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    Name:
    COX IV D6I4K Rabbit mAb Rodent Specific
    Description:
    Cytochrome c oxidase COX is a hetero oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane 1 3 It is the terminal enzyme complex in the respiratory chain catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits including COX IV are nuclear encoded Research studies have shown that deficiency in COX activity correlates with a number of human diseases 4 The COX IV antibody can be used effectively as a mitochondrial loading control in cell based research assays
    Catalog Number:
    38563
    Price:
    None
    Applications:
    Western Blot, Immunoprecipitation, Immunohistochemistry
    Category:
    Primary Antibodies
    Source:
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp171 of mouse COX IV protein.
    Reactivity:
    Mouse Rat
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    Structured Review

    Cell Signaling Technology Inc complex iv
    Cytochrome c oxidase COX is a hetero oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane 1 3 It is the terminal enzyme complex in the respiratory chain catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits including COX IV are nuclear encoded Research studies have shown that deficiency in COX activity correlates with a number of human diseases 4 The COX IV antibody can be used effectively as a mitochondrial loading control in cell based research assays
    https://www.bioz.com/result/complex iv/product/Cell Signaling Technology Inc
    Average 97 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    complex iv - by Bioz Stars, 2020-07
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies
    Article Snippet: .. The cell staining procedures were performed using the following antibodies as previously described [ ]: mouse monoclonal anti-PHB1 (abcam), affinity purified rabbit polyclonal anti-PHB2 [ ], mouse monoclonal anti-OxPhos CII (Invitrogen), or rabbit monoclonal anti-COX IV (cell signaling). .. To reduce photobleaching effects, all staining steps were executed in the dark.

    Affinity Purification:

    Article Title: The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies
    Article Snippet: .. The cell staining procedures were performed using the following antibodies as previously described [ ]: mouse monoclonal anti-PHB1 (abcam), affinity purified rabbit polyclonal anti-PHB2 [ ], mouse monoclonal anti-OxPhos CII (Invitrogen), or rabbit monoclonal anti-COX IV (cell signaling). .. To reduce photobleaching effects, all staining steps were executed in the dark.

    Western Blot:

    Article Title: NOXA as critical mediator for drug combinations in polychemotherapy
    Article Snippet: .. For western blot analysis, the following antibodies were used: anti AIF, anti BAK, anti BAX, anti BCL-2, anti cIAP-2, anti Histone H1, anti-Mcl-1 and anti p53 from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA); anti BCL-XL, anti BID, anti cleaved caspase-3, 6 and 7, anti cleaved PARP, anti cytochrome c , anti COX IV, anti Endonuclease G, anti OMI/HtrA2, anti PUMA and anti Survivin from Cell Signaling Technology Inc. (Danvers, MA, USA); anti α-Tubulin from Oncogene (San Diego, CA, USA); anti caspase-9 and anti XIAP from BD Biosciences (San Jose, SA, USA); anti GAPDH from Thermo Fisher (Waltham, MA, USA); anti NOXA from Calbiochem; anti BIM and anti caspase-8 from Alexis Corp. (Lausen, Switzerland); anti SMAC/DIABLO from Millipore (Billerica, MA, USA). ..

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  • 85
    Cell Signaling Technology Inc cytochrome c oxidase complex subunit iv
    Muscle oxidative metabolism in wild-type (WT, Wt) and Apc Min/+ mice with varying severity of cachexia. A : ratio of mitochondrial and nuclear DNA from either the red or white portion of the gastrocnemius muscle. Data are normalized to WT red gastrocnemius. R Gas, red gastrocnemius muscle; W Gas, white gastrocnemius muscle; ME, main effect. B, top : representative Western blot of <t>cytochrome</t> c protein expression in red and white portions of the gastrocnemius and soleus muscle. Bottom : cytochrome c protein expression that was normalized to the WT red gastrocnemius. IOD, integrated optical density. C, top: representative Western blot of cytochrome- c oxidase complex subunit IV (Cox IV) protein expression, in red and white portions of the gastrocnemius and soleus muscle. Bottom : Cox IV protein expression normalized to WT red gastrocnemius muscle. D : relationship between red gastrocnemius mitochondrial and nuclear DNA ratio and % body weight loss in Apc Min/+ mice ( n = 10). E : succinate dehydrogenase (SDH) staining in whole gastrocnemius muscle. SDH data are presented as % positively stained muscle fibers. Values are means ± SE. *Difference within muscle type; †different from all other severe groups; difference between red and white gastrocnemius within WT groups; @difference between the red gastrocnemius portion and the soleus muscle within the WT group. Mild, Apc Min/+ mice having mild cachexia symptoms; Severe, Apc Min/+ mice having severe cachexia symptoms.
    Cytochrome C Oxidase Complex Subunit Iv, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytochrome c oxidase complex subunit iv/product/Cell Signaling Technology Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytochrome c oxidase complex subunit iv - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    91
    Cell Signaling Technology Inc complex iv
    Muscle oxidative metabolism in wild-type (WT, Wt) and Apc Min/+ mice with varying severity of cachexia. A : ratio of mitochondrial and nuclear DNA from either the red or white portion of the gastrocnemius muscle. Data are normalized to WT red gastrocnemius. R Gas, red gastrocnemius muscle; W Gas, white gastrocnemius muscle; ME, main effect. B, top : representative Western blot of <t>cytochrome</t> c protein expression in red and white portions of the gastrocnemius and soleus muscle. Bottom : cytochrome c protein expression that was normalized to the WT red gastrocnemius. IOD, integrated optical density. C, top: representative Western blot of cytochrome- c oxidase complex subunit IV (Cox IV) protein expression, in red and white portions of the gastrocnemius and soleus muscle. Bottom : Cox IV protein expression normalized to WT red gastrocnemius muscle. D : relationship between red gastrocnemius mitochondrial and nuclear DNA ratio and % body weight loss in Apc Min/+ mice ( n = 10). E : succinate dehydrogenase (SDH) staining in whole gastrocnemius muscle. SDH data are presented as % positively stained muscle fibers. Values are means ± SE. *Difference within muscle type; †different from all other severe groups; difference between red and white gastrocnemius within WT groups; @difference between the red gastrocnemius portion and the soleus muscle within the WT group. Mild, Apc Min/+ mice having mild cachexia symptoms; Severe, Apc Min/+ mice having severe cachexia symptoms.
    Complex Iv, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complex iv/product/Cell Signaling Technology Inc
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    complex iv - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc anti cytochrome c oxidase complex iv
    Muscle oxidative metabolism in wild-type (WT, Wt) and Apc Min/+ mice with varying severity of cachexia. A : ratio of mitochondrial and nuclear DNA from either the red or white portion of the gastrocnemius muscle. Data are normalized to WT red gastrocnemius. R Gas, red gastrocnemius muscle; W Gas, white gastrocnemius muscle; ME, main effect. B, top : representative Western blot of <t>cytochrome</t> c protein expression in red and white portions of the gastrocnemius and soleus muscle. Bottom : cytochrome c protein expression that was normalized to the WT red gastrocnemius. IOD, integrated optical density. C, top: representative Western blot of cytochrome- c oxidase complex subunit IV (Cox IV) protein expression, in red and white portions of the gastrocnemius and soleus muscle. Bottom : Cox IV protein expression normalized to WT red gastrocnemius muscle. D : relationship between red gastrocnemius mitochondrial and nuclear DNA ratio and % body weight loss in Apc Min/+ mice ( n = 10). E : succinate dehydrogenase (SDH) staining in whole gastrocnemius muscle. SDH data are presented as % positively stained muscle fibers. Values are means ± SE. *Difference within muscle type; †different from all other severe groups; difference between red and white gastrocnemius within WT groups; @difference between the red gastrocnemius portion and the soleus muscle within the WT group. Mild, Apc Min/+ mice having mild cachexia symptoms; Severe, Apc Min/+ mice having severe cachexia symptoms.
    Anti Cytochrome C Oxidase Complex Iv, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cytochrome c oxidase complex iv/product/Cell Signaling Technology Inc
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti cytochrome c oxidase complex iv - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Muscle oxidative metabolism in wild-type (WT, Wt) and Apc Min/+ mice with varying severity of cachexia. A : ratio of mitochondrial and nuclear DNA from either the red or white portion of the gastrocnemius muscle. Data are normalized to WT red gastrocnemius. R Gas, red gastrocnemius muscle; W Gas, white gastrocnemius muscle; ME, main effect. B, top : representative Western blot of cytochrome c protein expression in red and white portions of the gastrocnemius and soleus muscle. Bottom : cytochrome c protein expression that was normalized to the WT red gastrocnemius. IOD, integrated optical density. C, top: representative Western blot of cytochrome- c oxidase complex subunit IV (Cox IV) protein expression, in red and white portions of the gastrocnemius and soleus muscle. Bottom : Cox IV protein expression normalized to WT red gastrocnemius muscle. D : relationship between red gastrocnemius mitochondrial and nuclear DNA ratio and % body weight loss in Apc Min/+ mice ( n = 10). E : succinate dehydrogenase (SDH) staining in whole gastrocnemius muscle. SDH data are presented as % positively stained muscle fibers. Values are means ± SE. *Difference within muscle type; †different from all other severe groups; difference between red and white gastrocnemius within WT groups; @difference between the red gastrocnemius portion and the soleus muscle within the WT group. Mild, Apc Min/+ mice having mild cachexia symptoms; Severe, Apc Min/+ mice having severe cachexia symptoms.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Muscle oxidative capacity during IL-6-dependent cancer cachexia

