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Seikagaku concanavalin a
Illustration of main pathways to skin response via mast cells based on results shown in   Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.
Concanavalin A, supplied by Seikagaku, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation"

Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation

Journal: Experimental Animals

doi: 10.1538/expanim.15-0004

Illustration of main pathways to skin response via mast cells based on results shown in   Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.
Figure Legend Snippet: Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.

Techniques Used:

Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P
Figure Legend Snippet: Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P

Techniques Used: Permeability, Injection

2) Product Images from "Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation"

Article Title: Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation

Journal: Scientific Reports

doi: 10.1038/s41598-017-11911-9

Comparison of N-glycan profiles in HepG2 cells treated with and without 2FF. ( A ) MS spectrum of the permethylated glycans from the cells treated with DMSO only (upper) and DMSO containing 2FF (lower). ( B ) Percentages of fucosylated complex glycans in total complex glycans from HepG2 cells. The values were calculated from peak areas in the range of m/z 1,500-3,500 of the MS spectra. ( C ) HepG2 cells were cultured with 2FF for 3 days at different concentrations as indicated, and then equal amounts of cell lysates were probed with Con A, which specifically recognizes alpha-linked mannose and glucose; WGA, which selectively binds to N-Acetyl glucosamine (GlcNAc) and SNA, which specifically recognizes α 2, 6 sialylation. GAPDH was used as a loading control.
Figure Legend Snippet: Comparison of N-glycan profiles in HepG2 cells treated with and without 2FF. ( A ) MS spectrum of the permethylated glycans from the cells treated with DMSO only (upper) and DMSO containing 2FF (lower). ( B ) Percentages of fucosylated complex glycans in total complex glycans from HepG2 cells. The values were calculated from peak areas in the range of m/z 1,500-3,500 of the MS spectra. ( C ) HepG2 cells were cultured with 2FF for 3 days at different concentrations as indicated, and then equal amounts of cell lysates were probed with Con A, which specifically recognizes alpha-linked mannose and glucose; WGA, which selectively binds to N-Acetyl glucosamine (GlcNAc) and SNA, which specifically recognizes α 2, 6 sialylation. GAPDH was used as a loading control.

Techniques Used: Mass Spectrometry, Cell Culture, Whole Genome Amplification

3) Product Images from "Peripheral Lymphocyte Response to Mycophenolic Acid In Vitro and Incidence of Cytomegalovirus Infection in Renal Transplantation"

Article Title: Peripheral Lymphocyte Response to Mycophenolic Acid In Vitro and Incidence of Cytomegalovirus Infection in Renal Transplantation

Journal: Cell Medicine

doi: 10.3727/215517913X674216

Dose–response curve of MPA on PBMCs at different time points from transplantation. Typical dose–response curves of the effect of mycophenolic acid (MPA) on concanavalin A-stimulated proliferation of peripheral blood mononuclear cells (PBMCs)
Figure Legend Snippet: Dose–response curve of MPA on PBMCs at different time points from transplantation. Typical dose–response curves of the effect of mycophenolic acid (MPA) on concanavalin A-stimulated proliferation of peripheral blood mononuclear cells (PBMCs)

Techniques Used: Transplantation Assay

4) Product Images from "A Monoclonal Antibody to the ?2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model"

Article Title: A Monoclonal Antibody to the ?2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model

Journal: The Journal of Experimental Medicine

doi: 10.1084/jem.20021301

Therapeutic effects of mAb RE2 on Con A–induced hepatitis in mice. (A) Serum levels of GOT and GPT in C57BL/6 mice ( n  = 6) 24 h after an intravenous injection of Con A, with or without administration of mAb RE2. Closed and open bars represent serum levels of GOT and GPT, respectively. (B) Histopathology of massive liver cell necrosis seen in a mouse 24 h after an intravenous injection of Con A. (C) Absence of Con A-induced liver cell necrosis in a mouse treated with mAb RE2. (D) Reduction of CD69-positive activated CD4 +  and CD8 +  T cells and NK1.1 +  CD3 +  NKT cells in the liver of mice given Con A and mAb RE2 (bottom), compared with findings in mice given Con A alone (top). NKT cells were examined by triple staining of cells with mAbs to CD69, NK1.1, and CD3, and gated CD3 +  cells were analyzed for CD69 and NK1.1, using FACStar PLUS™ .
Figure Legend Snippet: Therapeutic effects of mAb RE2 on Con A–induced hepatitis in mice. (A) Serum levels of GOT and GPT in C57BL/6 mice ( n = 6) 24 h after an intravenous injection of Con A, with or without administration of mAb RE2. Closed and open bars represent serum levels of GOT and GPT, respectively. (B) Histopathology of massive liver cell necrosis seen in a mouse 24 h after an intravenous injection of Con A. (C) Absence of Con A-induced liver cell necrosis in a mouse treated with mAb RE2. (D) Reduction of CD69-positive activated CD4 + and CD8 + T cells and NK1.1 + CD3 + NKT cells in the liver of mice given Con A and mAb RE2 (bottom), compared with findings in mice given Con A alone (top). NKT cells were examined by triple staining of cells with mAbs to CD69, NK1.1, and CD3, and gated CD3 + cells were analyzed for CD69 and NK1.1, using FACStar PLUS™ .

