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Boehringer Mannheim concanavalin a
HIV-1 causes a profound cytopathic effect on cultured CD4 + T lymphocytes from peripheral blood. Purified CD4 + T lymphocytes were activated with <t>concanavalin</t> A and IL-2 and then infected with the NL4-3 HSA ).
Concanavalin A, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/concanavalin a/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
concanavalin a - by Bioz Stars, 2021-03
86/100 stars

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1) Product Images from "Cytopathic Killing of Peripheral Blood CD4+ T Lymphocytes by Human Immunodeficiency Virus Type 1 Appears Necrotic rather than Apoptotic and Does Not Require env"

Article Title: Cytopathic Killing of Peripheral Blood CD4+ T Lymphocytes by Human Immunodeficiency Virus Type 1 Appears Necrotic rather than Apoptotic and Does Not Require env

Journal: Journal of Virology

doi: 10.1128/JVI.76.10.5082-5093.2002

HIV-1 causes a profound cytopathic effect on cultured CD4 + T lymphocytes from peripheral blood. Purified CD4 + T lymphocytes were activated with concanavalin A and IL-2 and then infected with the NL4-3 HSA ).
Figure Legend Snippet: HIV-1 causes a profound cytopathic effect on cultured CD4 + T lymphocytes from peripheral blood. Purified CD4 + T lymphocytes were activated with concanavalin A and IL-2 and then infected with the NL4-3 HSA ).

Techniques Used: Cell Culture, Purification, Infection

Related Articles

Recombinant:

Article Title: Multiple Antiviral Activities of Cyanovirin-N: Blocking of Human Immunodeficiency Virus Type 1 gp120 Interaction with CD4 and Coreceptor and Inhibition of Diverse Enveloped Viruses
Article Snippet: Both cell lines were cultured in RPMI 1640 supplemented with 10% heat-inactivated FBS, minimal essential medium (MEM)-vitamins, nonessential amino acids, sodium pyruvate, 200 μM l -glutamine, 5.5 × 10−5 M β-mercaptoethanol, and 50 μg of gentamicin/ml. .. The MCH5-4 cells were supplemented with recombinant human interleukin 2 (50 U/ml; a generous gift of Hoffmann-La Roche, Nutley, N.J.) and concanavalin A (2.5 μg/ml; Boehringer Mannheim, Indianapolis, Ind.). .. All vaccinia virus recombinants were derived from the WR wild-type strain.

Article Title: T-Cell Epitopes and Human Leukocyte Antigen Restriction Elements of an Immunodominant Antigen of Blastomyces dermatitidis
Article Snippet: Concentrations were as follows: Candida antigen (Hollister-Stier Laboratories, Spokane, Wash.), 1:100; tetanus toxoid (Wyeth-Ayerst Laboratories, Paoli, Pa.), 1:10; BASWS, 100 μg/ml; native WI-1, 10 μg/ml; recombinant WI-1 (rWI-1), C terminus, N terminus, tandem repeat, 4D, 11, 13, N fragments, DHFR, and N fragment and tandem repeat synthetic peptides, 20 μg/ml each. .. Concanavalin A (ConA; Boehringer Mannheim, Indianapolis, Ind.) at 10 μg/ml and recombinant interleukin-2 (rIL-2) at 200 U/ml served as positive controls. ..

Cell Culture:

Article Title: Expanded Host Cell Tropism and Cytopathic Properties of Feline Immunodeficiency Virus Strain PPR Subsequent to Passage through Interleukin-2-Independent T Cells
Article Snippet: Crandell feline kidney cells (CrFK) were obtained from the American Type Culture Collection ATCC , and G355-5, a feline glial cell line, was kindly provided by Don Blair (National Institutes of Health, Bethesda, Md.). .. The T-cell lines, MCH5-4 and 104-C1, were cultured in RPMI 1640 (Mediatech) supplemented with 10% heat-inactivated fetal bovine serum (Gemini-BioProducts), 1× MEM-vitamins (Sigma, St. Louis, Mo.), 1× nonessential amino acids (Sigma), 1× sodium pyruvate (Sigma), 200 μM l -glutamine (Sigma), 5.5 × 10−5 M β-mercaptoethanol (Gibco-BRL), 50 μg of gentamicin (Gemini-BioProducts) per ml, 50 U of recombinant human IL-2 (rhIL-2) (a generous gift of Hoffmann-La Roche), and 2.5 μg of concanavalin A (Boehringer Mannheim) per ml. .. The IL-2-independent T-cell lines, as well as the CrFK and G355-5 cells, were cultured as above without the addition of IL-2 or concanavalin A.

