concanavalin a  (Thermo Fisher)


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    Name:
    Concanavalin A Alexa Fluor 488 Conjugate
    Description:
    Concanavalin A Con A is one of the most widely used lectins in cell biology Our Alexa Fluor 488 conjugate of Con A exhibits the bright green fluorescence of the Alexa Fluor 488 dye absorption emission maxima 495 519 nm Alexa Fluor 488 Con A selectively binds to a mannopyranosyl and a glucopyranosyl residues
    Catalog Number:
    c11252
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Cell Analysis|Cellular Imaging|Flow Cytometry Antibodies & Secondary Detection|General Cell Tracing|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Microbial Tracking|Flow Cytometry|Cell Tracing & Tracking
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    Structured Review

    Thermo Fisher concanavalin a
    Inhibitory effects on Candida biofilm. 2D confocal imaging of C. albicans CBS 562 biofilm treated with (a) vehicle (propylene glycol, 6.25% v/v); (b) standard antifungal (nystatin); (c) C. articulatus crude oil; and (d) C. sativum crude oil. The structures depicted in green (Concanavalin A, Alexa Fluor 488 Conjugate) represent the yeast cell wall and those depicted in yellow (FUN 1 Cell Stain) are nonviable cells, metabolically inactive (arrow 2). The viable cells, in turn, convert the dye FUN-1 to red fluorescent aggregates (arrow 1) (40x optical magnitude). <t>Concanavalin</t> A selectively binds to polysaccharides, including alpha-mannopyranosyl and alpha-glucopyranosyl residues, and gives a green fluorescence. FUN-1 is a fluorescent dye taken up by yeast cells; in the presence of metabolic viability it is converted from a diffuse yellow cytoplasmic stain to red [ 15 ]. It can be noted that C. sativum essential oil drastically affected the viability of C. albicans cells when compared to the vehicle and standard antifungal.
    Concanavalin A Con A is one of the most widely used lectins in cell biology Our Alexa Fluor 488 conjugate of Con A exhibits the bright green fluorescence of the Alexa Fluor 488 dye absorption emission maxima 495 519 nm Alexa Fluor 488 Con A selectively binds to a mannopyranosyl and a glucopyranosyl residues
    https://www.bioz.com/result/concanavalin a/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a - by Bioz Stars, 2021-03
    95/100 stars

    Images

    1) Product Images from "The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis"

    Article Title: The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2015/871316

    Inhibitory effects on Candida biofilm. 2D confocal imaging of C. albicans CBS 562 biofilm treated with (a) vehicle (propylene glycol, 6.25% v/v); (b) standard antifungal (nystatin); (c) C. articulatus crude oil; and (d) C. sativum crude oil. The structures depicted in green (Concanavalin A, Alexa Fluor 488 Conjugate) represent the yeast cell wall and those depicted in yellow (FUN 1 Cell Stain) are nonviable cells, metabolically inactive (arrow 2). The viable cells, in turn, convert the dye FUN-1 to red fluorescent aggregates (arrow 1) (40x optical magnitude). Concanavalin A selectively binds to polysaccharides, including alpha-mannopyranosyl and alpha-glucopyranosyl residues, and gives a green fluorescence. FUN-1 is a fluorescent dye taken up by yeast cells; in the presence of metabolic viability it is converted from a diffuse yellow cytoplasmic stain to red [ 15 ]. It can be noted that C. sativum essential oil drastically affected the viability of C. albicans cells when compared to the vehicle and standard antifungal.
    Figure Legend Snippet: Inhibitory effects on Candida biofilm. 2D confocal imaging of C. albicans CBS 562 biofilm treated with (a) vehicle (propylene glycol, 6.25% v/v); (b) standard antifungal (nystatin); (c) C. articulatus crude oil; and (d) C. sativum crude oil. The structures depicted in green (Concanavalin A, Alexa Fluor 488 Conjugate) represent the yeast cell wall and those depicted in yellow (FUN 1 Cell Stain) are nonviable cells, metabolically inactive (arrow 2). The viable cells, in turn, convert the dye FUN-1 to red fluorescent aggregates (arrow 1) (40x optical magnitude). Concanavalin A selectively binds to polysaccharides, including alpha-mannopyranosyl and alpha-glucopyranosyl residues, and gives a green fluorescence. FUN-1 is a fluorescent dye taken up by yeast cells; in the presence of metabolic viability it is converted from a diffuse yellow cytoplasmic stain to red [ 15 ]. It can be noted that C. sativum essential oil drastically affected the viability of C. albicans cells when compared to the vehicle and standard antifungal.