Techniques Used: Mouse Assay, Injection, Histopathology, Staining

(A) Effects of potential inhibitors on cytotoxic activity of mAb RE2 to T cell clone MS-S2 cells. Inhibitors were added 1 or 2 h before the cytotoxicity assay. Percentages of dead cells were determined by trypan blue dye exclusion. (B) Cytotoxic sensitivity to mAb RE2 of Con A–activated splenic cells from various mouse strains. Splenic cells were first activated with 2 μg/ml of Con A for 24 h at 37°C, and then incubated with given concentrations of mAb RE2 for 1 h in the absence of complement.
Figure Legend Snippet: (A) Effects of potential inhibitors on cytotoxic activity of mAb RE2 to T cell clone MS-S2 cells. Inhibitors were added 1 or 2 h before the cytotoxicity assay. Percentages of dead cells were determined by trypan blue dye exclusion. (B) Cytotoxic sensitivity to mAb RE2 of Con A–activated splenic cells from various mouse strains. Splenic cells were first activated with 2 μg/ml of Con A for 24 h at 37°C, and then incubated with given concentrations of mAb RE2 for 1 h in the absence of complement.

Techniques Used: Activity Assay, Mass Spectrometry, Cytotoxicity Assay, Incubation

5) Product Images from "Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo"

Article Title: Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

Journal: Cytotechnology

doi: 10.1007/s10616-013-9539-5

Effect of oral administration of BFE on gene expression levels in lymphocytes. Splenocytes and PP lymphocytes from BFE-administered mice were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and cultured for 48 h.
Figure Legend Snippet: Effect of oral administration of BFE on gene expression levels in lymphocytes. Splenocytes and PP lymphocytes from BFE-administered mice were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and cultured for 48 h.

Techniques Used: Expressing, Mouse Assay, Cell Culture

Effect of BFE on cytokine production by mouse primary splenocytes in vitro. Mouse splenocytes were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and various concentrations of BFE and cultured for 48 h.
Figure Legend Snippet: Effect of BFE on cytokine production by mouse primary splenocytes in vitro. Mouse splenocytes were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and various concentrations of BFE and cultured for 48 h.

Techniques Used: In Vitro, Cell Culture

Related Articles

other:

Article Title: ST3GAL3, ST3GAL4, and ST3GAL6 differ in their regulation of biological functions via the specificities for the α2,3-sialylation of target proteins.
Article Snippet: The peroxidase-conjugated secondary antibody against rabbit (#7074S) was obtained from Cell Signaling Technology; peroxidaseconjugated secondary antibodies against mouse (#AP124P) were from MilliporeSigma; Maackia amurensis (MAM) lectin (#BA-s7801-2) was from EY Laboratories, Inc; Concanavalin A (ConA) lectin (#B-1005) from Seikagaku Corporation; MAM-Agarose (J310) and SSA-Agarose (J318) were from J-OIL MILLS, Inc An ABC kit was acquired from Vector Laboratories; doxycycline hyclate (DOX) (#D9891) was from Sigma-Aldrich; PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (#RR047A) was from Takara, Japan; Quick Taq HS DyeMix (DTM-101) was from TOYOBO, Japan.

SDS Page:

Article Title: Expression of N-Acetylglucosaminyltransferase III Suppresses α2,3-Sialylation, and Its Distinctive Functions in Cell Migration Are Attributed to α2,6-Sialylation Levels *
Article Snippet: Insoluble materials were removed by centrifugation at 15,000 rpm for 10 min at 4 °C. .. Equal amounts of protein were separated using 7.5% SDS-PAGE, transferred to PVDF, and probed with the appropriate antibodies as indicated or with biotinylated erythro-agglutinating phytohemagglutinin (E4-PHA), biotinylated Sambucus nigra lectin (SNA), Maackia amurensis agglutinin (MAA), Datura stramonium agglutinin (DSA), and concanavalin A (ConA) lectins (Seikagaku Kogyo Inc., Tokyo, Japan). .. Immunoreactive bands were visualized using a Vectastain ABC kit (Vector Laboratories) and an ECL kit (Amersham Biosciences).

