confocal laser scanning fluorescence microscopy  (Carl Zeiss)

 
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    Software Module LM EM Correlative Microscopy Materials
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    Software Module LM EM Correlative Microscopy Materials Module for image recording navigation and correlation on light digital and electron microscopes Software module for ZEN core and Smartzoom 5 software Semi automatic calibration of ZEISS Correlative Microscopy specimen holder Manual calibration of custom holders and specimens with any type of fiducials Definition of regions and points of interest ROI POI ZEN core only Easy recovery of previously marked sample ROIs and POIs ZEN core only Alignment and overlay of light digital and electron microscope images and creation of new overlay images ZEN core only Automated alignment of images using algorithms for key point detection
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    Carl Zeiss confocal laser scanning fluorescence microscopy
    Subcellular localization of GFP-tagged OsIPT proteins in Arabidopsis observed by <t>confocal</t> <t>laser-scanning</t> <t>microscopy.</t> The translational fusion genes OsIPT1-GFP (A), OsIPT2-GFP (B), OsIPT3-GFP (C), GFP-OsIPT1 (D), GFP-OsIPT2 (E) and GFP-OsIPT3 (F) were transiently expressed in root epidermal cells by particle bombardment. The fusion genes pGGPS6-DsRed2 (G), a control for mitochondrial localization, and OsIPT7-GFP (H) were co-introduced into a leaf mesophyll cell. (I) Merged image of (G) and (H). The fusion genes OsIPT4-GFP (J), OsIPT5-GFP (K) and OsIPT8-GFP (L) were introduced into leaf mesophyll cells and superimposed on Chl autofluorescence (red). The fusion gene AtFSD3-DsRed2 (M, P and S), a control for nucleoid localization, was co-introduced into root epidermal cells with either OsIPT4-GFP (N), OsIPT5-GFP (Q) or OsIPT8-GFP (T). (O, R and U) Merged images of (M) and (N), (P) and (Q), and (S) and (T), respectively. Scale bars, 20 µm (A–F) and 10 µm (G–U).
    Software Module LM EM Correlative Microscopy Materials Module for image recording navigation and correlation on light digital and electron microscopes Software module for ZEN core and Smartzoom 5 software Semi automatic calibration of ZEISS Correlative Microscopy specimen holder Manual calibration of custom holders and specimens with any type of fiducials Definition of regions and points of interest ROI POI ZEN core only Easy recovery of previously marked sample ROIs and POIs ZEN core only Alignment and overlay of light digital and electron microscope images and creation of new overlay images ZEN core only Automated alignment of images using algorithms for key point detection
    https://www.bioz.com/result/confocal laser scanning fluorescence microscopy/product/Carl Zeiss
    Average 97 stars, based on 1 article reviews
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    Images

    1) Product Images from "Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal"

    Article Title: Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal

    Journal: Plant and Cell Physiology

    doi: 10.1093/pcp/pct127

    Subcellular localization of GFP-tagged OsIPT proteins in Arabidopsis observed by confocal laser-scanning microscopy. The translational fusion genes OsIPT1-GFP (A), OsIPT2-GFP (B), OsIPT3-GFP (C), GFP-OsIPT1 (D), GFP-OsIPT2 (E) and GFP-OsIPT3 (F) were transiently expressed in root epidermal cells by particle bombardment. The fusion genes pGGPS6-DsRed2 (G), a control for mitochondrial localization, and OsIPT7-GFP (H) were co-introduced into a leaf mesophyll cell. (I) Merged image of (G) and (H). The fusion genes OsIPT4-GFP (J), OsIPT5-GFP (K) and OsIPT8-GFP (L) were introduced into leaf mesophyll cells and superimposed on Chl autofluorescence (red). The fusion gene AtFSD3-DsRed2 (M, P and S), a control for nucleoid localization, was co-introduced into root epidermal cells with either OsIPT4-GFP (N), OsIPT5-GFP (Q) or OsIPT8-GFP (T). (O, R and U) Merged images of (M) and (N), (P) and (Q), and (S) and (T), respectively. Scale bars, 20 µm (A–F) and 10 µm (G–U).
    Figure Legend Snippet: Subcellular localization of GFP-tagged OsIPT proteins in Arabidopsis observed by confocal laser-scanning microscopy. The translational fusion genes OsIPT1-GFP (A), OsIPT2-GFP (B), OsIPT3-GFP (C), GFP-OsIPT1 (D), GFP-OsIPT2 (E) and GFP-OsIPT3 (F) were transiently expressed in root epidermal cells by particle bombardment. The fusion genes pGGPS6-DsRed2 (G), a control for mitochondrial localization, and OsIPT7-GFP (H) were co-introduced into a leaf mesophyll cell. (I) Merged image of (G) and (H). The fusion genes OsIPT4-GFP (J), OsIPT5-GFP (K) and OsIPT8-GFP (L) were introduced into leaf mesophyll cells and superimposed on Chl autofluorescence (red). The fusion gene AtFSD3-DsRed2 (M, P and S), a control for nucleoid localization, was co-introduced into root epidermal cells with either OsIPT4-GFP (N), OsIPT5-GFP (Q) or OsIPT8-GFP (T). (O, R and U) Merged images of (M) and (N), (P) and (Q), and (S) and (T), respectively. Scale bars, 20 µm (A–F) and 10 µm (G–U).

