connexin 43 cx43 polyclonal rabbit antibodies  (Santa Cruz Biotechnology)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    connexin 43
    Description:
    supplied as 0 5 mg 0 1 ml substrate suitable for kinase assays Suitable substrate for ERK 2 sc 4806 Store at 20° C Recognition Motif X X S T P
    Catalog Number:
    SC-24541
    Price:
    None
    Category:
    Chemicals Other Chemicals Enzyme Substrates connexin 43 mSer 262
    Buy from Supplier


    Structured Review

    Santa Cruz Biotechnology connexin 43 cx43 polyclonal rabbit antibodies
    supplied as 0 5 mg 0 1 ml substrate suitable for kinase assays Suitable substrate for ERK 2 sc 4806 Store at 20° C Recognition Motif X X S T P
    https://www.bioz.com/result/connexin 43 cx43 polyclonal rabbit antibodies/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    connexin 43 cx43 polyclonal rabbit antibodies - by Bioz Stars, 2021-05
    93/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Activin A Modulates CRIPTO-1/HNF4α+ Cells to Guide Cardiac Differentiation from Human Embryonic Stem Cells
    Article Snippet: Cells were fixed with 4% paraformaldehyde (PFA; Polysciences) for 10 minutes at 4°C, permeabilized in PBS (Thermo Fisher Scientific) containing 0.2% Triton X-100 (Sigma-Aldrich) and 1% bovine serum albumin (BSA; Sigma-Aldrich) for 30 minutes, and blocked in 10% donkey serum (Sigma-Aldrich) for 30 minutes. .. Cells were incubated overnight at 4°C with the following primary antibodies: cardiac myosin heavy chain (cMyHC; Abcam BA-G5; 10 μ g/mL), connexin 43 (CX43; Santa Cruz H-150; 0.67 μ g/mL), and hepatocyte nuclear factor 4 alpha (HNF4α ; Abcam, K9218; 5 μ g/mL), followed by the appropriate Alexa Fluor 488- and 594-conjugated secondary antibody (Thermo Fisher Scientific; 4 μ g/mL). .. Nuclei were stained with Hoechst (33342, Thermo Fisher Scientific; 1 : 3000) for 1 minute.

    Purification:

    Article Title: Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayers *
    Article Snippet: After lysis, membranes were separated from the crude cell extract by centrifugation and solubilized in DDM, which is known to be an efficient detergent for connexon isolation from membrane fragments ( ). .. Solubilized Cx43 and Cx43-GFP were then purified by affinity chromatography using a Ni-NTA-agarose resin. .. Assessments for purity were based on Coomassie-stained denaturing gels in which the bands were identified as Cx43 and Cx43-GFP using Western blot analysis ( ).

    Affinity Chromatography:

    Article Title: Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayers *
    Article Snippet: After lysis, membranes were separated from the crude cell extract by centrifugation and solubilized in DDM, which is known to be an efficient detergent for connexon isolation from membrane fragments ( ). .. Solubilized Cx43 and Cx43-GFP were then purified by affinity chromatography using a Ni-NTA-agarose resin. .. Assessments for purity were based on Coomassie-stained denaturing gels in which the bands were identified as Cx43 and Cx43-GFP using Western blot analysis ( ).

    other:

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1
    Article Snippet: Consistent with the finding in young OVX and OVX+E2 rats, the mRNA levels of Cx43 in 6M, 18M and 18M+E2 are comparative which suggested a post-transcriptional regulation of Cx43 protein level (Fig G).

    Article Title: 17Beta-Estradiol Inhibits Calcium-Activated Potassium Channel Expressions in Rat Whole Bladder
    Article Snippet: Levels of IK, SK2, Cx26, and Cx43 did not change significantly under any condition ( , , , ).

    Article Title: Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells
    Article Snippet: We also observed that melatonin suppressed the phosphorylation of Cx43 in H2 O2 -treated HaCaT cells ( ).

    Article Title: Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayers *
    Article Snippet: Under the given conditions, Cx43 exhibited a main conductance of 224 ± 26 pS, similar to the fully open state conductance, G = 220 ± 11 pS, of Cx43 hemichannels expressed in HeLa cells ( ) and approximately double that of Cx43 gap junctions ( , ).

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1
    Article Snippet: Interestingly, the mRNA level of Cx43 showed no significant differences either between 6M and 18M rats or among rats of sham, OVX and OVX+E2 (Fig. F).

