concanavalin a (Thermo Fisher)

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Concanavalin A Alexa Fluor 488 Conjugate

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Catalog Number:

c11252

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Labeling Detection Products

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Structured Review

Thermo Fisher concanavalin a

Inhibitory effects on Candida biofilm. 2D confocal imaging of C. albicans CBS 562 biofilm treated with (a) vehicle (propylene glycol, 6.25% v/v); (b) standard antifungal (nystatin); (c) C. articulatus crude oil; and (d) C. sativum crude oil. The structures depicted in green (Concanavalin A, Alexa Fluor 488 Conjugate) represent the yeast cell wall and those depicted in yellow (FUN 1 Cell Stain) are nonviable cells, metabolically inactive (arrow 2). The viable cells, in turn, convert the dye FUN-1 to red fluorescent aggregates (arrow 1) (40x optical magnitude). <t>Concanavalin</t> A selectively binds to polysaccharides, including alpha-mannopyranosyl and alpha-glucopyranosyl residues, and gives a green fluorescence. FUN-1 is a fluorescent dye taken up by yeast cells; in the presence of metabolic viability it is converted from a diffuse yellow cytoplasmic stain to red [ 15 ]. It can be noted that C. sativum essential oil drastically affected the viability of C. albicans cells when compared to the vehicle and standard antifungal.

Concanavalin A Con A is one of the most widely used lectins in cell biology Our Alexa Fluor 488 conjugate of Con A exhibits the bright green fluorescence of the Alexa Fluor 488 dye absorption emission maxima 495 519 nm Alexa Fluor 488 Con A selectively binds to a mannopyranosyl and a glucopyranosyl residues
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Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99

concanavalin a - by Bioz Stars, 2021-03

95/100 stars

Images

1) Product Images from "The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis"

Article Title: The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2015/871316

Figure Legend Snippet: Inhibitory effects on Candida biofilm. 2D confocal imaging of C. albicans CBS 562 biofilm treated with (a) vehicle (propylene glycol, 6.25% v/v); (b) standard antifungal (nystatin); (c) C. articulatus crude oil; and (d) C. sativum crude oil. The structures depicted in green (Concanavalin A, Alexa Fluor 488 Conjugate) represent the yeast cell wall and those depicted in yellow (FUN 1 Cell Stain) are nonviable cells, metabolically inactive (arrow 2). The viable cells, in turn, convert the dye FUN-1 to red fluorescent aggregates (arrow 1) (40x optical magnitude). Concanavalin A selectively binds to polysaccharides, including alpha-mannopyranosyl and alpha-glucopyranosyl residues, and gives a green fluorescence. FUN-1 is a fluorescent dye taken up by yeast cells; in the presence of metabolic viability it is converted from a diffuse yellow cytoplasmic stain to red [ 15 ]. It can be noted that C. sativum essential oil drastically affected the viability of C. albicans cells when compared to the vehicle and standard antifungal.

Techniques Used: Imaging, Staining, Metabolic Labelling, Fluorescence

2) Product Images from "Regulation of Cell Surface CB2 Receptor during Human B Cell Activation and Differentiation"

Article Title: Regulation of Cell Surface CB2 Receptor during Human B Cell Activation and Differentiation

Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

doi: 10.1007/s11481-017-9744-7

Intracellular CB 2 is expressed in a diffuse but punctate pattern and demonstrates co-localization with endoplasmic reticulum compartments (a) CD20 + /CD3 − B cells purified from peripheral blood, cells prepared from the SUDHL-4 lymphoma cell line, and 293T/CB 2 -GFP cells were fixed, permeabilized, and stained with an Alexa Fluor® 647-conjugated mAb against CB 2 protein (displayed as magenta) and mounted with SlowFade® Diamond Antifade Mountant with DAPI (blue) prior to examination by confocal fluorescence microscopy. Magnification 63X; Scale Bar 25 μm;10–20 sections/cell with an SP5 blue confocal microscope. (b) 293T/CB 2 -GFP cells were grown on poly-l-lysine coated coverslips, fixed, permeabilized and stained with either Concanavalin A (ER, red), MitoTracker (mitochondria, red), or LysoTracker (lysosome, red) prior to mounting with SlowFade® Diamond Antifade Mountant with DAPI (blue). CB 2 and mitochondrial stained cells were fixed with 1% paraformaldehyde for 20 min at 4°C and later imaged. ER and lysosomal stained cells were imaged immediately without fixation. Cells were imaged in 10–20 sections with an SP5 blue confocal microscope. Magnification 63X; Scale Bar 25 μm. (c) For co-localization imaging with mitochondrial markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were first stained with MitoTracker (red) for 2 hr at 37°C, then fixed, permeabilized, and stained with anti-CB 2 mAb (green) for 30 min at 4°C. Cells were fixed, mounted and imaged in 10–20 sections with an SP5 blue confocal microscope (top) . For co-localization with ER markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were fixed, permeabilized, and stained with anti-CB 2 mAb (green) and Concanavalin A (red) for 60 min at 4°C. Cells were immediately mounted without fixation and imaged in 10–20 sections with an SP5 blue confocal microscope (bottom) . Magnification 63X; Scale bar 25 μm.