    Techniques Used: Confocal Laser Scanning Microscopy

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    Article Snippet: Fermentas Protein Molecular Weight Marker (Pierce™ Unstained Protein MW Marker, Thermo Fisher Scientific, Waltham, MA, USA) containing seven proteins within 14.4–116 kDa range was used in order to determine the molecular weight range of the proteins by the Image Analyzer System (Chemi Doc MP Imaging System-BioRad, Hercules, CA, USA). .. Scanning Electron Microscopy (SEM) The morphologies of the electrospun fibers were examined using a scanning electron microscope(SEM) (EVA MA 10, Zeiss, San Diego, CA, USA) at an accelerating voltage of 10 kV. ..

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    Article Snippet: .. The scanning electron microscopy analyses were performed employing a field emission scanning electron microscope (FESEM; model GeminiSEM 500, ZEISS, Oberkochen, Germany). .. Two different electron detection modes were used for FESEM imaging: (i) a secondary electron In-Lens detector, located inside the electron column, which works with low-energy secondary electrons and provides images with high resolution, and (ii) a energy selective backscattered (EsB) in-lens detector, independent of the secondary in-lens detector, which provides a pure backscattered signal with no secondary electron contamination and very low acceleration potential, providing a higher Z-contrast image than any other backscattered detector.

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    Article Snippet: .. Electron Microscopy Scanning electron microscopy (SEM) of adult parasites was performed as described for Fasciola hepatica using a DSM-950 Zeiss equipment adjusted to 25 kV. .. Transmission Electron Microscopy (TEM) of cysticerci and adult parasites was performed using samples embedded in Lowicryl and in Spurr resins , where thin sections (40–80 nm) were obtained in a microtome (Leica), mounted on 300 mesh formvar covered nickel grids and examined in a JEOL (JEM-1200 EXII) at 60–70 kv.

    Article Title: Fluoride Anion Recognition by a Multifunctional Urea Derivative: An Experimental and Theoretical Study
    Article Snippet: .. Electron Microscopy Electron microscopy images were obtained with a Merlin field emission scanning electron microscope (FESEM, resolution = 0.8 mm resolution, Carl Zeiss, Jena, Germany) equipped with a digital camera and operating at 10 kV (accelerating voltage) and 10 mA (emission current). .. Prior to imaging, a 5 nm sized Pt film was sputtered (40 mA, 30 s) on the sample placed on carbon tape.

    Microscopy:

    Article Title: Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein
    Article Snippet: Fermentas Protein Molecular Weight Marker (Pierce™ Unstained Protein MW Marker, Thermo Fisher Scientific, Waltham, MA, USA) containing seven proteins within 14.4–116 kDa range was used in order to determine the molecular weight range of the proteins by the Image Analyzer System (Chemi Doc MP Imaging System-BioRad, Hercules, CA, USA). .. Scanning Electron Microscopy (SEM) The morphologies of the electrospun fibers were examined using a scanning electron microscope(SEM) (EVA MA 10, Zeiss, San Diego, CA, USA) at an accelerating voltage of 10 kV. ..