    End Labeling:

    Article Title: Mitochondrial targeted peptides preserve mitochondrial organization and decrease reversible myocardial changes in early swine metabolic syndrome
    Article Snippet: Intramyocardial fat deposition was measured by Oil-red-O staining (Abcam; Cat#ab150678), and oxidative stress by in situ production of superoxide anion detected by dihydroethidium (DHE: 20 μM/l; Sigma), oxidized low-density lipoprotein (Ox-LDL) staining (1:50; Abcam), myocardial protein expression of the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1; Cat# ab60178; Abcam), and the NADPH oxidase subunits gp91(Millipore; Cat# 07-024) and p67 (Cell Signaling; Cat# sc-15342). .. Myocardial apoptosis was assessed in LV sections double-stained with terminal deoxynucleotidyl transferase-mediated dUTP nick end end-labeling (TUNEL; Promega, Madison, WI, USA) and connexin-43 fluorescent staining, and caspase-3 (both 1:200; Santa Cruz Biotechnology, Inc.) fluorescent staining. .. The number of TUNEL+ and caspase-3+ cells was quantified and averaged in each group.

    TUNEL Assay:

    Article Title: Mitochondrial targeted peptides preserve mitochondrial organization and decrease reversible myocardial changes in early swine metabolic syndrome
    Article Snippet: Intramyocardial fat deposition was measured by Oil-red-O staining (Abcam; Cat#ab150678), and oxidative stress by in situ production of superoxide anion detected by dihydroethidium (DHE: 20 μM/l; Sigma), oxidized low-density lipoprotein (Ox-LDL) staining (1:50; Abcam), myocardial protein expression of the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1; Cat# ab60178; Abcam), and the NADPH oxidase subunits gp91(Millipore; Cat# 07-024) and p67 (Cell Signaling; Cat# sc-15342). .. Myocardial apoptosis was assessed in LV sections double-stained with terminal deoxynucleotidyl transferase-mediated dUTP nick end end-labeling (TUNEL; Promega, Madison, WI, USA) and connexin-43 fluorescent staining, and caspase-3 (both 1:200; Santa Cruz Biotechnology, Inc.) fluorescent staining. .. The number of TUNEL+ and caspase-3+ cells was quantified and averaged in each group.

    Staining:

    Article Title: Mitochondrial targeted peptides preserve mitochondrial organization and decrease reversible myocardial changes in early swine metabolic syndrome
    Article Snippet: Intramyocardial fat deposition was measured by Oil-red-O staining (Abcam; Cat#ab150678), and oxidative stress by in situ production of superoxide anion detected by dihydroethidium (DHE: 20 μM/l; Sigma), oxidized low-density lipoprotein (Ox-LDL) staining (1:50; Abcam), myocardial protein expression of the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1; Cat# ab60178; Abcam), and the NADPH oxidase subunits gp91(Millipore; Cat# 07-024) and p67 (Cell Signaling; Cat# sc-15342). .. Myocardial apoptosis was assessed in LV sections double-stained with terminal deoxynucleotidyl transferase-mediated dUTP nick end end-labeling (TUNEL; Promega, Madison, WI, USA) and connexin-43 fluorescent staining, and caspase-3 (both 1:200; Santa Cruz Biotechnology, Inc.) fluorescent staining. .. The number of TUNEL+ and caspase-3+ cells was quantified and averaged in each group.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Santa Cruz Biotechnology cx 43
    Representative images obtained with immunofluorescence to Cx 32, Cx 36 and <t>Cx</t> 43 in CA1 and CA3 of the right and left hippocampus (RH and LH, respectively) as well as in the dentate gyrus (DG) in the control (NaCl, 0.9 %) and experimental groups (4-AP, 10 nmol). Calibration bar, 50 μm. The right graphs represent the mean ± SEM of the cellular density for Cx 32-, Cx 36-, Cx 43-labelled cells in the three analyzed regions: CA1 and CA3 of the RH and LH, and the DG. Significant differences between the control and experimental groups were assessed with a Student’s t -test
    Cx 43, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cx 43/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cx 43 - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    Representative images obtained with immunofluorescence to Cx 32, Cx 36 and Cx 43 in CA1 and CA3 of the right and left hippocampus (RH and LH, respectively) as well as in the dentate gyrus (DG) in the control (NaCl, 0.9 %) and experimental groups (4-AP, 10 nmol). Calibration bar, 50 μm. The right graphs represent the mean ± SEM of the cellular density for Cx 32-, Cx 36-, Cx 43-labelled cells in the three analyzed regions: CA1 and CA3 of the RH and LH, and the DG. Significant differences between the control and experimental groups were assessed with a Student’s t -test

