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FUJIFILM concanavalin a
Effect of oral administration of the  L. pentosus  strain S-PT84 on stress-induced immune compromisation of splenocytes. Splenocytes were collected from mice that were fed an AIN-93 M diet with or without S-PT84 for 1 week and then restrained. The number of splenocytes (A), splenic NK activity against target YAC-1 cells (effector:target ratio = 80:1, 40:1, and 20:1) (B), and IFN-γ (C) and IL-4 (D) production levels in splenocytes cultured with Con A (2.5 µg/ml) for 24 hr were measured as described in MATERIALS AND METHODS. Data are presented as the mean ± SE of five mice. Different letters ( a ,  b  and  c ) indicate significant differences between groups (p
Concanavalin A, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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concanavalin a - by Bioz Stars, 2021-03
86/100 stars

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1) Product Images from "Anti-stress effect of the Lactobacillus pentosus strain S-PT84 in mice"

Article Title: Anti-stress effect of the Lactobacillus pentosus strain S-PT84 in mice

Journal: Bioscience of Microbiota, Food and Health

doi: 10.12938/bmfh.17-003

Effect of oral administration of the  L. pentosus  strain S-PT84 on stress-induced immune compromisation of splenocytes. Splenocytes were collected from mice that were fed an AIN-93 M diet with or without S-PT84 for 1 week and then restrained. The number of splenocytes (A), splenic NK activity against target YAC-1 cells (effector:target ratio = 80:1, 40:1, and 20:1) (B), and IFN-γ (C) and IL-4 (D) production levels in splenocytes cultured with Con A (2.5 µg/ml) for 24 hr were measured as described in MATERIALS AND METHODS. Data are presented as the mean ± SE of five mice. Different letters ( a ,  b  and  c ) indicate significant differences between groups (p
Figure Legend Snippet: Effect of oral administration of the L. pentosus strain S-PT84 on stress-induced immune compromisation of splenocytes. Splenocytes were collected from mice that were fed an AIN-93 M diet with or without S-PT84 for 1 week and then restrained. The number of splenocytes (A), splenic NK activity against target YAC-1 cells (effector:target ratio = 80:1, 40:1, and 20:1) (B), and IFN-γ (C) and IL-4 (D) production levels in splenocytes cultured with Con A (2.5 µg/ml) for 24 hr were measured as described in MATERIALS AND METHODS. Data are presented as the mean ± SE of five mice. Different letters ( a , b and c ) indicate significant differences between groups (p

Techniques Used: Mouse Assay, Activity Assay, Cell Culture

2) Product Images from "Protecting Group-Free Synthesis of Glycopolymer-Type Amphiphilic Macromonomers and Their Use for the Preparation of Carbohydrate-Decorated Polymer Particles"

Article Title: Protecting Group-Free Synthesis of Glycopolymer-Type Amphiphilic Macromonomers and Their Use for the Preparation of Carbohydrate-Decorated Polymer Particles

Journal: Biomolecules

doi: 10.3390/biom9020072

Transmittance of Mal-decorated polymer particle suspensions prepared using ( a ) P3 and ( b ) P4 after adding lectins for 2h. Control: polymer particle suspension before the addition of Con A and BSA.
Figure Legend Snippet: Transmittance of Mal-decorated polymer particle suspensions prepared using ( a ) P3 and ( b ) P4 after adding lectins for 2h. Control: polymer particle suspension before the addition of Con A and BSA.

Techniques Used:

3) Product Images from "A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response [S]"

Article Title: A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response [S]

Journal: Journal of Lipid Research

doi: 10.1194/jlr.M062794

Effect of CU-3 on the IL-2 production of Jurkat T cells. A: Jurkat T cells were preincubated in 35 mm culture dishes filled with 2 ml RPMI in the presence or absence of CU-3 (5 μM) for 5 min. Con A was then added to the media, and the cells were
Figure Legend Snippet: Effect of CU-3 on the IL-2 production of Jurkat T cells. A: Jurkat T cells were preincubated in 35 mm culture dishes filled with 2 ml RPMI in the presence or absence of CU-3 (5 μM) for 5 min. Con A was then added to the media, and the cells were

Techniques Used:

4) Product Images from "Development of Mannose-Modified Carboxylated Curdlan-Coated Liposomes for Antigen Presenting Cell Targeted Antigen Delivery"

Article Title: Development of Mannose-Modified Carboxylated Curdlan-Coated Liposomes for Antigen Presenting Cell Targeted Antigen Delivery

Journal: Pharmaceutics

doi: 10.3390/pharmaceutics12080754

Turbidity change at 360 nm of curdlan derivative-modified liposome suspension in the presence of Con A ( a ), Con A and free mannose ( b ) and BSA ( c ).
Figure Legend Snippet: Turbidity change at 360 nm of curdlan derivative-modified liposome suspension in the presence of Con A ( a ), Con A and free mannose ( b ) and BSA ( c ).

