 2.4G2 (eBioscience) and then stained with Abs specific for CD11b, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD61, CD73, CD105, CD140a, CD146, sca-1, MHC class I and MHC class II (all from eBioscience) or anti-TGF-βRI (ALK5) and anti-TGF-βRII Abs (R & D Systems, Minneapolis, MN). .. Cells were acquired and analyzed on a BD FACS Canto II using the FACS Diva software (BD Biosciences, Bedford, MA).

    Cell Culture:

    Article Title: CD105 (Endoglin)-Negative Murine Mesenchymal Stromal Cells Define a New Multipotent Subpopulation with Distinct Differentiation and Immunomodulatory Capacities
    Article Snippet: Cells were subsequently washed with PBS, fixed with 3.7% paraformaldehyde (15 minutes at room temperature) and stained with 1% cresyl violet. .. Flow cytometry Collagenase type I digests or cultured ASCs were incubated with 7AAD (Sigma-Aldrich) and 2.4G2 (eBioscience) and then stained with Abs specific for CD11b, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD61, CD73, CD105, CD140a, CD146, sca-1, MHC class I and MHC class II (all from eBioscience) or anti-TGF-βRI (ALK5) and anti-TGF-βRII Abs (R & D Systems, Minneapolis, MN). .. Cells were acquired and analyzed on a BD FACS Canto II using the FACS Diva software (BD Biosciences, Bedford, MA).

    Staining:

    Article Title: CD105 (Endoglin)-Negative Murine Mesenchymal Stromal Cells Define a New Multipotent Subpopulation with Distinct Differentiation and Immunomodulatory Capacities
    Article Snippet: Cells were subsequently washed with PBS, fixed with 3.7% paraformaldehyde (15 minutes at room temperature) and stained with 1% cresyl violet. .. Flow cytometry Collagenase type I digests or cultured ASCs were incubated with 7AAD (Sigma-Aldrich) and 2.4G2 (eBioscience) and then stained with Abs specific for CD11b, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD61, CD73, CD105, CD140a, CD146, sca-1, MHC class I and MHC class II (all from eBioscience) or anti-TGF-βRI (ALK5) and anti-TGF-βRII Abs (R & D Systems, Minneapolis, MN). .. Cells were acquired and analyzed on a BD FACS Canto II using the FACS Diva software (BD Biosciences, Bedford, MA).

    Isolation:

    Article Title: Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro
    Article Snippet: Subsequently, the pulp chamber was gently rinsed with phosphate-buffered saline (PBS; GE Healthcare Life Sciences HyClone Laboratories, Malborough, MA, USA) containing 100 IU/mL penicillin and 100 µg/mL streptomycin solutions (Gibco, ThermoFisher Scientific, Waltham, MA, USA) to wash out the remaining pulp tissue. .. Following the mechanical disruption, the isolated pulp tissue was subjected to further enzymatic digestion using a mixture of collagenase I (3 mg/mL, Sigma-Aldrich, St. Louis, MO, USA) and dispase (4 mg/mL, Sigma-Aldrich) for 30 min at 37 °C. .. The enzymes were inactivated by adding a complete cell culture medium (DMEM/F12 supplemented with 10% FBS, Sigma-Aldrich; and 100 IU/mL penicillin, 100 µg/mL streptomycin, Gibco, ThermoFisher Scientific).

    Injection:

    Article Title: Overexpression of mechanical sensitive miR-337-3p alleviates ectopic ossification in rat tendinopathy model via targeting IRS1 and Nox4 of tendon-derived stem cells
    Article Snippet: .. Bacterial collagenase I (20 μl of 0.015 mg/ml in saline; Sigma-Aldrich) or saline was injected into the patellar tendon intratendinously with a 29-gauge needle in one limb. .. Three days later, 50 μl rno-miR-337-3p-overexpressing lentivirus (1 × 109 TU/ml) or negative control lentivirus (GenePharma) was injected into the patellar tendon where collagenase I treated before.