    Journal: Journal of Biomedical Science

    Article Title: Analysis of connexin expression during seizures induced by 4-aminopyridine in the rat hippocampus

    doi: 10.1186/s12929-015-0176-5

    Figure Lengend Snippet: Representative images obtained with immunofluorescence to Cx 32, Cx 36 and Cx 43 in CA1 and CA3 of the right and left hippocampus (RH and LH, respectively) as well as in the dentate gyrus (DG) in the control (NaCl, 0.9 %) and experimental groups (4-AP, 10 nmol). Calibration bar, 50 μm. The right graphs represent the mean ± SEM of the cellular density for Cx 32-, Cx 36-, Cx 43-labelled cells in the three analyzed regions: CA1 and CA3 of the RH and LH, and the DG. Significant differences between the control and experimental groups were assessed with a Student’s t -test

    Article Snippet: In every microplate, a primary antibody for Cx 32 (anti-mouse polyclonal antibody; Zymed, CA, USA), Cx 36 (anti-rabbit polyclonal antibody; Santa Cruz Biotechnology, CA, USA) and Cx 43 (anti-rabbit polyclonal antibody; Santa Cruz Biotechnology, CA, USA), diluted 1:500 with PBS (0.12 M, pH 7.3), was incubated overnight at 4 °C.

    Techniques: Immunofluorescence

    RyR2 and COX1 colocalize with Cx43 in HL-1 cells. Immunostaining for Cx43 (Sc9059) and RyR2 (middle panel) and COX1 (lower panel), in HL-1 cells. Positive control was performed by colocalization analysis with F-actin, stained with Phalloidin (upper pannel, Cx43 staining with 610062). Nuclei were stained with DAPI. Scale bars, 20 μm. Arrows indicate colocalization spots. Colocalization pixel map is depicted on the right side.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart *

    doi: 10.1074/mcp.M115.052894

    Figure Lengend Snippet: RyR2 and COX1 colocalize with Cx43 in HL-1 cells. Immunostaining for Cx43 (Sc9059) and RyR2 (middle panel) and COX1 (lower panel), in HL-1 cells. Positive control was performed by colocalization analysis with F-actin, stained with Phalloidin (upper pannel, Cx43 staining with 610062). Nuclei were stained with DAPI. Scale bars, 20 μm. Arrows indicate colocalization spots. Colocalization pixel map is depicted on the right side.

    Article Snippet: Incubation with primary antibodies was performed using antibodies against Cx43 (rabbit polyclonal (sc-9059, Santa Cruz Biotechnology, Dallas, TX) or mouse monoclonal (610062, BD Transduction Laboratories, San Jose, CA), as applicable), β-actin (A5441, Sigma-Aldrich, St. Louis, MO), Clathrin Heavy chain (610590, BD Transduction Laboratories), Cytochrome c oxidase subunit 1 (ab14705, Abcam), Mitofusin 1 (sc-50330, Santa Cruz Biotechnology) and Ryanodine receptor 2 (C3–33, Sigma-Aldrich).

    Techniques: Immunostaining, Positive Control, Staining

    RyR2, Mfn1 and COX1 colocalize with Cx43 in the rat heart. Hearts from 10-week-old Wistar rats were maintained using a Langendorff apparatus for 10 min, followed by 20 min of perfusion (CT). Cryosections of control or ischemic hearts were immunostained using antibodies against ( A ) Cx43 (Sc9059) and RyR2 (upper panel), Cx43 (610062) and Mfn1 (middle panel), and Cx43 (Sc9059) and COX1 (lower panel). B , Cx43 (Sc9059) and Clathrin heavy chain (upper panel) or β-actin (lower panel). Nuclei were stained with DAPI. Scale bars, 25 μm. Arrows indicate colocalization spots. Colocalization pixel map is depicted on the right side.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart *

    doi: 10.1074/mcp.M115.052894

    Figure Lengend Snippet: RyR2, Mfn1 and COX1 colocalize with Cx43 in the rat heart. Hearts from 10-week-old Wistar rats were maintained using a Langendorff apparatus for 10 min, followed by 20 min of perfusion (CT). Cryosections of control or ischemic hearts were immunostained using antibodies against ( A ) Cx43 (Sc9059) and RyR2 (upper panel), Cx43 (610062) and Mfn1 (middle panel), and Cx43 (Sc9059) and COX1 (lower panel). B , Cx43 (Sc9059) and Clathrin heavy chain (upper panel) or β-actin (lower panel). Nuclei were stained with DAPI. Scale bars, 25 μm. Arrows indicate colocalization spots. Colocalization pixel map is depicted on the right side.

