confocal laser scanning fluorescence microscopy  (Carl Zeiss)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89

    Structured Review

    Carl Zeiss confocal laser scanning fluorescence microscopy
    Subcellular localization of GFP-tagged OsIPT proteins in Arabidopsis observed by <t>confocal</t> <t>laser-scanning</t> <t>microscopy.</t> The translational fusion genes OsIPT1-GFP (A), OsIPT2-GFP (B), OsIPT3-GFP (C), GFP-OsIPT1 (D), GFP-OsIPT2 (E) and GFP-OsIPT3 (F) were transiently expressed in root epidermal cells by particle bombardment. The fusion genes pGGPS6-DsRed2 (G), a control for mitochondrial localization, and OsIPT7-GFP (H) were co-introduced into a leaf mesophyll cell. (I) Merged image of (G) and (H). The fusion genes OsIPT4-GFP (J), OsIPT5-GFP (K) and OsIPT8-GFP (L) were introduced into leaf mesophyll cells and superimposed on Chl autofluorescence (red). The fusion gene AtFSD3-DsRed2 (M, P and S), a control for nucleoid localization, was co-introduced into root epidermal cells with either OsIPT4-GFP (N), OsIPT5-GFP (Q) or OsIPT8-GFP (T). (O, R and U) Merged images of (M) and (N), (P) and (Q), and (S) and (T), respectively. Scale bars, 20 µm (A–F) and 10 µm (G–U).
    Confocal Laser Scanning Fluorescence Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 89/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/confocal laser scanning fluorescence microscopy/product/Carl Zeiss
    Average 89 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    confocal laser scanning fluorescence microscopy - by Bioz Stars, 2020-09
    89/100 stars

    Images

    1) Product Images from "Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal"

    Article Title: Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal

    Journal: Plant and Cell Physiology

    doi: 10.1093/pcp/pct127

    Subcellular localization of GFP-tagged OsIPT proteins in Arabidopsis observed by confocal laser-scanning microscopy. The translational fusion genes OsIPT1-GFP (A), OsIPT2-GFP (B), OsIPT3-GFP (C), GFP-OsIPT1 (D), GFP-OsIPT2 (E) and GFP-OsIPT3 (F) were transiently expressed in root epidermal cells by particle bombardment. The fusion genes pGGPS6-DsRed2 (G), a control for mitochondrial localization, and OsIPT7-GFP (H) were co-introduced into a leaf mesophyll cell. (I) Merged image of (G) and (H). The fusion genes OsIPT4-GFP (J), OsIPT5-GFP (K) and OsIPT8-GFP (L) were introduced into leaf mesophyll cells and superimposed on Chl autofluorescence (red). The fusion gene AtFSD3-DsRed2 (M, P and S), a control for nucleoid localization, was co-introduced into root epidermal cells with either OsIPT4-GFP (N), OsIPT5-GFP (Q) or OsIPT8-GFP (T). (O, R and U) Merged images of (M) and (N), (P) and (Q), and (S) and (T), respectively. Scale bars, 20 µm (A–F) and 10 µm (G–U).
    Figure Legend Snippet: Subcellular localization of GFP-tagged OsIPT proteins in Arabidopsis observed by confocal laser-scanning microscopy. The translational fusion genes OsIPT1-GFP (A), OsIPT2-GFP (B), OsIPT3-GFP (C), GFP-OsIPT1 (D), GFP-OsIPT2 (E) and GFP-OsIPT3 (F) were transiently expressed in root epidermal cells by particle bombardment. The fusion genes pGGPS6-DsRed2 (G), a control for mitochondrial localization, and OsIPT7-GFP (H) were co-introduced into a leaf mesophyll cell. (I) Merged image of (G) and (H). The fusion genes OsIPT4-GFP (J), OsIPT5-GFP (K) and OsIPT8-GFP (L) were introduced into leaf mesophyll cells and superimposed on Chl autofluorescence (red). The fusion gene AtFSD3-DsRed2 (M, P and S), a control for nucleoid localization, was co-introduced into root epidermal cells with either OsIPT4-GFP (N), OsIPT5-GFP (Q) or OsIPT8-GFP (T). (O, R and U) Merged images of (M) and (N), (P) and (Q), and (S) and (T), respectively. Scale bars, 20 µm (A–F) and 10 µm (G–U).

