concanavalin a fitc  (Millipore)

 
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    Name:
    Fluorescein isothiocyanate
    Description:

    Catalog Number:
    46947
    Price:
    None
    Applications:
    Fluorescein isothiocyanate - Dextran 500000-Conjugate is used as a fluorescent tissue permeability assay reagent and as a diffusion probe for evaluation of biomaterials such as hydrogels and lipid vesicles.
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    Structured Review

    Millipore concanavalin a fitc
    Bipolar actin structures are displayed only in diploid virgin daughter cells budding at the distal pole in the psi1 Δ/ psi1 Δ and bud9 Δ/ bud9 Δ mutants, and PSI1 overexpression suppresses this phenotype. (A) Cells were grown to log phase in YPD at 30°C, labeled with concanavalin <t>A-FITC</t> (birth scars), fixed, and stained with Alexa Fluor 568-phalloidin (actin) and, finally, with calcofluor white (bud scars), as described in Materials and Methods. Arrows, some cells with bipolar actin patches. Bars, 4 μm. (B) Cells were grown to an OD 600 of 0.15 in SC medium without uracil at 30°C, shifted to YPD, grown to an OD 600 of 0.6 (the rate of plasmid loss was quantified to range from 1.4% up to 3.7% per generation), and then fixed and stained with Alexa Fluor 568-phalloidin. Diploid cells with bipolarized actin cortical patches were visualized and scored among cells with small buds. *, P

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    Images

    1) Product Images from "Requirement of Phosphoinositides Containing Stearic Acid To Control Cell Polarity"

    Article Title: Requirement of Phosphoinositides Containing Stearic Acid To Control Cell Polarity

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.00843-15

    Bipolar actin structures are displayed only in diploid virgin daughter cells budding at the distal pole in the psi1 Δ/ psi1 Δ and bud9 Δ/ bud9 Δ mutants, and PSI1 overexpression suppresses this phenotype. (A) Cells were grown to log phase in YPD at 30°C, labeled with concanavalin A-FITC (birth scars), fixed, and stained with Alexa Fluor 568-phalloidin (actin) and, finally, with calcofluor white (bud scars), as described in Materials and Methods. Arrows, some cells with bipolar actin patches. Bars, 4 μm. (B) Cells were grown to an OD 600 of 0.15 in SC medium without uracil at 30°C, shifted to YPD, grown to an OD 600 of 0.6 (the rate of plasmid loss was quantified to range from 1.4% up to 3.7% per generation), and then fixed and stained with Alexa Fluor 568-phalloidin. Diploid cells with bipolarized actin cortical patches were visualized and scored among cells with small buds. *, P
    Figure Legend Snippet: Bipolar actin structures are displayed only in diploid virgin daughter cells budding at the distal pole in the psi1 Δ/ psi1 Δ and bud9 Δ/ bud9 Δ mutants, and PSI1 overexpression suppresses this phenotype. (A) Cells were grown to log phase in YPD at 30°C, labeled with concanavalin A-FITC (birth scars), fixed, and stained with Alexa Fluor 568-phalloidin (actin) and, finally, with calcofluor white (bud scars), as described in Materials and Methods. Arrows, some cells with bipolar actin patches. Bars, 4 μm. (B) Cells were grown to an OD 600 of 0.15 in SC medium without uracil at 30°C, shifted to YPD, grown to an OD 600 of 0.6 (the rate of plasmid loss was quantified to range from 1.4% up to 3.7% per generation), and then fixed and stained with Alexa Fluor 568-phalloidin. Diploid cells with bipolarized actin cortical patches were visualized and scored among cells with small buds. *, P

    Techniques Used: Over Expression, Labeling, Staining, Plasmid Preparation

    2) Product Images from "Human aquaporin-11 guarantees efficient transport of H2O2 across the endoplasmic reticulum membrane"

    Article Title: Human aquaporin-11 guarantees efficient transport of H2O2 across the endoplasmic reticulum membrane