Techniques Used: Modification

Related Articles

Microscopy:

Article Title: Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing
Article Snippet: In brief, S. pombe cells were cultured at 26°C in EMM2 medium and were shifted to 30°C for the appropriate duration. .. Cells were transferred to a glass-bottomed dish (IWAKI Glass) coated with concanavalin A (Wako) before being examined under the microscope (DeltaVision; Applied Precision). .. Time-lapse images were recorded by three-dimensional microscopy using the DeltaVision system.

Incubation:

Article Title: Receptor-Independent Infection of Murine Coronavirus: Analysis by Spinoculation
Article Snippet: BHK and DBT cells were seeded at a concentration of 5 × 105 cells in 0.5 ml per well of a 24-well culture plate (Falcon, Franklin Lakes, NJ). .. After 6 h of incubation, cells were washed once with phosphate-buffered saline (pH 7.2) (PBS) and inoculated with viruses serially diluted by 10-fold in 300 μl of DMEM containing 0.5 μg of concanavalin A (ConA; Wako, Osaka, Japan)/ml. .. As a control, cells in 24-well plates were inoculated with virus in 50 μl of DMEM and cultured for 1 h at 4°C without centrifugation.

Article Title: A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response [S]
Article Snippet: The assay for IL-2 mRNA expression in Jurkat T cells was carried out as previously reported ( ). .. Jurkat cells were preincubated in 35 mm culture dishes filled with 2 ml of RPMI in the presence or absence of CU-3 (5 μM) for 5 min. Concanavalin A (Con A) was then added to the media, and the cells were further incubated for 3 h, collected by centrifugation (400 g , 5 min), and lysed with 1 ml of ISOGEN (Wako Pure Chemical Industries). .. Total RNA was prepared, and 1 μg of total RNA was reverse transcribed into cDNA according to the manufacturer’s instructions (for ISOGEN).

Centrifugation:

Article Title: A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response [S]
Article Snippet: The assay for IL-2 mRNA expression in Jurkat T cells was carried out as previously reported ( ). .. Jurkat cells were preincubated in 35 mm culture dishes filled with 2 ml of RPMI in the presence or absence of CU-3 (5 μM) for 5 min. Concanavalin A (Con A) was then added to the media, and the cells were further incubated for 3 h, collected by centrifugation (400 g , 5 min), and lysed with 1 ml of ISOGEN (Wako Pure Chemical Industries). .. Total RNA was prepared, and 1 μg of total RNA was reverse transcribed into cDNA according to the manufacturer’s instructions (for ISOGEN).

Cell Culture:

Article Title: Anti-stress effect of the Lactobacillus pentosus strain S-PT84 in mice
Article Snippet: Ex vivo assessment of cytokines Peritoneal macrophages (2 × 106 cells/ml) that were collected as described above and splenocytes (5 × 106 cells/ml) were each cultured in RPMI-1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% FBS in 24-well microplates (BD Biosciences). .. Macrophages were cultured with lipopolysaccharide (LPS, from Escherichia coli 055; 100 ng/ml; Wako Pure Chemical industries, Osaka, Japan) for 48 hr, and splenocytes were cultured with Concanavalin A (Con A; 2.5 µg/ml, Wako) for 24 hr at 37°C. .. The culture supernatant was collected, and IL-12 (p70) and IL-10 production by macrophages and IFN-γ and IL-4 production by splenocytes were measured by using an OptEIA ELISA kit (BD Pharmingen, San Diego, CA, USA).