    Techniques Used: Confocal Laser Scanning Microscopy

    2) Product Images from "Activation of Mammalian Unfolded Protein Response Is Compatible with the Quality Control System Operating in the Endoplasmic Reticulum"

    Article Title: Activation of Mammalian Unfolded Protein Response Is Compatible with the Quality Control System Operating in the Endoplasmic Reticulum

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E03-09-0693

    Analysis of the route and mechanism of ER stress-induced transport of ATF6. (A) CHO cells treated with 1 mM DTT for the indicated periods were fixed, double-stained with anti-ATF6α and anti-ERGIC-53 antibodies (a–i) or with anti-ATF6α and anti-GM130 antibodies (j–r), and then analyzed by fluorescence microscopy. ATF6α was visualized using FITC-conjugated secondary antibody (shown in green), whereas ERGIC-53 and GM130 were visualized using rhodamine-conjugated secondary antibody (shown in red). An outline of the nucleus is indicated by a white line in each cell. (B) Plasmid DNA encoding either the wild-type Sar1 [Sar1(WT)] or dominant negative mutant form of Sar1 [Sar1(DN)] was microinjected into nuclei of CHO cells expressing GFP-ATF6α (shown in green) together with TRITC-conjugated dextran (shown in red). Five hours later, cells were treated with 1 mM DTT for the indicated periods, and then analyzed by confocal laser scanning fluorescence microscopy. An outline of the nucleus is indicated by a white line in each cell.
    Figure Legend Snippet: Analysis of the route and mechanism of ER stress-induced transport of ATF6. (A) CHO cells treated with 1 mM DTT for the indicated periods were fixed, double-stained with anti-ATF6α and anti-ERGIC-53 antibodies (a–i) or with anti-ATF6α and anti-GM130 antibodies (j–r), and then analyzed by fluorescence microscopy. ATF6α was visualized using FITC-conjugated secondary antibody (shown in green), whereas ERGIC-53 and GM130 were visualized using rhodamine-conjugated secondary antibody (shown in red). An outline of the nucleus is indicated by a white line in each cell. (B) Plasmid DNA encoding either the wild-type Sar1 [Sar1(WT)] or dominant negative mutant form of Sar1 [Sar1(DN)] was microinjected into nuclei of CHO cells expressing GFP-ATF6α (shown in green) together with TRITC-conjugated dextran (shown in red). Five hours later, cells were treated with 1 mM DTT for the indicated periods, and then analyzed by confocal laser scanning fluorescence microscopy. An outline of the nucleus is indicated by a white line in each cell.

    Techniques Used: Staining, Fluorescence, Microscopy, Plasmid Preparation, Dominant Negative Mutation, Expressing

    3) Product Images from "Receptor-associated Protein Interacts with Amyloid-? Peptide and Promotes Its Cellular Uptake *"

    Article Title: Receptor-associated Protein Interacts with Amyloid-? Peptide and Promotes Its Cellular Uptake *

    Journal:

    doi: 10.1074/jbc.M109.015032

    Enhancement of Aβ cellular uptake by RAP in brain vascular smooth muscle cells. The effect of RAP on the cellular uptake of Aβ in HBVSMC was analyzed using confocal laser scanning microscopy. HBVSMC were treated with 500 n m FAM-labeled
    Figure Legend Snippet: Enhancement of Aβ cellular uptake by RAP in brain vascular smooth muscle cells. The effect of RAP on the cellular uptake of Aβ in HBVSMC was analyzed using confocal laser scanning microscopy. HBVSMC were treated with 500 n m FAM-labeled

    Techniques Used: Confocal Laser Scanning Microscopy, Labeling

    Related Articles

    Expressing:

    Article Title: Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal
    Article Snippet: .. After overnight incubation in the dark, transient expression was observed by confocal laser-scanning fluorescence microscopy (Zeiss LSM510 META, Carl Zeiss). .. The frozen roots were powdered in liquid nitrogen and then homogenized in 10 vols. of 10 mM HCl with 0.2 mM methionine sulfone as an internal control.