    Techniques Used: Imaging, Staining, Metabolic Labelling, Fluorescence

    2) Product Images from "Regulation of Cell Surface CB2 Receptor during Human B Cell Activation and Differentiation"

    Article Title: Regulation of Cell Surface CB2 Receptor during Human B Cell Activation and Differentiation

    Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

    doi: 10.1007/s11481-017-9744-7

    Intracellular CB 2 is expressed in a diffuse but punctate pattern and demonstrates co-localization with endoplasmic reticulum compartments (a) CD20 + /CD3 − B cells purified from peripheral blood, cells prepared from the SUDHL-4 lymphoma cell line, and 293T/CB 2 -GFP cells were fixed, permeabilized, and stained with an Alexa Fluor® 647-conjugated mAb against CB 2 protein (displayed as magenta) and mounted with SlowFade® Diamond Antifade Mountant with DAPI (blue) prior to examination by confocal fluorescence microscopy. Magnification 63X; Scale Bar 25 μm;10–20 sections/cell with an SP5 blue confocal microscope. (b) 293T/CB 2 -GFP cells were grown on poly-l-lysine coated coverslips, fixed, permeabilized and stained with either Concanavalin A (ER, red), MitoTracker (mitochondria, red), or LysoTracker (lysosome, red) prior to mounting with SlowFade® Diamond Antifade Mountant with DAPI (blue). CB 2 and mitochondrial stained cells were fixed with 1% paraformaldehyde for 20 min at 4°C and later imaged. ER and lysosomal stained cells were imaged immediately without fixation. Cells were imaged in 10–20 sections with an SP5 blue confocal microscope. Magnification 63X; Scale Bar 25 μm. (c) For co-localization imaging with mitochondrial markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were first stained with MitoTracker (red) for 2 hr at 37°C, then fixed, permeabilized, and stained with anti-CB 2 mAb (green) for 30 min at 4°C. Cells were fixed, mounted and imaged in 10–20 sections with an SP5 blue confocal microscope (top) . For co-localization with ER markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were fixed, permeabilized, and stained with anti-CB 2 mAb (green) and Concanavalin A (red) for 60 min at 4°C. Cells were immediately mounted without fixation and imaged in 10–20 sections with an SP5 blue confocal microscope (bottom) . Magnification 63X; Scale bar 25 μm.
    Figure Legend Snippet: Intracellular CB 2 is expressed in a diffuse but punctate pattern and demonstrates co-localization with endoplasmic reticulum compartments (a) CD20 + /CD3 − B cells purified from peripheral blood, cells prepared from the SUDHL-4 lymphoma cell line, and 293T/CB 2 -GFP cells were fixed, permeabilized, and stained with an Alexa Fluor® 647-conjugated mAb against CB 2 protein (displayed as magenta) and mounted with SlowFade® Diamond Antifade Mountant with DAPI (blue) prior to examination by confocal fluorescence microscopy. Magnification 63X; Scale Bar 25 μm;10–20 sections/cell with an SP5 blue confocal microscope. (b) 293T/CB 2 -GFP cells were grown on poly-l-lysine coated coverslips, fixed, permeabilized and stained with either Concanavalin A (ER, red), MitoTracker (mitochondria, red), or LysoTracker (lysosome, red) prior to mounting with SlowFade® Diamond Antifade Mountant with DAPI (blue). CB 2 and mitochondrial stained cells were fixed with 1% paraformaldehyde for 20 min at 4°C and later imaged. ER and lysosomal stained cells were imaged immediately without fixation. Cells were imaged in 10–20 sections with an SP5 blue confocal microscope. Magnification 63X; Scale Bar 25 μm. (c) For co-localization imaging with mitochondrial markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were first stained with MitoTracker (red) for 2 hr at 37°C, then fixed, permeabilized, and stained with anti-CB 2 mAb (green) for 30 min at 4°C. Cells were fixed, mounted and imaged in 10–20 sections with an SP5 blue confocal microscope (top) . For co-localization with ER markers, 293T-CB 2 -GFP cells grown on poly-l-lysine coated coverslips were fixed, permeabilized, and stained with anti-CB 2 mAb (green) and Concanavalin A (red) for 60 min at 4°C. Cells were immediately mounted without fixation and imaged in 10–20 sections with an SP5 blue confocal microscope (bottom) . Magnification 63X; Scale bar 25 μm.