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  • 97
    Millipore control collagenase inhibitor z pro d leu d ala nhoh
    Neutrophil elastase potentiates the microbicidal response of T. cruzi in vitro . (A) Parasites released in infected C57BL/6 macrophages cultures alone or in presence of solvent (DMSO), control inhibitor (collagenase inhibitor <t>Z-Pro-D-Leu-AAPV-D-Ala-NHOH;</t> used at 10 µg/ml) or NE inhibitor (MeOSuc-AAPV-cmk; used at 10 µg/ml). (B) Neutrophil elastase (100 ng/ml) was added to infected cultured macrophages in the presence or absence of selective iNOS inhibitor (L-NIL). The number of trypomastigotes was counted in a Neubauer chamber after 7 days of culture. Production of TNF-α and NO released into the supernatant by T. cruzi infected macrophages after 24 h incubation in medium alone or in the presence of neutrophil elastase, at 100 ng/ml. Measurements were performed in triplicates of three different experiments. Statistical significance was determined by ANOVA (A) or t test (B).
    Control Collagenase Inhibitor Z Pro D Leu D Ala Nhoh, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control collagenase inhibitor z pro d leu d ala nhoh/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control collagenase inhibitor z pro d leu d ala nhoh - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    Neutrophil elastase potentiates the microbicidal response of T. cruzi in vitro . (A) Parasites released in infected C57BL/6 macrophages cultures alone or in presence of solvent (DMSO), control inhibitor (collagenase inhibitor Z-Pro-D-Leu-AAPV-D-Ala-NHOH; used at 10 µg/ml) or NE inhibitor (MeOSuc-AAPV-cmk; used at 10 µg/ml). (B) Neutrophil elastase (100 ng/ml) was added to infected cultured macrophages in the presence or absence of selective iNOS inhibitor (L-NIL). The number of trypomastigotes was counted in a Neubauer chamber after 7 days of culture. Production of TNF-α and NO released into the supernatant by T. cruzi infected macrophages after 24 h incubation in medium alone or in the presence of neutrophil elastase, at 100 ng/ml. Measurements were performed in triplicates of three different experiments. Statistical significance was determined by ANOVA (A) or t test (B).

    Journal: PLoS ONE

    Article Title: Neutrophils Increase or Reduce Parasite Burden in Trypanosoma cruzi-Infected Macrophages, Depending on Host Strain: Role of Neutrophil Elastase

    doi: 10.1371/journal.pone.0090582

    Figure Lengend Snippet: Neutrophil elastase potentiates the microbicidal response of T. cruzi in vitro . (A) Parasites released in infected C57BL/6 macrophages cultures alone or in presence of solvent (DMSO), control inhibitor (collagenase inhibitor Z-Pro-D-Leu-AAPV-D-Ala-NHOH; used at 10 µg/ml) or NE inhibitor (MeOSuc-AAPV-cmk; used at 10 µg/ml). (B) Neutrophil elastase (100 ng/ml) was added to infected cultured macrophages in the presence or absence of selective iNOS inhibitor (L-NIL). The number of trypomastigotes was counted in a Neubauer chamber after 7 days of culture. Production of TNF-α and NO released into the supernatant by T. cruzi infected macrophages after 24 h incubation in medium alone or in the presence of neutrophil elastase, at 100 ng/ml. Measurements were performed in triplicates of three different experiments. Statistical significance was determined by ANOVA (A) or t test (B).

    Article Snippet: Monolayers were also treated with the specific NE inhibitor methoxysuccinyl-Ala-Ala-Pro-Val-chloromethylketone (MeoSuc-AAPV-CMK; Calbiochen-Novabiochem, La Jolla, CA), control collagenase inhibitor Z-Pro-D-Leu-D-Ala-NHOH (Calbiochem-Novabiochem), both at 10 µg/ml, or equivalent dosage of solvent (DMSO) alone.

    Techniques: In Vitro, Infection, Cell Culture, Incubation