    Fluorescence:

    Article Title: Activation of Mammalian Unfolded Protein Response Is Compatible with the Quality Control System Operating in the Endoplasmic Reticulum
    Article Snippet: .. Primary antibodies were visualized by incubation for 1 h at 37°C with fluorescein isothiocyanate–conjugated goat anti-rabbit IgG antibody, rhodamine-conjugated goat anti-rabbit IgG antibody, or rhodamine-conjugated goat anti-mouse IgG antibody (ICN Pharmaceuticals, Costa Mesa, CA), followed by confocal laser scanning fluorescence microscopy performed using an LSM510 (Carl Zeiss, Thornwood, NY). .. GFP or one of its variants in CHO cells cultured in 35-mm glass-base dishes was visualized using an IX71 inverted fluorescent microscope (Olympus, Tokyo, Japan).

    Article Title: Receptor-associated Protein Interacts with Amyloid-? Peptide and Promotes Its Cellular Uptake *
    Article Snippet: .. After incubation with FAM-labeled Aβ40 (500 n m ) at 37 °C for 4 h in serum-free DMEM in the presence or absence of RAP (500 n m ), the fluorescence of Aβ40 was observed by confocal laser scanning fluorescence microscopy (Model LSM 510 inverted microscope, Carl Zeiss, Jena, Germany) at 488-nm argon excitation using a 510–535-nm bandpass barrier filter. ..

    Article Title: Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal
    Article Snippet: .. After overnight incubation in the dark, transient expression was observed by confocal laser-scanning fluorescence microscopy (Zeiss LSM510 META, Carl Zeiss). .. The frozen roots were powdered in liquid nitrogen and then homogenized in 10 vols. of 10 mM HCl with 0.2 mM methionine sulfone as an internal control.

    Article Title: PI3‐kinase/Akt pathway‐regulated membrane transportation of acid‐sensing ion channel 1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF‐induced HSC Activation, et al. PI3‐kinase/Akt pathway‐regulated membrane transportation of acid‐sensing ion channel 1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF‐induced HSC Activation
    Article Snippet: .. The fluorescence of intracellular Fluo‐3 was quantitated by confocal laser scanning fluorescence microscopy (Zeiss) with excitation at 488 nm and emission at 525 nm. ..

    Microscopy:

    Article Title: Activation of Mammalian Unfolded Protein Response Is Compatible with the Quality Control System Operating in the Endoplasmic Reticulum
    Article Snippet: .. Primary antibodies were visualized by incubation for 1 h at 37°C with fluorescein isothiocyanate–conjugated goat anti-rabbit IgG antibody, rhodamine-conjugated goat anti-rabbit IgG antibody, or rhodamine-conjugated goat anti-mouse IgG antibody (ICN Pharmaceuticals, Costa Mesa, CA), followed by confocal laser scanning fluorescence microscopy performed using an LSM510 (Carl Zeiss, Thornwood, NY). .. GFP or one of its variants in CHO cells cultured in 35-mm glass-base dishes was visualized using an IX71 inverted fluorescent microscope (Olympus, Tokyo, Japan).

    Article Title: Receptor-associated Protein Interacts with Amyloid-? Peptide and Promotes Its Cellular Uptake *
    Article Snippet: .. After incubation with FAM-labeled Aβ40 (500 n m ) at 37 °C for 4 h in serum-free DMEM in the presence or absence of RAP (500 n m ), the fluorescence of Aβ40 was observed by confocal laser scanning fluorescence microscopy (Model LSM 510 inverted microscope, Carl Zeiss, Jena, Germany) at 488-nm argon excitation using a 510–535-nm bandpass barrier filter. ..