    Journal: Redox Biology

    doi: 10.1016/j.redox.2019.101326

    AQP11 resides in the ER. A. HeLa transfectants expressing HaloAQP11 were co-stained with fluorescent Halo ligands, and with antibodies against calnexin (CNX) or peroxiredoxin 3 (PRX3), to decorate AQP11, the ER and mitochondria, respectively. To label glycoproteins on the plasma membrane, cells were stained with concanavalin A-FITC (ConA) in absence of cell permeabilization, and then fixed (middle panels). Scale bar = 10 μm. B. HeLa cells expressing AQP11mycFlag were fractionated as previously described [ 22 , 23 ]. Aliquots were resolved electrophoretically and stripes of the blots decorated with the indicated antibodies. Hom, total post-nuclear homogenates. Cyt, cytosol. MP, pure mitochondrial fraction. MAM, mitochondria-associated membranes. ER, endoplasmic reticulum.
    Figure Legend Snippet: AQP11 resides in the ER. A. HeLa transfectants expressing HaloAQP11 were co-stained with fluorescent Halo ligands, and with antibodies against calnexin (CNX) or peroxiredoxin 3 (PRX3), to decorate AQP11, the ER and mitochondria, respectively. To label glycoproteins on the plasma membrane, cells were stained with concanavalin A-FITC (ConA) in absence of cell permeabilization, and then fixed (middle panels). Scale bar = 10 μm. B. HeLa cells expressing AQP11mycFlag were fractionated as previously described [ 22 , 23 ]. Aliquots were resolved electrophoretically and stripes of the blots decorated with the indicated antibodies. Hom, total post-nuclear homogenates. Cyt, cytosol. MP, pure mitochondrial fraction. MAM, mitochondria-associated membranes. ER, endoplasmic reticulum.

    Techniques Used: Expressing, Staining

    Related Articles

    Concentration Assay:

    Article Title: Role of Lactobacillus pentosus Strain b240 and the Toll-Like Receptor 2 Axis in Peyer's Patch Dendritic Cell-Mediated Immunoglobulin A Enhancement
    Article Snippet: .. Preparation of fluorescein isothiocyanate (FITC)-labeled b240 Heat-killed b240 was suspended at a concentration of 5 mg/ml in 50 mM carbonate buffer (pH 9.6; Sigma, St. Louis, MO, USA), reacted with FITC (Sigma) at 37°C for 60 min, washed twice with sterile Dulbecco's phosphate-buffered saline (D-PBS)(-) (Nacalai Tesque, Kyoto, Japan), and finally suspended in autoclaved water. ..

    Article Title: miR-127 Protects Proximal Tubule Cells against Ischemia/Reperfusion: Identification of Kinesin Family Member 3B as miR-127 Target
    Article Snippet: Images were obtained with Spectral Confocal Microscope TCS SP5 (Leica Microsystems, Barcelona, Spain). .. Pinocytosis Assay Dextran-FITC of 70 KDa (SD70S, Sigma-Aldrich) to a final concentration of 1 mg/ml was added to culture medium 6 hours before sample collection and incubation was performed at 37 °C. .. After incubation, samples were fixed in 4% paraformaldehyde and coverslips were mounted using prolong antifade reagent (Invitrogen) with DAPI.

    Incubation:

    Article Title: miR-127 Protects Proximal Tubule Cells against Ischemia/Reperfusion: Identification of Kinesin Family Member 3B as miR-127 Target
    Article Snippet: Images were obtained with Spectral Confocal Microscope TCS SP5 (Leica Microsystems, Barcelona, Spain). .. Pinocytosis Assay Dextran-FITC of 70 KDa (SD70S, Sigma-Aldrich) to a final concentration of 1 mg/ml was added to culture medium 6 hours before sample collection and incubation was performed at 37 °C. .. After incubation, samples were fixed in 4% paraformaldehyde and coverslips were mounted using prolong antifade reagent (Invitrogen) with DAPI.