    Article Snippet: Incubation with primary antibodies was performed using antibodies against Cx43 (rabbit polyclonal (sc-9059, Santa Cruz Biotechnology, Dallas, TX) or mouse monoclonal (610062, BD Transduction Laboratories, San Jose, CA), as applicable), β-actin (A5441, Sigma-Aldrich, St. Louis, MO), Clathrin Heavy chain (610590, BD Transduction Laboratories), Cytochrome c oxidase subunit 1 (ab14705, Abcam), Mitofusin 1 (sc-50330, Santa Cruz Biotechnology) and Ryanodine receptor 2 (C3–33, Sigma-Aldrich).

    Techniques: Staining

    AP-SWATH approach for the study of the dynamic interactome of Cx43 in heart. A , Immunoblotting detection of the immunopurified Cx43 (Cx43 IP versus control IP). B , SWATH quantification of Cx43 IP in each condition. Data are presented as boxplots of the normalized values to internal standard (IS). Student t test was applied. ****ρ

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart *

    doi: 10.1074/mcp.M115.052894

    Figure Lengend Snippet: AP-SWATH approach for the study of the dynamic interactome of Cx43 in heart. A , Immunoblotting detection of the immunopurified Cx43 (Cx43 IP versus control IP). B , SWATH quantification of Cx43 IP in each condition. Data are presented as boxplots of the normalized values to internal standard (IS). Student t test was applied. ****ρ

    Article Snippet: Incubation with primary antibodies was performed using antibodies against Cx43 (rabbit polyclonal (sc-9059, Santa Cruz Biotechnology, Dallas, TX) or mouse monoclonal (610062, BD Transduction Laboratories, San Jose, CA), as applicable), β-actin (A5441, Sigma-Aldrich, St. Louis, MO), Clathrin Heavy chain (610590, BD Transduction Laboratories), Cytochrome c oxidase subunit 1 (ab14705, Abcam), Mitofusin 1 (sc-50330, Santa Cruz Biotechnology) and Ryanodine receptor 2 (C3–33, Sigma-Aldrich).

    Techniques:

    Gap junctions are composed of Cx26 and Cx43. (A) Cx32 is expressed neither in BxC nor in Bx2.0. Cells were fixed and processed for indirect immunofluorescence using a specific polyclonal anti-Cx32 antibody as described in Materials and Methods. Where

    Journal:

    Article Title: Restoration of Functional Gap Junctions through Internal Ribosome Entry Site-Dependent Synthesis of Endogenous Connexins in Density-Inhibited Cancer Cells

    doi: 10.1128/MCB.25.10.4034-4045.2005

    Figure Lengend Snippet: Gap junctions are composed of Cx26 and Cx43. (A) Cx32 is expressed neither in BxC nor in Bx2.0. Cells were fixed and processed for indirect immunofluorescence using a specific polyclonal anti-Cx32 antibody as described in Materials and Methods. Where

    Article Snippet: The anti-Cx43 rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology.

    Techniques: Immunofluorescence

    MiR-23a regulated the expression of Cx43 and induced gap junction remodeling. (A) Complementarity between miR-23a seed sequence (5'end 7 nucleotides) and the 3'UTR of rat's GJA1 mRNA predicted by a computational and bioinformatics-based approach using the TargetScan database. The miRNA-masking antisense (ODN-23a) was designed to be fully complementary to the miR-23a targeting sequence on 3'UTR of GJA1. Watson-Crick complementarity is connected by “|”. (B) Luciferase reporter assay for determining interactions between miR-23a and its binding sites at the 3'UTR of GJA1 or mutated 3'UTR of GJA1 in HEK293 cells. Cells were transfected with negative control (NC), miR-23a or miR-23a+AMO-23a. (C) The representative electron micrographs of NRVCs. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. Down: magnified images of gap junctions from dashed boxes of above images. (D) The miR-23a expression level in cultured primary neonatal rat ventricular cells (NRVCs) after transfection. (E) The size of gap junction per intercalated discs. (F) Effects of miR-23a on endogenous Cx43 protein level in NRVCs were determined by western blot analysis. Cells were transfected with NC, miR-23a, AMO-23a or miR-23a+AMO-23a. (n=3 batches of cells for each group). * P