    Article Title: Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal
    Article Snippet: .. After overnight incubation in the dark, transient expression was observed by confocal laser-scanning fluorescence microscopy (Zeiss LSM510 META, Carl Zeiss). .. The frozen roots were powdered in liquid nitrogen and then homogenized in 10 vols. of 10 mM HCl with 0.2 mM methionine sulfone as an internal control.

    Article Title: PI3‐kinase/Akt pathway‐regulated membrane transportation of acid‐sensing ion channel 1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF‐induced HSC Activation, et al. PI3‐kinase/Akt pathway‐regulated membrane transportation of acid‐sensing ion channel 1a/Calcium ion influx/endoplasmic reticulum stress activation on PDGF‐induced HSC Activation
    Article Snippet: .. The fluorescence of intracellular Fluo‐3 was quantitated by confocal laser scanning fluorescence microscopy (Zeiss) with excitation at 488 nm and emission at 525 nm. ..

    Article Title: Symplekin, a Constitutive Protein of Karyo- and Cytoplasmic Particles Involved in mRNA Biogenesis in Xenopus laevis Oocytes
    Article Snippet: .. Confocal laser scanning immunofluorescence microscopy was done on a Zeiss LSM 410 UV instrument (Zeiss). .. For simultaneous double-label fluorescence, an argon ion laser operating at 488 nm and a helium-neon laser operating at 543 nm were used together with a band-pass filter combination of 510–525 nm and 590–610 nm for visualization of Cy-2 and Cy-3 fluorescence.

    Article Title: E-N-cadherin heterodimers define novel adherens junctions connecting endoderm-derived cells
    Article Snippet: .. For confocal laser-scanning immunofluorescence microscopy, a microscope (LSM 510 Meta; Carl Zeiss) equipped with Plan Apochromat 63×/1.40 NA oil and Plan-Neofluar 40×/1.30 NA oil objectives was used. .. AxioVision Release 4.6.3.0 and LSM Image browser 3.2.0.115 software (both obtained from Carl Zeiss) was used for image processing, and Photoshop CS3 (Adobe) was used for final figure preparation.

    Article Title: Lipid Droplets, Perilipins and Cytokeratins - Unravelled Liaisons in Epithelium-Derived Cells
    Article Snippet: .. For confocal laser-scanning immunofluorescence microscopy, LSM 700 and LSM 780 microscopes (Carl Zeiss) were used. ..

    Article Title: Molecular Characterization of a Novel, Widespread Nuclear Protein That Colocalizes with Spliceosome Components
    Article Snippet: .. Confocal laser-scanning immunofluorescence microscopy was done on a Zeiss LSM 410 UV instrument ( Zeiss ). .. For simultaneous double-label fluorescence, an argon ion laser operating at 488 nm and a helium-neon laser operating at 543 nm were used together with a band-pass filter combination of 510–525 nm and 590–610 nm for visualization of Cy-2 and Cy-3 fluorescence, respectively.

    Incubation:

    Article Title: Activation of Mammalian Unfolded Protein Response Is Compatible with the Quality Control System Operating in the Endoplasmic Reticulum
    Article Snippet: .. Primary antibodies were visualized by incubation for 1 h at 37°C with fluorescein isothiocyanate–conjugated goat anti-rabbit IgG antibody, rhodamine-conjugated goat anti-rabbit IgG antibody, or rhodamine-conjugated goat anti-mouse IgG antibody (ICN Pharmaceuticals, Costa Mesa, CA), followed by confocal laser scanning fluorescence microscopy performed using an LSM510 (Carl Zeiss, Thornwood, NY). .. GFP or one of its variants in CHO cells cultured in 35-mm glass-base dishes was visualized using an IX71 inverted fluorescent microscope (Olympus, Tokyo, Japan).

    Article Title: Receptor-associated Protein Interacts with Amyloid-? Peptide and Promotes Its Cellular Uptake *
    Article Snippet: .. After incubation with FAM-labeled Aβ40 (500 n m ) at 37 °C for 4 h in serum-free DMEM in the presence or absence of RAP (500 n m ), the fluorescence of Aβ40 was observed by confocal laser scanning fluorescence microscopy (Model LSM 510 inverted microscope, Carl Zeiss, Jena, Germany) at 488-nm argon excitation using a 510–535-nm bandpass barrier filter. ..