    Thin Layer Chromatography:

    Article Title: TAT peptide on the surface of liposomes affords their efficient intracellular delivery even at low temperature and in the presence of metabolic inhibitors
    Article Snippet: Egg phosphatidylcholine, cholesterol, PEG-PE with different sizes of PEG units (PEG2000-PE or PEG5000-PE), N -glutaryl-PE (NGPE), PE, and amphiphilic fluorescent dye rhodamine (Rh)-PE were obtained from Avanti Polar Lipids. pNP-PEG-PE was synthesized in-house. .. Diethylenetriaminepentaacetic acid anhydride (DTPA), PEG-nitophenylcarbonyl [(pNP)2 ], triethylamine, octyl glucoside, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, N -hydroxysulfosuccinimide, FITC-dextran (molecular mass, 4,400 Da), sodium azide, IAA, and TLC plates were from Sigma–Aldrich. ..

    other:

    Article Title: Subtilisin Increases Macromolecular Efflux from the Oral Mucosa
    Article Snippet: FITC-dextran, subtilisin, des-Arg9 -[Leu8 ]bradykinin, aprotinin, and indomethacin were obtained from Sigma Chemical Co. (St. Louis, Mo.).

    Double Staining:

    Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
    Article Snippet: Proteins were then transferred onto membranes and probed for IRF3 dimerization with IRF3 antibody. .. Double staining was performed with the indicated primary antibodies BGLF4 MAb 2224 ( ) and IRF3 antibody (Santa Cruz Biotechnology) at 37°C for 2 h, followed by fluorescein isothiocyanate-conjugated anti-rabbit immunoglobulin G (IgG) (1:100; Jackson) and rhodamine red-conjugated anti-mouse IgG (1:100; Jackson) antibodies at 37°C for 1 h. DNA was stained with Hoechst 33258 stain (Sigma-Aldrich) at room temperature for 30 s. Slides were mounted with medium (H1000; Vector) for fluorescence microscopy (Axioskop 40 FL; Zeiss). .. Chromatin immunoprecipitation (ChIP) assays were performed according to the protocols of Upstate Biotechnology, with minor modifications.

    Staining:

    Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
    Article Snippet: Proteins were then transferred onto membranes and probed for IRF3 dimerization with IRF3 antibody. .. Double staining was performed with the indicated primary antibodies BGLF4 MAb 2224 ( ) and IRF3 antibody (Santa Cruz Biotechnology) at 37°C for 2 h, followed by fluorescein isothiocyanate-conjugated anti-rabbit immunoglobulin G (IgG) (1:100; Jackson) and rhodamine red-conjugated anti-mouse IgG (1:100; Jackson) antibodies at 37°C for 1 h. DNA was stained with Hoechst 33258 stain (Sigma-Aldrich) at room temperature for 30 s. Slides were mounted with medium (H1000; Vector) for fluorescence microscopy (Axioskop 40 FL; Zeiss). .. Chromatin immunoprecipitation (ChIP) assays were performed according to the protocols of Upstate Biotechnology, with minor modifications.

    Plasmid Preparation:

    Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
    Article Snippet: Proteins were then transferred onto membranes and probed for IRF3 dimerization with IRF3 antibody. .. Double staining was performed with the indicated primary antibodies BGLF4 MAb 2224 ( ) and IRF3 antibody (Santa Cruz Biotechnology) at 37°C for 2 h, followed by fluorescein isothiocyanate-conjugated anti-rabbit immunoglobulin G (IgG) (1:100; Jackson) and rhodamine red-conjugated anti-mouse IgG (1:100; Jackson) antibodies at 37°C for 1 h. DNA was stained with Hoechst 33258 stain (Sigma-Aldrich) at room temperature for 30 s. Slides were mounted with medium (H1000; Vector) for fluorescence microscopy (Axioskop 40 FL; Zeiss). .. Chromatin immunoprecipitation (ChIP) assays were performed according to the protocols of Upstate Biotechnology, with minor modifications.

    Fluorescence:

    Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
    Article Snippet: Proteins were then transferred onto membranes and probed for IRF3 dimerization with IRF3 antibody. .. Double staining was performed with the indicated primary antibodies BGLF4 MAb 2224 ( ) and IRF3 antibody (Santa Cruz Biotechnology) at 37°C for 2 h, followed by fluorescein isothiocyanate-conjugated anti-rabbit immunoglobulin G (IgG) (1:100; Jackson) and rhodamine red-conjugated anti-mouse IgG (1:100; Jackson) antibodies at 37°C for 1 h. DNA was stained with Hoechst 33258 stain (Sigma-Aldrich) at room temperature for 30 s. Slides were mounted with medium (H1000; Vector) for fluorescence microscopy (Axioskop 40 FL; Zeiss). .. Chromatin immunoprecipitation (ChIP) assays were performed according to the protocols of Upstate Biotechnology, with minor modifications.

    Microscopy:

    Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
    Article Snippet: Proteins were then transferred onto membranes and probed for IRF3 dimerization with IRF3 antibody. .. Double staining was performed with the indicated primary antibodies BGLF4 MAb 2224 ( ) and IRF3 antibody (Santa Cruz Biotechnology) at 37°C for 2 h, followed by fluorescein isothiocyanate-conjugated anti-rabbit immunoglobulin G (IgG) (1:100; Jackson) and rhodamine red-conjugated anti-mouse IgG (1:100; Jackson) antibodies at 37°C for 1 h. DNA was stained with Hoechst 33258 stain (Sigma-Aldrich) at room temperature for 30 s. Slides were mounted with medium (H1000; Vector) for fluorescence microscopy (Axioskop 40 FL; Zeiss). .. Chromatin immunoprecipitation (ChIP) assays were performed according to the protocols of Upstate Biotechnology, with minor modifications.

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  • 93
    Millipore fitc conjugated con a
    Fluorescence images of spotted Man2 after treating with <t>FITC-Con</t> A. Man2 was immobilized on (a) PAAm-PFPA, and (b) PFPA-silane surface, respectively.
    Fitc Conjugated Con A, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore succinyl concanavalin a fitc
    Black and white confocal microscope images of a raphe imprint or footpad polymer of Amphora stained with concanavalin <t>A-FITC.</t> (a) There is less polymer near the middle of the footpads than that at either end because the raphes (18 μm) do not run
    Succinyl Concanavalin A Fitc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/succinyl concanavalin a fitc/product/Millipore
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    99
    Millipore fitc conjugated concanavalin a con a
    Half desmosome internalisation is cPKC and actin dependent. (A) MDCK cells cultured in NCM had desmosomes at cell-cell contacts as indicated by DP staining. (B) Internalised rings of DP were present in cells treated for 60 minutes with LCM (arrows). (All internalisation controls in LCM were carried out in the presence of the appropriate drug vehicle.) (C) Internalisation of desmosomes was prevented by co-treatment with LCM and Gö6976 (0.8 µM), as cell contact was lost but half desmosomes remained at the cell surface giving rise to the appearance of intercellular gaps (arrows). (D) In NCM, DP (red) was localised to the cell surface in association with the surface marker <t>con-A</t> (green). (E) LCM treatment caused internalisation of DP and separation from con-A, which remained at the surface. (F) Co-treatment with LCM and latrunculin A (5 µM) inhibited desmosome internalisation, as indicated by persistent association of DP with con-A. Fluorescence profiles depict the intensity of staining along the white line in the images.
    Fitc Conjugated Concanavalin A Con A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated concanavalin a con a/product/Millipore
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    99
    Millipore fluorescein isothiocyanate concanavalin a
    Effect of antibiotics on biofilm structure of B . thailandensis . CLSM images of 2-day old biofilm of B . thailandensis E264 exposed to 16×MIC of each antibiotic in MVBM (A, B) and 0.1×MVBM (C, D) for 16 h and stained with fluorescein <t>isothiocyanate-concanavalin</t> A. A representative CLSM image is shown for each sample. At higher magnification (B, D), CAZ-treated biofilm cells displayed a long filamentous change while short filaments (white arrow) and large ovoid cells (white arrow head) were observed for IMI- and MEM-treated biofilm cells similar to those of B . pseudomallei K96243. The scale bar at 100× magnification (A, C): 100 μm; the scale bar at 630× magnification (B, D): 10 μm.
    Fluorescein Isothiocyanate Concanavalin A, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fluorescence images of spotted Man2 after treating with FITC-Con A. Man2 was immobilized on (a) PAAm-PFPA, and (b) PFPA-silane surface, respectively.

    Journal: Langmuir : the ACS journal of surfaces and colloids

    Article Title: Polymer-Based Photocoupling Agent for the Efficient Immobilization of Nanomaterials and Small Molecules

    doi: 10.1021/la201324h

    Figure Lengend Snippet: Fluorescence images of spotted Man2 after treating with FITC-Con A. Man2 was immobilized on (a) PAAm-PFPA, and (b) PFPA-silane surface, respectively.

    Article Snippet: Methyl pentafluorobenzoate, sodium azide, styrene, dodecyl trimethoxysilane (DTMS), poly(2-ethyl-2-oxazoline) (PEOX, MW 500,000), poly(vinyl pyrrolidone) (PVP, MW 1,300,000), sodium dodecyl sulfate (SDS), potassium persulfate, FITC (fluorescein isothiocyanate isomer I, 90%), FITC conjugated Con A (lectin from Canavalia ensiformis (Jack bean), Type IV) (FITC-Con A), bovine serum albumin (BSA), tetraethylorthosilane (TEOS), dodecyltrimethoxysilane, and 3-glycidyloxypropyltrimethoxysilane (GOTMS) were purchased from Sigma-Aldrich.

    Techniques: Fluorescence

    Black and white confocal microscope images of a raphe imprint or footpad polymer of Amphora stained with concanavalin A-FITC. (a) There is less polymer near the middle of the footpads than that at either end because the raphes (18 μm) do not run

    Journal:

    Article Title: Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms

    doi: 10.1128/AEM.71.1.428-435.2005

    Figure Lengend Snippet: Black and white confocal microscope images of a raphe imprint or footpad polymer of Amphora stained with concanavalin A-FITC. (a) There is less polymer near the middle of the footpads than that at either end because the raphes (18 μm) do not run

    Article Snippet: The following lectins were investigated to determine their abilities to bind to diatom extracellular polymers: concanavalin A-fluorescein isothiocyanate (FITC) (lectin L1; specific for glucose and mannose; Sigma Chemical Co., St. Louis, Mo.), Bandeiraea simplicifolia lectin-FITC (lectin L2; specific for galactose and N -acetylgalactose; Sigma), Tetraconolobus purpurea lectin- FITC (lectin L3; specific for fucose; Sigma), Arachis hypogaea lectin-FITC (lectin L4; specific galactosyl- N -galactose; Sigma), succinyl concanavalin A-FITC (lectin L5; specific for glucose and mannose; Sigma), concanavalin A-Oregon Green (lectin L6; specific for glucose and mannose; Molecular Probes Inc., Eugene, Oreg.), and Phaseolus vulgaris lectin (lectin L7; specific for general oligosaccharides; Sigma).

    Techniques: Microscopy, Staining

    EPS of diatoms ( A. coffeaeformis and Navicula sp. strain 1) stained green with concanavalin A-FITC. Chloroplasts autofluoresce red. (a) The tracks of polymer were used to determine motility. There are two parallel lines of polymer (arrows) because Amphora

    Journal:

    Article Title: Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms

    doi: 10.1128/AEM.71.1.428-435.2005

    Figure Lengend Snippet: EPS of diatoms ( A. coffeaeformis and Navicula sp. strain 1) stained green with concanavalin A-FITC. Chloroplasts autofluoresce red. (a) The tracks of polymer were used to determine motility. There are two parallel lines of polymer (arrows) because Amphora

    Article Snippet: The following lectins were investigated to determine their abilities to bind to diatom extracellular polymers: concanavalin A-fluorescein isothiocyanate (FITC) (lectin L1; specific for glucose and mannose; Sigma Chemical Co., St. Louis, Mo.), Bandeiraea simplicifolia lectin-FITC (lectin L2; specific for galactose and N -acetylgalactose; Sigma), Tetraconolobus purpurea lectin- FITC (lectin L3; specific for fucose; Sigma), Arachis hypogaea lectin-FITC (lectin L4; specific galactosyl- N -galactose; Sigma), succinyl concanavalin A-FITC (lectin L5; specific for glucose and mannose; Sigma), concanavalin A-Oregon Green (lectin L6; specific for glucose and mannose; Molecular Probes Inc., Eugene, Oreg.), and Phaseolus vulgaris lectin (lectin L7; specific for general oligosaccharides; Sigma).