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1

    doi: 10.7150/ijbs.10930

    Figure Lengend Snippet: MiR-23a regulated the expression of Cx43 and induced gap junction remodeling. (A) Complementarity between miR-23a seed sequence (5'end 7 nucleotides) and the 3'UTR of rat's GJA1 mRNA predicted by a computational and bioinformatics-based approach using the TargetScan database. The miRNA-masking antisense (ODN-23a) was designed to be fully complementary to the miR-23a targeting sequence on 3'UTR of GJA1. Watson-Crick complementarity is connected by “|”. (B) Luciferase reporter assay for determining interactions between miR-23a and its binding sites at the 3'UTR of GJA1 or mutated 3'UTR of GJA1 in HEK293 cells. Cells were transfected with negative control (NC), miR-23a or miR-23a+AMO-23a. (C) The representative electron micrographs of NRVCs. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. Down: magnified images of gap junctions from dashed boxes of above images. (D) The miR-23a expression level in cultured primary neonatal rat ventricular cells (NRVCs) after transfection. (E) The size of gap junction per intercalated discs. (F) Effects of miR-23a on endogenous Cx43 protein level in NRVCs were determined by western blot analysis. Cells were transfected with NC, miR-23a, AMO-23a or miR-23a+AMO-23a. (n=3 batches of cells for each group). * P

    Article Snippet: Specimens were incubated with primary antibodie of Cx43 (Santa Cruz, sc13558, lot# k2408, 1: 100) diluted with PBS for 3h at 37°C.

    Techniques: Expressing, Sequencing, Luciferase, Reporter Assay, Binding Assay, Transfection, Negative Control, Cell Culture, Western Blot

    E 2 delivery improved gap junction remodeling as well as Cx43 downregulation via regulating miR-23a. (A) Average serum estrogen level of experimental animals. (B-C) Representative electrocardiograms and quantitative analysis of PR intervals and QRS durations. (D) The representative electron micrographs of myocardium from 6M, 18M and 18M+E 2 rats. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. (E) The size of gap junction per intercalated discs. (F) The Cx43 protein level of experimental rat hearts. (G) The mRNA level of Cx43 in experimental rats. (H) The miR-23a level in left ventricular tissue of experimental rats. n=6 * P

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1

    doi: 10.7150/ijbs.10930

    Figure Lengend Snippet: E 2 delivery improved gap junction remodeling as well as Cx43 downregulation via regulating miR-23a. (A) Average serum estrogen level of experimental animals. (B-C) Representative electrocardiograms and quantitative analysis of PR intervals and QRS durations. (D) The representative electron micrographs of myocardium from 6M, 18M and 18M+E 2 rats. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. (E) The size of gap junction per intercalated discs. (F) The Cx43 protein level of experimental rat hearts. (G) The mRNA level of Cx43 in experimental rats. (H) The miR-23a level in left ventricular tissue of experimental rats. n=6 * P

    Article Snippet: Specimens were incubated with primary antibodie of Cx43 (Santa Cruz, sc13558, lot# k2408, 1: 100) diluted with PBS for 3h at 37°C.

    Techniques:

    E 2 prevented Cx43 repression and gap junction defects induced by miR-23a overexpression in NRVCs. (A) qRT-PCR assay revealed that E 2 treatment inhibited the expression of miR-23a in NRVCs. (B) E 2 treatment prevented the reduction of Cx43 protein level induced by miR-23a in NRVCs. (C) The size of gap junction per intercalated discs. (D) The representative electron micrographs of NRVCs. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. The gap junctions are pointed by arrows. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. n=3. * P

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1

    doi: 10.7150/ijbs.10930

    Figure Lengend Snippet: E 2 prevented Cx43 repression and gap junction defects induced by miR-23a overexpression in NRVCs. (A) qRT-PCR assay revealed that E 2 treatment inhibited the expression of miR-23a in NRVCs. (B) E 2 treatment prevented the reduction of Cx43 protein level induced by miR-23a in NRVCs. (C) The size of gap junction per intercalated discs. (D) The representative electron micrographs of NRVCs. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. The gap junctions are pointed by arrows. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. n=3. * P

    Article Snippet: Specimens were incubated with primary antibodie of Cx43 (Santa Cruz, sc13558, lot# k2408, 1: 100) diluted with PBS for 3h at 37°C.