    Article Title: Nitrogen-Dependent Regulation of De Novo Cytokinin Biosynthesis in Rice: The Role of Glutamine Metabolism as an Additional Signal
    Article Snippet: .. After overnight incubation in the dark, transient expression was observed by confocal laser-scanning fluorescence microscopy (Zeiss LSM510 META, Carl Zeiss). .. The frozen roots were powdered in liquid nitrogen and then homogenized in 10 vols. of 10 mM HCl with 0.2 mM methionine sulfone as an internal control.

    Inverted Microscopy:

    Article Title: Receptor-associated Protein Interacts with Amyloid-? Peptide and Promotes Its Cellular Uptake *
    Article Snippet: .. After incubation with FAM-labeled Aβ40 (500 n m ) at 37 °C for 4 h in serum-free DMEM in the presence or absence of RAP (500 n m ), the fluorescence of Aβ40 was observed by confocal laser scanning fluorescence microscopy (Model LSM 510 inverted microscope, Carl Zeiss, Jena, Germany) at 488-nm argon excitation using a 510–535-nm bandpass barrier filter. ..

    Immunofluorescence:

    Article Title: Symplekin, a Constitutive Protein of Karyo- and Cytoplasmic Particles Involved in mRNA Biogenesis in Xenopus laevis Oocytes
    Article Snippet: .. Confocal laser scanning immunofluorescence microscopy was done on a Zeiss LSM 410 UV instrument (Zeiss). .. For simultaneous double-label fluorescence, an argon ion laser operating at 488 nm and a helium-neon laser operating at 543 nm were used together with a band-pass filter combination of 510–525 nm and 590–610 nm for visualization of Cy-2 and Cy-3 fluorescence.

    Article Title: E-N-cadherin heterodimers define novel adherens junctions connecting endoderm-derived cells
    Article Snippet: .. For confocal laser-scanning immunofluorescence microscopy, a microscope (LSM 510 Meta; Carl Zeiss) equipped with Plan Apochromat 63×/1.40 NA oil and Plan-Neofluar 40×/1.30 NA oil objectives was used. .. AxioVision Release 4.6.3.0 and LSM Image browser 3.2.0.115 software (both obtained from Carl Zeiss) was used for image processing, and Photoshop CS3 (Adobe) was used for final figure preparation.

    Article Title: Lipid Droplets, Perilipins and Cytokeratins - Unravelled Liaisons in Epithelium-Derived Cells
    Article Snippet: .. For confocal laser-scanning immunofluorescence microscopy, LSM 700 and LSM 780 microscopes (Carl Zeiss) were used. ..