    doi: 10.1152/ajpregu.00300.2010

    Figure Lengend Snippet: Muscle oxidative metabolism in wild-type (WT, Wt) and Apc Min/+ mice with varying severity of cachexia. A : ratio of mitochondrial and nuclear DNA from either the red or white portion of the gastrocnemius muscle. Data are normalized to WT red gastrocnemius. R Gas, red gastrocnemius muscle; W Gas, white gastrocnemius muscle; ME, main effect. B, top : representative Western blot of cytochrome c protein expression in red and white portions of the gastrocnemius and soleus muscle. Bottom : cytochrome c protein expression that was normalized to the WT red gastrocnemius. IOD, integrated optical density. C, top: representative Western blot of cytochrome- c oxidase complex subunit IV (Cox IV) protein expression, in red and white portions of the gastrocnemius and soleus muscle. Bottom : Cox IV protein expression normalized to WT red gastrocnemius muscle. D : relationship between red gastrocnemius mitochondrial and nuclear DNA ratio and % body weight loss in Apc Min/+ mice ( n = 10). E : succinate dehydrogenase (SDH) staining in whole gastrocnemius muscle. SDH data are presented as % positively stained muscle fibers. Values are means ± SE. *Difference within muscle type; †different from all other severe groups; difference between red and white gastrocnemius within WT groups; @difference between the red gastrocnemius portion and the soleus muscle within the WT group. Mild, Apc Min/+ mice having mild cachexia symptoms; Severe, Apc Min/+ mice having severe cachexia symptoms.

    Article Snippet: Primary antibodies for cytochrome- c oxidase complex subunit IV (Cox IV; 17 kDa), cytochrome c (14 kDa), Stat3, phospho Stat3 (86 kDa) (Cell Signaling), PGC-1α (90 kDa) (Santa Cruz Biotechnology), UCP3 (33 kDa) (Thermo Scientific), 4-hydroxynonenal (4-HNE; Alpha Diagnostic), Sirt1 (120 kDa) (Santa Cruz Biotechnology), and nitrotyrosine (Sigma) were diluted 1:500 to 1:1,000 in 5% milk in TBS-T followed by a 1-h incubation at room temperature.