    Techniques: Over Expression, Quantitative RT-PCR, Expressing

    Gap junction remodeling and Cx43 reduction in post-menopausal rats. (A) The representative electron micrographs of myocardium from both 6M and 18M rats. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. (B) The size of gap junction per intercalated discs. (C) Cx43 protein level in myocardium of 6M and 18M rats. (n=6). (D-E) The representative confocal images and quantitative analysis of Cx43 immunofluorescence activity of 6M and 18M rats. The values in column are normalized to 6M. n=3, * P

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1

    doi: 10.7150/ijbs.10930

    Figure Lengend Snippet: Gap junction remodeling and Cx43 reduction in post-menopausal rats. (A) The representative electron micrographs of myocardium from both 6M and 18M rats. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. (B) The size of gap junction per intercalated discs. (C) Cx43 protein level in myocardium of 6M and 18M rats. (n=6). (D-E) The representative confocal images and quantitative analysis of Cx43 immunofluorescence activity of 6M and 18M rats. The values in column are normalized to 6M. n=3, * P

    Article Snippet: Specimens were incubated with primary antibodie of Cx43 (Santa Cruz, sc13558, lot# k2408, 1: 100) diluted with PBS for 3h at 37°C.

    Techniques: Immunofluorescence, Activity Assay

    E 2 deficiency caused gap junction remodeling and Cx43 reduction in OVX rats. (A) The representative electron micrographs of myocardium from Sham, OVX and OVX+E 2 rats. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Down: Magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. (B) The size of gap junction per intercalated discs. (C) Cx43 protein level in myocardium of Sham, OVX and OVX+E 2 rats. (n=6). (D, E) The representative confocal images and quantitative analysis of Cx43 immunofluorescence activity of Sham, OVX and OVX+E 2 rats. (F) The mRNA level of Cx43 in experimental rats' hearts. The values in column are normalized to Sham (n=3). * P

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1

    doi: 10.7150/ijbs.10930

    Figure Lengend Snippet: E 2 deficiency caused gap junction remodeling and Cx43 reduction in OVX rats. (A) The representative electron micrographs of myocardium from Sham, OVX and OVX+E 2 rats. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Down: Magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. (B) The size of gap junction per intercalated discs. (C) Cx43 protein level in myocardium of Sham, OVX and OVX+E 2 rats. (n=6). (D, E) The representative confocal images and quantitative analysis of Cx43 immunofluorescence activity of Sham, OVX and OVX+E 2 rats. (F) The mRNA level of Cx43 in experimental rats' hearts. The values in column are normalized to Sham (n=3). * P

    Article Snippet: Specimens were incubated with primary antibodie of Cx43 (Santa Cruz, sc13558, lot# k2408, 1: 100) diluted with PBS for 3h at 37°C.

    Techniques: Immunofluorescence, Activity Assay

    MiR-23a overexpression induced Cx43 reduction and gap junction defects can be prevented by ODN. ODN was designed to be fully complementary to the miR-23a targeting sequence on 3'UTR of GJA1 . (A) The level of miR-23a in NRVCs after transfection of miR-23a and ODN-23a. (B) De-repression of Cx43 protein level by ODN-23a from miR-23a in NRVCs was determined by western blot analysis. (C) The size of gap junction per intercalated discs. (D) The representative electron micrographs of NRVCs. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. n=3. * P

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-23a Participates in Estrogen Deficiency Induced Gap Junction Remodeling of Rats by Targeting GJA1

    doi: 10.7150/ijbs.10930

    Figure Lengend Snippet: MiR-23a overexpression induced Cx43 reduction and gap junction defects can be prevented by ODN. ODN was designed to be fully complementary to the miR-23a targeting sequence on 3'UTR of GJA1 . (A) The level of miR-23a in NRVCs after transfection of miR-23a and ODN-23a. (B) De-repression of Cx43 protein level by ODN-23a from miR-23a in NRVCs was determined by western blot analysis. (C) The size of gap junction per intercalated discs. (D) The representative electron micrographs of NRVCs. Up: Full image of intercalated disk including fasciae adherentes junctions, desmosome and gap junctions. Gap junctions were pointed by arrows in dashed boxes. Down: magnified images of gap junctions from dashed boxes of above images. The gap junctions are pointed by arrows. n=3. * P

    Article Snippet: Specimens were incubated with primary antibodie of Cx43 (Santa Cruz, sc13558, lot# k2408, 1: 100) diluted with PBS for 3h at 37°C.

    Techniques: Over Expression, Sequencing, Transfection, Western Blot