    Article Title: Molecular Characterization of a Novel, Widespread Nuclear Protein That Colocalizes with Spliceosome Components
    Article Snippet: .. Confocal laser-scanning immunofluorescence microscopy was done on a Zeiss LSM 410 UV instrument ( Zeiss ). .. For simultaneous double-label fluorescence, an argon ion laser operating at 488 nm and a helium-neon laser operating at 543 nm were used together with a band-pass filter combination of 510–525 nm and 590–610 nm for visualization of Cy-2 and Cy-3 fluorescence, respectively.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Carl Zeiss fluorescence laser scanning confocal microscopy
    Fluorescent <t>laser</t> <t>scanning</t> <t>confocal</t> <t>microscopy</t> of human aortic adventitial fibroblasts (AoAF) encapsulated in 20 wt% RLP24-PEG hydrogel cross-linked at a 3:2 vinyl sulfone to cysteine ratio, stained using Live/Dead stains. Calcein AM (Live) stain is shown
    Fluorescence Laser Scanning Confocal Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 85/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence laser scanning confocal microscopy/product/Carl Zeiss
    Average 85 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    fluorescence laser scanning confocal microscopy - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    88
    Carl Zeiss fluorescence laser scanning confocal microscope
    Immunocytochemical localization of densin-180 at synapses in dissociated hippocampal neurons. A–C , Hippocampal neurons dissociated at E18 were grown in culture on coverslips for 14–21 d and fixed with ice-cold methanol. After coverslips were incubated for 1 hr in preblock and overnight with the indicated pairs of primary antibodies, cultures were washed three times with preblock and incubated with Cy3-conjugated goat anti-mouse and FITC-conjugated goat anti-rabbit secondary antibodies. The coverslips then were washed and mounted on slides. Procedures are described in detail under Materials and Methods. Images were taken with a Zeiss <t>laser-scanning</t> <t>fluorescence</t> <t>confocal</t> <t>microscope,</t> and images of double-labeled cells were combined and colorized with Adobe Photoshop software. Red pseudocolor represents Cy3 staining, and green represents FITC staining. Regions of overlap are yellow . A , Double-staining for synapsin I and densin-180. Cultures grown for 21 d in vitro were double-labeled with anti-synapsin I (1:1000; green ) and anti-densin-180 (M2, 1:150; red ). A combined image taken with a 63× objective is shown. The inset at left is a 3× zoom of the area included in the white box . Note the overlap in staining for densin-180 ( large arrowheads ) and synapsin I ( small arrows ). At right are the single images of densin-180 ( top ) a nd synapsin I ( bottom ). B , Double-staining for PSD-95 and densin-180. Cultures grown for 17 d in vitro were double-labeled with anti-PSD-95 (affinity-pure, 1:100; green ) and anti-densin-180 (M2, 1:150; red ). A combined image taken with a 63× objective at Zoom 1.5 is shown. The axon initial segment stained for densin-180 is indicated with an arrow . The inset at left is a 2× zoom of the area included in the white box . Note the precise colocalization of PSD-95 staining and densin-180 staining at spine-like structures along dendrites ( large arrowheads ). At right are the single images of densin-180 ( top ) and PSD-95 ( bottom ). C , Double-staining for αCaMKII and densin-180. Cultures grown for 14 d in vitro were double-labeled with anti-αCaMKII (6G9, 1:500; green ) and anti-densin-180 (CT245, 1:3000; red ). A combined image taken with a 63× objective at Zoom 2 is shown. The inset at left is a 2× zoom of the area included in the white box . Note examples of colocalization of αCaMKII staining and densin-180 staining at spine-like structures along dendrites ( large arrowheads ). At right are the single images of densin-180 ( top ) and αCaMKII ( bottom ).
    Fluorescence Laser Scanning Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence laser scanning confocal microscope/product/Carl Zeiss
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    fluorescence laser scanning confocal microscope - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Fluorescent laser scanning confocal microscopy of human aortic adventitial fibroblasts (AoAF) encapsulated in 20 wt% RLP24-PEG hydrogel cross-linked at a 3:2 vinyl sulfone to cysteine ratio, stained using Live/Dead stains. Calcein AM (Live) stain is shown

    Journal: Macromolecules

    Article Title: Resilin-Based Hybrid Hydrogels for Cardiovascular Tissue Engineering

    doi: 10.1002/macp.201200412

    Figure Lengend Snippet: Fluorescent laser scanning confocal microscopy of human aortic adventitial fibroblasts (AoAF) encapsulated in 20 wt% RLP24-PEG hydrogel cross-linked at a 3:2 vinyl sulfone to cysteine ratio, stained using Live/Dead stains. Calcein AM (Live) stain is shown

    Article Snippet: Cell viability and nuclei number were visualized using fluorescence laser scanning confocal microscopy (Zeiss LSM 510 NLO multi-photon, Carl Zeiss, Inc., Thornwood, NY).