    Techniques Used: Purification, Staining, Fluorescence, Microscopy, Imaging

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    Staining:

    Article Title: Theranostic Sorafenib-Loaded Polymeric Nanocarriers Manufactured by Enhanced Gadolinium Conjugation Techniques
    Article Snippet: To further analyze cellular uptake and intracellular localization, fluorescence microscopy of HepG2 cells plated on cover slides and incubated with either Cy5-labeled PLGA or PEG-PLGA NP for 4 h and 24 h was employed. .. Membrane staining was performed by using Alexa488 concanavalin A (2.5 µg/mL; Thermo Fisher Scientific, Schwerte, Germany) and nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) solution (Merck, Darmstadt, Germany). .. Finally, slides were mounted with Vectashield mounting medium (Biozol, Eching, Germany) and images were obtained using an AxioImager Z1 microscope and Axiovision 4.6 software (Carl Zeiss, Jena, Germany).

    Article Title: An In Vitro Model for Oral Mixed Biofilms of Candida albicans and Streptococcus gordonii in Synthetic Saliva
    Article Snippet: Confocal Scanning Laser Microscopy Biofilms were grown using 6-well plates (same as for SEM) and incubated in a 5% CO2 incubator for 24 h at 37°C. .. For confocal scanning laser microscopy (CSLM), biofilms were stained in the dark in the following order: at 37°C for 30 min with 25 μg/ml concavalin A-Alexa Fluor® 488 conjugate (Molecular Probes, Eugene, OR, USA), at room temperature for 30 min with 1X FilmTracerTM SYPRO® Ruby Biofilm Matrix Stain (Molecular Probes), and for 10 min at 37°C with 300 nM 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Molecular Probes). .. After incubation the supernatant was removed and the biofilms rinsed with 2 ml of PBS to remove non-adhered cells.

    Article Title: An In Vitro Model for Candida albicans–Streptococcus gordonii Biofilms on Titanium Surfaces
    Article Snippet: Samples were coated with a 60:40 gold–palladium alloy using a sputter coater and visualized using a JEOL JSM-6610 Scanning Electron Microscope (JEOL USA, Inc., Peabody, MA, USA). .. Biofilms on titanium were stained in a sequential order starting with 25 μg/mL Concanavalin A–Alexa Fluor® 488 conjugate (excitation/emission; 495/519) (Molecular Probes, Eugene, OR, USA) for 30 min at 37 °C, followed by 1 x FilmTracer™ SYPRO® Ruby Biofilm Matrix Stain (Molecular Probes) for 30 min at room temperature, and finally with 300 nM 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) (excitation/emission; 358/461) (Molecular Probes, Eugene, OR, USA) for 10 min at 37 °C. ..