    Article Title: One-step Synthesized Silver Nanoparticles Using Isoimperatorin: Evaluation of Photocatalytic, and Electrochemical Activities
    Article Snippet: Raman spectroscopy was performed by NICOLET-910, USA. .. The morphological analysis of NPs was evaluated by TEM microscopy (ZEISS – EM10C, Germany) and HRTEM microscopy (FEI – TEC9G20, USA) at an accelerating voltage of 100 kV and 200 kV, respectively. ..

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    Article Title: Fukuyoa paulensis gen. et sp. nov., a New Genus for the Globular Species of the Dinoflagellate Gambierdiscus (Dinophyceae)
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    Article Snippet: .. Electron Microscopy Electron microscopy images were obtained with a Merlin field emission scanning electron microscope (FESEM, resolution = 0.8 mm resolution, Carl Zeiss, Jena, Germany) equipped with a digital camera and operating at 10 kV (accelerating voltage) and 10 mA (emission current). .. Prior to imaging, a 5 nm sized Pt film was sputtered (40 mA, 30 s) on the sample placed on carbon tape.

    Transmission Electron Microscopy:

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    Light Microscopy:

    Article Title: Fukuyoa paulensis gen. et sp. nov., a New Genus for the Globular Species of the Dinoflagellate Gambierdiscus (Dinophyceae)
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    Carl Zeiss lsm 510 meta confocal laser scanning microscope
    iPLA 2 localizes to mitochondria . A , colocalization of iPLA 2 -GFP with mitochondria. pEGFP-iPLA 2 -transfected INS-1 cells were incubated for 15 min in Mito Tracker Red CMXRos, fixed with 3.8% paraformaldehyde, stained with 4′,6-diamidino-2-phenylindole, and analyzed on a Zeiss <t>LSM</t> 510 <t>META</t> confocal laser scanning microscope. Images of red (Mito Tracker) and green (iPLA 2 -GFP) fluorescence were collected by confocal microscopy. Colocalization of iPLA 2 -GFP with mitochondria appears as yellow to orange spots, depending on the ratio of the merged red and green fluorescence. B , resistance of cells expressing GFP-iPLA 2 to STS-induced apoptosis. pEGFP-iPLA 2 -transfected INS-1 cells were treated with STS and incubated with Mito Tracker Red CMXrox as in A . The cells with green and yellow to orange dots are iPLA 2 -GFP-transfected INS-1 cells. Those with red color only are non-transfected cells. C , localization of iPLA 2 -GFP in mitochondria during STS-induced apoptosis in individual cells. Merged images of red (mitochondria) and green (iPLA 2 -GFP) fluorescence were collected by confocal microscopy. The images represent at least four independent experiments with similar results.
    Lsm 510 Meta Confocal Laser Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Carl Zeiss 633 helium neon laser
    Subcellular localization of α-SYN and CYPA, Pin1, FKBP12, FKBP38, or FKBP65. Confocal microscopy of subcellular localization of α-SYN and CYPA ( A ), Pin1 ( B ), FKBP12 ( C ), FKBP38 ( D ), or FKBP65 ( E ) in cell lines that stably overexpress the respective PPIases is shown. Co-localization of FKBP38 with Mitotracker Deep Red <t>633</t> in FKBP38 overexpression cell line is shown in F . Co-localization of FKBP65 with the ER marker calnexin in FKBP65 overexpression cell line is shown in G . DAPI staining ( 1 ) is shown in blue . Immunocytochemical staining for CYPA, Pin1, FKBP12, FKBP38, or FKBP65 is shown in red ( 2 ). Immunocytochemical staining for α-SYN and calnexin or Mitotracker Deep Red 633 staining is shown in green (3). Overlap image ( 4 ). The bars represent 10 μm.
    633 Helium Neon Laser, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    iPLA 2 localizes to mitochondria . A , colocalization of iPLA 2 -GFP with mitochondria. pEGFP-iPLA 2 -transfected INS-1 cells were incubated for 15 min in Mito Tracker Red CMXRos, fixed with 3.8% paraformaldehyde, stained with 4′,6-diamidino-2-phenylindole, and analyzed on a Zeiss LSM 510 META confocal laser scanning microscope. Images of red (Mito Tracker) and green (iPLA 2 -GFP) fluorescence were collected by confocal microscopy. Colocalization of iPLA 2 -GFP with mitochondria appears as yellow to orange spots, depending on the ratio of the merged red and green fluorescence. B , resistance of cells expressing GFP-iPLA 2 to STS-induced apoptosis. pEGFP-iPLA 2 -transfected INS-1 cells were treated with STS and incubated with Mito Tracker Red CMXrox as in A . The cells with green and yellow to orange dots are iPLA 2 -GFP-transfected INS-1 cells. Those with red color only are non-transfected cells. C , localization of iPLA 2 -GFP in mitochondria during STS-induced apoptosis in individual cells. Merged images of red (mitochondria) and green (iPLA 2 -GFP) fluorescence were collected by confocal microscopy. The images represent at least four independent experiments with similar results.