Figure Legend Snippet: Intracellular CB 2 is expressed in a diffuse but punctate pattern and demonstrates co-localization with endoplasmic reticulum compartments (a) CD20 + /CD3 − B cells purified from peripheral blood, cells prepared from the SUDHL-4 lymphoma cell line, and 293T/CB 2 -GFP cells were fixed, permeabilized, and stained with an Alexa Fluor® 647-conjugated mAb against CB 2 protein (displayed as magenta) and mounted with SlowFade® Diamond Antifade Mountant with DAPI (blue) prior to examination by confocal fluorescence microscopy. Magnification 63X; Scale Bar 25 μm;10–20 sections/cell with an SP5 blue confocal microscope. (b) 293T/CB 2 -GFP cells were grown on poly-l-lysine coated coverslips, fixed, permeabilized and stained with either Concanavalin A (ER, red), MitoTracker (mitochondria, red), or LysoTracker (lysosome, red) prior to mounting with SlowFade® Diamond Antifade Mountant with DAPI (blue). CB 2 and mitochondrial stained cells were fixed with 1% paraformaldehyde for 20 min at 4°C and later imaged. ER and lysosomal stained cells were imaged immediately without fixation. Cells were imaged in 10–20 sections with an SP5 blue confocal microscope. Magnification 63X; Scale Bar 25 μm. (c) For co-localization imaging with mitochondrial markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were first stained with MitoTracker (red) for 2 hr at 37°C, then fixed, permeabilized, and stained with anti-CB 2 mAb (green) for 30 min at 4°C. Cells were fixed, mounted and imaged in 10–20 sections with an SP5 blue confocal microscope (top) . For co-localization with ER markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were fixed, permeabilized, and stained with anti-CB 2 mAb (green) and Concanavalin A (red) for 60 min at 4°C. Cells were immediately mounted without fixation and imaged in 10–20 sections with an SP5 blue confocal microscope (bottom) . Magnification 63X; Scale bar 25 μm.

Techniques Used: Purification, Staining, Fluorescence, Microscopy, Imaging

Staining:

Article Title: Theranostic Sorafenib-Loaded Polymeric Nanocarriers Manufactured by Enhanced Gadolinium Conjugation Techniques
Article Snippet: To further analyze cellular uptake and intracellular localization, fluorescence microscopy of HepG2 cells plated on cover slides and incubated with either Cy5-labeled PLGA or PEG-PLGA NP for 4 h and 24 h was employed. .. Membrane staining was performed by using Alexa488 concanavalin A (2.5 µg/mL; Thermo Fisher Scientific, Schwerte, Germany) and nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) solution (Merck, Darmstadt, Germany). .. Finally, slides were mounted with Vectashield mounting medium (Biozol, Eching, Germany) and images were obtained using an AxioImager Z1 microscope and Axiovision 4.6 software (Carl Zeiss, Jena, Germany).

Article Title: An In Vitro Model for Oral Mixed Biofilms of Candida albicans and Streptococcus gordonii in Synthetic Saliva
Article Snippet: Confocal Scanning Laser Microscopy Biofilms were grown using 6-well plates (same as for SEM) and incubated in a 5% CO2 incubator for 24 h at 37°C. .. For confocal scanning laser microscopy (CSLM), biofilms were stained in the dark in the following order: at 37°C for 30 min with 25 μg/ml concavalin A-Alexa Fluor® 488 conjugate (Molecular Probes, Eugene, OR, USA), at room temperature for 30 min with 1X FilmTracerTM SYPRO® Ruby Biofilm Matrix Stain (Molecular Probes), and for 10 min at 37°C with 300 nM 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Molecular Probes). .. After incubation the supernatant was removed and the biofilms rinsed with 2 ml of PBS to remove non-adhered cells.

Article Title: An In Vitro Model for Candida albicans–Streptococcus gordonii Biofilms on Titanium Surfaces
Article Snippet: Samples were coated with a 60:40 gold–palladium alloy using a sputter coater and visualized using a JEOL JSM-6610 Scanning Electron Microscope (JEOL USA, Inc., Peabody, MA, USA). .. Biofilms on titanium were stained in a sequential order starting with 25 μg/mL Concanavalin A–Alexa Fluor® 488 conjugate (excitation/emission; 495/519) (Molecular Probes, Eugene, OR, USA) for 30 min at 37 °C, followed by 1 x FilmTracer™ SYPRO® Ruby Biofilm Matrix Stain (Molecular Probes) for 30 min at room temperature, and finally with 300 nM 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (excitation/emission; 358/461) (Molecular Probes, Eugene, OR, USA) for 10 min at 37 °C. ..

Incubation:

Article Title: Expression Patterns and miRNA Regulation of DNA Methyltransferases in Chicken Primordial Germ Cells
Article Snippet: The cells were incubated with 5 meC antibody (abCAM, Cambridge, UK) and diluted in blocking buffer (1∶200) at 4°C overnight. .. After primary antibody incubation, cells were washed in 1× PBS and incubated with Alexa 488 dye-conjugated secondary antibodies (Molecular Probes, Carlsbad, CA, USA) for 1 h at room temperature in the dark. .. Finally, the cells were mounted with ProLongH Gold antifade reagent with 4′-6-diamidino-2-phenylindole (DAPI, Invitrogen) and imaged with a confocal laser microscope (Carl Zeiss, Oberkochen, Germany).

Microscopy:

Whole Genome Amplification:

Article Title: An In Vitro Model for Candida albicans–Streptococcus gordonii Biofilms on Titanium Surfaces
Article Snippet: In addition, biofilm formation at different time points was monitored by fluorescence microscopy. .. Briefly, biofilms formed on titanium were stained in the dark in the following order: at 37 °C for 30 min with 25 µg/mL concavalin A–Alexa Fluor® 488 conjugate (Molecular Probes, Eugene, OR, USA) to label the fungal cell wall; at 37 °C for 20 min with 5 µg/mL wheat germ agglutinin, Texas Red™-X Conjugate (WGA, Molecular Probes) to label the bacterial cell wall (although a caveat here is that WGA can also bind to chitin in the fungal cell wall); and at 37 °C for 10 min with 300 nM 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Molecular Probes) to label nucleic acids. .. These samples were visualized using a 63× objective lens in a Leica DMR fluorescent microscope (Leica Microsystems, Buffalo Grove, IL, USA).