    Incubation:

    Article Title: Expression Patterns and miRNA Regulation of DNA Methyltransferases in Chicken Primordial Germ Cells
    Article Snippet: The cells were incubated with 5 meC antibody (abCAM, Cambridge, UK) and diluted in blocking buffer (1∶200) at 4°C overnight. .. After primary antibody incubation, cells were washed in 1× PBS and incubated with Alexa 488 dye-conjugated secondary antibodies (Molecular Probes, Carlsbad, CA, USA) for 1 h at room temperature in the dark. .. Finally, the cells were mounted with ProLongH Gold antifade reagent with 4′-6-diamidino-2-phenylindole (DAPI, Invitrogen) and imaged with a confocal laser microscope (Carl Zeiss, Oberkochen, Germany).

    Microscopy:

    Article Title: An In Vitro Model for Oral Mixed Biofilms of Candida albicans and Streptococcus gordonii in Synthetic Saliva
    Article Snippet: Confocal Scanning Laser Microscopy Biofilms were grown using 6-well plates (same as for SEM) and incubated in a 5% CO2 incubator for 24 h at 37°C. .. For confocal scanning laser microscopy (CSLM), biofilms were stained in the dark in the following order: at 37°C for 30 min with 25 μg/ml concavalin A-Alexa Fluor® 488 conjugate (Molecular Probes, Eugene, OR, USA), at room temperature for 30 min with 1X FilmTracerTM SYPRO® Ruby Biofilm Matrix Stain (Molecular Probes), and for 10 min at 37°C with 300 nM 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Molecular Probes). .. After incubation the supernatant was removed and the biofilms rinsed with 2 ml of PBS to remove non-adhered cells.

    Whole Genome Amplification:

    Article Title: An In Vitro Model for Candida albicans–Streptococcus gordonii Biofilms on Titanium Surfaces
    Article Snippet: In addition, biofilm formation at different time points was monitored by fluorescence microscopy. .. Briefly, biofilms formed on titanium were stained in the dark in the following order: at 37 °C for 30 min with 25 µg/mL concavalin A–Alexa Fluor® 488 conjugate (Molecular Probes, Eugene, OR, USA) to label the fungal cell wall; at 37 °C for 20 min with 5 µg/mL wheat germ agglutinin, Texas Red™-X Conjugate (WGA, Molecular Probes) to label the bacterial cell wall (although a caveat here is that WGA can also bind to chitin in the fungal cell wall); and at 37 °C for 10 min with 300 nM 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Molecular Probes) to label nucleic acids. .. These samples were visualized using a 63× objective lens in a Leica DMR fluorescent microscope (Leica Microsystems, Buffalo Grove, IL, USA).

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    Thermo Fisher concanavalin a
    CSLM images of biofilms stained with concanavalin A. Biofilms were cultured for 60 h at 37°C in Spider medium and visualized with a 40× water immersion objective, and images were reconstructed to yield views from above ( x-y  plane). (A
    Concanavalin A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    CSLM images of biofilms stained with concanavalin A. Biofilms were cultured for 60 h at 37°C in Spider medium and visualized with a 40× water immersion objective, and images were reconstructed to yield views from above ( x-y  plane). (A

    Journal:

    Article Title: Candida albicans Biofilm-Defective Mutants

    doi: 10.1128/EC.4.8.1493-1502.2005

    Figure Lengend Snippet: CSLM images of biofilms stained with concanavalin A. Biofilms were cultured for 60 h at 37°C in Spider medium and visualized with a 40× water immersion objective, and images were reconstructed to yield views from above ( x-y plane). (A

    Article Snippet: Biofilms were stained in a 2-ml solution with either 0.2 mg/ml calcofluor white (fluorescent brightener 28, F3543; Sigma) or 50 to 100 μg/ml Alexa conjugate of concanavalin A (Alexa Fluor 594 nm, C-11253; Molecular Probes ) for 1 h in the dark and observed without washing.

    Techniques: Staining, Cell Culture

    Immunohistochemistry of the fugu swimbladder. Swimbladder sections were stained with anti-MCT1b (A), anti-GAPDH (B), and anti-MCT4b (C) antisera. (D) Control samples stained with nonimmune rabbit serum. The right panels show sections double- or triple-stained with Alexa Fluor 594-labeled concanavalin A (ConA) and Hoechst 33342.