Article Title: CD28-B7 Costimulatory Blockade by CTLA4Ig Delays the Development of Retrovirus-Induced Murine AIDS
Article Snippet: .. Aliquots containing 2 × 105 LN or SP lymphocytes from individual healthy and experimental mice were cultured in triplicate in 96-well microtest plates for 72 h with concanavalin A (ConA; Boehringer, Mannheim, Germany) at 5 μg/ml or with lipopolysaccharide (LPS; Difco, Detroit, Mich.) at 10 μg/ml. .. During the last 4 h of culture, cells were incubated with 0.4 μCi of [3 H]thymidine (6.7 Ci/mmol) (Dupont, NEN Products, Boston, Mass.) and collected with a cell harvester (Skatron, Sterling, Va.) onto glass fiber filters.

Positive Control:

Article Title: Fate of Mycobacterium tuberculosis within Murine Dendritic Cells
Article Snippet: .. As a positive control, cells were stimulated with concanavalin A (ConA; Boehringer Mannheim Corp., Indianapolis, Ind.) at 5 μg/ml. .. Cells were pulsed for the final 12 to 18 h of culture with 1 μCi of [3 H]thymidine (Amersham Life Sciences, Inc., Arlington Heights, Ill.) per well, and the incorporation of radioactivity was measured by counting cell lysates on filters in a scintillation counter.

Mouse Assay:

Article Title: CD28-B7 Costimulatory Blockade by CTLA4Ig Delays the Development of Retrovirus-Induced Murine AIDS
Article Snippet: .. Aliquots containing 2 × 105 LN or SP lymphocytes from individual healthy and experimental mice were cultured in triplicate in 96-well microtest plates for 72 h with concanavalin A (ConA; Boehringer, Mannheim, Germany) at 5 μg/ml or with lipopolysaccharide (LPS; Difco, Detroit, Mich.) at 10 μg/ml. .. During the last 4 h of culture, cells were incubated with 0.4 μCi of [3 H]thymidine (6.7 Ci/mmol) (Dupont, NEN Products, Boston, Mass.) and collected with a cell harvester (Skatron, Sterling, Va.) onto glass fiber filters.

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    Boehringer Mannheim biotinylated concanavalin a
    Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using <t>biotinylated</t> <t>concanavalin</t> A (ConA). Bar = 15 μm.
    Biotinylated Concanavalin A, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated concanavalin a/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated concanavalin a - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Boehringer Mannheim concanavalin a
    Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using <t>biotinylated</t> <t>concanavalin</t> A (ConA). Bar = 15 μm.
    Concanavalin A, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concanavalin a/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concanavalin a - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.

    Journal: Molecular Biology of the Cell

    Article Title: Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases

    doi:

    Figure Lengend Snippet: Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.

    Article Snippet: The coverslips were treated with different first antibodies, such as anti-FLAG (Sigma, St. Louis, MO), anti-vesicular stomatitis virus (VSV) (Sigma), and anti-human placental alkaline phosphatase (hPLAP), or with biotinylated concanavalin A (Boehringer, Mannheim, Germany) diluted in PBS, and with 3% BSA for 1 h in a moist chamber at 37°C.

    Techniques: Staining

    Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.

    Journal: Molecular Biology of the Cell

    Article Title: Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases

    doi:

    Figure Lengend Snippet: Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.

    Article Snippet: The coverslips were treated with different first antibodies, such as anti-FLAG (Sigma, St. Louis, MO), anti-vesicular stomatitis virus (VSV) (Sigma), and anti-human placental alkaline phosphatase (hPLAP), or with biotinylated concanavalin A (Boehringer, Mannheim, Germany) diluted in PBS, and with 3% BSA for 1 h in a moist chamber at 37°C.

    Techniques: Sequencing, Staining, Transfection, Construct