    Techniques: Confocal Microscopy, Staining

    Confocal fluorescence microscopy to demonstrate chloroplast localization of the cNAPL polypeptide. ( a ) Schematic representation of fusion protein constructs used for cGFP-assays. ( b ) Laser scanning confocal microscopy of C.reinhardtii arg - cw15 transformants. Transformants were assayed by differential interference contrast microscopy (DIC) or by confocal fluorescence microscopy. DIC, cGFP fluorescence (green) and chlorophyll autofluorescence (red) were merged as indicated. The DIC and cGFP images are representatives of five independent experiments. Scale bar represents 5 µm. Abbreviations: ble , phleomycin resistance gene of Streptoalloteichus hindustanus ( 50 ); cgfp , synthetic GFP ( 47 ); N84, the N-terminal 84 amino acids of cNAPL; N84Δ8-39, the N-terminal 84 amino acids of cNAPL lacking 31 amino acids in the chloroplast targeting sequence; P, HSP70A / RBCS2 promoter ( 49 ); Rps18 , cytoplasmic ribosomal protein S18 of C.reinhardtii ; T, 3′-UTR of Lhcb1 or of RBCS2 gene.

    Journal: Nucleic Acids Research

    Article Title: A nucleosome assembly protein-like polypeptide binds to chloroplast group II intron RNA in Chlamydomonas reinhardtii

    doi: 10.1093/nar/gkl611

    Figure Lengend Snippet: Confocal fluorescence microscopy to demonstrate chloroplast localization of the cNAPL polypeptide. ( a ) Schematic representation of fusion protein constructs used for cGFP-assays. ( b ) Laser scanning confocal microscopy of C.reinhardtii arg - cw15 transformants. Transformants were assayed by differential interference contrast microscopy (DIC) or by confocal fluorescence microscopy. DIC, cGFP fluorescence (green) and chlorophyll autofluorescence (red) were merged as indicated. The DIC and cGFP images are representatives of five independent experiments. Scale bar represents 5 µm. Abbreviations: ble , phleomycin resistance gene of Streptoalloteichus hindustanus ( 50 ); cgfp , synthetic GFP ( 47 ); N84, the N-terminal 84 amino acids of cNAPL; N84Δ8-39, the N-terminal 84 amino acids of cNAPL lacking 31 amino acids in the chloroplast targeting sequence; P, HSP70A / RBCS2 promoter ( 49 ); Rps18 , cytoplasmic ribosomal protein S18 of C.reinhardtii ; T, 3′-UTR of Lhcb1 or of RBCS2 gene.

    Article Snippet: Laser scanning confocal fluorescence microscopy The fluorescence emissions of transformed C.reinhardtii cells were analysed by LSCFM, using a Zeiss LSM 510 META microscopy system (Carl Zeiss, Jena, Germany) based on an Axiovert inverted microscope. cGFP and plastids were excited with the 488 nm line of an argon-ion laser.

    Techniques: Fluorescence, Microscopy, Construct, Confocal Microscopy, Sequencing

    Immunocytochemical localization of densin-180 at synapses in dissociated hippocampal neurons. A–C , Hippocampal neurons dissociated at E18 were grown in culture on coverslips for 14–21 d and fixed with ice-cold methanol. After coverslips were incubated for 1 hr in preblock and overnight with the indicated pairs of primary antibodies, cultures were washed three times with preblock and incubated with Cy3-conjugated goat anti-mouse and FITC-conjugated goat anti-rabbit secondary antibodies. The coverslips then were washed and mounted on slides. Procedures are described in detail under Materials and Methods. Images were taken with a Zeiss laser-scanning fluorescence confocal microscope, and images of double-labeled cells were combined and colorized with Adobe Photoshop software. Red pseudocolor represents Cy3 staining, and green represents FITC staining. Regions of overlap are yellow . A , Double-staining for synapsin I and densin-180. Cultures grown for 21 d in vitro were double-labeled with anti-synapsin I (1:1000; green ) and anti-densin-180 (M2, 1:150; red ). A combined image taken with a 63× objective is shown. The inset at left is a 3× zoom of the area included in the white box . Note the overlap in staining for densin-180 ( large arrowheads ) and synapsin I ( small arrows ). At right are the single images of densin-180 ( top ) a nd synapsin I ( bottom ). B , Double-staining for PSD-95 and densin-180. Cultures grown for 17 d in vitro were double-labeled with anti-PSD-95 (affinity-pure, 1:100; green ) and anti-densin-180 (M2, 1:150; red ). A combined image taken with a 63× objective at Zoom 1.5 is shown. The axon initial segment stained for densin-180 is indicated with an arrow . The inset at left is a 2× zoom of the area included in the white box . Note the precise colocalization of PSD-95 staining and densin-180 staining at spine-like structures along dendrites ( large arrowheads ). At right are the single images of densin-180 ( top ) and PSD-95 ( bottom ). C , Double-staining for αCaMKII and densin-180. Cultures grown for 14 d in vitro were double-labeled with anti-αCaMKII (6G9, 1:500; green ) and anti-densin-180 (CT245, 1:3000; red ). A combined image taken with a 63× objective at Zoom 2 is shown. The inset at left is a 2× zoom of the area included in the white box . Note examples of colocalization of αCaMKII staining and densin-180 staining at spine-like structures along dendrites ( large arrowheads ). At right are the single images of densin-180 ( top ) and αCaMKII ( bottom ).