    Journal: PLoS ONE

    Article Title: O2-Filled Swimbladder Employs Monocarboxylate Transporters for the Generation of O2 by Lactate-Induced Root Effect Hemoglobin

    doi: 10.1371/journal.pone.0034579

    Figure Lengend Snippet: Immunohistochemistry of the fugu swimbladder. Swimbladder sections were stained with anti-MCT1b (A), anti-GAPDH (B), and anti-MCT4b (C) antisera. (D) Control samples stained with nonimmune rabbit serum. The right panels show sections double- or triple-stained with Alexa Fluor 594-labeled concanavalin A (ConA) and Hoechst 33342.

    Article Snippet: Frozen sections (6 µm) were prepared, permeabilized with 0.2% Triton X-100 in PBS at room temperature for 10 min, and incubated with 5% FBS (Invitrogen) in PBS at room temperature for 1 h. After blocking, the sections were incubated with anti-fMCT1b antiserum (1∶1,000), anti-fMCT4b antiserum (1∶5,000), and anti-GAPDH antiserum (1∶100, Sigma) in PBS containing 5% FBS at 20°C for 16 h. After washing with PBS, the sections were incubated with a mixture of Alexa Fluor 488-labeled secondary antibodies (1∶2,000), Alexa Fluor 594-labeled ConA (50 µg/ml; Invitrogen), and Hoechst 33342 (100 ng/ml) in PBS containing 5% FBS at 20°C for 1 h. The sections were mounted on antifade glycerol (90% glycerol, 10% 10× PBS, and 0.1% 1,4-phenylenediamine at pH 7.4).

    Techniques: Immunohistochemistry, Staining, Labeling

    Subcellular localization of MCT1b and MCT4b in the fugu swimbladder. A, High-magnification images of immunohistochemical sections of the rete mirabile stained with anti-MCT1b antiserum. The right panels show sections triple-stained with Hoechst 33342 and/or Alexa Fluor 594-labeled ConA and/or fluorescently labeled phalloidin. A, arterial capillary; V, venous capillary. B, Arterial or venous small blood vessels near the rete mirabile were stained with anti-MCT1b antiserum, Hoechst 33342, and fluorescently labeled phalloidin. A, artery; V, vein. C, High-magnification images of immunohistochemical sections of the gas gland stained with anti-MCT4b antiserum. The right panels show sections triple-stained with Alexa Fluor 594-labeled ConA and Hoechst 33342. E, endothelial cell; G, gas gland cell.

    Journal: PLoS ONE

    Article Title: O2-Filled Swimbladder Employs Monocarboxylate Transporters for the Generation of O2 by Lactate-Induced Root Effect Hemoglobin

    doi: 10.1371/journal.pone.0034579

    Figure Lengend Snippet: Subcellular localization of MCT1b and MCT4b in the fugu swimbladder. A, High-magnification images of immunohistochemical sections of the rete mirabile stained with anti-MCT1b antiserum. The right panels show sections triple-stained with Hoechst 33342 and/or Alexa Fluor 594-labeled ConA and/or fluorescently labeled phalloidin. A, arterial capillary; V, venous capillary. B, Arterial or venous small blood vessels near the rete mirabile were stained with anti-MCT1b antiserum, Hoechst 33342, and fluorescently labeled phalloidin. A, artery; V, vein. C, High-magnification images of immunohistochemical sections of the gas gland stained with anti-MCT4b antiserum. The right panels show sections triple-stained with Alexa Fluor 594-labeled ConA and Hoechst 33342. E, endothelial cell; G, gas gland cell.