    Journal: The Journal of biological chemistry

    Article Title: Calcium-independent Phospholipase A2 Localizes in and Protects Mitochondria during Apoptotic Induction by Staurosporine *

    doi: 10.1074/jbc.M604330200

    Figure Lengend Snippet: iPLA 2 localizes to mitochondria . A , colocalization of iPLA 2 -GFP with mitochondria. pEGFP-iPLA 2 -transfected INS-1 cells were incubated for 15 min in Mito Tracker Red CMXRos, fixed with 3.8% paraformaldehyde, stained with 4′,6-diamidino-2-phenylindole, and analyzed on a Zeiss LSM 510 META confocal laser scanning microscope. Images of red (Mito Tracker) and green (iPLA 2 -GFP) fluorescence were collected by confocal microscopy. Colocalization of iPLA 2 -GFP with mitochondria appears as yellow to orange spots, depending on the ratio of the merged red and green fluorescence. B , resistance of cells expressing GFP-iPLA 2 to STS-induced apoptosis. pEGFP-iPLA 2 -transfected INS-1 cells were treated with STS and incubated with Mito Tracker Red CMXrox as in A . The cells with green and yellow to orange dots are iPLA 2 -GFP-transfected INS-1 cells. Those with red color only are non-transfected cells. C , localization of iPLA 2 -GFP in mitochondria during STS-induced apoptosis in individual cells. Merged images of red (mitochondria) and green (iPLA 2 -GFP) fluorescence were collected by confocal microscopy. The images represent at least four independent experiments with similar results.

    Article Snippet: Confocal fluorescence microscopy was performed using a Zeiss LSM 510 META confocal laser scanning microscope (Carl Zeiss MicroImaging, Inc. Thornwood, NY).

    Techniques: Transfection, Incubation, Staining, Laser-Scanning Microscopy, Fluorescence, Confocal Microscopy, Expressing

    CNGB3 F525N mutation impairs cell viability with CPT-cGMP treatment. Transfected 661W cells were treated with or without 0.1 μM CPT-cGMP for 24 h. After treatment, cells were labeled according to the LIVE/DEAD assay protocol: vital cells were stained by calcein AM and show green fluorescence ( A , C , E , and G ); damaged cells were penetrated by ethidium homodimer and show red fluorescent nuclei ( B , D , F and H ). Fluorescent images were obtained using a Zeiss LSM 510 confocal laser-scanning microscope as described in the Methods section.

    Journal: Molecular Vision

    Article Title: Disease-associated mutations in CNGB3 promote cytotoxicity in photoreceptor-derived cells

    doi:

    Figure Lengend Snippet: CNGB3 F525N mutation impairs cell viability with CPT-cGMP treatment. Transfected 661W cells were treated with or without 0.1 μM CPT-cGMP for 24 h. After treatment, cells were labeled according to the LIVE/DEAD assay protocol: vital cells were stained by calcein AM and show green fluorescence ( A , C , E , and G ); damaged cells were penetrated by ethidium homodimer and show red fluorescent nuclei ( B , D , F and H ). Fluorescent images were obtained using a Zeiss LSM 510 confocal laser-scanning microscope as described in the Methods section.