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    FUJIFILM concanavalin a
    Effect of oral administration of the  L. pentosus  strain S-PT84 on stress-induced immune compromisation of splenocytes. Splenocytes were collected from mice that were fed an AIN-93 M diet with or without S-PT84 for 1 week and then restrained. The number of splenocytes (A), splenic NK activity against target YAC-1 cells (effector:target ratio = 80:1, 40:1, and 20:1) (B), and IFN-γ (C) and IL-4 (D) production levels in splenocytes cultured with Con A (2.5 µg/ml) for 24 hr were measured as described in MATERIALS AND METHODS. Data are presented as the mean ± SE of five mice. Different letters ( a ,  b  and  c ) indicate significant differences between groups (p
    Concanavalin A, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concanavalin a/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Effect of oral administration of the  L. pentosus  strain S-PT84 on stress-induced immune compromisation of splenocytes. Splenocytes were collected from mice that were fed an AIN-93 M diet with or without S-PT84 for 1 week and then restrained. The number of splenocytes (A), splenic NK activity against target YAC-1 cells (effector:target ratio = 80:1, 40:1, and 20:1) (B), and IFN-γ (C) and IL-4 (D) production levels in splenocytes cultured with Con A (2.5 µg/ml) for 24 hr were measured as described in MATERIALS AND METHODS. Data are presented as the mean ± SE of five mice. Different letters ( a ,  b  and  c ) indicate significant differences between groups (p

    Journal: Bioscience of Microbiota, Food and Health

    Article Title: Anti-stress effect of the Lactobacillus pentosus strain S-PT84 in mice

    doi: 10.12938/bmfh.17-003

    Figure Lengend Snippet: Effect of oral administration of the L. pentosus strain S-PT84 on stress-induced immune compromisation of splenocytes. Splenocytes were collected from mice that were fed an AIN-93 M diet with or without S-PT84 for 1 week and then restrained. The number of splenocytes (A), splenic NK activity against target YAC-1 cells (effector:target ratio = 80:1, 40:1, and 20:1) (B), and IFN-γ (C) and IL-4 (D) production levels in splenocytes cultured with Con A (2.5 µg/ml) for 24 hr were measured as described in MATERIALS AND METHODS. Data are presented as the mean ± SE of five mice. Different letters ( a , b and c ) indicate significant differences between groups (p

    Article Snippet: Macrophages were cultured with lipopolysaccharide (LPS, from Escherichia coli 055; 100 ng/ml; Wako Pure Chemical industries, Osaka, Japan) for 48 hr, and splenocytes were cultured with Concanavalin A (Con A; 2.5 µg/ml, Wako) for 24 hr at 37°C.

    Techniques: Mouse Assay, Activity Assay, Cell Culture

    Transmittance of Mal-decorated polymer particle suspensions prepared using ( a ) P3 and ( b ) P4 after adding lectins for 2h. Control: polymer particle suspension before the addition of Con A and BSA.

    Journal: Biomolecules

    Article Title: Protecting Group-Free Synthesis of Glycopolymer-Type Amphiphilic Macromonomers and Their Use for the Preparation of Carbohydrate-Decorated Polymer Particles

    doi: 10.3390/biom9020072

    Figure Lengend Snippet: Transmittance of Mal-decorated polymer particle suspensions prepared using ( a ) P3 and ( b ) P4 after adding lectins for 2h. Control: polymer particle suspension before the addition of Con A and BSA.

    Article Snippet: Chemicals and Reagents d (+)-maltose monohydrate, sodium azide, 2-chloro-1, 3-dimethylimidazolinium chloride (DMC), copper (II) sulfate pentahydrate, l -ascorbic acid sodium salt, 2,2′-azobis-[2-(2-imidazolin-2-yl)-propane] dihydrochlroride (VA-044), styrene, concanavalin A (Con A) from Canavalia ensiformis , and bovine serum albumin (BSA) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).

    Techniques:

    Effect of CU-3 on the IL-2 production of Jurkat T cells. A: Jurkat T cells were preincubated in 35 mm culture dishes filled with 2 ml RPMI in the presence or absence of CU-3 (5 μM) for 5 min. Con A was then added to the media, and the cells were

    Journal: Journal of Lipid Research

    Article Title: A novel diacylglycerol kinase α-selective inhibitor, CU-3, induces cancer cell apoptosis and enhances immune response [S]

    doi: 10.1194/jlr.M062794

    Figure Lengend Snippet: Effect of CU-3 on the IL-2 production of Jurkat T cells. A: Jurkat T cells were preincubated in 35 mm culture dishes filled with 2 ml RPMI in the presence or absence of CU-3 (5 μM) for 5 min. Con A was then added to the media, and the cells were

    Article Snippet: Jurkat cells were preincubated in 35 mm culture dishes filled with 2 ml of RPMI in the presence or absence of CU-3 (5 μM) for 5 min. Concanavalin A (Con A) was then added to the media, and the cells were further incubated for 3 h, collected by centrifugation (400 g , 5 min), and lysed with 1 ml of ISOGEN (Wako Pure Chemical Industries).

    Techniques: