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PrimerDesign Inc complimentary dna cdna
Complimentary Dna Cdna, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complimentary dna cdna/product/PrimerDesign Inc
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
complimentary dna cdna - by Bioz Stars, 2020-08
90/100 stars

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Article Title: Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion
Article Snippet: Complimentary DNA (cDNA) was synthesised according to kit manufacturer protocols (PrimerDesign Ltd, UK).

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  • 92
    PrimerDesign Inc cdna synthesis
    Generation of recq2 null mutants in bloodstream form T. brucei . ( A ) PCR was used to amplify a 5’ and a 3’ region (blue arrows) of the RECQ2 ORF (orange arrow). These PCR products were the ‘targeting regions’ cloned into the constructs shown below,which weremade to generate heterozygous (+/-) and knockout (-/-) mutants by replacing the ORF with resistance cassettes. Generalised representation of the linearised blasticidin and neomycin (G418) resistance constructs relative to the RECQ2 ORF (orange) and the targeting flanking regions (UTR; grey), which allow HR-mediated exchange (crosses). β/α tub, β/ α tubulin intergenic region; actin IR, actin intergenic region; BSD , blasticidin resistance; NEO , neomycin (G418) resistance. Not to scale. ( B ) The upper diagram shows diagnostic PCRs to confirm replacement of RECQ2 with the knockout constructs (positions of primers are indicted by arrows): a region of the ORF was amplified (“ORF PCR”) and, in addition, a region was amplified using a forward primer lying upstream of the 5’ UTR region in the knockout construct and a reverse primer specific to the BSD or NEO genes (“BSD PCR” and “NEO PCR”, respectively). The lower diagram shows agarose gels of PCR products generated from genomic DNA from wild type (WT) cells and heterozygote (+/-) and knockout (-/-) clones using primers described above. Distilled water was used as a negative control (-). Broken lines indicate different images aligned in this figure; size markers are shown (bp). ( C ). RT-PCR confirmation of recq2 mutants. A region of the PIF6 ORF and a region of the RECQ2 ORF was PCR-amplified using <t>cDNA</t> (+RT) synthesised from <t>RNA</t> extracted from wild type (wt) cells and from recq2 +/- and recq2 -/- mutants; control samples in which no reverse transcriptase had been added (-RT) are shown, as are controls using distilled water rather than substrate (-); size markers on the agarose gels are shown (bp). DOI: http://dx.doi.org/10.7554/eLife.12765.004
    Cdna Synthesis, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis/product/PrimerDesign Inc
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    PrimerDesign Inc cdna templates
    PCR amplification products of the candidate reference genes on <t>cDNA</t> and <t>gDNA</t> templates. “c” represents the cDNA template. “g” represents the gDNA template.
    Cdna Templates, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna templates/product/PrimerDesign Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna templates - by Bioz Stars, 2020-08
    92/100 stars
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    85
    PrimerDesign Inc mecp2 cdna plasmid
    Functional characterization of <t>MeCP2</t> and MECP2 mutations on MET transcriptional activation. ( a ) Luciferase reporter assays demonstrate differential activation of the MET promoter by MeCP2. MET luciferase reporter constructs containing rs1858830 G or C were transiently transfected into HEK cells with or without addition of MECP2 <t>cDNA.</t> * P
    Mecp2 Cdna Plasmid, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mecp2 cdna plasmid/product/PrimerDesign Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mecp2 cdna plasmid - by Bioz Stars, 2020-08
    85/100 stars
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    Generation of recq2 null mutants in bloodstream form T. brucei . ( A ) PCR was used to amplify a 5’ and a 3’ region (blue arrows) of the RECQ2 ORF (orange arrow). These PCR products were the ‘targeting regions’ cloned into the constructs shown below,which weremade to generate heterozygous (+/-) and knockout (-/-) mutants by replacing the ORF with resistance cassettes. Generalised representation of the linearised blasticidin and neomycin (G418) resistance constructs relative to the RECQ2 ORF (orange) and the targeting flanking regions (UTR; grey), which allow HR-mediated exchange (crosses). β/α tub, β/ α tubulin intergenic region; actin IR, actin intergenic region; BSD , blasticidin resistance; NEO , neomycin (G418) resistance. Not to scale. ( B ) The upper diagram shows diagnostic PCRs to confirm replacement of RECQ2 with the knockout constructs (positions of primers are indicted by arrows): a region of the ORF was amplified (“ORF PCR”) and, in addition, a region was amplified using a forward primer lying upstream of the 5’ UTR region in the knockout construct and a reverse primer specific to the BSD or NEO genes (“BSD PCR” and “NEO PCR”, respectively). The lower diagram shows agarose gels of PCR products generated from genomic DNA from wild type (WT) cells and heterozygote (+/-) and knockout (-/-) clones using primers described above. Distilled water was used as a negative control (-). Broken lines indicate different images aligned in this figure; size markers are shown (bp). ( C ). RT-PCR confirmation of recq2 mutants. A region of the PIF6 ORF and a region of the RECQ2 ORF was PCR-amplified using cDNA (+RT) synthesised from RNA extracted from wild type (wt) cells and from recq2 +/- and recq2 -/- mutants; control samples in which no reverse transcriptase had been added (-RT) are shown, as are controls using distilled water rather than substrate (-); size markers on the agarose gels are shown (bp). DOI: http://dx.doi.org/10.7554/eLife.12765.004