    Article Snippet: Images were obtained using a 10× objective on an Axiovert 200M inverted microscope equipped with a Zeiss LSM 510 confocal laser-scanning system and a krypton-argon laser.

    Techniques: Mutagenesis, Cycling Probe Technology, Transfection, Labeling, Live Dead Assay, Staining, Fluorescence, Laser-Scanning Microscopy

    CNGB3 F525N mutation increases annexin V–positive cells compared to wild-type channels. Transfected cells were treated with 0.1 μM CPT-cGMP for 24 h. For determination of cell death, cells were stained with fluorescein-labeled annexin V, a protein with a high affinity for phosphatidylserine ( A and B ). Cells were also counterstained with DAPI to count nuclei ( C and D ), and the two images were merged to count annexin V–positive cells ( E and F ). G : Summary bar graph for annexin V staining of cells transfected with control plasmid, wild-type CNGB3 plus CNGA3, or CNGB3-F525N plus CNGA3 plasmids. Fluorescent images were obtained using a Zeiss LSM 510 confocal system as described in the Methods. Scale bar in F (applies to A - F ), 100 µm.

    Journal: Molecular Vision

    Article Title: Disease-associated mutations in CNGB3 promote cytotoxicity in photoreceptor-derived cells

    doi:

    Figure Lengend Snippet: CNGB3 F525N mutation increases annexin V–positive cells compared to wild-type channels. Transfected cells were treated with 0.1 μM CPT-cGMP for 24 h. For determination of cell death, cells were stained with fluorescein-labeled annexin V, a protein with a high affinity for phosphatidylserine ( A and B ). Cells were also counterstained with DAPI to count nuclei ( C and D ), and the two images were merged to count annexin V–positive cells ( E and F ). G : Summary bar graph for annexin V staining of cells transfected with control plasmid, wild-type CNGB3 plus CNGA3, or CNGB3-F525N plus CNGA3 plasmids. Fluorescent images were obtained using a Zeiss LSM 510 confocal system as described in the Methods. Scale bar in F (applies to A - F ), 100 µm.

    Article Snippet: Images were obtained using a 10× objective on an Axiovert 200M inverted microscope equipped with a Zeiss LSM 510 confocal laser-scanning system and a krypton-argon laser.

    Techniques: Mutagenesis, Transfection, Cycling Probe Technology, Staining, Labeling, Plasmid Preparation

    Molecular analysis of Met kinetic signature- mRNA and protein levels of selected genes in high and low Met expressing cells. (A) Total cellular RNA, was isolated from low (MCF7) and high Met (MDA231) cell cultures and mRNA expression of Met, Survivin, Pbk, Cyclin E1 and Ki67 was evaluated by quantitative real time PCR and compared mRNA levels of the housekeeping GAPDH gene. The primers used for the quantification of gene expression are listed in Table S2 . A gray box denotes MCF7 cell line samples and a black box denotes MDA231 cell line samples (B) Samples from low (MCF7) and high Met (MDA231) cells were subjected to western blot (WB) analysis, before and 15 min and 60 min after treatment with HGF/SF, using antibodies against Met and activated Met (p-Met) and (C) antibodies against ERK K-23, p-ERK E-4, E-Cadherin, Survivin and Actin C4. (D, E) Subcellular localization of survivin in fluorescence (IF) analysis of Low (MCF7) and high Met (MDA231) cells after treatment with HGF/SF at 0 min, 10 min, 30 min and 24 h. The cells were Immunostained using anti-Survivin antibody. Immunofluorescence was examined using a 510 Meta Zeiss confocal laser scanning microscope (CLSM). Survivin quantification was performed on at least five confocal images per slide. Cell outline was defined based on Nomarski images; nuclei were defined based on the DAPI staining. Average pixel intensity was calculated separately for the nucleus and cytoplasm areas. (F) IF analysis of temporal kinetics of Survivin protein expression following treatment with HGF/SF .