    Journal: The Journal of Neuroscience

    Article Title: Characterization of Densin-180, a New Brain-Specific Synaptic Protein of the O-Sialoglycoprotein Family

    doi: 10.1523/JNEUROSCI.16-21-06839.1996

    Figure Lengend Snippet: Immunocytochemical localization of densin-180 at synapses in dissociated hippocampal neurons. A–C , Hippocampal neurons dissociated at E18 were grown in culture on coverslips for 14–21 d and fixed with ice-cold methanol. After coverslips were incubated for 1 hr in preblock and overnight with the indicated pairs of primary antibodies, cultures were washed three times with preblock and incubated with Cy3-conjugated goat anti-mouse and FITC-conjugated goat anti-rabbit secondary antibodies. The coverslips then were washed and mounted on slides. Procedures are described in detail under Materials and Methods. Images were taken with a Zeiss laser-scanning fluorescence confocal microscope, and images of double-labeled cells were combined and colorized with Adobe Photoshop software. Red pseudocolor represents Cy3 staining, and green represents FITC staining. Regions of overlap are yellow . A , Double-staining for synapsin I and densin-180. Cultures grown for 21 d in vitro were double-labeled with anti-synapsin I (1:1000; green ) and anti-densin-180 (M2, 1:150; red ). A combined image taken with a 63× objective is shown. The inset at left is a 3× zoom of the area included in the white box . Note the overlap in staining for densin-180 ( large arrowheads ) and synapsin I ( small arrows ). At right are the single images of densin-180 ( top ) a nd synapsin I ( bottom ). B , Double-staining for PSD-95 and densin-180. Cultures grown for 17 d in vitro were double-labeled with anti-PSD-95 (affinity-pure, 1:100; green ) and anti-densin-180 (M2, 1:150; red ). A combined image taken with a 63× objective at Zoom 1.5 is shown. The axon initial segment stained for densin-180 is indicated with an arrow . The inset at left is a 2× zoom of the area included in the white box . Note the precise colocalization of PSD-95 staining and densin-180 staining at spine-like structures along dendrites ( large arrowheads ). At right are the single images of densin-180 ( top ) and PSD-95 ( bottom ). C , Double-staining for αCaMKII and densin-180. Cultures grown for 14 d in vitro were double-labeled with anti-αCaMKII (6G9, 1:500; green ) and anti-densin-180 (CT245, 1:3000; red ). A combined image taken with a 63× objective at Zoom 2 is shown. The inset at left is a 2× zoom of the area included in the white box . Note examples of colocalization of αCaMKII staining and densin-180 staining at spine-like structures along dendrites ( large arrowheads ). At right are the single images of densin-180 ( top ) and αCaMKII ( bottom ).

    Article Snippet: Cultures were viewed in a fluorescence laser-scanning confocal microscope (Zeiss LSM310, Oberkochen, Germany).

    Techniques: Incubation, Fluorescence, Microscopy, Labeling, Software, Staining, Double Staining, In Vitro