    Journal: eLife

    Article Title: Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation

    doi: 10.7554/eLife.12765

    Figure Lengend Snippet: Generation of recq2 null mutants in bloodstream form T. brucei . ( A ) PCR was used to amplify a 5’ and a 3’ region (blue arrows) of the RECQ2 ORF (orange arrow). These PCR products were the ‘targeting regions’ cloned into the constructs shown below,which weremade to generate heterozygous (+/-) and knockout (-/-) mutants by replacing the ORF with resistance cassettes. Generalised representation of the linearised blasticidin and neomycin (G418) resistance constructs relative to the RECQ2 ORF (orange) and the targeting flanking regions (UTR; grey), which allow HR-mediated exchange (crosses). β/α tub, β/ α tubulin intergenic region; actin IR, actin intergenic region; BSD , blasticidin resistance; NEO , neomycin (G418) resistance. Not to scale. ( B ) The upper diagram shows diagnostic PCRs to confirm replacement of RECQ2 with the knockout constructs (positions of primers are indicted by arrows): a region of the ORF was amplified (“ORF PCR”) and, in addition, a region was amplified using a forward primer lying upstream of the 5’ UTR region in the knockout construct and a reverse primer specific to the BSD or NEO genes (“BSD PCR” and “NEO PCR”, respectively). The lower diagram shows agarose gels of PCR products generated from genomic DNA from wild type (WT) cells and heterozygote (+/-) and knockout (-/-) clones using primers described above. Distilled water was used as a negative control (-). Broken lines indicate different images aligned in this figure; size markers are shown (bp). ( C ). RT-PCR confirmation of recq2 mutants. A region of the PIF6 ORF and a region of the RECQ2 ORF was PCR-amplified using cDNA (+RT) synthesised from RNA extracted from wild type (wt) cells and from recq2 +/- and recq2 -/- mutants; control samples in which no reverse transcriptase had been added (-RT) are shown, as are controls using distilled water rather than substrate (-); size markers on the agarose gels are shown (bp). DOI: http://dx.doi.org/10.7554/eLife.12765.004

    Article Snippet: RNA was extracted from the cells using the Qiagen RNeasy kit, and cDNA synthesis was performed using random primers and the Primer Design Precision nanoScript Reverse Transcription kit (Primer Design), according to manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Clone Assay, Construct, Knock-Out, Diagnostic Assay, Amplification, Generated, Negative Control, Reverse Transcription Polymerase Chain Reaction

    Generation of TbRECQ2 mutants in T. brucei HR1 and HRES cells. ( A ) Agarose gels of PCR products generated from genomic DNA from HRES or HR1 wild type (WT) cells and RECQ2 heterozygote (+/-) and knockout (-/-) clones using primers described in the Figure S1B . Gaps indicate that lanes have been aligned in this figure after excision from multiple gels or from disparate parts of the same gel; size markers are shown (bp). ( B ) Confirmation by PCR of RECQ2 knockout in HR1 and HRES cell lines. Agarose gels showing products generated when PCR was performed on a region of the PIF6 ORF or the RECQ2 ORF using cDNA (+RT) synthesised from RNA extracted from recq2 -/- mutants; control reactions in which no substrate was added (-), or where cDNA from HR1 WT cells was used, are shown. Gaps indicate that lanes have been aligned in this figure after excision from multiple gels or from disparate parts of the same gel; size markers are shown (bp). DOI: http://dx.doi.org/10.7554/eLife.12765.007