    Journal: PLoS ONE

    Article Title: Met Kinetic Signature Derived from the Response to HGF/SF in a Cellular Model Predicts Breast Cancer Patient Survival

    doi: 10.1371/journal.pone.0045969

    Figure Lengend Snippet: Molecular analysis of Met kinetic signature- mRNA and protein levels of selected genes in high and low Met expressing cells. (A) Total cellular RNA, was isolated from low (MCF7) and high Met (MDA231) cell cultures and mRNA expression of Met, Survivin, Pbk, Cyclin E1 and Ki67 was evaluated by quantitative real time PCR and compared mRNA levels of the housekeeping GAPDH gene. The primers used for the quantification of gene expression are listed in Table S2 . A gray box denotes MCF7 cell line samples and a black box denotes MDA231 cell line samples (B) Samples from low (MCF7) and high Met (MDA231) cells were subjected to western blot (WB) analysis, before and 15 min and 60 min after treatment with HGF/SF, using antibodies against Met and activated Met (p-Met) and (C) antibodies against ERK K-23, p-ERK E-4, E-Cadherin, Survivin and Actin C4. (D, E) Subcellular localization of survivin in fluorescence (IF) analysis of Low (MCF7) and high Met (MDA231) cells after treatment with HGF/SF at 0 min, 10 min, 30 min and 24 h. The cells were Immunostained using anti-Survivin antibody. Immunofluorescence was examined using a 510 Meta Zeiss confocal laser scanning microscope (CLSM). Survivin quantification was performed on at least five confocal images per slide. Cell outline was defined based on Nomarski images; nuclei were defined based on the DAPI staining. Average pixel intensity was calculated separately for the nucleus and cytoplasm areas. (F) IF analysis of temporal kinetics of Survivin protein expression following treatment with HGF/SF .

    Article Snippet: Slides were analyzed using a 510 Meta Zeiss confocal laser scanning microscope (CLSM).

    Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction, Western Blot, Fluorescence, Immunofluorescence, Laser-Scanning Microscopy, Confocal Laser Scanning Microscopy, Staining

    Subcellular localization of α-SYN and CYPA, Pin1, FKBP12, FKBP38, or FKBP65. Confocal microscopy of subcellular localization of α-SYN and CYPA ( A ), Pin1 ( B ), FKBP12 ( C ), FKBP38 ( D ), or FKBP65 ( E ) in cell lines that stably overexpress the respective PPIases is shown. Co-localization of FKBP38 with Mitotracker Deep Red 633 in FKBP38 overexpression cell line is shown in F . Co-localization of FKBP65 with the ER marker calnexin in FKBP65 overexpression cell line is shown in G . DAPI staining ( 1 ) is shown in blue . Immunocytochemical staining for CYPA, Pin1, FKBP12, FKBP38, or FKBP65 is shown in red ( 2 ). Immunocytochemical staining for α-SYN and calnexin or Mitotracker Deep Red 633 staining is shown in green (3). Overlap image ( 4 ). The bars represent 10 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Comparative Analysis of Different Peptidyl-Prolyl Isomerases Reveals FK506-binding Protein 12 as the Most Potent Enhancer of ?-Synuclein Aggregation *

    doi: 10.1074/jbc.M110.182303

    Figure Lengend Snippet: Subcellular localization of α-SYN and CYPA, Pin1, FKBP12, FKBP38, or FKBP65. Confocal microscopy of subcellular localization of α-SYN and CYPA ( A ), Pin1 ( B ), FKBP12 ( C ), FKBP38 ( D ), or FKBP65 ( E ) in cell lines that stably overexpress the respective PPIases is shown. Co-localization of FKBP38 with Mitotracker Deep Red 633 in FKBP38 overexpression cell line is shown in F . Co-localization of FKBP65 with the ER marker calnexin in FKBP65 overexpression cell line is shown in G . DAPI staining ( 1 ) is shown in blue . Immunocytochemical staining for CYPA, Pin1, FKBP12, FKBP38, or FKBP65 is shown in red ( 2 ). Immunocytochemical staining for α-SYN and calnexin or Mitotracker Deep Red 633 staining is shown in green (3). Overlap image ( 4 ). The bars represent 10 μm.

    Article Snippet: Fluorescence was detected with the 488 argon-ion laser, the 633 helium-neon laser and 405 diode for the DAPI staining with a laser scanning microscopy unit (LSM 710, Carl Zeiss, Jena, Germany).

    Techniques: Confocal Microscopy, Stable Transfection, Over Expression, Marker, Staining