    Techniques: Mouse Assay, Western Blot, Expressing, Staining

    Circulating IL-6, muscle IL-6 mRNA expression, and muscle STAT3 signaling in WT and Apc Min/+ mice with varying cachexia severity. A : plasma IL-6 levels at 10, 14, and 20 wk of age in Apc Min/+ mice exhibiting mild or severe cachexia at 20 wk of age. B : correlation between plasma IL-6 level and body weight at 20 wk ( n = 9). C : correlation between large polyps and plasma IL-6 ( n = 14). D, top : representative Western blot of phosphorylated Stat3 (pStat3; TYR 705) and total Stat3 protein expression in red gastrocnemius, white gastrocnemius, and soleus muscles from WT and Apc Min/+ mice. Bottom : pStat3-to-Stat3 ratio normalized to WT red gastrocnemius muscle. E : IL-6 mRNA expression in red gastrocnemius, white gastrocnemius, and soleus muscles from WT and Apc Min/+ mice. Data are normalized to WT red gastrocnemius. F : effect of circulating IL-6 overexpression on gastrocnemius muscle oxidative (OXPHOS) protein expression in Apc Min/+ mice. Top : representative Western blot of PGC-1, Cox IV, and cytochrome c . Bottom: PGC-1, Cox IV, and cytochrome c protein expression normalized to vector control. G : effect of circulating IL-6 overexpression on gastrocnemius muscle oxidative protein expression in WT mice. Top: representative Western blot of PGC-1, Cox IV, and cytochrome c . Bottom : PGC-1, Cox IV, and cytochrome c protein expression normalized to vector control. Values are means ± SE. ND, not detectable; *different from mild group; †different from all other weeks within the severely cachectic groups; $different from control vector.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Muscle oxidative capacity during IL-6-dependent cancer cachexia

    doi: 10.1152/ajpregu.00300.2010

    Figure Lengend Snippet: Circulating IL-6, muscle IL-6 mRNA expression, and muscle STAT3 signaling in WT and Apc Min/+ mice with varying cachexia severity. A : plasma IL-6 levels at 10, 14, and 20 wk of age in Apc Min/+ mice exhibiting mild or severe cachexia at 20 wk of age. B : correlation between plasma IL-6 level and body weight at 20 wk ( n = 9). C : correlation between large polyps and plasma IL-6 ( n = 14). D, top : representative Western blot of phosphorylated Stat3 (pStat3; TYR 705) and total Stat3 protein expression in red gastrocnemius, white gastrocnemius, and soleus muscles from WT and Apc Min/+ mice. Bottom : pStat3-to-Stat3 ratio normalized to WT red gastrocnemius muscle. E : IL-6 mRNA expression in red gastrocnemius, white gastrocnemius, and soleus muscles from WT and Apc Min/+ mice. Data are normalized to WT red gastrocnemius. F : effect of circulating IL-6 overexpression on gastrocnemius muscle oxidative (OXPHOS) protein expression in Apc Min/+ mice. Top : representative Western blot of PGC-1, Cox IV, and cytochrome c . Bottom: PGC-1, Cox IV, and cytochrome c protein expression normalized to vector control. G : effect of circulating IL-6 overexpression on gastrocnemius muscle oxidative protein expression in WT mice. Top: representative Western blot of PGC-1, Cox IV, and cytochrome c . Bottom : PGC-1, Cox IV, and cytochrome c protein expression normalized to vector control. Values are means ± SE. ND, not detectable; *different from mild group; †different from all other weeks within the severely cachectic groups; $different from control vector.

    Article Snippet: Primary antibodies for cytochrome- c oxidase complex subunit IV (Cox IV; 17 kDa), cytochrome c (14 kDa), Stat3, phospho Stat3 (86 kDa) (Cell Signaling), PGC-1α (90 kDa) (Santa Cruz Biotechnology), UCP3 (33 kDa) (Thermo Scientific), 4-hydroxynonenal (4-HNE; Alpha Diagnostic), Sirt1 (120 kDa) (Santa Cruz Biotechnology), and nitrotyrosine (Sigma) were diluted 1:500 to 1:1,000 in 5% milk in TBS-T followed by a 1-h incubation at room temperature.

    Techniques: Expressing, Mouse Assay, Western Blot, Over Expression, Pyrolysis Gas Chromatography, Plasmid Preparation