    Article Snippet: Frozen sections (6 µm) were prepared, permeabilized with 0.2% Triton X-100 in PBS at room temperature for 10 min, and incubated with 5% FBS (Invitrogen) in PBS at room temperature for 1 h. After blocking, the sections were incubated with anti-fMCT1b antiserum (1∶1,000), anti-fMCT4b antiserum (1∶5,000), and anti-GAPDH antiserum (1∶100, Sigma) in PBS containing 5% FBS at 20°C for 16 h. After washing with PBS, the sections were incubated with a mixture of Alexa Fluor 488-labeled secondary antibodies (1∶2,000), Alexa Fluor 594-labeled ConA (50 µg/ml; Invitrogen), and Hoechst 33342 (100 ng/ml) in PBS containing 5% FBS at 20°C for 1 h. The sections were mounted on antifade glycerol (90% glycerol, 10% 10× PBS, and 0.1% 1,4-phenylenediamine at pH 7.4).

    Techniques: Immunohistochemistry, Staining, Labeling

    The effect of R164Q mutation on the signal transduction and cell surface expression of PKR2. A , R164Q mutation disrupted the signal transduction of PKR2 as assayed with an aequorin-based assay. B , both WT and R164Q-PKR2 were presented on the cell surface. The Alexa Fluor 594-conjugated concanavalin A was used to label the membrane. The fluorescence was monitored with a confocal microscope. Scale bar: 10 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Disease-causing Mutation in PKR2 Receptor Reveals a Critical Role of Positive Charges in the Second Intracellular Loop for G-protein Coupling and Receptor Trafficking

    doi: 10.1074/jbc.M111.223784

    Figure Lengend Snippet: The effect of R164Q mutation on the signal transduction and cell surface expression of PKR2. A , R164Q mutation disrupted the signal transduction of PKR2 as assayed with an aequorin-based assay. B , both WT and R164Q-PKR2 were presented on the cell surface. The Alexa Fluor 594-conjugated concanavalin A was used to label the membrane. The fluorescence was monitored with a confocal microscope. Scale bar: 10 μm.

    Article Snippet: Alexa Fluor 594-conjugated concanavalin A (Invitrogen) was applied to label the membrane.

    Techniques: Mutagenesis, Transduction, Expressing, Fluorescence, Microscopy

    Cellular localization of FITC-labeled siRNA (green) following magnetofection (MF; A, B) and lipofection (L2000; C, D) procedures in primary fibroblasts imaged by confocal laser scanning microscopy. Both a siRNA-only control in the absence of transfection reagent (A, 2 μg siRNA; C, 1 μg siRNA) and siRNA/transfection reagent complexes (B, D) were applied. Nuclear DNA was counterstained with DAPI (blue). The cell surface marker, ConA Alexa Fluor 594 (orange-red), was used to stain the cell membrane. The last column (“merge”) shows images from the first three columns superimposed, illustrating intracellular uptake of siRNA/transfection reagent complexes (B, D) and absence of cellular uptake of FITC-siRNA alone (A, C). Co-localization of FITC-siRNA-only or siRNA/transfection reagent complexes with the cell surface did not occur.

    Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission

    Article Title: Efficient and gentle siRNA delivery by magnetofection

    doi: 10.3109/10520291003675485

    Figure Lengend Snippet: Cellular localization of FITC-labeled siRNA (green) following magnetofection (MF; A, B) and lipofection (L2000; C, D) procedures in primary fibroblasts imaged by confocal laser scanning microscopy. Both a siRNA-only control in the absence of transfection reagent (A, 2 μg siRNA; C, 1 μg siRNA) and siRNA/transfection reagent complexes (B, D) were applied. Nuclear DNA was counterstained with DAPI (blue). The cell surface marker, ConA Alexa Fluor 594 (orange-red), was used to stain the cell membrane. The last column (“merge”) shows images from the first three columns superimposed, illustrating intracellular uptake of siRNA/transfection reagent complexes (B, D) and absence of cellular uptake of FITC-siRNA alone (A, C). Co-localization of FITC-siRNA-only or siRNA/transfection reagent complexes with the cell surface did not occur.

    Article Snippet: This was followed by a direct immunostaining protocol using concanavalin A Alexa Fluor 594 conjugate for cell surface labeling (Invitrogen).

    Techniques: Labeling, Magnetofection, Confocal Laser Scanning Microscopy, Transfection, Marker, Staining