    Techniques: Staining

    Confocal micrograph of a colony of Navicula sp. strain D stained with concanavalin A-FITC. (a) Colony seen from above. The red areas are the chloroplasts in cells, and the green-yellow areas are the polymer matrix. (b) Image identical to panel a but with

    Journal:

    Article Title: Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms

    doi: 10.1128/AEM.71.1.428-435.2005

    Figure Lengend Snippet: Confocal micrograph of a colony of Navicula sp. strain D stained with concanavalin A-FITC. (a) Colony seen from above. The red areas are the chloroplasts in cells, and the green-yellow areas are the polymer matrix. (b) Image identical to panel a but with

    Article Snippet: The following lectins were investigated to determine their abilities to bind to diatom extracellular polymers: concanavalin A-fluorescein isothiocyanate (FITC) (lectin L1; specific for glucose and mannose; Sigma Chemical Co., St. Louis, Mo.), Bandeiraea simplicifolia lectin-FITC (lectin L2; specific for galactose and N -acetylgalactose; Sigma), Tetraconolobus purpurea lectin- FITC (lectin L3; specific for fucose; Sigma), Arachis hypogaea lectin-FITC (lectin L4; specific galactosyl- N -galactose; Sigma), succinyl concanavalin A-FITC (lectin L5; specific for glucose and mannose; Sigma), concanavalin A-Oregon Green (lectin L6; specific for glucose and mannose; Molecular Probes Inc., Eugene, Oreg.), and Phaseolus vulgaris lectin (lectin L7; specific for general oligosaccharides; Sigma).

    Techniques: Staining

    Half desmosome internalisation is cPKC and actin dependent. (A) MDCK cells cultured in NCM had desmosomes at cell-cell contacts as indicated by DP staining. (B) Internalised rings of DP were present in cells treated for 60 minutes with LCM (arrows). (All internalisation controls in LCM were carried out in the presence of the appropriate drug vehicle.) (C) Internalisation of desmosomes was prevented by co-treatment with LCM and Gö6976 (0.8 µM), as cell contact was lost but half desmosomes remained at the cell surface giving rise to the appearance of intercellular gaps (arrows). (D) In NCM, DP (red) was localised to the cell surface in association with the surface marker con-A (green). (E) LCM treatment caused internalisation of DP and separation from con-A, which remained at the surface. (F) Co-treatment with LCM and latrunculin A (5 µM) inhibited desmosome internalisation, as indicated by persistent association of DP with con-A. Fluorescence profiles depict the intensity of staining along the white line in the images.

    Journal: PLoS ONE

    Article Title: Down-Regulation of Desmosomes in Cultured Cells: The Roles of PKC, Microtubules and Lysosomal/Proteasomal Degradation

    doi: 10.1371/journal.pone.0108570

    Figure Lengend Snippet: Half desmosome internalisation is cPKC and actin dependent. (A) MDCK cells cultured in NCM had desmosomes at cell-cell contacts as indicated by DP staining. (B) Internalised rings of DP were present in cells treated for 60 minutes with LCM (arrows). (All internalisation controls in LCM were carried out in the presence of the appropriate drug vehicle.) (C) Internalisation of desmosomes was prevented by co-treatment with LCM and Gö6976 (0.8 µM), as cell contact was lost but half desmosomes remained at the cell surface giving rise to the appearance of intercellular gaps (arrows). (D) In NCM, DP (red) was localised to the cell surface in association with the surface marker con-A (green). (E) LCM treatment caused internalisation of DP and separation from con-A, which remained at the surface. (F) Co-treatment with LCM and latrunculin A (5 µM) inhibited desmosome internalisation, as indicated by persistent association of DP with con-A. Fluorescence profiles depict the intensity of staining along the white line in the images.

    Article Snippet: Immunoflourescence and image processing For plasma membrane staining, cells were rinsed in ice-cold PBS and incubated with 40 µg/ml FITC-conjugated concanavalin A (con-A) (Sigma) for 30 minutes at 4°C prior to fixation.

