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Contextual fear <t>conditioning</t> performance. (A) Schematic diagram of the CFC paradigm showing the single-shock training session and the memory test 24 h later. (B) Learning curve across training days (D1–D3). Saline- and 25 mg/kg KA treated mice showed a progressive increase in freezing behavior, whereas 5 mg/kg KA-administered mice showed a learning delay as they displayed a similar % in freezing time as on Day 1 (* p < 0.05 vs. Sal and 25 mg/kg KA at D2; gray box indicates this time point). (C) Freezing behavior on D2. Animals from the 5 mg/kg KA group exhibited significantly lower freezing compared to the saline and 25 mg/kg groups (* p < 0.05). (D) Freezing levels during the memory test. No significant differences were observed among groups. Data are expressed as mean ± SEM. n = 16 (Sal), n = 14 (5 mg/kg KA), n = 15 (25 mg/kg KA).
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Contextual fear <t>conditioning</t> performance. (A) Schematic diagram of the CFC paradigm showing the single-shock training session and the memory test 24 h later. (B) Learning curve across training days (D1–D3). Saline- and 25 mg/kg KA treated mice showed a progressive increase in freezing behavior, whereas 5 mg/kg KA-administered mice showed a learning delay as they displayed a similar % in freezing time as on Day 1 (* p < 0.05 vs. Sal and 25 mg/kg KA at D2; gray box indicates this time point). (C) Freezing behavior on D2. Animals from the 5 mg/kg KA group exhibited significantly lower freezing compared to the saline and 25 mg/kg groups (* p < 0.05). (D) Freezing levels during the memory test. No significant differences were observed among groups. Data are expressed as mean ± SEM. n = 16 (Sal), n = 14 (5 mg/kg KA), n = 15 (25 mg/kg KA).
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Contextual fear conditioning performance. (A) Schematic diagram of the CFC paradigm showing the single-shock training session and the memory test 24 h later. (B) Learning curve across training days (D1–D3). Saline- and 25 mg/kg KA treated mice showed a progressive increase in freezing behavior, whereas 5 mg/kg KA-administered mice showed a learning delay as they displayed a similar % in freezing time as on Day 1 (* p < 0.05 vs. Sal and 25 mg/kg KA at D2; gray box indicates this time point). (C) Freezing behavior on D2. Animals from the 5 mg/kg KA group exhibited significantly lower freezing compared to the saline and 25 mg/kg groups (* p < 0.05). (D) Freezing levels during the memory test. No significant differences were observed among groups. Data are expressed as mean ± SEM. n = 16 (Sal), n = 14 (5 mg/kg KA), n = 15 (25 mg/kg KA).

Journal: Frontiers in Cellular Neuroscience

Article Title: Acute hyperexcitability differentially affects hippocampal neurogenesis features and spatial memory

doi: 10.3389/fncel.2026.1833859

Figure Lengend Snippet: Contextual fear conditioning performance. (A) Schematic diagram of the CFC paradigm showing the single-shock training session and the memory test 24 h later. (B) Learning curve across training days (D1–D3). Saline- and 25 mg/kg KA treated mice showed a progressive increase in freezing behavior, whereas 5 mg/kg KA-administered mice showed a learning delay as they displayed a similar % in freezing time as on Day 1 (* p < 0.05 vs. Sal and 25 mg/kg KA at D2; gray box indicates this time point). (C) Freezing behavior on D2. Animals from the 5 mg/kg KA group exhibited significantly lower freezing compared to the saline and 25 mg/kg groups (* p < 0.05). (D) Freezing levels during the memory test. No significant differences were observed among groups. Data are expressed as mean ± SEM. n = 16 (Sal), n = 14 (5 mg/kg KA), n = 15 (25 mg/kg KA).

Article Snippet: The test was performed in a mouse conditioning chamber (25 cm × 25 cm × 20 cm) (San Diego Instruments, San Diego, CA, USA) with transparent acrylic walls and a metal rod floor.

Techniques: Saline