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    Seikagaku concanavalin a
    Comparison of N-glycan profiles in HepG2 cells treated with and without 2FF. ( A ) MS spectrum of the permethylated glycans from the cells treated with DMSO only (upper) and DMSO containing 2FF (lower). ( B ) Percentages of fucosylated complex glycans in total complex glycans from HepG2 cells. The values were calculated from peak areas in the range of m/z 1,500-3,500 of the MS spectra. ( C ) HepG2 cells were cultured with 2FF for 3 days at different concentrations as indicated, and then equal amounts of cell lysates were probed with Con A, which specifically recognizes alpha-linked mannose and glucose; WGA, which selectively binds to N-Acetyl glucosamine (GlcNAc) and SNA, which specifically recognizes α 2, 6 sialylation. GAPDH was used as a loading control.
    Concanavalin A, supplied by Seikagaku, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concanavalin a/product/Seikagaku
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a - by Bioz Stars, 2021-03
    86/100 stars
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    Comparison of N-glycan profiles in HepG2 cells treated with and without 2FF. ( A ) MS spectrum of the permethylated glycans from the cells treated with DMSO only (upper) and DMSO containing 2FF (lower). ( B ) Percentages of fucosylated complex glycans in total complex glycans from HepG2 cells. The values were calculated from peak areas in the range of m/z 1,500-3,500 of the MS spectra. ( C ) HepG2 cells were cultured with 2FF for 3 days at different concentrations as indicated, and then equal amounts of cell lysates were probed with Con A, which specifically recognizes alpha-linked mannose and glucose; WGA, which selectively binds to N-Acetyl glucosamine (GlcNAc) and SNA, which specifically recognizes α 2, 6 sialylation. GAPDH was used as a loading control.

    Journal: Scientific Reports

    Article Title: Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation

    doi: 10.1038/s41598-017-11911-9

    Figure Lengend Snippet: Comparison of N-glycan profiles in HepG2 cells treated with and without 2FF. ( A ) MS spectrum of the permethylated glycans from the cells treated with DMSO only (upper) and DMSO containing 2FF (lower). ( B ) Percentages of fucosylated complex glycans in total complex glycans from HepG2 cells. The values were calculated from peak areas in the range of m/z 1,500-3,500 of the MS spectra. ( C ) HepG2 cells were cultured with 2FF for 3 days at different concentrations as indicated, and then equal amounts of cell lysates were probed with Con A, which specifically recognizes alpha-linked mannose and glucose; WGA, which selectively binds to N-Acetyl glucosamine (GlcNAc) and SNA, which specifically recognizes α 2, 6 sialylation. GAPDH was used as a loading control.

    Article Snippet: Biotinylated aleuria aurantia lectin (AAL), concanavalin A (ConA) and wheat germ agglutinin (WGA) were purchased from Seikagaku Crop (Tokyo, Japan).

    Techniques: Mass Spectrometry, Cell Culture, Whole Genome Amplification

    Effect of oral administration of BFE on gene expression levels in lymphocytes. Splenocytes and PP lymphocytes from BFE-administered mice were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and cultured for 48 h.

    Journal: Cytotechnology

    Article Title: Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

    doi: 10.1007/s10616-013-9539-5

    Figure Lengend Snippet: Effect of oral administration of BFE on gene expression levels in lymphocytes. Splenocytes and PP lymphocytes from BFE-administered mice were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and cultured for 48 h.

    Article Snippet: To examine the effect of BFE on cytokine production, splenocytes were inoculated into a 48-well culture plate at 2.0 × 106 cells/mL in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of concanavalin A (Con A; Seikagaku, Tokyo, Japan) and cultured for 48 h. The amounts of interleukin (IL)-4, IL-5, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ in culture medium were measured by commercially available ELISA kits (eBioscience, San Diego, CA, USA).

    Techniques: Expressing, Mouse Assay, Cell Culture

    Effect of BFE on cytokine production by mouse primary splenocytes in vitro. Mouse splenocytes were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and various concentrations of BFE and cultured for 48 h.

    Journal: Cytotechnology

    Article Title: Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

    doi: 10.1007/s10616-013-9539-5

    Figure Lengend Snippet: Effect of BFE on cytokine production by mouse primary splenocytes in vitro. Mouse splenocytes were inoculated in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of Con A and various concentrations of BFE and cultured for 48 h.

    Article Snippet: To examine the effect of BFE on cytokine production, splenocytes were inoculated into a 48-well culture plate at 2.0 × 106 cells/mL in 5 % FBS-RPMI 1640 medium containing 5 μg/mL of concanavalin A (Con A; Seikagaku, Tokyo, Japan) and cultured for 48 h. The amounts of interleukin (IL)-4, IL-5, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ in culture medium were measured by commercially available ELISA kits (eBioscience, San Diego, CA, USA).