    Techniques: Cell Culture, Staining, Laser Capture Microdissection, Marker, Fluorescence

    Effect of antibiotics on biofilm structure of B . thailandensis . CLSM images of 2-day old biofilm of B . thailandensis E264 exposed to 16×MIC of each antibiotic in MVBM (A, B) and 0.1×MVBM (C, D) for 16 h and stained with fluorescein isothiocyanate-concanavalin A. A representative CLSM image is shown for each sample. At higher magnification (B, D), CAZ-treated biofilm cells displayed a long filamentous change while short filaments (white arrow) and large ovoid cells (white arrow head) were observed for IMI- and MEM-treated biofilm cells similar to those of B . pseudomallei K96243. The scale bar at 100× magnification (A, C): 100 μm; the scale bar at 630× magnification (B, D): 10 μm.

    Journal: PLoS ONE

    Article Title: Impact of nutritional stress on drug susceptibility and biofilm structures of Burkholderia pseudomallei and Burkholderia thailandensis grown in static and microfluidic systems

    doi: 10.1371/journal.pone.0194946

    Figure Lengend Snippet: Effect of antibiotics on biofilm structure of B . thailandensis . CLSM images of 2-day old biofilm of B . thailandensis E264 exposed to 16×MIC of each antibiotic in MVBM (A, B) and 0.1×MVBM (C, D) for 16 h and stained with fluorescein isothiocyanate-concanavalin A. A representative CLSM image is shown for each sample. At higher magnification (B, D), CAZ-treated biofilm cells displayed a long filamentous change while short filaments (white arrow) and large ovoid cells (white arrow head) were observed for IMI- and MEM-treated biofilm cells similar to those of B . pseudomallei K96243. The scale bar at 100× magnification (A, C): 100 μm; the scale bar at 630× magnification (B, D): 10 μm.

    Article Snippet: The extracellular polymeric substance of the biofilm was stained with fluorescein isothiocyanate-concanavalin A (Sigma, Sigma-Aldrich, MO) for 5 min and washed with water to remove excess dye.

    Techniques: Confocal Laser Scanning Microscopy, Staining

    Effect of antibiotics on biofilm structure of B . pseudomallei . CLSM images of 2-day old biofilm of B . pseudomallei K96243 exposed to 16×MIC of each antibiotic in MVBM (A, B) and 0.1×MVBM (C, D) for 16 h and stained with fluorescein isothiocyanate-concanavalin A. A representative CLSM image is shown for each sample. At higher magnification (B, D), CAZ-treated biofilm cells displayed a long filamentous change while short filaments (white arrow) and large ovoid cells (white arrow head) were observed for IMI- and MEM-treated biofilm cells in 0.1×MVBM. The scale bar at 100× magnification (A, C): 100 μm; the scale bar at 630× magnification (B, D): 10 μm.

    Journal: PLoS ONE

    Article Title: Impact of nutritional stress on drug susceptibility and biofilm structures of Burkholderia pseudomallei and Burkholderia thailandensis grown in static and microfluidic systems

    doi: 10.1371/journal.pone.0194946

    Figure Lengend Snippet: Effect of antibiotics on biofilm structure of B . pseudomallei . CLSM images of 2-day old biofilm of B . pseudomallei K96243 exposed to 16×MIC of each antibiotic in MVBM (A, B) and 0.1×MVBM (C, D) for 16 h and stained with fluorescein isothiocyanate-concanavalin A. A representative CLSM image is shown for each sample. At higher magnification (B, D), CAZ-treated biofilm cells displayed a long filamentous change while short filaments (white arrow) and large ovoid cells (white arrow head) were observed for IMI- and MEM-treated biofilm cells in 0.1×MVBM. The scale bar at 100× magnification (A, C): 100 μm; the scale bar at 630× magnification (B, D): 10 μm.

    Article Snippet: The extracellular polymeric substance of the biofilm was stained with fluorescein isothiocyanate-concanavalin A (Sigma, Sigma-Aldrich, MO) for 5 min and washed with water to remove excess dye.

    Techniques: Confocal Laser Scanning Microscopy, Staining