    Journal: eLife

    Article Title: Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation

    doi: 10.7554/eLife.12765

    Figure Lengend Snippet: Generation of TbRECQ2 mutants in T. brucei HR1 and HRES cells. ( A ) Agarose gels of PCR products generated from genomic DNA from HRES or HR1 wild type (WT) cells and RECQ2 heterozygote (+/-) and knockout (-/-) clones using primers described in the Figure S1B . Gaps indicate that lanes have been aligned in this figure after excision from multiple gels or from disparate parts of the same gel; size markers are shown (bp). ( B ) Confirmation by PCR of RECQ2 knockout in HR1 and HRES cell lines. Agarose gels showing products generated when PCR was performed on a region of the PIF6 ORF or the RECQ2 ORF using cDNA (+RT) synthesised from RNA extracted from recq2 -/- mutants; control reactions in which no substrate was added (-), or where cDNA from HR1 WT cells was used, are shown. Gaps indicate that lanes have been aligned in this figure after excision from multiple gels or from disparate parts of the same gel; size markers are shown (bp). DOI: http://dx.doi.org/10.7554/eLife.12765.007

    Article Snippet: RNA was extracted from the cells using the Qiagen RNeasy kit, and cDNA synthesis was performed using random primers and the Primer Design Precision nanoScript Reverse Transcription kit (Primer Design), according to manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Generated, Knock-Out, Clone Assay

    Phylogenetic relationships among SOD1s of various organisms reconstructed on the basis of the cDNA coding region sequences and using both Bayesian interference (BI) and maximum likelihood (ML) methods. Bayesian posterior probability (first number) and bootstrap values higher than 50% are indicated on each node, respectively. The scale for branch length (1.1 substitution/site) is shown below the tree. C. hamatus SOD1 is boxed.

    Journal: Antioxidants

    Article Title: Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

    doi: 10.3390/antiox9040325

    Figure Lengend Snippet: Phylogenetic relationships among SOD1s of various organisms reconstructed on the basis of the cDNA coding region sequences and using both Bayesian interference (BI) and maximum likelihood (ML) methods. Bayesian posterior probability (first number) and bootstrap values higher than 50% are indicated on each node, respectively. The scale for branch length (1.1 substitution/site) is shown below the tree. C. hamatus SOD1 is boxed.

    Article Snippet: Primers Design, RNA Extraction, cDNA Synthesis, RACE, Cloning, and Sequencing of SOD1 mRNA Amino acid and nucleotide sequences of SOD1 from notothenioid fish obtained from the NCBI database were aligned by MUSCLE to identify conserved domains for primer design [ ].

    Techniques:

    PCR amplification products of the candidate reference genes on cDNA and gDNA templates. “c” represents the cDNA template. “g” represents the gDNA template.

    Journal: PLoS ONE

    Article Title: Evaluation of Appropriate Reference Genes for Gene Expression Normalization during Watermelon Fruit Development

    doi: 10.1371/journal.pone.0130865

    Figure Lengend Snippet: PCR amplification products of the candidate reference genes on cDNA and gDNA templates. “c” represents the cDNA template. “g” represents the gDNA template.

    Article Snippet: Each reference gene amplified a specific product on cDNA templates, but no products were detected on gDNA templates except for ClACT and Cl18SrRNA , demonstrating the success of primer design ( ).

    Techniques: Polymerase Chain Reaction, Amplification

    Functional characterization of MeCP2 and MECP2 mutations on MET transcriptional activation. ( a ) Luciferase reporter assays demonstrate differential activation of the MET promoter by MeCP2. MET luciferase reporter constructs containing rs1858830 G or C were transiently transfected into HEK cells with or without addition of MECP2 cDNA. * P

    Journal: Translational Psychiatry

    Article Title: Transcriptional regulation of the MET receptor tyrosine kinase gene by MeCP2 and sex-specific expression in autism and Rett syndrome

    doi: 10.1038/tp.2013.91

    Figure Lengend Snippet: Functional characterization of MeCP2 and MECP2 mutations on MET transcriptional activation. ( a ) Luciferase reporter assays demonstrate differential activation of the MET promoter by MeCP2. MET luciferase reporter constructs containing rs1858830 G or C were transiently transfected into HEK cells with or without addition of MECP2 cDNA. * P

    Article Snippet: We utilized the MeCP2 cDNA plasmid as template and followed the manufacturer's primer design software (Stratagene).

    Techniques: Functional Assay, Activation Assay, Luciferase, Construct, Transfection