    Techniques: In Vitro, Cell Culture

    Dose–response curve of MPA on PBMCs at different time points from transplantation. Typical dose–response curves of the effect of mycophenolic acid (MPA) on concanavalin A-stimulated proliferation of peripheral blood mononuclear cells (PBMCs)

    Journal: Cell Medicine

    Article Title: Peripheral Lymphocyte Response to Mycophenolic Acid In Vitro and Incidence of Cytomegalovirus Infection in Renal Transplantation

    doi: 10.3727/215517913X674216

    Figure Lengend Snippet: Dose–response curve of MPA on PBMCs at different time points from transplantation. Typical dose–response curves of the effect of mycophenolic acid (MPA) on concanavalin A-stimulated proliferation of peripheral blood mononuclear cells (PBMCs)

    Article Snippet: Concanavalin A was obtained from Seikagaku Kogyo Co. (Tokyo, Japan).

    Techniques: Transplantation Assay

    Therapeutic effects of mAb RE2 on Con A–induced hepatitis in mice. (A) Serum levels of GOT and GPT in C57BL/6 mice ( n  = 6) 24 h after an intravenous injection of Con A, with or without administration of mAb RE2. Closed and open bars represent serum levels of GOT and GPT, respectively. (B) Histopathology of massive liver cell necrosis seen in a mouse 24 h after an intravenous injection of Con A. (C) Absence of Con A-induced liver cell necrosis in a mouse treated with mAb RE2. (D) Reduction of CD69-positive activated CD4 +  and CD8 +  T cells and NK1.1 +  CD3 +  NKT cells in the liver of mice given Con A and mAb RE2 (bottom), compared with findings in mice given Con A alone (top). NKT cells were examined by triple staining of cells with mAbs to CD69, NK1.1, and CD3, and gated CD3 +  cells were analyzed for CD69 and NK1.1, using FACStar PLUS™ .

    Journal: The Journal of Experimental Medicine

    Article Title: A Monoclonal Antibody to the ?2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model

    doi: 10.1084/jem.20021301

    Figure Lengend Snippet: Therapeutic effects of mAb RE2 on Con A–induced hepatitis in mice. (A) Serum levels of GOT and GPT in C57BL/6 mice ( n = 6) 24 h after an intravenous injection of Con A, with or without administration of mAb RE2. Closed and open bars represent serum levels of GOT and GPT, respectively. (B) Histopathology of massive liver cell necrosis seen in a mouse 24 h after an intravenous injection of Con A. (C) Absence of Con A-induced liver cell necrosis in a mouse treated with mAb RE2. (D) Reduction of CD69-positive activated CD4 + and CD8 + T cells and NK1.1 + CD3 + NKT cells in the liver of mice given Con A and mAb RE2 (bottom), compared with findings in mice given Con A alone (top). NKT cells were examined by triple staining of cells with mAbs to CD69, NK1.1, and CD3, and gated CD3 + cells were analyzed for CD69 and NK1.1, using FACStar PLUS™ .

    Article Snippet: Lab., Z-VAD-fmk and Z-Asp-DCB from Peptide Institute, Inc., Concanavalin A (Con A) from Seikagaku Co. Other reagents used were purchased from Sigma-Aldrich.

    Techniques: Mouse Assay, Injection, Histopathology, Staining

    (A) Effects of potential inhibitors on cytotoxic activity of mAb RE2 to T cell clone MS-S2 cells. Inhibitors were added 1 or 2 h before the cytotoxicity assay. Percentages of dead cells were determined by trypan blue dye exclusion. (B) Cytotoxic sensitivity to mAb RE2 of Con A–activated splenic cells from various mouse strains. Splenic cells were first activated with 2 μg/ml of Con A for 24 h at 37°C, and then incubated with given concentrations of mAb RE2 for 1 h in the absence of complement.

    Journal: The Journal of Experimental Medicine

    Article Title: A Monoclonal Antibody to the ?2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model

    doi: 10.1084/jem.20021301

    Figure Lengend Snippet: (A) Effects of potential inhibitors on cytotoxic activity of mAb RE2 to T cell clone MS-S2 cells. Inhibitors were added 1 or 2 h before the cytotoxicity assay. Percentages of dead cells were determined by trypan blue dye exclusion. (B) Cytotoxic sensitivity to mAb RE2 of Con A–activated splenic cells from various mouse strains. Splenic cells were first activated with 2 μg/ml of Con A for 24 h at 37°C, and then incubated with given concentrations of mAb RE2 for 1 h in the absence of complement.

    Article Snippet: Lab., Z-VAD-fmk and Z-Asp-DCB from Peptide Institute, Inc., Concanavalin A (Con A) from Seikagaku Co. Other reagents used were purchased from Sigma-Aldrich.

    Techniques: Activity Assay, Mass Spectrometry, Cytotoxicity Assay, Incubation